(Circular) (Six-base) MAPSORT of: pFN21AE5666.seq Check: 4458 from: 1 to: 6265 pFN21AE5666 6265 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2284 3024 4328 5712 5980 1632 Size: 652 740 1304 1384 268 1917 Fragments arranged by size: 1917 1384 1304 740 652 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 2982 5164 6141 1203 Size: 1779 2182 977 1327 Fragments arranged by size: 2182 1779 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 2867 1166 Size: 1701 4564 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 3410 4008 5580 3410 Size: 598 1572 4095 Fragments arranged by size: 4095 1572 598 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 2630 3574 3800 3952 6061 1493 Size: 1137 944 226 152 2109 1697 Fragments arranged by size: 2109 1697 1137 944 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2109 3345 3590 1870 Size: 73 166 1236 245 4545 Fragments arranged by size: 4545 1236 245 166 73 BamHI G'GATC_C Cuts at: 2177 3219 3595 3962 2177 Size: 1042 376 367 4480 Fragments arranged by size: 4480 1042 376 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3278 3586 5041 618 Size: 325 217 627 1491 308 1455 1842 Fragments arranged by size: 1842 1491 1455 627 325 308 217 BanII G_rGCy'C Cuts at: 1391 3584 1391 Size: 2193 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4456 4490 1903 Size: 34 2519 34 3678 Fragments arranged by size: 3678 2519 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 2859 1548 Size: 1311 4954 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4876 136 Size: 107 122 71 631 605 3204 1525 Fragments arranged by size: 3204 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 4326 5482 1790 Size: 2536 1156 2573 Fragments arranged by size: 2573 2536 1156 BmgBI CAC'GTC Cuts at: 1318 2023 3065 1318 Size: 705 1042 4518 Fragments arranged by size: 4518 1042 705 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3996 4954 442 Size: 416 53 83 399 24 2579 958 1753 Fragments arranged by size: 2579 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3199 3199 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2717 2941 2955 3158 3319 4925 1239 Size: 1478 224 14 203 161 1606 2579 Fragments arranged by size: 2579 1606 1478 224 203 161 14 Bpu10I CC'TnA_GC Cuts at: 3482 3482 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2230 4390 5255 5553 2230 Size: 2160 865 298 2942 Fragments arranged by size: 2942 2160 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2231 2525 2548 2676 2741 4928 882 Size: 617 732 294 23 128 65 2187 2219 Fragments arranged by size: 2219 2187 732 617 294 128 65 23 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4072 4454 5089 275 Size: 53 83 186 936 2539 382 635 1451 Fragments arranged by size: 2539 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2673 2703 1178 Size: 30 165 30 1270 30 4740 Fragments arranged by size: 4740 1270 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3188 1186 Size: 2002 4263 BseYI C'CCAG_C Cuts at: 5468 5964 5468 Size: 496 5769 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3408 1163 Size: 573 1672 4020 Fragments arranged by size: 4020 1672 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3715 4476 5504 1540 Size: 275 186 1714 761 1028 2301 Fragments arranged by size: 2301 1714 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4326 4411 5482 4326 Size: 85 1071 5109 Fragments arranged by size: 5109 1071 85 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3584 4326 4411 5482 1391 Size: 399 1794 742 85 1071 2174 Fragments arranged by size: 2174 1794 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 4154 5884 4154 Size: 1730 4535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3620 844 Size: 2776 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2662 3303 4152 1394 Size: 429 839 641 849 3507 Fragments arranged by size: 3507 849 839 641 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4909 5096 1700 Size: 110 3099 187 2869 Fragments arranged by size: 3099 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 2865 4325 5337 2865 Size: 1460 1012 3793 Fragments arranged by size: 3793 1460 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2016 794 Size: 1222 5043 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstXI CCAn_nnnn'nTGG Cuts at: 2322 3058 3118 3448 2322 Size: 736 60 330 5139 Fragments arranged by size: 5139 736 330 60 BstYI r'GATC_y Cuts at: 1972 2177 2642 3219 3351 3595 3962 4347 4364 Size: 205 465 577 132 244 367 385 17 Cuts at: 4364 5816 5902 5914 6260 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 577 465 385 367 346 244 205 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2405 3363 513 Size: 552 1340 958 3415 Fragments arranged by size: 3415 1340 958 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 2523 2582 3781 4575 4595 2523 Size: 59 1199 794 20 4193 Fragments arranged by size: 4193 1199 794 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2488 2523 529 Size: 35 1924 35 4271 Fragments arranged by size: 4271 1924 35 35 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3712 4601 6230 Size: 54 1425 50 275 18 1882 889 1629 Cuts at: 6230 8 Size: 43 Fragments arranged by size: 1882 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3712 1537 Size: 275 1900 4090 Fragments arranged by size: 4090 1900 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2421 4195 1353 Size: 1068 1774 3423 Fragments arranged by size: 3423 1774 1068 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2655 4852 5226 5372 387 Size: 1064 138 1066 2197 374 146 1280 Fragments arranged by size: 2197 1280 1066 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2284 2717 2941 2955 3024 3158 3319 Size: 393 652 433 224 14 69 134 161 Cuts at: 3319 4328 4925 5712 5980 1239 Size: 1009 597 787 268 1524 Fragments arranged by size: 1524 1009 787 652 597 433 393 268 224 161 134 69 14 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2839 2839 Size: 6265 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 5412 1678 Size: 3734 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2122 2154 2721 2753 2848 Size: 32 135 32 550 32 567 32 95 Cuts at: 2848 2880 3211 3243 4906 4938 4980 5012 1373 Size: 32 331 32 1663 32 42 32 2626 Fragments arranged by size: 2626 1663 567 550 331 135 95 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2167 2967 5379 5563 1305 Size: 862 800 2412 184 2007 Fragments arranged by size: 2412 2007 862 800 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4443 Fragments arranged by size: 4443 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2320 2444 2867 4223 4582 4741 518 Size: 648 1154 124 423 1356 359 159 2042 Fragments arranged by size: 2042 1356 1154 648 423 359 159 124 MspA1I CmG'CkG Cuts at: 1606 3681 4358 5506 5751 1606 Size: 2075 677 1148 245 2120 Fragments arranged by size: 2120 2075 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2405 3363 513 Size: 552 1340 958 3415 Fragments arranged by size: 3415 1340 958 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 3362 3623 5168 1132 Size: 97 2133 261 1545 2229 Fragments arranged by size: 2229 2133 1545 261 97 PasI CC'CwG_GG Cuts at: 1348 3215 1348 Size: 1867 4398 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 3256 3297 1108 Size: 2148 41 4076 Fragments arranged by size: 4076 2148 41 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 2907 3835 2907 Size: 928 5337 PstI C_TGCA'G Cuts at: 830 2530 3617 830 Size: 1700 1087 3478 Fragments arranged by size: 3478 1700 1087 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 3182 4513 6225 3182 Size: 1331 1712 3222 Fragments arranged by size: 3222 1712 1331 SfcI C'TryA_G Cuts at: 826 1045 2526 3321 3613 4748 5429 5620 826 Size: 219 1481 795 292 1135 681 191 1471 Fragments arranged by size: 1481 1471 1135 795 681 292 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 2111 3592 1872 Size: 239 1481 4545 Fragments arranged by size: 4545 1481 239 SmlI C'TyrA_G Cuts at: 820 1017 1943 2209 4369 5270 5532 820 Size: 197 926 266 2160 901 262 1553 Fragments arranged by size: 2160 1553 926 901 266 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3362 3623 1132 Size: 2230 261 3774 Fragments arranged by size: 3774 2230 261 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 2704 3477 1517 Size: 1187 773 4305 Fragments arranged by size: 4305 1187 773 StyI C'CwwG_G Cuts at: 513 1065 2170 2220 2405 2677 3111 3363 3704 Size: 552 1105 50 185 272 434 252 341 Cuts at: 3704 513 Size: 3074 Fragments arranged by size: 3074 1105 552 434 341 272 252 185 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2700 4292 4309 4462 4647 5092 325 Size: 1255 1120 1592 17 153 185 445 1498 Fragments arranged by size: 1592 1498 1255 1120 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3180 4511 6223 Size: 274 80 33 51 1443 1203 1331 1712 Cuts at: 6223 96 Size: 138 Fragments arranged by size: 1712 1443 1331 1203 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 2673 2705 6225 6257 2076 Size: 32 565 32 3520 32 2084 Fragments arranged by size: 3520 2084 565 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2024 1316 Size: 708 5557 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3445 3445 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 2109 3590 1870 Size: 239 1481 4545 Fragments arranged by size: 4545 1481 239 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI