(Circular) (Six-base) MAPSORT of: pFN21AE9532.seq Check: 2392 from: 1 to: 5815 pFN21AE9532 5815 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3025 3025 Size: 5815 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2487 3625 278 Size: 53 83 186 936 951 1138 2468 Fragments arranged by size: 2468 1138 951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3100 3136 3100 Size: 36 5779 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 3878 5262 5530 1632 Size: 410 1836 1384 268 1917 Fragments arranged by size: 1917 1836 1384 410 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 2403 4714 5691 1203 Size: 1200 2311 977 1327 Fragments arranged by size: 2311 1327 1200 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 2165 1166 Size: 999 4816 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 3558 5130 3558 Size: 1572 4243 ApaI G_GGCC'C Cuts at: 2533 2642 2533 Size: 109 5706 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 3124 3350 3502 5611 1493 Size: 1631 226 152 2109 1697 Fragments arranged by size: 2109 1697 1631 226 152 AseI AT'TA_AT Cuts at: 160 1032 4369 160 Size: 872 3337 1606 Fragments arranged by size: 3337 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 2147 2749 2757 3140 1870 Size: 73 204 602 8 383 4545 Fragments arranged by size: 4545 602 383 204 73 8 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 3100 3136 4591 618 Size: 325 217 627 345 968 36 1455 1842 Fragments arranged by size: 1842 1455 968 627 345 325 217 36 BanII G_rGCy'C Cuts at: 1391 2013 2084 2154 2175 2202 2533 2573 2642 Size: 622 71 70 21 27 331 40 69 Cuts at: 2642 2756 3134 1391 Size: 114 378 4072 Fragments arranged by size: 4072 622 378 331 114 71 70 69 40 27 21 BbeI G_GCGC'C Cuts at: 2136 2136 Size: 5815 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 2839 928 Size: 971 359 581 3904 Fragments arranged by size: 3904 971 581 359 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4006 4040 1903 Size: 34 2069 34 3678 Fragments arranged by size: 3678 2069 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2149 2528 4426 Size: 107 122 71 631 605 477 379 1898 Cuts at: 4426 136 Size: 1525 Fragments arranged by size: 1898 1525 631 605 477 379 122 107 71 BglII A'GATC_T Cuts at: 5810 5810 Size: 5815 BlpI GC'TnA_GC Cuts at: 3232 3232 Size: 5815 Bme1580I G_kGCm'C Cuts at: 1790 2330 2479 2533 2642 3876 5032 1790 Size: 540 149 54 109 1234 1156 2573 Fragments arranged by size: 2573 1234 1156 540 149 109 54 BmgBI CAC'GTC Cuts at: 1318 2493 3031 3070 1318 Size: 1175 538 39 4063 Fragments arranged by size: 4063 1175 538 39 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2644 3546 4504 Size: 416 53 83 399 24 1227 902 958 Cuts at: 4504 442 Size: 1753 Fragments arranged by size: 1753 1227 958 902 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4475 1239 Size: 3236 2579 Bpu10I CC'TnA_GC Cuts at: 2078 2078 Size: 5815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2138 3940 4805 5103 2138 Size: 1802 865 298 2850 Fragments arranged by size: 2850 1802 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4478 882 Size: 617 2979 2219 Fragments arranged by size: 2979 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3606 493 Size: 711 2402 2702 Fragments arranged by size: 2702 2402 711 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 2484 2834 3622 Size: 53 83 186 936 600 351 350 788 Cuts at: 3622 4004 4639 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 788 635 600 382 351 350 186 83 53 BsaWI w'CCGG_w Cuts at: 2769 4248 4646 4920 5067 5478 2769 Size: 1479 398 274 147 411 3106 Fragments arranged by size: 3106 1479 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5590 Fragments arranged by size: 5590 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5815 BseYI C'CCAG_C Cuts at: 2866 5018 5514 2866 Size: 2152 496 3167 Fragments arranged by size: 3167 2152 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2899 3053 1163 Size: 573 1163 154 3925 Fragments arranged by size: 3925 1163 573 154 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3265 4026 5054 1540 Size: 275 186 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 3876 3961 5032 2691 Size: 1185 85 1071 3474 Fragments arranged by size: 3474 1185 1071 85 BsmI GAATG_Cn' Cuts at: 2368 3326 3419 2368 Size: 958 93 4764 Fragments arranged by size: 4764 958 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3089 3089 Size: 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2084 2154 2175 2202 2330 2479 Size: 399 223 71 70 21 27 128 149 Cuts at: 2479 2533 2573 2642 2691 2756 3134 3876 3961 Size: 54 40 69 49 65 378 742 85 Cuts at: 3961 5032 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 378 223 149 128 85 71 70 69 65 54 49 40 27 21 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 2715 3704 5434 2715 Size: 989 1730 3096 Fragments arranged by size: 3096 1730 989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2784 3025 3170 844 Size: 1940 241 145 3489 Fragments arranged by size: 3489 1940 241 145 BsrBI CCG'CTC Cuts at: 1394 1823 3702 1394 Size: 429 1879 3507 Fragments arranged by size: 3507 1879 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2382 3057 4310 4484 65 Size: 1183 1134 675 1253 174 1396 Fragments arranged by size: 1396 1253 1183 1134 675 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4459 4646 1700 Size: 110 2649 187 2869 Fragments arranged by size: 2869 2649 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5815 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5815 BssSI C'ACGA_G Cuts at: 3875 4887 3875 Size: 1012 4803 BstAPI GCAn_nnn'nTGC Cuts at: 794 2113 3003 794 Size: 1319 890 3606 Fragments arranged by size: 3606 1319 890 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 2158 1184 Size: 974 4841 BstXI CCAn_nnnn'nTGG Cuts at: 2440 3070 2440 Size: 630 5185 BstYI r'GATC_y Cuts at: 1972 2613 3145 3512 3897 3914 5366 5452 5464 Size: 641 532 367 385 17 1452 86 12 Cuts at: 5464 5810 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 641 532 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2234 2625 2234 Size: 391 5424 BtgI C'CryG_G Cuts at: 513 1065 2004 2128 2731 2895 3104 513 Size: 552 939 124 603 164 209 3224 Fragments arranged by size: 3224 939 603 552 209 164 124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5076 Fragments arranged by size: 5076 683 56 BtsI GCAGTG_nn' Cuts at: 3331 4125 4145 3331 Size: 794 20 5001 Fragments arranged by size: 5001 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 3119 3119 Size: 5815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2300 4822 809 Size: 507 984 2522 1802 Fragments arranged by size: 2522 1802 984 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2289 2351 Size: 54 1425 50 275 18 351 108 62 Cuts at: 2351 2520 2898 3262 4151 5780 8 Size: 169 378 364 889 1629 43 Fragments arranged by size: 1629 1425 889 378 364 351 275 169 108 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3262 1537 Size: 275 1450 4090 Fragments arranged by size: 4090 1450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2586 2672 3745 1353 Size: 668 565 86 1073 3423 Fragments arranged by size: 3423 1073 668 565 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2729 4402 4776 4922 387 Size: 1064 138 1140 1673 374 146 1280 Fragments arranged by size: 1673 1280 1140 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 3878 4475 5262 5530 1239 Size: 393 410 1836 597 787 268 1524 Fragments arranged by size: 1836 1524 787 597 410 393 268 EcoO109I rG'GnC_Cy Cuts at: 2530 2638 2530 Size: 108 5707 EcoRI G'AATT_C Cuts at: 3124 3502 3124 Size: 378 5437 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3007 3007 Size: 5815 FspI TGC'GCA Cuts at: 3053 4321 3053 Size: 1268 4547 HaeII r_GCGC'y Cuts at: 1678 2136 4962 1678 Size: 458 2826 2531 Fragments arranged by size: 2826 2531 458 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2428 2460 4456 4488 4530 Size: 32 135 32 856 32 1996 32 42 Cuts at: 4530 4562 1373 Size: 32 2626 Fragments arranged by size: 2626 1996 856 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2935 3159 3405 669 Size: 988 281 997 224 246 3079 Fragments arranged by size: 3079 997 988 281 246 224 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KasI G'GCGC_C Cuts at: 2132 2132 Size: 5815 KpnI G_GTAC'C Cuts at: 3104 3140 3104 Size: 36 5779 MfeI C'AATT_G Cuts at: 3414 3414 Size: 5815 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2173 4929 5113 1305 Size: 868 2756 184 2007 Fragments arranged by size: 2756 2007 868 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2522 2900 10 Size: 54 1425 343 690 378 2925 Fragments arranged by size: 2925 1425 690 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2246 2438 3773 4132 4291 518 Size: 648 999 81 192 1335 359 159 2042 Fragments arranged by size: 2042 1335 999 648 359 192 159 81 MspA1I CmG'CkG Cuts at: 1606 2940 3106 3231 3908 5056 5301 1606 Size: 1334 166 125 677 1148 245 2120 Fragments arranged by size: 2120 1334 1148 677 245 166 125 NarI GG'CG_CC Cuts at: 2133 2133 Size: 5815 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4324 Fragments arranged by size: 4324 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5815 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 2407 3173 4718 1132 Size: 97 1178 766 1545 2229 Fragments arranged by size: 2229 1545 1178 766 97 PasI CC'CwG_GG Cuts at: 1348 2923 1348 Size: 1575 4240 PciI A'CATG_T Cuts at: 2403 4714 2403 Size: 2311 3504 PflMI CCAn_nnn'nTGG Cuts at: 1108 2866 1108 Size: 1758 4057 PfoI T'CCnGG_A Cuts at: 2967 2967 Size: 5815 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2837 1613 Size: 1224 4591 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PspOMI G'GGCC_C Cuts at: 2529 2638 2529 Size: 109 5706 PstI C_TGCA'G Cuts at: 830 3167 830 Size: 2337 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 PvuII CAG'CTG Cuts at: 2940 2940 Size: 5815 SacII CC_GC'GG Cuts at: 3107 3107 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SfcI C'TryA_G Cuts at: 826 1045 2707 3163 4298 4979 5170 826 Size: 219 1662 456 1135 681 191 1471 Fragments arranged by size: 1662 1471 1135 681 456 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2528 2528 Size: 5815 SfoI GGC'GCC Cuts at: 2134 2134 Size: 5815 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 3919 4820 5082 820 Size: 197 926 210 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 210 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 2266 3739 5 Size: 47 2214 1473 2081 Fragments arranged by size: 2214 2081 1473 47 StuI AGG'CCT Cuts at: 1517 2987 1517 Size: 1470 4345 StyI C'CwwG_G Cuts at: 513 1065 2004 2860 3063 3254 513 Size: 552 939 856 203 191 3074 Fragments arranged by size: 3074 939 856 552 203 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3842 3859 4012 4197 4642 325 Size: 1255 2262 17 153 185 445 1498 Fragments arranged by size: 2262 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4061 5773 96 Size: 274 80 33 51 1443 2084 1712 138 Fragments arranged by size: 2084 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5775 5807 5775 Size: 32 5783 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5815 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2530 2530 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5815 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 3944 3944 Size: 5815 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2485 3623 276 Size: 53 83 186 936 951 1138 2468 Fragments arranged by size: 2468 1138 951 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI