Restriction map for
pFN21AB9520
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AlwNI AocI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I EarI
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
Ksp632I MaeI MfeI MflI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
Tth111I XbaI XcmI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Alw44I 351 GTGCAC G^TGCAC
Aor51HI 1037 AGCGCT AGC^GCT
ApaLI 351 GTGCAC G^TGCAC
Asp700I 204 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 250 GGCGCC G^G[CT][GA]CC
BbeI 250 GGCGCC GGCGC^C
BclI 1133 TGATCA T^GATCA
BsaAI 280 TACGTG [CT]AC^GT[GA]
BsaBI 1131 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 417 GGTCTC GGTCTC(1/5)
BsgI 814 CTGCAC GTGCAG(16/14)
BspMI 344 ACCTGC ACCTGC(4/8)
BsrBI 413 GAGCGG CCGCTC(-3/-3)
BsrFI 718 GCCGGC [GA]^CCGG[CT]
BstEII 405 GGTCACC G^GT[ACGT]ACC
BstPI 405 GGTCACC G^GT[ACGT]ACC
Cfr10I 718 GCCGGC [GA]^CCGG[CT]
DpnII 1134 GATC ^GATC
DrdI 238 GACTTCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1037 AGCGCT AGC^GCT
EcoO65I 405 GGTCACC G^GT[ACGT]ACC
EcoT14I 1152 CCATGG C^C[AT][AT]GG
FbaI 1133 TGATCA T^GATCA
HincII 1219 GTCAAC GT[CT]^[GA]AC
HindII 1219 GTCAAC GT[CT]^[GA]AC
HinfI 184 GACTC G^A[ACGT]TC
KasI 250 GGCGCC G^GCGCC
KspI 101 CCGCGG CCGC^GG
MaeII 281 ACGT A^CGT
MamI 1131 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1134 GATC ^GATC
MseI 650 TTAA T^TAA
NaeI 718 GCCGGC GCC^GGC
NarI 250 GGCGCC GG^CGCC
NcoI 1152 CCATGG C^CATGG
NdeII 1134 GATC ^GATC
NgoMIV 718 GCCGGC G^CCGGC
PflMI 760 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 184 GACTC GAGTC(4/5)
SacII 101 CCGCGG CCGC^GG
Sau3AI 1134 GATC ^GATC
SfoI 250 GGCGCC GGC^GCC
StyI 1152 CCATGG C^C[AT][AT]GG
Tru9I 650 TTAA T^TAA
Van91I 760 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 204 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcyI 250 GGCGCC G[GA]^CG[CT]C
981 GGCGTC
ApaI 428 GGGCCC GGGCC^C
1112 GGGCCC
BsaHI 250 GGCGCC G[GA]^CG[CT]C
981 GGCGTC
BseRI 357 CTCCTC GAGGAG(10/8)
927 CTCCTC
BsmAI 418 GTCTC GTCTC(1/5)
1050 GTCTC
BsrI 584 ACTGG ACTGG(1/-1)
1175 ACTGG
BstXI 140 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1070 CCAAGTCGCTGG
DraII 311 GGGGCCT [GA]G^G[ACGT]CC[CT]
1112 GGGCCCC
EagI 306 CGGCCG C^GGCCG
839 CGGCCG
EclXI 306 CGGCCG C^GGCCG
839 CGGCCG
Eco52I 306 CGGCCG C^GGCCG
839 CGGCCG
EcoO109I 311 GGGGCCT [GA]G^G[ACGT]CC[CT]
1112 GGGCCCC
Hin1I 250 GGCGCC G[GA]^CG[CT]C
981 GGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BalI BanII BbvCI
BbvI BcnI BglI BmyI BpmI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DsaI EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeIII MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PaeR7I RsaI Sau96I ScrFI
SfaNI SmlI TaqI TseI Tsp45I
TspRI TthHB8I XhoI