Restriction map for
pFN21AB9520
(Linear) (Six-base) MAPSORT of: KIBB9520.seq Check: 1979 from: 1 to: 1335
KIBB9520.orf
With 182 enzymes: SgfI *
November 2, 2010 17:11 ..
AfeI AGC'GCT
Cuts at: 0 1039 1335
Size: 1039 296
ApaI G_GGCC'C
Cuts at: 0 432 1116 1335
Size: 432 684 219
Fragments arranged by size:
684 432 219
ApaLI G'TGCA_C
Cuts at: 0 351 1335
Size: 351 984
AvaI C'yCGr_G
Cuts at: 0 186 410 550 707 724 736 871 930
Size: 186 224 140 157 17 12 135 59
Cuts at: 930 1052 1335
Size: 122 283
Fragments arranged by size:
283 224 186 157 140 135 122 59
17 12
BanI G'GyrC_C
Cuts at: 0 250 1335
Size: 250 1085
BanII G_rGCy'C
Cuts at: 0 432 708 731 970 1106 1116 1335
Size: 432 276 23 239 136 10 219
Fragments arranged by size:
432 276 239 219 136 23 10
BbeI G_GCGC'C
Cuts at: 0 254 1335
Size: 254 1081
BbvCI CC'TCA_GC
Cuts at: 0 634 655 851 1335
Size: 634 21 196 484
Fragments arranged by size:
634 484 196 21
BclI T'GATC_A
Cuts at: 0 1133 1335
Size: 1133 202
BglI GCCn_nnn'nGGC
Cuts at: 0 21 178 862 1213 1335
Size: 21 157 684 351 122
Fragments arranged by size:
684 351 157 122 21
Bme1580I G_kGCm'C
Cuts at: 0 355 432 797 940 1116 1335
Size: 355 77 365 143 176 219
Fragments arranged by size:
365 355 219 176 143 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 897 1018 1335
Size: 634 263 121 317
Fragments arranged by size:
634 317 263 121
Bpu10I CC'TnA_GC
Cuts at: 0 514 634 655 851 1335
Size: 514 120 21 196 484
Fragments arranged by size:
514 484 196 120 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1335
Size: 943 392
BsaI GGTCTCn'nnnn_
Cuts at: 0 423 1335
Size: 423 912
BsaAI yAC'GTr
Cuts at: 0 282 1335
Size: 282 1053
BsaBI GATnn'nnATC
Cuts at: 0 1135 1335
Size: 1135 200
BsaHI Gr'CG_yC
Cuts at: 0 251 982 1335
Size: 251 731 353
Fragments arranged by size:
731 353 251
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 1171 1201 1335
Size: 514 30 627 30 134
Fragments arranged by size:
627 514 134 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 348 918 1335
Size: 348 570 417
Fragments arranged by size:
570 417 348
BseYI C'CCAG_C
Cuts at: 0 147 431 518 743 773 1034 1109 1335
Size: 147 284 87 225 30 261 75 226
Fragments arranged by size:
284 261 226 225 147 87 75 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1335
Size: 799 536
BsiEI CG_ry'CG
Cuts at: 0 309 393 842 1335
Size: 309 84 449 493
Fragments arranged by size:
493 449 309 84
BsiHKAI G_wGCw'C
Cuts at: 0 355 380 1325 1335
Size: 355 25 945 10
Fragments arranged by size:
945 355 25 10
Bsp1286I G_dGCh'C
Cuts at: 0 355 380 432 708 731 797 940 970
Size: 355 25 52 276 23 66 143 30
Cuts at: 970 1106 1116 1325 1335
Size: 136 10 209 10
Fragments arranged by size:
355 276 209 143 136 66 52 30
25 23 10 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 1335
Size: 353 982
BsrBI CCG'CTC
Cuts at: 0 415 1335
Size: 415 920
BsrFI r'CCGG_y
Cuts at: 0 718 1335
Size: 718 617
BssHII G'CGCG_C
Cuts at: 0 6 8 76 1335
Size: 6 2 68 1259
Fragments arranged by size:
1259 68 6 2
BstEII G'GTnAC_C
Cuts at: 0 405 1335
Size: 405 930
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 1077 1335
Size: 147 930 258
Fragments arranged by size:
930 258 147
BtgI C'CryG_G
Cuts at: 0 101 140 896 1152 1335
Size: 101 39 756 256 183
Fragments arranged by size:
756 256 183 101 39
BtsI GCAGTG_nn'
Cuts at: 0 461 558 1158 1323 1335
Size: 461 97 600 165 12
Fragments arranged by size:
600 461 165 97 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 244 1335
Size: 244 1091
EaeI y'GGCC_r
Cuts at: 0 110 137 179 306 839 1067 1079 1155
Size: 110 27 42 127 533 228 12 76
Cuts at: 1155 1335
Size: 180
Fragments arranged by size:
533 228 180 127 110 76 42 27
12
EagI C'GGCC_G
Cuts at: 0 306 839 1335
Size: 306 533 496
Fragments arranged by size:
533 496 306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1246 1335
Size: 1246 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 897 1018 1335
Size: 634 263 121 317
Fragments arranged by size:
634 317 263 121
EcoO109I rG'GnC_Cy
Cuts at: 0 312 1113 1335
Size: 312 801 222
Fragments arranged by size:
801 312 222
HaeII r_GCGC'y
Cuts at: 0 46 127 254 851 1041 1335
Size: 46 81 127 597 190 294
Fragments arranged by size:
597 294 190 127 81 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 494 514 526 546 1335
Size: 494 20 12 20 789
Fragments arranged by size:
789 494 20 20 12
HincII GTy'rAC
Cuts at: 0 1221 1335
Size: 1221 114
KasI G'GCGC_C
Cuts at: 0 250 1335
Size: 250 1085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1335
Size: 260 1075
MscI TGG'CCA
Cuts at: 0 139 1069 1157 1335
Size: 139 930 88 178
Fragments arranged by size:
930 178 139 88
MslI CAynn'nnrTG
Cuts at: 0 563 821 1012 1335
Size: 563 258 191 323
Fragments arranged by size:
563 323 258 191
MspA1I CmG'CkG
Cuts at: 0 27 103 296 813 1335
Size: 27 76 193 517 522
Fragments arranged by size:
522 517 193 76 27
NaeI GCC'GGC
Cuts at: 0 720 1335
Size: 720 615
NarI GG'CG_CC
Cuts at: 0 251 1335
Size: 251 1084
NcoI C'CATG_G
Cuts at: 0 1152 1335
Size: 1152 183
NgoMIV G'CCGG_C
Cuts at: 0 718 1335
Size: 718 617
PflMI CCAn_nnn'nTGG
Cuts at: 0 766 1335
Size: 766 569
PspOMI G'GGCC_C
Cuts at: 0 428 1112 1335
Size: 428 684 223
Fragments arranged by size:
684 428 223
PspXI vC'TCGA_Gb
Cuts at: 0 186 930 1335
Size: 186 744 405
Fragments arranged by size:
744 405 186
SacII CC_GC'GG
Cuts at: 0 104 1335
Size: 104 1231
SfoI GGC'GCC
Cuts at: 0 252 1335
Size: 252 1083
SmlI C'TyrA_G
Cuts at: 0 186 707 930 958 1335
Size: 186 521 223 28 377
Fragments arranged by size:
521 377 223 186 28
StyI C'CwwG_G
Cuts at: 0 1152 1335
Size: 1152 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 393 468 820 921 1048 1335
Size: 214 179 75 352 101 127 287
Fragments arranged by size:
352 287 214 179 127 101 75
XhoI C'TCGA_G
Cuts at: 0 186 707 930 1335
Size: 186 521 223 405
Fragments arranged by size:
521 405 223 186
XmnI GAAnn'nnTTC
Cuts at: 0 208 1335
Size: 208 1127
Enzymes that do cut:
AfeI ApaI ApaLI AvaI BanI BanII BbeI BbvCI
BclI BglI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssHII BstEII BstXI BtgI
BtsI DrdI EaeI EagI EciI Eco57MI EcoO109I HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV PflMI PspOMI PspXI SacII SfoI
SmlI StyI TaqII XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EarI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XmaI ZraI