################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI BstAPI BstEII BstPI BstUI BstXI CelII CfoI ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MslI MunI MvnI NarI NcoI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AflIII 1019 ACGTGT A^C[GA][CT]GT AlwNI 123 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1303 CCTCAGG CC^T[ACGT]AGG BalI 1082 TGGCCA TGG^CCA BamHI 83 GGATCC G^GATCC BanI 1072 GGTGCC G^G[CT][GA]CC BanII 40 GAGCCC G[GA]GC[CT]^C BmyI 40 GAGCCC G[AGT]GC[ACT]^C BsaAI 1018 TACGTG [CT]AC^GT[GA] Bsp1286I 40 GAGCCC G[AGT]GC[ACT]^C Bsp1407I 47 TGTACA T^GTACA BsrFI 103 GCCGGC [GA]^CCGG[CT] BsrGI 47 TGTACA T^GTACA BstBI 627 TTCGAA TT^CGAA Bsu36I 1303 CCTCAGG CC^T[ACGT]AGG Cfr10I 103 GCCGGC [GA]^CCGG[CT] DrdI 112 GACACCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1094 CCACGG C^C[GA][CT]GG EaeI 1082 TGGCCA [CT]^GGCC[GA] Eco81I 1303 CCTCAGG CC^T[ACGT]AGG EcoRI 1142 GAATTC G^AATTC EcoRV 489 GATATC GAT^ATC EcoT14I 522 CCAAGG C^C[AT][AT]GG FauI 375 GCGGG CCCGC(4/6) MaeII 1019 ACGT A^CGT MluNI 1082 TGGCCA TGG^CCA MscI 1082 TGGCCA TGG^CCA NaeI 103 GCCGGC GCC^GGC NdeI 462 CATATG CA^TATG NgoMIV 103 GCCGGC G^CCGGC NspV 627 TTCGAA TT^CGAA PshAI 92 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1405 CTGCAG CTGCA^G SfiI 1308 GGCCAGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 627 TTCGAA TT^CGAA SspBI 47 TGTACA T^GTACA StyI 522 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 96 GGCGTC G[GA]^CG[CT]C 1590 GACGCC AfaI 48 GTAC GT^AC 1540 GTAC Asp700I 699 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1573 GAAGAAATTC BglI 1075 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1309 GCCAGACAGGC BsaHI 96 GGCGTC G[GA]^CG[CT]C 1590 GACGCC BsaWI 674 ACCGGA [AT]^CCGG[AT] 882 TCCGGT BseRI 251 GAGGAG GAGGAG(10/8) 1393 GAGGAG BsmAI 1588 GAGAC GTCTC(1/5) 1598 GAGAC BsmBI 1588 GAGACG CGTCTC(1/5) 1598 GAGACG Bst1107I 589 GTATAC GTA^TAC 596 GTATAC BstZ17I 589 GTATAC GTA^TAC 596 GTATAC Hin1I 96 GGCGTC G[GA]^CG[CT]C 1590 GACGCC HphI 282 GGTGA GGTGA(8/7) 775 GGTGA MspA1I 337 CAGCTG C[AC]G^C[GT]G 755 CAGCTG PvuII 337 CAGCTG CAG^CTG 755 CAGCTG RsaI 48 GTAC GT^AC 1540 GTAC SfcI 1405 CTGCAG C^T[GA][CT]AG 1568 CTACAG XmnI 699 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1573 GAAGAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccI AciI AcsI AluI AlwI ApoI AvaII BbsI BbvI BcnI BpmI BpuAI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII ItaI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII