################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeII MamI MboII MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcyI 107 GGCGTC G[GA]^CG[CT]C AluI 114 AGCT AG^CT AlwI 137 GGATC GGATC(4/5) Asp700I 177 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 223 GAGCAC G[AT]GC[AT]^C AspI 205 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BfaI 112 CTAG C^TAG BlpI 46 GCTAAGC GC^T[ACGT]AGC BmyI 223 GAGCAC G[AGT]GC[ACT]^C BpmI 187 CTGGAG CTGGAG(16/14) Bpu1102I 46 GCTAAGC GC^T[ACGT]AGC BsaHI 107 GGCGTC G[GA]^CG[CT]C BsaI 160 GAGACC GGTCTC(1/5) BseRI 157 GAGGAG GAGGAG(10/8) BsiHKAI 223 GAGCAC G[AT]GC[AT]^C BsiYI 54 CCACCGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 54 CCACCGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 203 GGGAC GGGAC(10/14) Bsp1286I 223 GAGCAC G[AGT]GC[ACT]^C BstF5I 255 GGATG GGATG(2/0) BstXI 251 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 137 GGATCT [GA]^GATC[CT] CelII 46 GCTAAGC GC^T[ACGT]AGC DpnII 138 GATC ^GATC EcoT14I 251 CCATGG C^C[AT][AT]GG FauI 164 CCCGC CCCGC(4/6) Fnu4HI 125 GCGGC GC^[ACGT]GC FokI 255 GGATG GGATG(9/13) HaeIII 145 GGCC GG^CC HapII 5 CCGG C^CGG Hin1I 107 GGCGTC G[GA]^CG[CT]C HinfI 198 GATTC G^A[ACGT]TC HpaII 5 CCGG C^CGG ItaI 125 GCGGC GC^[ACGT]GC KspI 165 CCGCGG CCGC^GG MaeI 112 CTAG C^TAG MboI 138 GATC ^GATC MflI 137 GGATCT [GA]^GATC[CT] MseI 131 TTAA T^TAA MspA1I 165 CCGCGG C[AC]G^C[GT]G MspI 5 CCGG C^CGG NcoI 251 CCATGG C^CATGG NdeII 138 GATC ^GATC NlaIII 252 CATG CATG^ PflFI 205 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacII 165 CCGCGG CCGC^GG Sau3AI 138 GATC ^GATC SfaNI 86 GCATC GCATC(5/9) StyI 251 CCATGG C^C[AT][AT]GG TfiI 198 GATTC G^A[AT]TC Tru9I 131 TTAA T^TAA Tth111I 205 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 137 GGATCT [GA]^GATC[CT] XmnI 177 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 166 CGCG CG^CG 267 CGCG AfaI 264 GTAC GT^AC 294 GTAC BsmAI 71 GAGAC GTCTC(1/5) 160 GAGAC BssKI 186 CCTGG ^CC[ACGT]GG 279 CCAGG BstNI 186 CCTGG CC^[AT]GG 279 CCAGG BstUI 166 CGCG CG^CG 267 CGCG Cac8I 96 GCCTGC GC[ACGT]^[ACGT]GC 225 GCACGC DdeI 30 CTTAG C^T[ACGT]AG 47 CTAAG DsaI 165 CCGCGG C^C[GA][CT]GG 251 CCATGG EcoRII 186 CCTGG ^CC[AT]GG 279 CCAGG HgaI 205 GACGC GACGC(5/10) 108 GCGTC HphI 89 TCACC GGTGA(8/7) 212 TCACC MaeIII 211 GTCAC ^GT[ACGT]AC 244 GTCAC MslI 246 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 252 CATGGATGTG MvaI 186 CCTGG CC^[AT]GG 279 CCAGG MvnI 166 CGCG CG^CG 267 CGCG RsaI 264 GTAC GT^AC 294 GTAC ScrFI 186 CCTGG CC^[ACGT]GG 279 CCAGG SmlI 66 CTTGAG C^T[CT][GA]AG 271 CTCAAG Tsp45I 211 GTCAC ^GT[GC]AC 244 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AciI BsaJI CfoI HaeII HhaI HinP1I MnlI MwoI