(Circular) (Six-base) MAPSORT of: pFN21AB6508.seq Check: 4769 from: 1 to: 5857 pFN21AB6508 5857 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3200 1937 Size: 1263 4594 Acc65I G'GTAC_C Cuts at: 2304 3178 2304 Size: 874 4983 AclI AA'CG_TT Cuts at: 1563 2223 3984 4357 1563 Size: 660 1761 373 3063 Fragments arranged by size: 3063 1761 660 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3920 5304 5572 1632 Size: 2288 1384 268 1917 Fragments arranged by size: 2288 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 2279 2755 4756 5733 1203 Size: 1076 476 2001 977 1327 Fragments arranged by size: 2001 1327 1076 977 476 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5857 AlwNI CAG_nnn'CTG Cuts at: 2919 3600 5172 2919 Size: 681 1572 3604 Fragments arranged by size: 3604 1572 681 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 3166 3392 3544 5653 1493 Size: 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2113 3182 1870 Size: 73 170 1069 4545 Fragments arranged by size: 4545 1069 170 73 BamHI G'GATC_C Cuts at: 2118 3187 3554 2118 Size: 1069 367 4421 Fragments arranged by size: 4421 1069 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2304 3178 4633 618 Size: 325 217 627 517 874 1455 1842 Fragments arranged by size: 1842 1455 874 627 517 325 217 BanII G_rGCy'C Cuts at: 1391 3176 1391 Size: 1785 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2380 2647 928 Size: 971 481 267 4138 Fragments arranged by size: 4138 971 481 267 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 2187 3036 1548 Size: 639 849 4369 Fragments arranged by size: 4369 849 639 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2172 3030 4468 Size: 107 122 71 631 605 500 858 1438 Cuts at: 4468 136 Size: 1525 Fragments arranged by size: 1525 1438 858 631 605 500 122 107 71 BglII A'GATC_T Cuts at: 2193 2472 5852 2193 Size: 279 3380 2198 Fragments arranged by size: 3380 2198 279 BlpI GC'TnA_GC Cuts at: 2978 3274 2978 Size: 296 5561 Bme1580I G_kGCm'C Cuts at: 1790 2596 2926 3918 5074 1790 Size: 806 330 992 1156 2573 Fragments arranged by size: 2573 1156 992 806 330 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3588 4546 442 Size: 416 53 83 399 24 2171 958 1753 Fragments arranged by size: 2171 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2282 2282 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2053 2271 4517 1239 Size: 814 218 2246 2579 Fragments arranged by size: 2579 2246 814 218 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3071 3982 4847 5145 3071 Size: 911 865 298 3783 Fragments arranged by size: 3783 911 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2253 3051 4520 882 Size: 617 754 798 1469 2219 Fragments arranged by size: 2219 1469 798 754 617 BsaAI yAC'GTr Cuts at: 493 1204 3648 493 Size: 711 2444 2702 Fragments arranged by size: 2702 2444 711 BsaBI GATnn'nnATC Cuts at: 2192 2471 5851 2192 Size: 279 3380 2198 Fragments arranged by size: 3380 2198 279 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3664 4046 4681 275 Size: 53 83 186 936 2131 382 635 1451 Fragments arranged by size: 2131 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3042 4290 4688 4962 5109 5520 3042 Size: 1248 398 274 147 411 3379 Fragments arranged by size: 3379 1248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2269 2299 1178 Size: 30 165 30 866 30 4736 Fragments arranged by size: 4736 866 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 1186 Size: 1072 4785 BseYI C'CCAG_C Cuts at: 5060 5556 5060 Size: 496 5361 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5284 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3307 4068 5096 1540 Size: 275 186 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2287 3918 4003 5074 2287 Size: 1631 85 1071 3070 Fragments arranged by size: 3070 1631 1071 85 BsmI GAATG_Cn' Cuts at: 3368 3461 3368 Size: 93 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2287 2596 2926 3176 3918 4003 5074 Size: 399 497 309 330 250 742 85 1071 Cuts at: 5074 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 497 399 330 309 250 85 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 2292 2982 3080 3212 844 Size: 1427 21 690 98 132 3489 Fragments arranged by size: 3489 1427 690 132 98 21 BsrBI CCG'CTC Cuts at: 1394 1823 2865 3148 3744 1394 Size: 429 1042 283 596 3507 Fragments arranged by size: 3507 1042 596 429 283 BsrDI GCAATG_nn' Cuts at: 65 1248 2700 4352 4526 65 Size: 1183 1452 1652 174 1396 Fragments arranged by size: 1652 1452 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 2201 96 Size: 2105 3752 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3143 3917 4929 3143 Size: 774 1012 4071 Fragments arranged by size: 4071 1012 774 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5857 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 5857 BstYI r'GATC_y Cuts at: 1972 2083 2118 2193 2472 3187 3554 3939 3956 Size: 111 35 75 279 715 367 385 17 Cuts at: 3956 5408 5494 5506 5852 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 715 385 367 346 279 111 86 75 35 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2739 3105 513 Size: 552 939 735 366 3265 Fragments arranged by size: 3265 939 735 552 366 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 2056 3373 4167 4187 2056 Size: 1317 794 20 3726 Fragments arranged by size: 3726 1317 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2435 2517 3108 Size: 54 1425 50 275 18 605 82 591 Cuts at: 3108 3304 4193 5822 8 Size: 196 889 1629 43 Fragments arranged by size: 1629 1425 889 605 591 275 196 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3304 1537 Size: 275 1492 4090 Fragments arranged by size: 4090 1492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3787 1353 Size: 2434 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4444 4818 4964 387 Size: 1064 138 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2271 3920 4517 5304 5572 1239 Size: 393 421 218 1649 597 787 268 1524 Fragments arranged by size: 1649 1524 787 597 421 393 268 218 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 EcoRV GAT'ATC Cuts at: 2446 2446 Size: 5857 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 2405 5004 1678 Size: 727 2599 2531 Fragments arranged by size: 2599 2531 727 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 4498 4530 4572 Size: 32 135 32 518 32 2376 32 42 Cuts at: 4572 4604 1373 Size: 32 2626 Fragments arranged by size: 2626 2376 518 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3201 3447 669 Size: 988 281 1263 246 3079 Fragments arranged by size: 3079 1263 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 2308 3182 2308 Size: 874 4983 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4971 5155 1305 Size: 3666 184 2007 Fragments arranged by size: 3666 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2519 3110 10 Size: 54 1425 343 687 591 2757 Fragments arranged by size: 2757 1425 687 591 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2507 2610 2894 3815 4174 4333 518 Size: 648 1341 103 284 921 359 159 2042 Fragments arranged by size: 2042 1341 921 648 359 284 159 103 MspA1I CmG'CkG Cuts at: 1606 3013 3273 3950 5098 5343 1606 Size: 1407 260 677 1148 245 2120 Fragments arranged by size: 2120 1407 1148 677 260 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4366 Fragments arranged by size: 4366 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 2759 3215 4760 1132 Size: 97 1530 456 1545 2229 Fragments arranged by size: 2229 1545 1530 456 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 2755 4756 2755 Size: 2001 3856 PflMI CCAn_nnn'nTGG Cuts at: 1108 2638 1108 Size: 1530 4327 PfoI T'CCnGG_A Cuts at: 2777 2777 Size: 5857 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PstI C_TGCA'G Cuts at: 830 2140 3209 830 Size: 1310 1069 3478 Fragments arranged by size: 3478 1310 1069 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 3013 3013 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SfcI C'TryA_G Cuts at: 826 1045 2136 3205 4340 5021 5212 826 Size: 219 1091 1069 1135 681 191 1471 Fragments arranged by size: 1471 1135 1091 1069 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 2115 3184 1872 Size: 243 1069 4545 Fragments arranged by size: 4545 1069 243 SmlI C'TyrA_G Cuts at: 820 1017 1943 3086 3961 4862 5124 820 Size: 197 926 1143 875 901 262 1553 Fragments arranged by size: 1553 1143 926 901 875 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 2166 2453 1517 Size: 649 287 4921 Fragments arranged by size: 4921 649 287 StyI C'CwwG_G Cuts at: 513 1065 2004 3296 513 Size: 552 939 1292 3074 Fragments arranged by size: 3074 1292 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3884 3901 4054 4239 4684 325 Size: 1255 2304 17 153 185 445 1498 Fragments arranged by size: 2304 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2201 3065 4103 Size: 274 80 33 51 1443 224 864 1038 Cuts at: 4103 5815 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1038 864 274 224 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5817 5849 5817 Size: 32 5825 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2251 2305 2800 3103 2251 Size: 54 495 303 5005 Fragments arranged by size: 5005 495 303 54 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 2113 3182 1870 Size: 243 1069 4545 Fragments arranged by size: 4545 1069 243 XmnI GAAnn'nnTTC Cuts at: 2999 3986 2999 Size: 987 4870 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI