(Circular) (Six-base) MAPSORT of: pFN21AE1946.seq Check: 6237 from: 1 to: 6208 pFN21AE1946 6208 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3404 3404 Size: 6208 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 2040 3529 2040 Size: 1489 4719 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2161 2462 2813 4271 5655 5923 1632 Size: 529 301 351 1458 1384 268 1917 Fragments arranged by size: 1917 1458 1384 529 351 301 268 AflII C'TTAA_G Cuts at: 820 1017 3191 820 Size: 197 2174 3837 Fragments arranged by size: 3837 2174 197 AflIII A'CryG_T Cuts at: 1203 2670 5107 6084 1203 Size: 1467 2437 977 1327 Fragments arranged by size: 2437 1467 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 3379 4937 3379 Size: 1558 4650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6208 AlwNI CAG_nnn'CTG Cuts at: 2420 3358 3951 5523 2420 Size: 938 593 1572 3105 Fragments arranged by size: 3105 1572 938 593 ApaI G_GGCC'C Cuts at: 2466 3151 2466 Size: 685 5523 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 2063 3517 3743 3895 6004 1493 Size: 570 1454 226 152 2109 1697 Fragments arranged by size: 2109 1697 1454 570 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2459 3377 3533 1870 Size: 73 516 918 156 4545 Fragments arranged by size: 4545 918 516 156 73 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2084 2240 2401 3529 Size: 325 217 627 253 44 156 161 1128 Cuts at: 3529 4984 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1128 627 325 253 217 161 156 44 BanII G_rGCy'C Cuts at: 1391 2460 2466 2478 3151 3333 3527 1391 Size: 1069 6 12 673 182 194 4072 Fragments arranged by size: 4072 1069 673 194 182 12 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2167 928 Size: 971 268 4969 Fragments arranged by size: 4969 971 268 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2814 3155 3412 Size: 107 122 71 631 605 1142 341 257 Cuts at: 3412 4819 136 Size: 1407 1525 Fragments arranged by size: 1525 1407 1142 631 605 341 257 122 107 71 BglII A'GATC_T Cuts at: 6203 6203 Size: 6208 BlpI GC'TnA_GC Cuts at: 3625 3625 Size: 6208 Bme1580I G_kGCm'C Cuts at: 1790 2089 2466 2689 3151 3408 4269 5425 1790 Size: 299 377 223 462 257 861 1156 2573 Fragments arranged by size: 2573 1156 861 462 377 299 257 223 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3939 4897 442 Size: 416 53 83 399 24 2522 958 1753 Fragments arranged by size: 2522 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2711 2957 2963 3347 4868 1239 Size: 1472 246 6 384 1521 2579 Fragments arranged by size: 2579 1521 1472 384 246 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3290 4333 5198 5496 3290 Size: 1043 865 298 4002 Fragments arranged by size: 4002 1043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2098 4871 882 Size: 617 599 2773 2219 Fragments arranged by size: 2773 2219 617 599 BsaAI yAC'GTr Cuts at: 493 1204 2671 3999 493 Size: 711 1467 1328 2702 Fragments arranged by size: 2702 1467 1328 711 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4015 4397 5032 275 Size: 53 83 186 936 2482 382 635 1451 Fragments arranged by size: 2482 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4641 5039 5313 5460 5871 4641 Size: 398 274 147 411 4978 Fragments arranged by size: 4978 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5983 Fragments arranged by size: 5983 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 1186 Size: 826 5382 BseYI C'CCAG_C Cuts at: 2426 2707 2787 2810 2874 2925 3179 3243 5411 Size: 281 80 23 64 51 254 64 2168 Cuts at: 5411 5907 2426 Size: 496 2727 Fragments arranged by size: 2727 2168 496 281 254 80 64 64 51 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2056 1163 Size: 573 320 5315 Fragments arranged by size: 5315 573 320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2818 2995 3658 4419 5447 1540 Size: 275 186 817 177 663 761 1028 2301 Fragments arranged by size: 2301 1028 817 761 663 275 186 177 BsiHKAI G_wGCw'C Cuts at: 4269 4354 5425 4269 Size: 85 1071 5052 Fragments arranged by size: 5052 1071 85 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2460 2466 2478 2689 3151 3333 Size: 399 299 371 6 12 211 462 182 Cuts at: 3333 3408 3527 4269 4354 5425 1391 Size: 75 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 462 399 371 299 211 182 119 85 75 12 6 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2998 3404 3563 844 Size: 2154 406 159 3489 Fragments arranged by size: 3489 2154 406 159 BsrBI CCG'CTC Cuts at: 1394 1823 3087 4095 1394 Size: 429 1264 1008 3507 Fragments arranged by size: 3507 1264 1008 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3311 4703 4877 65 Size: 1183 2063 1392 174 1396 Fragments arranged by size: 2063 1396 1392 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2817 2824 4852 5039 1700 Size: 110 1007 7 2028 187 2869 Fragments arranged by size: 2869 2028 1007 187 110 7 BsrGI T'GTAC_A Cuts at: 96 2532 96 Size: 2436 3772 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 2469 2513 3014 4268 5280 2469 Size: 44 501 1254 1012 3397 Fragments arranged by size: 3397 1254 1012 501 44 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6208 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstXI CCAn_nnnn'nTGG Cuts at: 3457 3457 Size: 6208 BstYI r'GATC_y Cuts at: 1972 2417 2724 2867 3538 3905 4290 4307 5759 Size: 445 307 143 671 367 385 17 1452 Cuts at: 5759 5845 5857 6203 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 671 445 385 367 346 307 143 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2649 2967 2977 3369 513 Size: 552 1584 318 10 392 3352 Fragments arranged by size: 3352 1584 552 392 318 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3244 487 Size: 683 56 2018 3451 Fragments arranged by size: 3451 2018 683 56 BtsI GCAGTG_nn' Cuts at: 3215 3286 3724 4518 4538 3215 Size: 71 438 794 20 4885 Fragments arranged by size: 4885 794 438 71 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5215 809 Size: 507 3899 1802 Fragments arranged by size: 3899 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2598 2751 Size: 54 1425 50 275 18 300 468 153 Cuts at: 2751 2815 3040 3091 3156 3655 4544 6173 8 Size: 64 225 51 65 499 889 1629 43 Fragments arranged by size: 1629 1425 889 499 468 300 275 225 153 65 64 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2815 3655 1537 Size: 275 1003 840 4090 Fragments arranged by size: 4090 1003 840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2906 3440 4138 1353 Size: 1553 534 698 3423 Fragments arranged by size: 3423 1553 698 534 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4795 5169 5315 387 Size: 1064 138 3206 374 146 1280 Fragments arranged by size: 3206 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2161 2462 2711 2813 2957 2963 3347 Size: 393 529 301 249 102 144 6 384 Cuts at: 3347 4271 4868 5655 5923 1239 Size: 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 597 529 393 384 301 268 249 144 102 6 EcoNI CCTnn'n_nnAGG Cuts at: 2693 2693 Size: 6208 EcoO109I rG'GnC_Cy Cuts at: 2154 2463 2645 3022 3058 3148 2154 Size: 309 182 377 36 90 5214 Fragments arranged by size: 5214 377 309 182 90 36 EcoRI G'AATT_C Cuts at: 2063 3517 3895 2063 Size: 1454 378 4376 Fragments arranged by size: 4376 1454 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2402 2402 Size: 6208 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 5355 1678 Size: 3677 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2831 2863 3365 3397 4849 Size: 32 135 32 1259 32 502 32 1452 Cuts at: 4849 4881 4923 4955 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1452 1259 502 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3226 3552 3798 669 Size: 988 281 1288 326 246 3079 Fragments arranged by size: 3079 1288 988 326 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KpnI G_GTAC'C Cuts at: 2044 3533 2044 Size: 1489 4719 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 3188 5322 5506 1305 Size: 1260 623 2134 184 2007 Fragments arranged by size: 2134 2007 1260 623 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2132 2600 3093 3158 10 Size: 54 1425 343 300 468 493 65 3060 Fragments arranged by size: 3060 1425 493 468 343 300 65 54 MslI CAynn'nnrTG Cuts at: 518 1166 2196 3485 4166 4525 4684 518 Size: 648 1030 1289 681 359 159 2042 Fragments arranged by size: 2042 1289 1030 681 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2762 2791 2805 2814 2929 3133 3624 4301 Size: 1156 29 14 9 115 204 491 677 Cuts at: 4301 5449 5694 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1156 1148 677 491 245 204 115 29 14 9 NaeI GCC'GGC Cuts at: 2819 2826 2819 Size: 7 6201 NcoI C'CATG_G Cuts at: 513 1065 3369 513 Size: 552 2304 3352 Fragments arranged by size: 3352 2304 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NgoMIV G'CCGG_C Cuts at: 2817 2824 2817 Size: 7 6201 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 2524 3224 3566 5111 1132 Size: 97 1295 700 342 1545 2229 Fragments arranged by size: 2229 1545 1295 700 342 97 PasI CC'CwG_GG Cuts at: 1348 2110 1348 Size: 762 5446 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6208 PfoI T'CCnGG_A Cuts at: 2389 2389 Size: 6208 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3107 3139 4277 4309 5790 5822 3107 Size: 32 1138 32 1481 32 3493 Fragments arranged by size: 3493 1481 1138 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2645 3058 2645 Size: 413 5795 PshAI GACnn'nnGTC Cuts at: 1613 2643 1613 Size: 1030 5178 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspOMI G'GGCC_C Cuts at: 2462 3147 2462 Size: 685 5523 PstI C_TGCA'G Cuts at: 830 3206 3506 3560 830 Size: 2376 300 54 3478 Fragments arranged by size: 3478 2376 300 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2791 2805 2929 2791 Size: 14 124 6070 Fragments arranged by size: 6070 124 14 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SapI GCTCTTCn'nnn_ Cuts at: 2906 2906 Size: 6208 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SfcI C'TryA_G Cuts at: 826 1045 2355 3202 3502 3556 4691 5372 5563 Size: 219 1310 847 300 54 1135 681 191 Cuts at: 5563 826 Size: 1471 Fragments arranged by size: 1471 1310 1135 847 681 300 219 191 54 SfiI GGCCn_nnn'nGGCC Cuts at: 3155 3155 Size: 6208 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 2461 3535 1872 Size: 589 1074 4545 Fragments arranged by size: 4545 1074 589 SmlI C'TyrA_G Cuts at: 820 1017 1943 3191 3269 4312 5213 5475 820 Size: 197 926 1248 78 1043 901 262 1553 Fragments arranged by size: 1553 1248 1043 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SrfI GCCC'GGGC Cuts at: 2461 2461 Size: 6208 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 2259 2859 2948 1517 Size: 742 600 89 4777 Fragments arranged by size: 4777 742 600 89 StyI C'CwwG_G Cuts at: 513 1065 2145 2478 3369 3647 513 Size: 552 1080 333 891 278 3074 Fragments arranged by size: 3074 1080 891 552 333 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 2655 3406 4235 4252 4405 4590 Size: 1255 492 583 751 829 17 153 185 Cuts at: 4590 5035 325 Size: 445 1498 Fragments arranged by size: 1498 1255 829 751 583 492 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2517 2532 4454 Size: 274 80 33 51 1443 540 15 1922 Cuts at: 4454 6166 96 Size: 1712 138 Fragments arranged by size: 1922 1712 1443 540 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6168 6200 6168 Size: 32 6176 Tth111I GACn'n_nGTC Cuts at: 1316 2542 3482 1316 Size: 1226 940 4042 Fragments arranged by size: 4042 1226 940 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2125 2125 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 2459 3533 1870 Size: 589 1074 4545 Fragments arranged by size: 4545 1074 589 XmnI GAAnn'nnTTC Cuts at: 2174 2900 4337 2174 Size: 726 1437 4045 Fragments arranged by size: 4045 1437 726 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SwaI