################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MscI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StyI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 60 CGCG CG^CG AccIII 506 TCCGGA T^CCGGA AflIII 803 ACATGT A^C[GA][CT]GT AlwNI 701 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 674 CCTCAGG CC^T[ACGT]AGG ApaI 564 GGGCCC GGGCC^C BamHI 630 GGATCC G^GATCC BbsI 586 GAAGAC GAAGAC(2/6) BglII 640 AGATCT A^GATCT BpuAI 586 GAAGAC GAAGAC(2/6) BsaI 392 GGTCTC GGTCTC(1/5) BseAI 506 TCCGGA T^CCGGA BseRI 382 GAGGAG GAGGAG(10/8) BsmAI 393 GTCTC GTCTC(1/5) BspDI 334 ATCGAT AT^CGAT BspEI 506 TCCGGA T^CCGGA BspLU11I 803 ACATGT A^CATGT BspMI 46 ACCTGC ACCTGC(4/8) BsrFI 36 GCCGGC [GA]^CCGG[CT] BsrI 533 CCAGT ACTGG(1/-1) BstEII 121 GGTGACC G^GT[ACGT]ACC BstPI 121 GGTGACC G^GT[ACGT]ACC BstUI 60 CGCG CG^CG Bsu36I 674 CCTCAGG CC^T[ACGT]AGG Cfr10I 36 GCCGGC [GA]^CCGG[CT] ClaI 334 ATCGAT AT^CGAT DsaI 251 CCGTGG C^C[GA][CT]GG EaeI 38 CGGCCA [CT]^GGCC[GA] Eco81I 674 CCTCAGG CC^T[ACGT]AGG EcoO65I 121 GGTGACC G^GT[ACGT]ACC HgaI 58 GACGC GACGC(5/10) MboII 586 GAAGA GAAGA(8/7) MroI 506 TCCGGA T^CCGGA MspA1I 164 CAGCTG C[AC]G^C[GT]G MvnI 60 CGCG CG^CG NaeI 36 GCCGGC GCC^GGC NgoMIV 36 GCCGGC G^CCGGC NspI 803 ACATGT [GA]CATG^[CT] PstI 48 CTGCAG CTGCA^G PvuII 164 CAGCTG CAG^CTG SacI 719 GAGCTC GAGCT^C SbfI 47 CCTGCAGG CCTGCA^GG SfcI 48 CTGCAG C^T[GA][CT]AG Sse8387I 47 CCTGCAGG CCTGCA^GG StuI 678 AGGCCT AGG^CCT TaqI 335 TCGA T^CGA TthHB8I 335 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 16 GTAC GT^AC 807 GTAC AspHI 656 GAGCAC G[AT]GC[AT]^C 719 GAGCTC BanII 564 GGGCCC G[GA]GC[CT]^C 719 GAGCTC BpmI 698 CTCCAG CTGGAG(16/14) 10 CTGGAG BsaWI 200 TCCGGT [AT]^CCGG[AT] 506 TCCGGA BsiHKAI 656 GAGCAC G[AT]GC[AT]^C 719 GAGCTC BstF5I 216 GGATG GGATG(2/0) 324 GGATG BstYI 630 GGATCC [GA]^GATC[CT] 640 AGATCT DraII 132 GGGTCCC [GA]G^G[ACGT]CC[CT] 364 AGGACCT DrdI 58 GACGCGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 187 GACCTGTATGTC EcoNI 607 CCTCGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 670 CCTTCCTCAGG EcoO109I 132 GGGTCCC [GA]G^G[ACGT]CC[CT] 364 AGGACCT FauI 786 CCCGC CCCGC(4/6) 4 GCGGG FokI 216 GGATG GGATG(9/13) 324 GGATG HaeII 33 AGCGCC [GA]GCGC^[CT] 703 GGCGCT HinfI 410 GAATC G^A[ACGT]TC 510 GATTC MflI 630 GGATCC [GA]^GATC[CT] 640 AGATCT PpuMI 132 GGGTCCC [GA]G^G[AT]CC[CT] 364 AGGACCT RsaI 16 GTAC GT^AC 807 GTAC SfaNI 337 GATGC GCATC(5/9) 25 GCATC TfiI 410 GAATC G^A[AT]TC 510 GATTC TspRI 378 CAGTG CA[GC]TG[ACGT][ACGT]^ 433 CAGTG XhoII 630 GGATCC [GA]^GATC[CT] 640 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I