(Circular) (Six-base) MAPSORT of: pFN21AE6772.seq Check: 457 from: 1 to: 6544 pFN21AE6772 6544 bp With 182 enzymes: SgfI * July 24, 2018 19:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4354 278 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3887 1937 Size: 1950 4594 Acc65I G'GTAC_C Cuts at: 3865 3865 Size: 6544 AclI AA'CG_TT Cuts at: 1563 4671 5044 1563 Size: 3108 373 3063 Fragments arranged by size: 3108 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2514 4607 5991 6259 1632 Size: 708 174 2093 1384 268 1917 Fragments arranged by size: 2093 1917 1384 708 268 174 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6347 AflIII A'CryG_T Cuts at: 1203 5443 6420 1203 Size: 4240 977 1327 Fragments arranged by size: 4240 1327 977 AgeI A'CCGG_T Cuts at: 5375 5375 Size: 6544 AhdI GACnn_n'nnGTC Cuts at: 5273 5273 Size: 6544 AleI CACnn'nnGTG Cuts at: 1166 2990 1166 Size: 1824 4720 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6544 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2355 2387 2355 Size: 32 6512 AlwNI CAG_nnn'CTG Cuts at: 2050 2653 3054 3581 4287 5859 2050 Size: 603 401 527 706 1572 2735 Fragments arranged by size: 2735 1572 706 603 527 401 ApaI G_GGCC'C Cuts at: 3467 3467 Size: 6544 ApaLI G'TGCA_C Cuts at: 4601 5757 4601 Size: 1156 5388 ApoI r'AATT_y Cuts at: 1493 2298 3853 4079 4231 6340 1493 Size: 805 1555 226 152 2109 1697 Fragments arranged by size: 2109 1697 1555 805 226 152 AseI AT'TA_AT Cuts at: 160 1032 5098 160 Size: 872 4066 1606 Fragments arranged by size: 4066 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 AvaI C'yCGr_G Cuts at: 1870 1943 2086 2460 2973 3008 3869 1870 Size: 73 143 374 513 35 861 4545 Fragments arranged by size: 4545 861 513 374 143 73 35 BamHI G'GATC_C Cuts at: 2464 3874 4241 2464 Size: 1410 367 4767 Fragments arranged by size: 4767 1410 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 2246 2386 3865 5320 Size: 325 217 627 266 193 140 1479 1455 Cuts at: 5320 618 Size: 1842 Fragments arranged by size: 1842 1479 1455 627 325 266 217 193 140 BanII G_rGCy'C Cuts at: 1391 2461 3210 3467 3863 1391 Size: 1070 749 257 396 4072 Fragments arranged by size: 4072 1070 749 396 257 BbeI G_GCGC'C Cuts at: 2390 2390 Size: 6544 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3846 928 Size: 971 1947 3626 Fragments arranged by size: 3626 1947 971 BbvCI CC'TCA_GC Cuts at: 3047 3047 Size: 6544 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4735 4769 1903 Size: 34 2798 34 3678 Fragments arranged by size: 3678 2798 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4436 5646 3596 Size: 840 1210 4494 Fragments arranged by size: 4494 1210 840 BclI T'GATC_A Cuts at: 1548 3370 1548 Size: 1822 4722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3433 5155 136 Size: 107 122 71 631 605 1761 1722 1525 Fragments arranged by size: 1761 1722 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3802 6539 3802 Size: 2737 3807 BlpI GC'TnA_GC Cuts at: 3423 3961 3423 Size: 538 6006 Bme1580I G_kGCm'C Cuts at: 1790 2058 2987 3467 4605 5761 1790 Size: 268 929 480 1138 1156 2573 Fragments arranged by size: 2573 1156 1138 929 480 268 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2700 4275 5233 Size: 416 53 83 399 24 1283 1575 958 Cuts at: 5233 442 Size: 1753 Fragments arranged by size: 1753 1575 1283 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2172 1056 Size: 1116 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2844 3224 3420 3435 3472 5204 1239 Size: 893 712 380 196 15 37 1732 2579 Fragments arranged by size: 2579 1732 893 712 380 196 37 15 Bpu10I CC'TnA_GC Cuts at: 2502 2646 3047 2502 Size: 144 401 5999 Fragments arranged by size: 5999 401 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2361 4669 5534 5832 2361 Size: 2308 865 298 3073 Fragments arranged by size: 3073 2308 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5207 882 Size: 617 3708 2219 Fragments arranged by size: 3708 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4335 493 Size: 711 3131 2702 Fragments arranged by size: 3131 2702 711 BsaBI GATnn'nnATC Cuts at: 6538 6538 Size: 6544 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2387 4351 4733 5368 Size: 53 83 186 936 854 1964 382 635 Cuts at: 5368 275 Size: 1451 Fragments arranged by size: 1964 1451 936 854 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2010 4977 5375 5649 5796 6207 2010 Size: 2967 398 274 147 411 2347 Fragments arranged by size: 2967 2347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2355 2385 2817 2847 3619 Size: 30 165 30 952 30 432 30 772 Cuts at: 3619 3649 1178 Size: 30 4073 Fragments arranged by size: 4073 952 772 432 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2514 1186 Size: 1328 5216 BseYI C'CCAG_C Cuts at: 3638 5747 6243 3638 Size: 2109 496 3939 Fragments arranged by size: 3939 2109 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3525 1163 Size: 573 1789 4182 Fragments arranged by size: 4182 1789 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3994 4755 5783 1540 Size: 275 186 1993 761 1028 2301 Fragments arranged by size: 2301 1993 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2959 3366 4605 4690 5761 2959 Size: 407 1239 85 1071 3742 Fragments arranged by size: 3742 1239 1071 407 85 BsmI GAATG_Cn' Cuts at: 3762 4055 4148 3762 Size: 293 93 6158 Fragments arranged by size: 6158 293 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2461 2959 2987 3210 3366 3467 Size: 399 268 403 498 28 223 156 101 Cuts at: 3467 3863 4605 4690 5761 1391 Size: 396 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 498 403 399 396 268 223 156 101 85 28 BspEI T'CCGG_A Cuts at: 6207 6207 Size: 6544 BspHI T'CATG_A Cuts at: 3835 4433 6163 3835 Size: 598 1730 4216 Fragments arranged by size: 4216 1730 598 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2661 3899 844 Size: 1817 1238 3489 Fragments arranged by size: 3489 1817 1238 BsrBI CCG'CTC Cuts at: 1394 1823 4431 1394 Size: 429 2608 3507 Fragments arranged by size: 3507 2608 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5039 5213 65 Size: 1183 3791 174 1396 Fragments arranged by size: 3791 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 5188 5375 1700 Size: 110 290 3088 187 2869 Fragments arranged by size: 3088 2869 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6544 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6544 BssSI C'ACGA_G Cuts at: 4604 5616 4604 Size: 1012 5532 BstAPI GCAn_nnn'nTGC Cuts at: 794 3317 794 Size: 2523 4021 BstBI TT'CG_AA Cuts at: 5348 5348 Size: 6544 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6544 BstYI r'GATC_y Cuts at: 1972 2447 2464 2497 3030 3802 3874 4241 4626 Size: 475 17 33 533 772 72 367 385 Cuts at: 4626 4643 6095 6181 6193 6539 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 772 533 475 385 367 346 86 72 33 17 17 12 Bsu36I CC'TnA_GG Cuts at: 3707 3707 Size: 6544 BtgI C'CryG_G Cuts at: 513 1065 3060 513 Size: 552 1995 3997 Fragments arranged by size: 3997 1995 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2140 487 Size: 683 56 914 4891 Fragments arranged by size: 4891 914 683 56 BtsI GCAGTG_nn' Cuts at: 2573 3136 4060 4854 4874 2573 Size: 563 924 794 20 4243 Fragments arranged by size: 4243 924 794 563 20 ClaI AT'CG_AT Cuts at: 1554 2196 1554 Size: 642 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3214 3249 529 Size: 35 2650 35 3824 Fragments arranged by size: 3824 2650 35 35 DraI TTT'AAA Cuts at: 3848 3848 Size: 6544 DraIII CAC_nnn'GTG Cuts at: 3187 3187 Size: 6544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2561 3719 5551 809 Size: 507 1245 1158 1832 1802 Fragments arranged by size: 1832 1802 1245 1158 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3222 3991 4880 Size: 54 1425 50 275 18 1392 769 889 Cuts at: 4880 6509 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1392 889 769 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3991 1537 Size: 275 2179 4090 Fragments arranged by size: 4090 2179 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2831 3008 4474 1353 Size: 1478 177 1466 3423 Fragments arranged by size: 3423 1478 1466 177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5131 5505 5651 387 Size: 1064 138 3542 374 146 1280 Fragments arranged by size: 3542 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2340 2514 2844 3224 3420 3435 Size: 393 500 208 174 330 380 196 15 Cuts at: 3435 3472 4607 5204 5991 6259 1239 Size: 37 1135 597 787 268 1524 Fragments arranged by size: 1524 1135 787 597 500 393 380 330 268 208 196 174 37 15 EcoO109I rG'GnC_Cy Cuts at: 2650 3464 2650 Size: 814 5730 EcoRI G'AATT_C Cuts at: 3853 4231 3853 Size: 378 6166 FspI TGC'GCA Cuts at: 5050 5050 Size: 6544 HaeII r_GCGC'y Cuts at: 1678 2390 5691 1678 Size: 712 3301 2531 Fragments arranged by size: 3301 2531 712 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3593 3625 5185 5217 5259 Size: 32 135 32 2021 32 1560 32 42 Cuts at: 5259 5291 1373 Size: 32 2626 Fragments arranged by size: 2626 2021 1560 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3888 4134 669 Size: 988 281 1950 246 3079 Fragments arranged by size: 3079 1950 988 281 246 HindIII A'AGCT_T Cuts at: 748 2878 748 Size: 2130 4414 HpaI GTT'AAC Cuts at: 4134 4134 Size: 6544 KasI G'GCGC_C Cuts at: 2386 2386 Size: 6544 KpnI G_GTAC'C Cuts at: 3869 3869 Size: 6544 MfeI C'AATT_G Cuts at: 4143 4143 Size: 6544 MluI A'CGCG_T Cuts at: 6420 6420 Size: 6544 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3100 5658 5842 1305 Size: 1795 2558 184 2007 Fragments arranged by size: 2558 2007 1795 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3224 10 Size: 54 1425 343 1392 3330 Fragments arranged by size: 3330 1425 1392 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2990 3305 4502 4861 5020 518 Size: 648 1824 315 1197 359 159 2042 Fragments arranged by size: 2042 1824 1197 648 359 315 159 MspA1I CmG'CkG Cuts at: 1606 2429 2710 3194 3960 4637 5785 6030 1606 Size: 823 281 484 766 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 766 677 484 281 245 NarI GG'CG_CC Cuts at: 2387 2387 Size: 6544 NcoI C'CATG_G Cuts at: 513 1065 3060 513 Size: 552 1995 3997 Fragments arranged by size: 3997 1995 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6544 NheI G'CTAG_C Cuts at: 1052 2168 1052 Size: 1116 5428 NotI GC'GGCC_GC Cuts at: 3991 3991 Size: 6544 NspI r_CATG'y Cuts at: 1132 1229 2135 2518 3327 3902 5447 1132 Size: 97 906 383 809 575 1545 2229 Fragments arranged by size: 2229 1545 906 809 575 383 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6544 PciI A'CATG_T Cuts at: 5443 5443 Size: 6544 PflMI CCAn_nnn'nTGG Cuts at: 1108 3187 1108 Size: 2079 4465 PmeI GTTT'AAAC Cuts at: 3848 3848 Size: 6544 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2355 2387 4613 4645 6126 6158 2355 Size: 32 2226 32 1481 32 2741 Fragments arranged by size: 2741 2226 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2650 2650 Size: 6544 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6544 PsiI TTA'TAA Cuts at: 4114 4114 Size: 6544 PspOMI G'GGCC_C Cuts at: 3463 3463 Size: 6544 PspXI vC'TCGA_Gb Cuts at: 2973 2973 Size: 6544 PstI C_TGCA'G Cuts at: 830 2443 3896 830 Size: 1613 1453 3478 Fragments arranged by size: 3478 1613 1453 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6544 PvuII CAG'CTG Cuts at: 2429 2710 3194 2429 Size: 281 484 5779 Fragments arranged by size: 5779 484 281 RsrII CG'GwC_CG Cuts at: 2067 2067 Size: 6544 SalI G'TCGA_C Cuts at: 1936 3886 1936 Size: 1950 4594 SbfI CC_TGCA'GG Cuts at: 3896 3896 Size: 6544 ScaI AGT'ACT Cuts at: 4792 6504 4792 Size: 1712 4832 SfcI C'TryA_G Cuts at: 826 1045 2439 3892 5027 5708 5899 826 Size: 219 1394 1453 1135 681 191 1471 Fragments arranged by size: 1471 1453 1394 1135 681 219 191 SfoI GGC'GCC Cuts at: 2388 2388 Size: 6544 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6544 SmaI CCC'GGG Cuts at: 1872 3871 1872 Size: 1999 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2376 2973 4648 5549 5811 820 Size: 197 926 433 597 1675 901 262 1553 Fragments arranged by size: 1675 1553 926 901 597 433 262 197 SnaBI TAC'GTA Cuts at: 493 4335 493 Size: 3842 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6544 SphI G_CATG'C Cuts at: 1132 3327 3902 1132 Size: 2195 575 3774 Fragments arranged by size: 3774 2195 575 SspI AAT'ATT Cuts at: 5 52 4468 5 Size: 47 4416 2081 Fragments arranged by size: 4416 2081 47 StuI AGG'CCT Cuts at: 1517 2022 3617 1517 Size: 505 1595 4444 Fragments arranged by size: 4444 1595 505 StyI C'CwwG_G Cuts at: 513 1065 2058 2935 2979 3060 3983 513 Size: 552 993 877 44 81 923 3074 Fragments arranged by size: 3074 993 923 877 552 81 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3295 4571 4588 4741 4926 5371 325 Size: 1255 1715 1276 17 153 185 445 1498 Fragments arranged by size: 1715 1498 1276 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2704 4790 6502 Size: 274 80 33 51 1443 727 2086 1712 Cuts at: 6502 96 Size: 138 Fragments arranged by size: 2086 1712 1443 727 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2817 2849 6504 6536 2817 Size: 32 3655 32 2825 Fragments arranged by size: 3655 2825 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3607 1316 Size: 2291 4253 XbaI T'CTAG_A Cuts at: 2140 3880 2140 Size: 1740 4804 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3217 3217 Size: 6544 XhoI C'TCGA_G Cuts at: 1943 2973 1943 Size: 1030 5514 XmaI C'CCGG_G Cuts at: 1870 3869 1870 Size: 1999 4545 XmnI GAAnn'nnTTC Cuts at: 2994 4673 2994 Size: 1679 4865 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4352 276 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI SacI SacII SanDI SapI SexAI SfiI SrfI SwaI