################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII AclI AcsI AflII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AviII AvrII BciVI BfrI BlnI BsaBI BsiWI BsmBI Bsp1407I BspDI BsrGI BssSI Bst1107I BstBI BstZ17I ClaI DraI DraIII Eco47III EcoNI EcoRI EcoRV FseI FspI HindIII MamI MluI NdeI NheI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 488 GGTACC G^GTACC AccI 3142 GTCGAC GT^[AC][GT]AC AccIII 3636 TCCGGA T^CCGGA AflIII 2336 ACATGT A^C[GA][CT]GT AhdI 193 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 488 GGTACC G^GTACC AspEI 193 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcgI 1031 GCATCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 440 TGATCA T^GATCA BglII 2928 AGATCT A^GATCT BsaWI 3636 TCCGGA [AT]^CCGG[AT] BseAI 3636 TCCGGA T^CCGGA BsmI 1996 GAATGC GAATGC(1/-1) BspEI 3636 TCCGGA T^CCGGA BspHI 2634 TCATGA T^CATGA BspLU11I 2336 ACATGT A^CATGT BssHII 629 GCGCGC G^CGCGC BstAPI 3580 GCAAACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 87 GGTGACC G^GT[ACGT]ACC BstPI 87 GGTGACC G^GT[ACGT]ACC CpoI 906 CGGTCCG CG^G[AT]CCG Eam1105I 193 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 87 GGTGACC G^GT[ACGT]ACC EcoT22I 958 ATGCAT ATGCA^T FbaI 440 TGATCA T^GATCA HpaI 1053 GTTAAC GTT^AAC KpnI 488 GGTACC GGTAC^C MfeI 1473 CAATTG C^AATTG MroI 3636 TCCGGA T^CCGGA MunI 1473 CAATTG C^AATTG NotI 391 GCGGCCGC GC^GGCCGC NsiI 958 ATGCAT ATGCA^T RcaI 2634 TCATGA T^CATGA RsrII 906 CGGTCCG CG^G[AT]CCG SalI 3142 GTCGAC G^TCGAC SmaI 3429 CCCGGG CCC^GGG XbaI 1636 TCTAGA T^CTAGA XmaCI 3429 CCCGGG C^CCGGG XmaI 3429 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 832 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 3452 CAGCTGCTG AocI 1146 CCTCAGG CC^T[ACGT]AGG 2386 CCTCAGG AspI 135 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2758 GACTACGTC BamHI 1582 GGATCC G^GATCC 1795 GGATCC BbeI 403 GGCGCC GGCGC^C 1042 GGCGCC BbrPI 2948 CACGTG CAC^GTG 3712 CACGTG BbvCI 3344 CCTCAGC CCTCAGC(-5/-2) 3387 CCTCAGC Bsu36I 1146 CCTCAGG CC^T[ACGT]AGG 2386 CCTCAGG EagI 392 CGGCCG C^GGCCG 650 CGGCCG EclXI 392 CGGCCG C^GGCCG 650 CGGCCG Eco52I 392 CGGCCG C^GGCCG 650 CGGCCG Eco81I 1146 CCTCAGG CC^T[ACGT]AGG 2386 CCTCAGG HgaI 310 GACGC GACGC(5/10) 3248 GACGC KasI 403 GGCGCC G^GCGCC 1042 GGCGCC KspI 371 CCGCGG CCGC^GG 430 CCGCGG NaeI 648 GCCGGC GCC^GGC 1284 GCCGGC NarI 403 GGCGCC GG^CGCC 1042 GGCGCC NcoI 1737 CCATGG C^CATGG 3083 CCATGG NgoMIV 648 GCCGGC G^CCGGC 1284 GCCGGC NruI 21 TCGCGA TCG^CGA 530 TCGCGA PflFI 135 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2758 GACTACGTC PflMI 1912 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2405 CCAAAAGTTGG PmaCI 2948 CACGTG CAC^GTG 3712 CACGTG PmlI 2948 CACGTG CAC^GTG 3712 CACGTG SacII 371 CCGCGG CCGC^GG 430 CCGCGG SapI 3232 GAAGAGC GCTCTTC(1/4) 2396 GCTCTTC SfoI 403 GGCGCC GGC^GCC 1042 GGCGCC Tth111I 135 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2758 GACTACGTC Van91I 1912 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2405 CCAAAAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII