Restriction map for
pFN21AB9741
(Linear) (Six-base) MAPSORT of: KIBB9741.seq Check: 3762 from: 1 to: 1551
KIBB9741.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 164 1551
Size: 164 1387
AccI GT'mk_AC
Cuts at: 0 281 1551
Size: 281 1270
Acc65I G'GTAC_C
Cuts at: 0 544 1551
Size: 544 1007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1095 1551
Size: 1095 456
AhdI GACnn_n'nnGTC
Cuts at: 0 384 1551
Size: 384 1167
AleI CACnn'nnGTG
Cuts at: 0 729 935 1150 1551
Size: 729 206 215 401
Fragments arranged by size:
729 401 215 206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 196 953 1551
Size: 196 757 598
Fragments arranged by size:
757 598 196
AlwNI CAG_nnn'CTG
Cuts at: 0 414 603 1334 1551
Size: 414 189 731 217
Fragments arranged by size:
731 414 217 189
ApaI G_GGCC'C
Cuts at: 0 353 1551
Size: 353 1198
ApaLI G'TGCA_C
Cuts at: 0 722 1551
Size: 722 829
ApoI r'AATT_y
Cuts at: 0 417 445 1102 1551
Size: 417 28 657 449
Fragments arranged by size:
657 449 417 28
AvaI C'yCGr_G
Cuts at: 0 114 288 1465 1551
Size: 114 174 1177 86
Fragments arranged by size:
1177 174 114 86
BamHI G'GATC_C
Cuts at: 0 209 1551
Size: 209 1342
BanI G'GyrC_C
Cuts at: 0 176 544 976 1087 1152 1305 1551
Size: 176 368 432 111 65 153 246
Fragments arranged by size:
432 368 246 176 153 111 65
BanII G_rGCy'C
Cuts at: 0 353 917 1053 1211 1238 1285 1394 1472
Size: 353 564 136 158 27 47 109 78
Cuts at: 1472 1551
Size: 79
Fragments arranged by size:
564 353 158 136 109 79 78 47
27
BclI T'GATC_A
Cuts at: 0 866 1551
Size: 866 685
BglI GCCn_nnn'nGGC
Cuts at: 0 116 226 715 1551
Size: 116 110 489 836
Fragments arranged by size:
836 489 116 110
BlpI GC'TnA_GC
Cuts at: 0 1177 1551
Size: 1177 374
Bme1580I G_kGCm'C
Cuts at: 0 121 353 726 767 1551
Size: 121 232 373 41 784
Fragments arranged by size:
784 373 232 121 41
BmgBI CAC'GTC
Cuts at: 0 1194 1257 1314 1551
Size: 1194 63 57 237
Fragments arranged by size:
1194 237 63 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 1551
Size: 936 615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 423 1156 1225 1516 1551
Size: 321 102 733 69 291 35
Fragments arranged by size:
733 321 291 102 69 35
Bpu10I CC'TnA_GC
Cuts at: 0 55 1551
Size: 55 1496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1551
Size: 1222 329
BsaI GGTCTCn'nnnn_
Cuts at: 0 648 681 1551
Size: 648 33 870
Fragments arranged by size:
870 648 33
BsaHI Gr'CG_yC
Cuts at: 0 1460 1481 1551
Size: 1460 21 70
Fragments arranged by size:
1460 70 21
BsaWI w'CCGG_w
Cuts at: 0 1149 1551
Size: 1149 402
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 843 873 1551
Size: 843 30 678
Fragments arranged by size:
843 678 30
BseYI C'CCAG_C
Cuts at: 0 540 923 1551
Size: 540 383 628
Fragments arranged by size:
628 540 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 844 913 1551
Size: 819 25 69 638
Fragments arranged by size:
819 638 69 25
BsiEI CG_ry'CG
Cuts at: 0 1447 1551
Size: 1447 104
BsiHKAI G_wGCw'C
Cuts at: 0 146 726 1211 1551
Size: 146 580 485 340
Fragments arranged by size:
580 485 340 146
BsmI GAATG_Cn'
Cuts at: 0 940 1551
Size: 940 611
BsmBI CGTCTCn'nnnn_
Cuts at: 0 397 1551
Size: 397 1154
Bsp1286I G_dGCh'C
Cuts at: 0 121 146 353 726 767 917 1053 1211
Size: 121 25 207 373 41 150 136 158
Cuts at: 1211 1238 1285 1394 1472 1551
Size: 27 47 109 78 79
Fragments arranged by size:
373 207 158 150 136 121 109 79
78 47 41 27 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 1551
Size: 164 1387
BsrBI CCG'CTC
Cuts at: 0 1181 1551
Size: 1181 370
BsrFI r'CCGG_y
Cuts at: 0 844 1140 1551
Size: 844 296 411
Fragments arranged by size:
844 411 296
BsrGI T'GTAC_A
Cuts at: 0 467 1274 1551
Size: 467 807 277
Fragments arranged by size:
807 467 277
BssHII G'CGCG_C
Cuts at: 0 1347 1380 1551
Size: 1347 33 171
Fragments arranged by size:
1347 171 33
BstEII G'GTnAC_C
Cuts at: 0 840 1450 1551
Size: 840 610 101
Fragments arranged by size:
840 610 101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1374 1551
Size: 1374 177
BstYI r'GATC_y
Cuts at: 0 209 1551
Size: 209 1342
BtgI C'CryG_G
Cuts at: 0 353 1212 1285 1551
Size: 353 859 73 266
Fragments arranged by size:
859 353 266 73
EaeI y'GGCC_r
Cuts at: 0 716 1196 1444 1551
Size: 716 480 248 107
Fragments arranged by size:
716 480 248 107
EagI C'GGCC_G
Cuts at: 0 1444 1551
Size: 1444 107
EarI CTCTTCn'nnn_
Cuts at: 0 655 1399 1543 1551
Size: 655 744 144 8
Fragments arranged by size:
744 655 144 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 120 1551
Size: 120 1431
EcoICRI GAG'CTC
Cuts at: 0 1209 1551
Size: 1209 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 423 1095 1156 1225 1516 1551
Size: 321 102 672 61 69 291 35
Fragments arranged by size:
672 321 291 102 69 61 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 565 1551
Size: 565 986
EcoO109I rG'GnC_Cy
Cuts at: 0 292 349 707 1551
Size: 292 57 358 844
Fragments arranged by size:
844 358 292 57
EcoRI G'AATT_C
Cuts at: 0 445 1551
Size: 445 1106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 1551
Size: 276 1275
FspI TGC'GCA
Cuts at: 0 172 1551
Size: 172 1379
HaeII r_GCGC'y
Cuts at: 0 398 1341 1551
Size: 398 943 210
Fragments arranged by size:
943 398 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 751 783 1491 1523 1551
Size: 370 32 349 32 708 32 28
Fragments arranged by size:
708 370 349 32 32 32 28
KpnI G_GTAC'C
Cuts at: 0 548 1551
Size: 548 1003
MscI TGG'CCA
Cuts at: 0 718 1198 1551
Size: 718 480 353
Fragments arranged by size:
718 480 353
MslI CAynn'nnrTG
Cuts at: 0 729 935 1150 1551
Size: 729 206 215 401
Fragments arranged by size:
729 401 215 206
MspA1I CmG'CkG
Cuts at: 0 84 927 1287 1551
Size: 84 843 360 264
Fragments arranged by size:
843 360 264 84
NspI r_CATG'y
Cuts at: 0 220 1551
Size: 220 1331
PfoI T'CCnGG_A
Cuts at: 0 1493 1551
Size: 1493 58
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 1551
Size: 241 32 1278
Fragments arranged by size:
1278 241 32
PspOMI G'GGCC_C
Cuts at: 0 349 1551
Size: 349 1202
PstI C_TGCA'G
Cuts at: 0 65 89 185 1330 1551
Size: 65 24 96 1145 221
Fragments arranged by size:
1145 221 96 65 24
PvuII CAG'CTG
Cuts at: 0 84 927 1551
Size: 84 843 624
Fragments arranged by size:
843 624 84
SacI G_AGCT'C
Cuts at: 0 1211 1551
Size: 1211 340
SacII CC_GC'GG
Cuts at: 0 1288 1551
Size: 1288 263
SapI GCTCTTCn'nnn_
Cuts at: 0 1399 1551
Size: 1399 152
SbfI CC_TGCA'GG
Cuts at: 0 65 1551
Size: 65 1486
SexAI A'CCwGG_T
Cuts at: 0 127 1551
Size: 127 1424
SfcI C'TryA_G
Cuts at: 0 61 85 181 1326 1551
Size: 61 24 96 1145 225
Fragments arranged by size:
1145 225 96 61 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 715 1551
Size: 715 836
SmaI CCC'GGG
Cuts at: 0 116 1551
Size: 116 1435
SmlI C'TyrA_G
Cuts at: 0 1237 1551
Size: 1237 314
SphI G_CATG'C
Cuts at: 0 220 1551
Size: 220 1331
StuI AGG'CCT
Cuts at: 0 1021 1551
Size: 1021 530
StyI C'CwwG_G
Cuts at: 0 221 774 1034 1551
Size: 221 553 260 517
Fragments arranged by size:
553 517 260 221
TatI w'GTAC_w
Cuts at: 0 467 1274 1551
Size: 467 807 277
Fragments arranged by size:
807 467 277
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 843 875 1551
Size: 843 32 676
Fragments arranged by size:
843 676 32
Tth111I GACn'n_nGTC
Cuts at: 0 129 1551
Size: 129 1422
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 757 1002 1551
Size: 757 245 549
Fragments arranged by size:
757 549 245
XmaI C'CCGG_G
Cuts at: 0 114 1551
Size: 114 1437
XmnI GAAnn'nnTTC
Cuts at: 0 256 1551
Size: 256 1295
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BclI
BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII
BstEII BstXI BstYI BtgI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I KpnI MscI MslI MspA1I NspI PfoI PpiI
PspOMI PstI PvuII SacI SacII SapI SbfI SexAI
SfcI SfiI SmaI SmlI SphI StuI StyI TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AloI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI
BciVI BfrBI BglII BmrI BmtI BsaAI BsaBI BseRI
BsiWI BspEI BspHI BsrDI BssSI BstAPI BstBI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoRV FseI FspAI HincII HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI ScaI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TaqII XbaI XhoI ZraI