################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccI AccII AccIII AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BfaI BfrI BlnI BsaAI BsaHI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tru9I Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 299 GGTACC G^GTACC AclI 217 AACGTT AA^CGTT AlwNI 909 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 990 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 299 GGTACC G^GTACC AspHI 278 GTGCTC G[AT]GC[AT]^C AvaI 108 CCCGGG C^[CT]CG[GA]G BamHI 113 GGATCC G^GATCC BanI 299 GGTACC G^G[CT][GA]CC BlpI 972 GCTGAGC GC^T[ACGT]AGC Bpu1102I 972 GCTGAGC GC^T[ACGT]AGC BsaWI 1037 TCCGGT [AT]^CCGG[AT] BseRI 262 CTCCTC GAGGAG(10/8) BsiHKAI 278 GTGCTC G[AT]GC[AT]^C BsmFI 102 GTCCC GGGAC(10/14) BsoBI 108 CCCGGG C^[CT]CG[GA]G Bsp1407I 196 TGTACA T^GTACA BspLU11I 750 ACATGT A^CATGT BsrDI 696 CATTGC GCAATG(2/0) BsrGI 196 TGTACA T^GTACA BssSI 1138 CACGAG CACGAG(-5/-1) BstXI 503 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 972 GCTGAGC GC^T[ACGT]AGC CfoI 397 GCGC GCG^C EcoRV 439 GATATC GAT^ATC FauI 920 CCCGC CCCGC(4/6) HaeII 396 GGCGCT [GA]GCGC^[CT] HhaI 397 GCGC GCG^C HinP1I 397 GCGC G^CGC HinfI 769 GAATC G^A[ACGT]TC KpnI 299 GGTACC GGTAC^C MspA1I 1006 CAGCTG C[AC]G^C[GT]G NspI 750 ACATGT [GA]CATG^[CT] PflMI 627 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 217 AACGTT AA^CGTT PstI 131 CTGCAG CTGCA^G PvuII 1006 CAGCTG CAG^CTG SfcI 131 CTGCAG C^T[GA][CT]AG SmaI 108 CCCGGG CCC^GGG SmlI 1081 CTCAAG C^T[CT][GA]AG SspBI 196 TGTACA T^GTACA TaqI 670 TCGA T^CGA TfiI 769 GAATC G^A[AT]TC TthHB8I 670 TCGA T^CGA Van91I 627 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 108 CCCGGG C^CCGGG XmaI 108 CCCGGG C^CCGGG XmnI 990 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 274 ACGTGT A^C[GA][CT]GT 750 ACATGT AvaII 39 GGACC G^G[AT]CC 101 GGTCC BalI 512 TGGCCA TGG^CCA 1103 TGGCCA BbsI 382 GTCTTC GAAGAC(2/6) 649 GTCTTC BclI 182 TGATCA T^GATCA 1031 TGATCA BglI 161 GCCTCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1019 GCCAGCCTGGC BglII 188 AGATCT A^GATCT 467 AGATCT BpmI 63 CTCCAG CTGGAG(16/14) 281 CTCCAG BpuAI 382 GTCTTC GAAGAC(2/6) 649 GTCTTC BsaBI 183 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 462 GATGAAGATC BsaI 254 GAGACC GGTCTC(1/5) 1040 GGTCTC BsmAI 254 GAGAC GTCTC(1/5) 1041 GTCTC BsrBI 858 CCGCTC CCGCTC(-3/-3) 1141 GAGCGG DsaI 734 CCGTGG C^C[GA][CT]GG 1100 CCGTGG FbaI 182 TGATCA T^GATCA 1031 TGATCA MamI 183 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 462 GATGAAGATC MluNI 512 TGGCCA TGG^CCA 1103 TGGCCA MscI 512 TGGCCA TGG^CCA 1103 TGGCCA SfaNI 289 GATGC GCATC(5/9) 911 GCATC StuI 159 AGGCCT AGG^CCT 446 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI XcmI XhoII