Restriction map for
pFN21AB9741
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AocI Aor51HI AscI AseI
AsnI AvrII BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglII
BlnI BpuAI BsaAI BsaBI BseAI
BseRI BsiWI BspDI BspEI BspHI
BspLU11I BsrDI BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KasI MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI TfiI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 544 GGTACC G^GTACC
AccI 280 GTCTAC GT^[AC][GT]AC
AhdI 379 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 722 GTGCAC G^TGCAC
ApaI 349 GGGCCC GGGCC^C
ApaLI 722 GTGCAC G^TGCAC
Asp700I 252 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 544 GGTACC G^GTACC
AspEI 379 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 126 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 170 TGCGCA TGC^GCA
BamHI 209 GGATCC G^GATCC
BclI 866 TGATCA T^GATCA
BlpI 1176 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1176 GCTGAGC GC^T[ACGT]AGC
BsaWI 1149 TCCGGT [AT]^CCGG[AT]
BsiEI 1444 CGGCCG CG[GA][CT]^CG
BsmBI 403 GAGACG CGTCTC(1/5)
BsmI 940 GCATTC GAATGC(1/-1)
BspMI 173 GCAGGT ACCTGC(4/8)
BsrBI 1179 GAGCGG CCGCTC(-3/-3)
BstXI 1367 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 209 GGATCC [GA]^GATC[CT]
CelII 1176 GCTGAGC GC^T[ACGT]AGC
EagI 1444 CGGCCG C^GGCCG
Eam1105I 379 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1444 CGGCCG C^GGCCG
Eco52I 1444 CGGCCG C^GGCCG
EcoNI 561 CCTTATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 445 GAATTC G^AATTC
FbaI 866 TGATCA T^GATCA
FspI 170 TGCGCA TGC^GCA
KpnI 544 GGTACC GGTAC^C
KspI 1285 CCGCGG CCGC^GG
MflI 209 GGATCC [GA]^GATC[CT]
MseI 1058 TTAA T^TAA
NspI 216 GCATGC [GA]CATG^[CT]
PflFI 126 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1207 GAGCTC GAGCT^C
SacII 1285 CCGCGG CCGC^GG
SapI 1392 GCTCTTC GCTCTTC(1/4)
SbfI 60 CCTGCAGG CCTGCA^GG
SexAI 127 ACCAGGT A^CC[AT]GGT
SfiI 708 GGCCTTTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 114 CCCGGG CCC^GGG
SmlI 1237 CTCAAG C^T[CT][GA]AG
SphI 216 GCATGC GCATG^C
Sse8387I 60 CCTGCAGG CCTGCA^GG
StuI 1019 AGGCCT AGG^CCT
Tru9I 1058 TTAA T^TAA
Tth111I 126 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 209 GGATCC [GA]^GATC[CT]
XmaCI 114 CCCGGG C^CCGGG
XmaI 114 CCCGGG C^CCGGG
XmnI 252 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 1459 GGCGTC G[GA]^CG[CT]C
1480 GACGCC
BalI 716 TGGCCA TGG^CCA
1196 TGGCCA
BfaI 1171 CTAG C^TAG
1403 CTAG
BsaHI 1459 GGCGTC G[GA]^CG[CT]C
1480 GACGCC
BsaI 642 GGTCTC GGTCTC(1/5)
675 GGTCTC
Bsp1407I 467 TGTACA T^GTACA
1274 TGTACA
BsrFI 844 ACCGGC [GA]^CCGG[CT]
1140 GCCGGT
BsrGI 467 TGTACA T^GTACA
1274 TGTACA
BssHII 1347 GCGCGC G^CGCGC
1380 GCGCGC
BstEII 840 GGTCACC G^GT[ACGT]ACC
1450 GGTCACC
BstPI 840 GGTCACC G^GT[ACGT]ACC
1450 GGTCACC
Cfr10I 844 ACCGGC [GA]^CCGG[CT]
1140 GCCGGT
EcoO65I 840 GGTCACC G^GT[ACGT]ACC
1450 GGTCACC
HaeII 394 AGCGCC [GA]GCGC^[CT]
1337 GGCGCT
Hin1I 1459 GGCGTC G[GA]^CG[CT]C
1480 GACGCC
MaeI 1171 CTAG C^TAG
1403 CTAG
MluNI 716 TGGCCA TGG^CCA
1196 TGGCCA
MscI 716 TGGCCA TGG^CCA
1196 TGGCCA
PvuII 82 CAGCTG CAG^CTG
925 CAGCTG
SspBI 467 TGTACA T^GTACA
1274 TGTACA
TaqI 422 TCGA T^CGA
577 TCGA
TthHB8I 422 TCGA T^CGA
577 TCGA
XcmI 750 CCACCTGGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
995 CCACAGAGAAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I Tsp509I TspRI