(Circular) (Six-base) MAPSORT of: pFN21AE2246.seq Check: 9067 from: 1 to: 5605 pFN21AE2246 5605 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2948 1937 Size: 1011 4594 Acc65I G'GTAC_C Cuts at: 2617 2926 2617 Size: 309 5296 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2789 3668 5052 5320 1632 Size: 1157 879 1384 268 1917 Fragments arranged by size: 1917 1384 1157 879 268 AfeI AGC'GCT Cuts at: 2264 2888 2264 Size: 624 4981 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 2210 4504 5481 1203 Size: 1007 2294 977 1327 Fragments arranged by size: 2294 1327 1007 977 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 2174 2334 1166 Size: 1008 160 4437 Fragments arranged by size: 4437 1008 160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2192 2332 1184 Size: 1008 140 4457 Fragments arranged by size: 4457 1008 140 AlwNI CAG_nnn'CTG Cuts at: 2627 2698 2842 3348 4920 2627 Size: 71 144 506 1572 3312 Fragments arranged by size: 3312 1572 506 144 71 ApaLI G'TGCA_C Cuts at: 2581 2900 3662 4818 2581 Size: 319 762 1156 3368 Fragments arranged by size: 3368 1156 762 319 ApoI r'AATT_y Cuts at: 1493 2914 3140 3292 5401 1493 Size: 1421 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2425 2711 2861 2930 1870 Size: 73 482 286 150 69 4545 Fragments arranged by size: 4545 482 286 150 73 69 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2601 2634 2601 Size: 33 5572 BamHI G'GATC_C Cuts at: 2935 3302 2935 Size: 367 5238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 2168 2408 2429 2471 Size: 325 217 627 287 94 240 21 42 Cuts at: 2471 2617 2926 4381 618 Size: 146 309 1455 1842 Fragments arranged by size: 1842 1455 627 325 309 287 240 217 146 94 42 21 BanII G_rGCy'C Cuts at: 1391 2796 2924 1391 Size: 1405 128 4072 Fragments arranged by size: 4072 1405 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4634 BbvCI CC'TCA_GC Cuts at: 2606 2628 2606 Size: 22 5583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3497 4707 3497 Size: 1210 4395 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2166 2355 Size: 107 122 71 631 605 337 157 189 Cuts at: 2355 2469 2714 4216 136 Size: 114 245 1502 1525 Fragments arranged by size: 1525 1502 631 605 337 245 189 157 122 114 107 71 BglII A'GATC_T Cuts at: 5600 5600 Size: 5605 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 2162 2432 2465 2585 2904 3666 4822 1790 Size: 372 270 33 120 319 762 1156 2573 Fragments arranged by size: 2573 1156 762 372 319 270 120 33 BmgBI CAC'GTC Cuts at: 1318 2211 1318 Size: 893 4712 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2149 3336 4294 Size: 416 53 83 399 24 732 1187 958 Cuts at: 4294 442 Size: 1753 Fragments arranged by size: 1753 1187 958 732 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2279 2476 2888 4265 1239 Size: 797 243 197 412 1377 2579 Fragments arranged by size: 2579 1377 797 412 243 197 Bpu10I CC'TnA_GC Cuts at: 2606 2628 2606 Size: 22 5583 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4268 882 Size: 617 2769 2219 Fragments arranged by size: 2769 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3396 493 Size: 711 2192 2702 Fragments arranged by size: 2702 2192 711 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3412 3794 4429 275 Size: 53 83 186 936 1879 382 635 1451 Fragments arranged by size: 1879 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4038 4436 4710 4857 5268 4038 Size: 398 274 147 411 4375 Fragments arranged by size: 4375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2866 2896 1178 Size: 30 165 30 1463 30 3887 Fragments arranged by size: 3887 1463 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2315 2318 2645 1186 Size: 1129 3 327 4146 Fragments arranged by size: 4146 1129 327 3 BseYI C'CCAG_C Cuts at: 2435 2727 4808 5304 2435 Size: 292 2081 496 2736 Fragments arranged by size: 2736 2081 496 292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2509 2575 1163 Size: 573 462 311 66 4193 Fragments arranged by size: 4193 573 462 311 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2186 3055 3816 4844 1540 Size: 275 186 185 869 761 1028 2301 Fragments arranged by size: 2301 1028 869 761 275 186 185 BsiHKAI G_wGCw'C Cuts at: 2391 2585 2796 2904 3666 3751 4822 2391 Size: 194 211 108 762 85 1071 3174 Fragments arranged by size: 3174 1071 762 211 194 108 85 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2391 2432 2465 2585 2796 2904 Size: 399 372 229 41 33 120 211 108 Cuts at: 2904 2924 3666 3751 4822 1391 Size: 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 372 229 211 120 108 85 41 33 20 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2960 844 Size: 2116 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2140 2872 3492 1394 Size: 429 317 732 620 3507 Fragments arranged by size: 3507 732 620 429 317 BsrDI GCAATG_nn' Cuts at: 65 1248 4100 4274 65 Size: 1183 2852 174 1396 Fragments arranged by size: 2852 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2071 2077 2354 2411 4249 4436 1700 Size: 110 261 6 277 57 1838 187 2869 Fragments arranged by size: 2869 1838 277 261 187 110 57 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 2589 2698 2842 794 Size: 1795 109 144 3557 Fragments arranged by size: 3557 1795 144 109 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2935 3302 3687 3704 5156 5242 5254 5600 Size: 963 367 385 17 1452 86 12 346 Cuts at: 5600 1972 Size: 1977 Fragments arranged by size: 1977 1452 963 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5053 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 2456 2510 3121 3915 3935 2456 Size: 54 611 794 20 4126 Fragments arranged by size: 4126 794 611 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DraIII CAC_nnn'GTG Cuts at: 2211 2408 2451 2580 2211 Size: 197 43 129 5236 Fragments arranged by size: 5236 197 129 43 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2183 2850 3052 Size: 54 1425 50 275 18 353 667 202 Cuts at: 3052 3941 5570 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 667 353 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2183 3052 1537 Size: 275 371 869 4090 Fragments arranged by size: 4090 869 371 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2566 3535 1353 Size: 1213 969 3423 Fragments arranged by size: 3423 1213 969 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2794 2794 Size: 5605 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2279 2476 2789 2888 3668 4265 Size: 393 404 243 197 313 99 780 597 Cuts at: 4265 5052 5320 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 780 597 404 393 313 268 243 197 99 EcoO109I rG'GnC_Cy Cuts at: 2707 2716 2707 Size: 9 5596 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 2015 2057 2266 2819 2843 2890 4752 1678 Size: 337 42 209 553 24 47 1862 2531 Fragments arranged by size: 2531 1862 553 337 209 47 42 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 4246 4278 4320 Size: 32 135 32 806 32 1836 32 42 Cuts at: 4320 4352 1373 Size: 32 2626 Fragments arranged by size: 2626 1836 806 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2949 3195 669 Size: 988 281 1011 246 3079 Fragments arranged by size: 3079 1011 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2621 2930 2621 Size: 309 5296 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2759 4719 4903 1305 Size: 1454 1960 184 2007 Fragments arranged by size: 2007 1960 1454 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2852 10 Size: 54 1425 343 1020 2763 Fragments arranged by size: 2763 1425 1020 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 2334 3563 3922 4081 518 Size: 648 1008 160 1229 359 159 2042 Fragments arranged by size: 2042 1229 1008 648 359 160 159 MspA1I CmG'CkG Cuts at: 1606 2182 2346 2589 2695 2767 3021 3698 4846 Size: 576 164 243 106 72 254 677 1148 Cuts at: 4846 5091 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 576 254 245 243 164 106 72 NaeI GCC'GGC Cuts at: 2079 2413 2079 Size: 334 5271 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5053 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NgoMIV G'CCGG_C Cuts at: 2077 2411 2077 Size: 334 5271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2963 4508 1132 Size: 97 1734 1545 2229 Fragments arranged by size: 2229 1734 1545 97 PasI CC'CwG_GG Cuts at: 1348 2533 1348 Size: 1185 4420 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5605 PfoI T'CCnGG_A Cuts at: 2667 2667 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PspXI vC'TCGA_Gb Cuts at: 2711 2711 Size: 5605 PstI C_TGCA'G Cuts at: 830 2694 2706 2838 2957 830 Size: 1864 12 132 119 3478 Fragments arranged by size: 3478 1864 132 119 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 PvuII CAG'CTG Cuts at: 2589 2767 2589 Size: 178 5427 SacI G_AGCT'C Cuts at: 2796 2796 Size: 5605 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SapI GCTCTTCn'nnn_ Cuts at: 2566 2566 Size: 5605 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SexAI A'CCwGG_T Cuts at: 2404 2404 Size: 5605 SfcI C'TryA_G Cuts at: 826 1045 2690 2702 2834 2953 4088 4769 4960 Size: 219 1645 12 132 119 1135 681 191 Cuts at: 4960 826 Size: 1471 Fragments arranged by size: 1645 1471 1135 681 219 191 132 119 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2714 2714 Size: 5605 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2427 2863 2932 1872 Size: 555 436 69 4545 Fragments arranged by size: 4545 555 436 69 SmlI C'TyrA_G Cuts at: 820 1017 1943 2711 3709 4610 4872 820 Size: 197 926 768 998 901 262 1553 Fragments arranged by size: 1553 998 926 901 768 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SrfI GCCC'GGGC Cuts at: 2863 2863 Size: 5605 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 2028 1517 Size: 511 5094 StyI C'CwwG_G Cuts at: 513 1065 2741 3044 513 Size: 552 1676 303 3074 Fragments arranged by size: 3074 1676 552 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2119 2342 3632 3649 3802 3987 4432 Size: 1255 539 223 1290 17 153 185 445 Cuts at: 4432 325 Size: 1498 Fragments arranged by size: 1498 1290 1255 539 445 223 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3851 5563 96 Size: 274 80 33 51 1443 1874 1712 138 Fragments arranged by size: 1874 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2866 2898 5565 5597 2866 Size: 32 2667 32 2874 Fragments arranged by size: 2874 2667 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5605 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 2711 1943 Size: 768 4837 XmaI C'CCGG_G Cuts at: 1870 2425 2861 2930 1870 Size: 555 436 69 4545 Fragments arranged by size: 4545 555 436 69 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SfoI SwaI