(Circular) (Six-base) MAPSORT of: pFN21AE6174.seq Check: 9993 from: 1 to: 4978 pFN21AE6174 4978 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2788 278 Size: 53 83 186 936 1252 2468 Fragments arranged by size: 2468 1252 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2321 1937 Size: 384 4594 Acc65I G'GTAC_C Cuts at: 2299 2299 Size: 4978 AclI AA'CG_TT Cuts at: 1563 3105 3478 1563 Size: 1542 373 3063 Fragments arranged by size: 3063 1542 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3041 4425 4693 1632 Size: 1409 1384 268 1917 Fragments arranged by size: 1917 1409 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4781 AflIII A'CryG_T Cuts at: 1203 3877 4854 1203 Size: 2674 977 1327 Fragments arranged by size: 2674 1327 977 AgeI A'CCGG_T Cuts at: 3809 3809 Size: 4978 AhdI GACnn_n'nnGTC Cuts at: 3707 3707 Size: 4978 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4978 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4978 AlwNI CAG_nnn'CTG Cuts at: 2721 4293 2721 Size: 1572 3406 ApaLI G'TGCA_C Cuts at: 3035 4191 3035 Size: 1156 3822 ApoI r'AATT_y Cuts at: 1493 2287 2513 2665 4774 1493 Size: 794 226 152 2109 1697 Fragments arranged by size: 2109 1697 794 226 152 AseI AT'TA_AT Cuts at: 160 1032 3532 160 Size: 872 2500 1606 Fragments arranged by size: 2500 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4978 AvaI C'yCGr_G Cuts at: 1870 1943 2142 2303 1870 Size: 73 199 161 4545 Fragments arranged by size: 4545 199 161 73 BamHI G'GATC_C Cuts at: 2086 2308 2675 2086 Size: 222 367 4389 Fragments arranged by size: 4389 367 222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2299 3754 618 Size: 325 217 627 512 1455 1842 Fragments arranged by size: 1842 1455 627 512 325 217 BanII G_rGCy'C Cuts at: 1391 2160 2217 2297 1391 Size: 769 57 80 4072 Fragments arranged by size: 4072 769 80 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4007 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3169 3203 1903 Size: 34 1232 34 3678 Fragments arranged by size: 3678 1232 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2185 2870 4080 2185 Size: 685 1210 3083 Fragments arranged by size: 3083 1210 685 BclI T'GATC_A Cuts at: 1548 1548 Size: 4978 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3589 136 Size: 107 122 71 631 605 1917 1525 Fragments arranged by size: 1917 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4973 4973 Size: 4978 BlpI GC'TnA_GC Cuts at: 2395 2395 Size: 4978 Bme1580I G_kGCm'C Cuts at: 1790 3039 4195 1790 Size: 1249 1156 2573 Fragments arranged by size: 2573 1249 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4978 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2709 3667 442 Size: 416 53 83 399 24 1292 958 1753 Fragments arranged by size: 1753 1292 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4978 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 3638 1239 Size: 794 1605 2579 Fragments arranged by size: 2579 1605 794 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3103 3968 4266 3103 Size: 865 298 3815 Fragments arranged by size: 3815 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3641 882 Size: 617 2142 2219 Fragments arranged by size: 2219 2142 617 BsaAI yAC'GTr Cuts at: 493 1204 2769 493 Size: 711 1565 2702 Fragments arranged by size: 2702 1565 711 BsaBI GATnn'nnATC Cuts at: 4972 4972 Size: 4978 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2785 3167 3802 275 Size: 53 83 186 936 1252 382 635 1451 Fragments arranged by size: 1451 1252 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2042 3411 3809 4083 4230 4641 2042 Size: 1369 398 274 147 411 2379 Fragments arranged by size: 2379 1369 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4753 Fragments arranged by size: 4753 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2072 2087 2225 1186 Size: 883 3 15 138 3939 Fragments arranged by size: 3939 883 138 15 3 BseYI C'CCAG_C Cuts at: 4181 4677 4181 Size: 496 4482 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2428 3189 4217 1540 Size: 275 186 427 761 1028 2301 Fragments arranged by size: 2301 1028 761 427 275 186 BsiHKAI G_wGCw'C Cuts at: 2160 2217 3039 3124 4195 2160 Size: 57 822 85 1071 2943 Fragments arranged by size: 2943 1071 822 85 57 BsmI GAATG_Cn' Cuts at: 2489 2582 2489 Size: 93 4885 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2217 2297 3039 3124 4195 1391 Size: 399 370 57 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 85 80 57 BspEI T'CCGG_A Cuts at: 2042 4641 2042 Size: 2599 2379 BspHI T'CATG_A Cuts at: 2867 4597 2867 Size: 1730 3248 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2333 844 Size: 1489 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2149 2865 1394 Size: 429 326 716 3507 Fragments arranged by size: 3507 716 429 326 BsrDI GCAATG_nn' Cuts at: 65 1248 2117 2242 3473 3647 65 Size: 1183 869 125 1231 174 1396 Fragments arranged by size: 1396 1231 1183 869 174 125 BsrFI r'CCGG_y Cuts at: 1700 1810 3622 3809 1700 Size: 110 1812 187 2869 Fragments arranged by size: 2869 1812 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4978 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4978 BssSI C'ACGA_G Cuts at: 3038 4050 3038 Size: 1012 3966 BstAPI GCAn_nnn'nTGC Cuts at: 794 2029 794 Size: 1235 3743 BstBI TT'CG_AA Cuts at: 3782 3782 Size: 4978 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4978 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 4978 BstYI r'GATC_y Cuts at: 1972 2047 2086 2308 2675 3060 3077 4529 4615 Size: 75 39 222 367 385 17 1452 86 Cuts at: 4615 4627 4973 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 222 86 75 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2052 2052 Size: 4978 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3487 Fragments arranged by size: 3487 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4239 Fragments arranged by size: 4239 683 56 BtsI GCAGTG_nn' Cuts at: 2494 3288 3308 2494 Size: 794 20 4164 Fragments arranged by size: 4164 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4978 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4943 DraI TTT'AAA Cuts at: 2282 2282 Size: 4978 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3985 809 Size: 507 2669 1802 Fragments arranged by size: 2669 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2425 3314 4943 Size: 54 1425 50 275 18 595 889 1629 Cuts at: 4943 8 Size: 43 Fragments arranged by size: 1629 1425 889 595 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2425 1537 Size: 275 613 4090 Fragments arranged by size: 4090 613 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2064 2190 2908 1353 Size: 705 6 126 718 3423 Fragments arranged by size: 3423 718 705 126 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3565 3939 4085 387 Size: 1064 138 1976 374 146 1280 Fragments arranged by size: 1976 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2158 2215 2158 Size: 57 4921 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 3041 3638 4425 4693 1239 Size: 393 401 1008 597 787 268 1524 Fragments arranged by size: 1524 1008 787 597 401 393 268 EcoRI G'AATT_C Cuts at: 2287 2665 2287 Size: 378 4600 FspI TGC'GCA Cuts at: 3484 3484 Size: 4978 HaeII r_GCGC'y Cuts at: 1678 4125 1678 Size: 2447 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 3619 3651 3693 Size: 32 135 32 587 32 1428 32 42 Cuts at: 3693 3725 1373 Size: 32 2626 Fragments arranged by size: 2626 1428 587 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2322 2568 669 Size: 988 281 384 246 3079 Fragments arranged by size: 3079 988 384 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4978 HpaI GTT'AAC Cuts at: 2568 2568 Size: 4978 KpnI G_GTAC'C Cuts at: 2303 2303 Size: 4978 MfeI C'AATT_G Cuts at: 2577 2577 Size: 4978 MluI A'CGCG_T Cuts at: 4854 4854 Size: 4978 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2103 4092 4276 1305 Size: 798 1989 184 2007 Fragments arranged by size: 2007 1989 798 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3156 Fragments arranged by size: 3156 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2936 3295 3454 518 Size: 648 1770 359 159 2042 Fragments arranged by size: 2042 1770 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2394 3071 4219 4464 1606 Size: 788 677 1148 245 2120 Fragments arranged by size: 2120 1148 788 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3487 Fragments arranged by size: 3487 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4978 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4978 NotI GC'GGCC_GC Cuts at: 2425 2425 Size: 4978 NspI r_CATG'y Cuts at: 1132 1229 2234 2336 3881 1132 Size: 97 1005 102 1545 2229 Fragments arranged by size: 2229 1545 1005 102 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4978 PciI A'CATG_T Cuts at: 3877 3877 Size: 4978 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 1108 Size: 1140 3838 PmeI GTTT'AAAC Cuts at: 2282 2282 Size: 4978 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3047 3079 4560 4592 3047 Size: 32 1481 32 3433 Fragments arranged by size: 3433 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4978 PsiI TTA'TAA Cuts at: 2548 2548 Size: 4978 PstI C_TGCA'G Cuts at: 830 2330 830 Size: 1500 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4978 SacI G_AGCT'C Cuts at: 2160 2217 2160 Size: 57 4921 SalI G'TCGA_C Cuts at: 1936 2320 1936 Size: 384 4594 SbfI CC_TGCA'GG Cuts at: 2330 2330 Size: 4978 ScaI AGT'ACT Cuts at: 3226 4938 3226 Size: 1712 3266 SfcI C'TryA_G Cuts at: 826 1045 2326 3461 4142 4333 826 Size: 219 1281 1135 681 191 1471 Fragments arranged by size: 1471 1281 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4978 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4978 SmaI CCC'GGG Cuts at: 1872 2144 2305 1872 Size: 272 161 4545 Fragments arranged by size: 4545 272 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 3082 3983 4245 820 Size: 197 926 1139 901 262 1553 Fragments arranged by size: 1553 1139 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2769 493 Size: 2276 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4978 SphI G_CATG'C Cuts at: 1132 2234 2336 1132 Size: 1102 102 3774 Fragments arranged by size: 3774 1102 102 SspI AAT'ATT Cuts at: 5 52 2902 5 Size: 47 2850 2081 Fragments arranged by size: 2850 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4978 StyI C'CwwG_G Cuts at: 513 1065 2004 2417 513 Size: 552 939 413 3074 Fragments arranged by size: 3074 939 552 413 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3005 3022 3175 3360 3805 325 Size: 1255 1425 17 153 185 445 1498 Fragments arranged by size: 1498 1425 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3224 4936 96 Size: 274 80 33 51 1443 1247 1712 138 Fragments arranged by size: 1712 1443 1247 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4938 4970 4938 Size: 32 4946 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4978 XbaI T'CTAG_A Cuts at: 2314 2314 Size: 4978 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4978 XmaI C'CCGG_G Cuts at: 1870 2142 2303 1870 Size: 272 161 4545 Fragments arranged by size: 4545 272 161 XmnI GAAnn'nnTTC Cuts at: 3107 3107 Size: 4978 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2786 276 Size: 53 83 186 936 1252 2468 Fragments arranged by size: 2468 1252 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI