################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccI AccIII AclI AcyI AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspHI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KspI MaeI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 35 GGTACC G^GTACC AcsI 58 GAATTC [GA]^AATT[CT] AflII 1186 CTTAAG C^TTAAG AflIII 665 ACGTGT A^C[GA][CT]GT AhdI 1369 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 58 GAATTC [GA]^AATT[CT] Asp718I 35 GGTACC G^GTACC AspEI 1369 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 169 GTCTTC GAAGAC(2/6) BfrI 1186 CTTAAG C^TTAAG BpuAI 169 GTCTTC GAAGAC(2/6) BsaAI 664 TACGTG [CT]AC^GT[GA] BsaI 99 GAGACC GGTCTC(1/5) BseRI 16 CTCCTC GAGGAG(10/8) BsgI 66 CTGCAC GTGCAG(16/14) Bsp1407I 527 TGTACA T^GTACA BsrBI 1080 CCGCTC CCGCTC(-3/-3) BsrDI 1307 CATTGC GCAATG(2/0) BsrGI 527 TGTACA T^GTACA BstXI 1445 CCAACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 1411 CTGAG C^T[ACGT]AG EagI 810 CGGCCG C^GGCCG Eam1105I 1369 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 810 CGGCCG C^GGCCG Eco52I 810 CGGCCG C^GGCCG EcoNI 684 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 58 GAATTC G^AATTC HgaI 534 GACGC GACGC(5/10) HincII 1219 GTCAAC GT[CT]^[GA]AC HindII 1219 GTCAAC GT[CT]^[GA]AC HphI 605 TCACC GGTGA(8/7) KpnI 35 GGTACC GGTAC^C NcoI 1364 CCATGG C^CATGG PshAI 634 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 894 GCTCTTC GCTCTTC(1/4) SfiI 1143 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 454 CCCGGG CCC^GGG SspBI 527 TGTACA T^GTACA TfiI 1332 GAATC G^A[AT]TC Tsp509I 59 AATT ^AATT XcmI 113 CCAGGCCAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 454 CCCGGG C^CCGGG XmaI 454 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 991 CGCG CG^CG 1406 CGCG AlwNI 410 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG 1348 CAGACCCTG ApaI 457 GGGCCC GGGCC^C 1142 GGGCCC Asp700I 165 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 891 GAAGCTCTTC AspI 534 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1474 GACATGGTC AvaI 454 CCCGGG C^[CT]CG[GA]G 1372 CCCGAG BsiEI 810 CGGCCG CG[GA][CT]^CG 987 CGACCG BsmAI 3 GAGAC GTCTC(1/5) 99 GAGAC BsoBI 454 CCCGGG C^[CT]CG[GA]G 1372 CCCGAG BspMI 1390 ACCTGC ACCTGC(4/8) 1002 GCAGGT BsrFI 812 GCCGGC [GA]^CCGG[CT] 819 GCCGGC BsrI 1149 ACTGG ACTGG(1/-1) 1247 ACTGG BstUI 991 CGCG CG^CG 1406 CGCG Cfr10I 812 GCCGGC [GA]^CCGG[CT] 819 GCCGGC EarI 895 CTCTTC CTCTTC(1/4) 1429 CTCTTC Ksp632I 895 CTCTTC CTCTTC(1/4) 1429 CTCTTC MaeIII 185 GTCAC ^GT[ACGT]AC 364 GTGAC MslI 187 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1476 CATGGTCGTG MvnI 991 CGCG CG^CG 1406 CGCG NaeI 812 GCCGGC GCC^GGC 819 GCCGGC NgoMIV 812 GCCGGC G^CCGGC 819 GCCGGC NspI 515 ACATGC [GA]CATG^[CT] 1215 GCATGT PflFI 534 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1474 GACATGGTC PpuMI 639 AGGTCCC [GA]G^G[AT]CC[CT] 1052 GGGACCT PstI 1197 CTGCAG CTGCA^G 1497 CTGCAG SfaNI 544 GATGC GCATC(5/9) 1424 GCATC SmlI 1186 CTTAAG C^T[CT][GA]AG 1264 CTTGAG TaqI 881 TCGA T^CGA 986 TCGA Tsp45I 185 GTCAC ^GT[GC]AC 364 GTGAC Tth111I 534 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1474 GACATGGTC TthHB8I 881 TCGA T^CGA 986 TCGA XmnI 165 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 891 GAAGCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BssSI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StuI StyI Tru9I TseI TspRI XhoII