(Circular) (Six-base) MAPSORT of: pFN21AE2370.seq Check: 769 from: 1 to: 5224 pFN21AE2370 5224 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3034 278 Size: 53 83 186 936 1498 2468 Fragments arranged by size: 2468 1498 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2567 1937 Size: 630 4594 Acc65I G'GTAC_C Cuts at: 2545 2545 Size: 5224 AclI AA'CG_TT Cuts at: 1563 3351 3724 1563 Size: 1788 373 3063 Fragments arranged by size: 3063 1788 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3287 4671 4939 1632 Size: 1655 1384 268 1917 Fragments arranged by size: 1917 1655 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5027 AflIII A'CryG_T Cuts at: 1203 4123 5100 1203 Size: 2920 977 1327 Fragments arranged by size: 2920 1327 977 AgeI A'CCGG_T Cuts at: 4055 4055 Size: 5224 AhdI GACnn_n'nnGTC Cuts at: 3953 3953 Size: 5224 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5224 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5224 AlwNI CAG_nnn'CTG Cuts at: 2967 4539 2967 Size: 1572 3652 ApaI G_GGCC'C Cuts at: 2052 2052 Size: 5224 ApaLI G'TGCA_C Cuts at: 3281 4437 3281 Size: 1156 4068 ApoI r'AATT_y Cuts at: 1493 2533 2759 2911 5020 1493 Size: 1040 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 226 152 AseI AT'TA_AT Cuts at: 160 1032 3778 160 Size: 872 2746 1606 Fragments arranged by size: 2746 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5224 AvaI C'yCGr_G Cuts at: 1870 1943 2549 1870 Size: 73 606 4545 Fragments arranged by size: 4545 606 73 BamHI G'GATC_C Cuts at: 2554 2921 2554 Size: 367 4857 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2545 4000 618 Size: 325 217 627 758 1455 1842 Fragments arranged by size: 1842 1455 758 627 325 217 BanII G_rGCy'C Cuts at: 1391 2052 2543 1391 Size: 661 491 4072 Fragments arranged by size: 4072 661 491 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4253 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3415 3449 1903 Size: 34 1478 34 3678 Fragments arranged by size: 3678 1478 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3116 4326 3116 Size: 1210 4014 BclI T'GATC_A Cuts at: 1548 1548 Size: 5224 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3835 136 Size: 107 122 71 631 605 2163 1525 Fragments arranged by size: 2163 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5219 5219 Size: 5224 BlpI GC'TnA_GC Cuts at: 2641 2641 Size: 5224 Bme1580I G_kGCm'C Cuts at: 1790 2052 3285 4441 1790 Size: 262 1233 1156 2573 Fragments arranged by size: 2573 1233 1156 262 BmgBI CAC'GTC Cuts at: 1318 2011 1318 Size: 693 4531 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2955 3913 442 Size: 416 53 83 399 24 1538 958 1753 Fragments arranged by size: 1753 1538 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5224 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3884 1239 Size: 2645 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3349 4214 4512 3349 Size: 865 298 4061 Fragments arranged by size: 4061 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3887 882 Size: 617 2388 2219 Fragments arranged by size: 2388 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3015 493 Size: 711 1811 2702 Fragments arranged by size: 2702 1811 711 BsaBI GATnn'nnATC Cuts at: 5218 5218 Size: 5224 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3031 3413 4048 275 Size: 53 83 186 936 1498 382 635 1451 Fragments arranged by size: 1498 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3657 4055 4329 4476 4887 3657 Size: 398 274 147 411 3994 Fragments arranged by size: 3994 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2497 2527 1178 Size: 30 165 30 1094 30 3875 Fragments arranged by size: 3875 1094 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2510 1186 Size: 1324 3900 BseYI C'CCAG_C Cuts at: 2060 4427 4923 2060 Size: 2367 496 2361 Fragments arranged by size: 2367 2361 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2291 1163 Size: 573 555 4096 Fragments arranged by size: 4096 573 555 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2206 2674 3435 4463 1540 Size: 275 186 205 468 761 1028 2301 Fragments arranged by size: 2301 1028 761 468 275 205 186 BsiHKAI G_wGCw'C Cuts at: 3285 3370 4441 3285 Size: 85 1071 4068 Fragments arranged by size: 4068 1071 85 BsmI GAATG_Cn' Cuts at: 2735 2828 2735 Size: 93 5131 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2543 3285 3370 4441 1391 Size: 399 262 491 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 491 399 262 85 BspEI T'CCGG_A Cuts at: 4887 4887 Size: 5224 BspHI T'CATG_A Cuts at: 3113 4843 3113 Size: 1730 3494 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2118 2579 844 Size: 1274 461 3489 Fragments arranged by size: 3489 1274 461 BsrBI CCG'CTC Cuts at: 1394 1823 3111 1394 Size: 429 1288 3507 Fragments arranged by size: 3507 1288 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3719 3893 65 Size: 1183 2471 174 1396 Fragments arranged by size: 2471 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2064 3868 4055 1700 Size: 110 254 1804 187 2869 Fragments arranged by size: 2869 1804 254 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5224 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5224 BssSI C'ACGA_G Cuts at: 3284 4296 3284 Size: 1012 4212 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5224 BstBI TT'CG_AA Cuts at: 4028 4028 Size: 5224 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5224 BstYI r'GATC_y Cuts at: 1972 2289 2439 2554 2921 3306 3323 4775 4861 Size: 317 150 115 367 385 17 1452 86 Cuts at: 4861 4873 5219 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 317 150 115 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2293 2323 513 Size: 552 939 289 30 3414 Fragments arranged by size: 3414 939 552 289 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4485 Fragments arranged by size: 4485 683 56 BtsI GCAGTG_nn' Cuts at: 2740 3534 3554 2740 Size: 794 20 4410 Fragments arranged by size: 4410 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5224 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5189 DraI TTT'AAA Cuts at: 2528 2528 Size: 5224 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2216 4231 809 Size: 507 900 2015 1802 Fragments arranged by size: 2015 1802 900 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2671 3560 5189 Size: 54 1425 50 275 18 841 889 1629 Cuts at: 5189 8 Size: 43 Fragments arranged by size: 1629 1425 889 841 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2671 1537 Size: 275 859 4090 Fragments arranged by size: 4090 859 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 3154 1353 Size: 812 989 3423 Fragments arranged by size: 3423 989 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3811 4185 4331 387 Size: 1064 138 2222 374 146 1280 Fragments arranged by size: 2222 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3287 3884 4671 4939 1239 Size: 393 1655 597 787 268 1524 Fragments arranged by size: 1655 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2048 2049 2092 2254 2048 Size: 1 43 162 5018 Fragments arranged by size: 5018 162 43 1 EcoRI G'AATT_C Cuts at: 2533 2911 2533 Size: 378 4846 FspI TGC'GCA Cuts at: 3730 3730 Size: 5224 HaeII r_GCGC'y Cuts at: 1678 2314 4371 1678 Size: 636 2057 2531 Fragments arranged by size: 2531 2057 636 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 3865 3897 3939 Size: 32 135 32 611 32 1650 32 42 Cuts at: 3939 3971 1373 Size: 32 2626 Fragments arranged by size: 2626 1650 611 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2568 2814 669 Size: 988 281 630 246 3079 Fragments arranged by size: 3079 988 630 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5224 HpaI GTT'AAC Cuts at: 2814 2814 Size: 5224 KpnI G_GTAC'C Cuts at: 2549 2549 Size: 5224 MfeI C'AATT_G Cuts at: 2823 2823 Size: 5224 MluI A'CGCG_T Cuts at: 5100 5100 Size: 5224 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2226 2394 4338 4522 1305 Size: 921 168 1944 184 2007 Fragments arranged by size: 2007 1944 921 184 168 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3402 Fragments arranged by size: 3402 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2483 3182 3541 3700 518 Size: 648 843 474 699 359 159 2042 Fragments arranged by size: 2042 843 699 648 474 359 159 MspA1I CmG'CkG Cuts at: 1606 2640 3317 4465 4710 1606 Size: 1034 677 1148 245 2120 Fragments arranged by size: 2120 1148 1034 677 245 NaeI GCC'GGC Cuts at: 2066 2066 Size: 5224 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3733 Fragments arranged by size: 3733 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5224 NgoMIV G'CCGG_C Cuts at: 2064 2064 Size: 5224 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5224 NotI GC'GGCC_GC Cuts at: 2671 2671 Size: 5224 NspI r_CATG'y Cuts at: 1132 1229 2419 2582 4127 1132 Size: 97 1190 163 1545 2229 Fragments arranged by size: 2229 1545 1190 163 97 PasI CC'CwG_GG Cuts at: 1348 2044 1348 Size: 696 4528 PciI A'CATG_T Cuts at: 4123 4123 Size: 5224 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5224 PmeI GTTT'AAAC Cuts at: 2528 2528 Size: 5224 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3293 3325 4806 4838 3293 Size: 32 1481 32 3679 Fragments arranged by size: 3679 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2254 2254 Size: 5224 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5224 PsiI TTA'TAA Cuts at: 2794 2794 Size: 5224 PspOMI G'GGCC_C Cuts at: 2048 2048 Size: 5224 PstI C_TGCA'G Cuts at: 830 2576 830 Size: 1746 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5224 SalI G'TCGA_C Cuts at: 1936 2566 1936 Size: 630 4594 SbfI CC_TGCA'GG Cuts at: 2576 2576 Size: 5224 ScaI AGT'ACT Cuts at: 3472 5184 3472 Size: 1712 3512 SfcI C'TryA_G Cuts at: 826 1045 2572 3707 4388 4579 826 Size: 219 1527 1135 681 191 1471 Fragments arranged by size: 1527 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5224 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5224 SmaI CCC'GGG Cuts at: 1872 2551 1872 Size: 679 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3328 4229 4491 820 Size: 197 926 1385 901 262 1553 Fragments arranged by size: 1553 1385 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3015 493 Size: 2522 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5224 SphI G_CATG'C Cuts at: 1132 2419 2582 1132 Size: 1287 163 3774 Fragments arranged by size: 3774 1287 163 SspI AAT'ATT Cuts at: 5 52 3148 5 Size: 47 3096 2081 Fragments arranged by size: 3096 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5224 StyI C'CwwG_G Cuts at: 513 1065 2004 2663 513 Size: 552 939 659 3074 Fragments arranged by size: 3074 939 659 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 3251 3268 3421 3606 4051 325 Size: 1255 897 774 17 153 185 445 1498 Fragments arranged by size: 1498 1255 897 774 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3470 5182 96 Size: 274 80 33 51 1443 1493 1712 138 Fragments arranged by size: 1712 1493 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2497 2529 5184 5216 2497 Size: 32 2655 32 2505 Fragments arranged by size: 2655 2505 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5224 XbaI T'CTAG_A Cuts at: 2560 2560 Size: 5224 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5224 XmaI C'CCGG_G Cuts at: 1870 2549 1870 Size: 679 4545 XmnI GAAnn'nnTTC Cuts at: 2240 3353 2240 Size: 1113 4111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3032 276 Size: 53 83 186 936 1498 2468 Fragments arranged by size: 2468 1498 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI