Restriction map for
pFN21AE9532
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BseAI BseRI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI MaeI
MamI MfeI MluI MroI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI TfiI Tsp509I TspRI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 478 GACGTC GACGT^C
Acc65I 1095 GGTACC G^GTACC
AflIII 398 ACATGT A^C[GA][CT]GT
AluI 934 AGCT AG^CT
Asp718I 1095 GGTACC G^GTACC
AspHI 682 GTGCTC G[AT]GC[AT]^C
AviII 1046 TGCGCA TGC^GCA
BbeI 127 GGCGCC GGCGC^C
BsaWI 764 ACCGGA [AT]^CCGG[AT]
BsiHKAI 682 GTGCTC G[AT]GC[AT]^C
BsmBI 1090 GAGACG CGTCTC(1/5)
BsmI 357 GAATGC GAATGC(1/-1)
BspHI 710 TCATGA T^CATGA
BspLU11I 398 ACATGT A^CATGT
BsrI 629 ACTGG ACTGG(1/-1)
BstEII 153 GGTCACC G^GT[ACGT]ACC
BstPI 153 GGTCACC G^GT[ACGT]ACC
BstYI 608 AGATCC [GA]^GATC[CT]
DrdI 289 GACACTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 153 GGTCACC G^GT[ACGT]ACC
FspI 1046 TGCGCA TGC^GCA
HaeII 127 GGCGCC [GA]GCGC^[CT]
HincII 928 GTCAAC GT[CT]^[GA]AC
HindII 928 GTCAAC GT[CT]^[GA]AC
HinfI 731 GAGTC G^A[ACGT]TC
KasI 127 GGCGCC G^GCGCC
KpnI 1095 GGTACC GGTAC^C
KspI 1099 CCGCGG CCGC^GG
MaeIII 154 GTCAC ^GT[ACGT]AC
MflI 608 AGATCC [GA]^GATC[CT]
MseI 272 TTAA T^TAA
NarI 127 GGCGCC GG^CGCC
NspI 398 ACATGT [GA]CATG^[CT]
PflMI 855 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 731 GAGTC GAGTC(4/5)
PshAI 828 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 933 CAGCTG CAG^CTG
RcaI 710 TCATGA T^CATGA
SacII 1099 CCGCGG CCGC^GG
SfcI 702 CTACAG C^T[GA][CT]AG
SfiI 516 GGCCATCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 127 GGCGCC GGC^GCC
SmlI 148 CTCAAG C^T[CT][GA]AG
SspI 259 AATATT AAT^ATT
StuI 980 AGGCCT AGG^CCT
Tru9I 272 TTAA T^TAA
Tsp45I 154 GTCAC ^GT[GC]AC
Van91I 855 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 518 CCATCTGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 608 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 219 GTAC GT^AC
1096 GTAC
AocI 228 CCTCAGG CC^T[ACGT]AGG
619 CCTCAGG
ApaI 524 GGGCCC GGGCC^C
633 GGGCCC
AvaII 297 GGTCC G^G[AT]CC
762 GGACC
BalI 515 TGGCCA TGG^CCA
893 TGGCCA
BanI 127 GGCGCC G^G[CT][GA]CC
1095 GGTACC
BbsI 246 GAAGAC GAAGAC(2/6)
841 GTCTTC
BcnI 636 CCCGG CC^[GC]GG
1020 CCGGG
BglI 138 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
517 GCCATCTGGGC
BpuAI 246 GAAGAC GAAGAC(2/6)
841 GTCTTC
BsgI 909 CTGCAC GTGCAG(16/14)
1027 GTGCAG
BsmAI 883 GAGAC GTCTC(1/5)
1090 GAGAC
BspMI 770 ACCTGC ACCTGC(4/8)
1029 GCAGGT
BsrDI 378 CATTGC GCAATG(2/0)
1053 CATTGC
BstAPI 102 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
992 GCAAGTTCTGC
BstXI 428 CCATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1058 CCAAGGACGTGG
Bsu36I 228 CCTCAGG CC^T[ACGT]AGG
619 CCTCAGG
DraII 524 GGGCCCT [GA]G^G[ACGT]CC[CT]
632 GGGGCCC
Eco81I 228 CCTCAGG CC^T[ACGT]AGG
619 CCTCAGG
EcoO109I 524 GGGCCCT [GA]G^G[ACGT]CC[CT]
632 GGGGCCC
EcoT14I 855 CCAAGG C^C[AT][AT]GG
1058 CCAAGG
HgaI 306 GACGC GACGC(5/10)
828 GACGC
MluNI 515 TGGCCA TGG^CCA
893 TGGCCA
MscI 515 TGGCCA TGG^CCA
893 TGGCCA
MspA1I 933 CAGCTG C[AC]G^C[GT]G
1099 CCGCGG
NciI 636 CCCGG CC^[GC]GG
1020 CCGGG
RsaI 219 GTAC GT^AC
1096 GTAC
StyI 855 CCAAGG C^C[AT][AT]GG
1058 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AlwI AvaI
BanII BbvI BmyI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI Ksp632I
MaeII MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TthHB8I