################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MscI MslI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TseI Tsp45I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 37 TCCGGA T^CCGGA AciI 144 GCGG CCGC(-3/-1) AocI 46 CCTGAGG CC^T[ACGT]AGG AvaI 137 CCCGGG C^[CT]CG[GA]G AvaII 234 GGACC G^G[AT]CC BamHI 81 GGATCC G^GATCC BciVI 169 GTATCC GTATCC(6/5) BfaI 148 CTAG C^TAG BpmI 7 CTGGAG CTGGAG(16/14) BsaJI 137 CCCGGG C^C[ACGT][ACGT]GG BsaWI 37 TCCGGA [AT]^CCGG[AT] BseAI 37 TCCGGA T^CCGGA BsoBI 137 CCCGGG C^[CT]CG[GA]G BspEI 37 TCCGGA T^CCGGA BsrBI 142 GAGCGG CCGCTC(-3/-3) BsrI 6 ACTGG ACTGG(1/-1) BstAPI 18 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 46 CCTGAGG CC^T[ACGT]AGG DdeI 47 CTGAG C^T[ACGT]AG Eco81I 46 CCTGAGG CC^T[ACGT]AGG Fnu4HI 144 GCGGC GC^[ACGT]GC HinfI 34 GAATC G^A[ACGT]TC ItaI 144 GCGGC GC^[ACGT]GC MaeI 148 CTAG C^TAG MroI 37 TCCGGA T^CCGGA MseI 260 TTAA T^TAA NlaIII 226 CATG CATG^ NspI 225 GCATGC [GA]CATG^[CT] PflMI 237 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfaNI 24 GATGC GCATC(5/9) SmaI 137 CCCGGG CCC^GGG SphI 225 GCATGC GCATG^C TaqI 177 TCGA T^CGA TfiI 34 GAATC G^A[AT]TC Tru9I 260 TTAA T^TAA Tsp509I 74 AATT ^AATT TspRI 95 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 177 TCGA T^CGA Van91I 237 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 137 CCCGGG C^CCGGG XmaI 137 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AluI 152 AGCT AG^CT 209 AGCT AspHI 151 GAGCTC G[AT]GC[AT]^C 208 GAGCTC BanII 151 GAGCTC G[GA]GC[CT]^C 208 GAGCTC BcnI 137 CCCGG CC^[GC]GG 138 CCGGG BmyI 151 GAGCTC G[AGT]GC[ACT]^C 208 GAGCTC BsiHKAI 151 GAGCTC G[AT]GC[AT]^C 208 GAGCTC BsiYI 137 CCCGGGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 237 CCATTGCGTGG BslI 137 CCCGGGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 237 CCATTGCGTGG BsmFI 3 GGGAC GGGAC(10/14) 233 GGGAC Bsp1286I 151 GAGCTC G[AGT]GC[ACT]^C 208 GAGCTC BsrDI 238 CATTGC GCAATG(2/0) 105 GCAATG BssKI 137 CCCGG ^CC[ACGT]GG 138 CCGGG BstYI 42 AGATCC [GA]^GATC[CT] 81 GGATCC Cac8I 110 GCGAGC GC[ACGT]^[ACGT]GC 225 GCATGC HaeIII 135 GGCC GG^CC 246 GGCC HapII 38 CCGG C^CGG 138 CCGG HpaII 38 CCGG C^CGG 138 CCGG MflI 42 AGATCC [GA]^GATC[CT] 81 GGATCC MspI 38 CCGG C^CGG 138 CCGG NciI 137 CCCGG CC^[GC]GG 138 CCGGG NlaIV 81 GGATCC GG[ACGT]^[ACGT]CC 233 GGGACC SacI 151 GAGCTC GAGCT^C 208 GAGCTC ScrFI 137 CCCGG CC^[ACGT]GG 138 CCGGG XhoII 42 AGATCC [GA]^GATC[CT] 81 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AlwI BseRI Cfr13I DpnII EarI Ksp632I MboI MboII MnlI MwoI NdeII Sau3AI Sau96I