(Linear) (Six-base) MAPSORT of: KIEE2246.seq Check: 8345 from: 1 to: 900 KIEE2246.orf With 182 enzymes: SgfI * July 15, 2010 18:56 .. Acc65I G'GTAC_C Cuts at: 0 612 900 Size: 612 288 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 784 900 Size: 784 116 AfeI AGC'GCT Cuts at: 0 259 883 900 Size: 259 624 17 Fragments arranged by size: 624 259 17 AflIII A'CryG_T Cuts at: 0 205 900 Size: 205 695 AleI CACnn'nnGTG Cuts at: 0 169 329 900 Size: 169 160 571 Fragments arranged by size: 571 169 160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 187 327 900 Size: 187 140 573 Fragments arranged by size: 573 187 140 AlwNI CAG_nnn'CTG Cuts at: 0 622 693 837 900 Size: 622 71 144 63 Fragments arranged by size: 622 144 71 63 ApaLI G'TGCA_C Cuts at: 0 576 895 900 Size: 576 319 5 Fragments arranged by size: 576 319 5 AvaI C'yCGr_G Cuts at: 0 420 706 856 900 Size: 420 286 150 44 Fragments arranged by size: 420 286 150 44 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 596 629 900 Size: 596 33 271 Fragments arranged by size: 596 271 33 BanI G'GyrC_C Cuts at: 0 69 163 403 424 466 612 900 Size: 69 94 240 21 42 146 288 Fragments arranged by size: 288 240 146 94 69 42 21 BanII G_rGCy'C Cuts at: 0 791 900 Size: 791 109 BbvCI CC'TCA_GC Cuts at: 0 601 623 900 Size: 601 22 277 Fragments arranged by size: 601 277 22 BglI GCCn_nnn'nGGC Cuts at: 0 161 350 464 709 900 Size: 161 189 114 245 191 Fragments arranged by size: 245 191 189 161 114 Bme1580I G_kGCm'C Cuts at: 0 157 427 460 580 899 900 Size: 157 270 33 120 319 1 Fragments arranged by size: 319 270 157 120 33 1 BmgBI CAC'GTC Cuts at: 0 206 900 Size: 206 694 BmrI ACTGGGnnnn_n' Cuts at: 0 144 900 Size: 144 756 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 274 471 883 900 Size: 31 243 197 412 17 Fragments arranged by size: 412 243 197 31 17 Bpu10I CC'TnA_GC Cuts at: 0 601 623 900 Size: 601 22 277 Fragments arranged by size: 601 277 22 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 861 891 900 Size: 861 30 9 Fragments arranged by size: 861 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 310 313 640 900 Size: 310 3 327 260 Fragments arranged by size: 327 310 260 3 BseYI C'CCAG_C Cuts at: 0 430 722 900 Size: 430 292 178 Fragments arranged by size: 430 292 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 504 570 900 Size: 193 311 66 330 Fragments arranged by size: 330 311 193 66 BsiEI CG_ry'CG Cuts at: 0 181 900 Size: 181 719 BsiHKAI G_wGCw'C Cuts at: 0 386 580 791 899 900 Size: 386 194 211 108 1 Fragments arranged by size: 386 211 194 108 1 Bsp1286I G_dGCh'C Cuts at: 0 157 386 427 460 580 791 899 900 Size: 157 229 41 33 120 211 108 1 Fragments arranged by size: 229 211 157 120 108 41 33 1 BsrBI CCG'CTC Cuts at: 0 135 867 900 Size: 135 732 33 Fragments arranged by size: 732 135 33 BsrFI r'CCGG_y Cuts at: 0 66 72 349 406 900 Size: 66 6 277 57 494 Fragments arranged by size: 494 277 66 57 6 BstAPI GCAn_nnn'nTGC Cuts at: 0 584 693 837 900 Size: 584 109 144 63 Fragments arranged by size: 584 144 109 63 BtsI GCAGTG_nn' Cuts at: 0 451 505 900 Size: 451 54 395 Fragments arranged by size: 451 395 54 DraIII CAC_nnn'GTG Cuts at: 0 206 403 446 575 900 Size: 206 197 43 129 325 Fragments arranged by size: 325 206 197 129 43 EaeI y'GGCC_r Cuts at: 0 178 845 900 Size: 178 667 55 Fragments arranged by size: 667 178 55 EagI C'GGCC_G Cuts at: 0 178 900 Size: 178 722 EarI CTCTTCn'nnn_ Cuts at: 0 561 900 Size: 561 339 EcoICRI GAG'CTC Cuts at: 0 789 900 Size: 789 111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 31 274 471 784 883 900 Size: 31 243 197 313 99 17 Fragments arranged by size: 313 243 197 99 31 17 EcoO109I rG'GnC_Cy Cuts at: 0 702 711 900 Size: 702 9 189 Fragments arranged by size: 702 189 9 HaeII r_GCGC'y Cuts at: 0 10 52 261 814 838 885 900 Size: 10 42 209 553 24 47 15 Fragments arranged by size: 553 209 47 42 24 15 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 373 405 900 Size: 373 32 495 Fragments arranged by size: 495 373 32 KpnI G_GTAC'C Cuts at: 0 616 900 Size: 616 284 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 754 900 Size: 754 146 MscI TGG'CCA Cuts at: 0 847 900 Size: 847 53 MslI CAynn'nnrTG Cuts at: 0 169 329 900 Size: 169 160 571 Fragments arranged by size: 571 169 160 MspA1I CmG'CkG Cuts at: 0 177 341 584 690 762 900 Size: 177 164 243 106 72 138 Fragments arranged by size: 243 177 164 138 106 72 NaeI GCC'GGC Cuts at: 0 74 408 900 Size: 74 334 492 Fragments arranged by size: 492 334 74 NgoMIV G'CCGG_C Cuts at: 0 72 406 900 Size: 72 334 494 Fragments arranged by size: 494 334 72 PasI CC'CwG_GG Cuts at: 0 528 900 Size: 528 372 PfoI T'CCnGG_A Cuts at: 0 662 900 Size: 662 238 PspXI vC'TCGA_Gb Cuts at: 0 706 900 Size: 706 194 PstI C_TGCA'G Cuts at: 0 689 701 833 900 Size: 689 12 132 67 Fragments arranged by size: 689 132 67 12 PvuII CAG'CTG Cuts at: 0 584 762 900 Size: 584 178 138 Fragments arranged by size: 584 178 138 SacI G_AGCT'C Cuts at: 0 791 900 Size: 791 109 SapI GCTCTTCn'nnn_ Cuts at: 0 561 900 Size: 561 339 SexAI A'CCwGG_T Cuts at: 0 399 900 Size: 399 501 SfcI C'TryA_G Cuts at: 0 685 697 829 900 Size: 685 12 132 71 Fragments arranged by size: 685 132 71 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 709 900 Size: 709 191 SmaI CCC'GGG Cuts at: 0 422 858 900 Size: 422 436 42 Fragments arranged by size: 436 422 42 SmlI C'TyrA_G Cuts at: 0 706 900 Size: 706 194 SrfI GCCC'GGGC Cuts at: 0 858 900 Size: 858 42 StuI AGG'CCT Cuts at: 0 23 900 Size: 23 877 StyI C'CwwG_G Cuts at: 0 736 900 Size: 736 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 114 337 900 Size: 114 223 563 Fragments arranged by size: 563 223 114 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 861 893 900 Size: 861 32 7 Fragments arranged by size: 861 32 7 XhoI C'TCGA_G Cuts at: 0 706 900 Size: 706 194 XmaI C'CCGG_G Cuts at: 0 420 856 900 Size: 420 436 44 Fragments arranged by size: 436 420 44 Enzymes that do cut: Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaLI AvaI BaeI BanI BanII BbvCI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BstAPI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV PasI PfoI PspXI PstI PvuII SacI SapI SexAI SfcI SfiI SmaI SmlI SrfI StuI StyI TaqII TstI XhoI XmaI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI XcmI XmnI ZraI