(Circular) (Six-base) MAPSORT of: pFN21AE1766.seq Check: 24 from: 1 to: 5518 pFN21AE1766 5518 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2060 2060 Size: 5518 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3328 278 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2861 1937 Size: 924 4594 Acc65I G'GTAC_C Cuts at: 2839 2839 Size: 5518 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3581 4965 5233 1632 Size: 1949 1384 268 1917 Fragments arranged by size: 1949 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 2808 4417 5394 1203 Size: 1605 1609 977 1327 Fragments arranged by size: 1609 1605 1327 977 AgeI A'CCGG_T Cuts at: 4349 4349 Size: 5518 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 2355 1166 Size: 1189 4329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5518 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2009 2041 2009 Size: 32 5486 AlwNI CAG_nnn'CTG Cuts at: 2711 3261 4833 2711 Size: 550 1572 3396 Fragments arranged by size: 3396 1572 550 ApaI G_GGCC'C Cuts at: 2573 2573 Size: 5518 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2827 3053 3205 5314 1493 Size: 1334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 226 152 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2843 1870 Size: 73 900 4545 Fragments arranged by size: 4545 900 73 BamHI G'GATC_C Cuts at: 2635 2848 3215 2635 Size: 213 367 4938 Fragments arranged by size: 4938 367 213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2839 4294 618 Size: 325 217 627 1052 1455 1842 Fragments arranged by size: 1842 1455 1052 627 325 217 BanII G_rGCy'C Cuts at: 1391 2573 2728 2837 1391 Size: 1182 155 109 4072 Fragments arranged by size: 4072 1182 155 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2598 928 Size: 971 699 3848 Fragments arranged by size: 3848 971 699 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3709 3743 1903 Size: 34 1772 34 3678 Fragments arranged by size: 3678 1772 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 1548 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4129 136 Size: 107 122 71 631 605 2457 1525 Fragments arranged by size: 2457 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2645 5513 2645 Size: 2868 2650 BlpI GC'TnA_GC Cuts at: 2935 2935 Size: 5518 Bme1580I G_kGCm'C Cuts at: 1790 2573 2718 3579 4735 1790 Size: 783 145 861 1156 2573 Fragments arranged by size: 2573 1156 861 783 145 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5518 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3249 4207 442 Size: 416 53 83 399 24 1832 958 1753 Fragments arranged by size: 1832 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2688 4178 1239 Size: 797 652 1490 2579 Fragments arranged by size: 2579 1490 797 652 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4508 4806 3643 Size: 865 298 4355 Fragments arranged by size: 4355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2403 4181 882 Size: 617 904 1778 2219 Fragments arranged by size: 2219 1778 904 617 BsaAI yAC'GTr Cuts at: 493 1204 3309 493 Size: 711 2105 2702 Fragments arranged by size: 2702 2105 711 BsaBI GATnn'nnATC Cuts at: 5512 5512 Size: 5518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3325 3707 4342 275 Size: 53 83 186 936 1792 382 635 1451 Fragments arranged by size: 1792 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2205 2511 3951 4349 4623 4770 5181 2205 Size: 306 1440 398 274 147 411 2542 Fragments arranged by size: 2542 1440 411 398 306 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 2381 2411 1178 Size: 30 165 30 606 30 342 30 4285 Fragments arranged by size: 4285 606 342 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 1186 Size: 1216 4302 BseYI C'CCAG_C Cuts at: 2171 4721 5217 2171 Size: 2550 496 2472 Fragments arranged by size: 2550 2472 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4945 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2968 3729 4757 1540 Size: 275 186 967 761 1028 2301 Fragments arranged by size: 2301 1028 967 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2665 2728 3579 3664 4735 2665 Size: 63 851 85 1071 3448 Fragments arranged by size: 3448 1071 851 85 63 BsmI GAATG_Cn' Cuts at: 3029 3122 3029 Size: 93 5425 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2573 2665 2718 2728 2837 3579 3664 Size: 399 783 92 53 10 109 742 85 Cuts at: 3664 4735 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 783 742 399 109 92 85 53 10 BspEI T'CCGG_A Cuts at: 2511 5181 2511 Size: 2670 2848 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2060 2873 844 Size: 1216 813 3489 Fragments arranged by size: 3489 1216 813 BsrBI CCG'CTC Cuts at: 1394 1823 3405 1394 Size: 429 1582 3507 Fragments arranged by size: 3507 1582 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4013 4187 65 Size: 1183 2765 174 1396 Fragments arranged by size: 2765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2041 4162 4349 1700 Size: 110 231 2121 187 2869 Fragments arranged by size: 2869 2121 231 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5518 BssSI C'ACGA_G Cuts at: 3578 4590 3578 Size: 1012 4506 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5518 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 2126 1184 Size: 942 4576 BstXI CCAn_nnnn'nTGG Cuts at: 2175 2257 2500 2175 Size: 82 243 5193 Fragments arranged by size: 5193 243 82 BstYI r'GATC_y Cuts at: 1972 2635 2645 2848 3215 3600 3617 5069 5155 Size: 663 10 203 367 385 17 1452 86 Cuts at: 5155 5167 5513 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 663 385 367 346 203 86 17 12 10 Bsu36I CC'TnA_GG Cuts at: 2680 2680 Size: 5518 BtgI C'CryG_G Cuts at: 513 1065 2004 2256 513 Size: 552 939 252 3775 Fragments arranged by size: 3775 939 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4779 Fragments arranged by size: 4779 683 56 BtsI GCAGTG_nn' Cuts at: 2444 3034 3828 3848 2444 Size: 590 794 20 4114 Fragments arranged by size: 4114 794 590 20 ClaI AT'CG_AT Cuts at: 1554 2340 1554 Size: 786 4732 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2069 2198 4525 809 Size: 507 753 129 2327 1802 Fragments arranged by size: 2327 1802 753 507 129 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2965 3854 Size: 54 1425 50 275 18 213 922 889 Cuts at: 3854 5483 8 Size: 1629 43 Fragments arranged by size: 1629 1425 922 889 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2965 1537 Size: 275 1153 4090 Fragments arranged by size: 4090 1153 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3448 1353 Size: 2095 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2726 2726 Size: 5518 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2688 3581 4178 4965 5233 1239 Size: 393 404 652 893 597 787 268 1524 Fragments arranged by size: 1524 893 787 652 597 404 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2616 2679 2616 Size: 63 5455 EcoO109I rG'GnC_Cy Cuts at: 2138 2370 2138 Size: 232 5286 EcoRI G'AATT_C Cuts at: 2827 3205 2827 Size: 378 5140 FspI TGC'GCA Cuts at: 4024 4024 Size: 5518 HaeII r_GCGC'y Cuts at: 1678 2042 2712 4665 1678 Size: 364 670 1953 2531 Fragments arranged by size: 2531 1953 670 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2381 2413 2620 Size: 32 135 32 566 32 211 32 207 Cuts at: 2620 2652 2663 2695 4159 4191 4233 4265 1373 Size: 32 11 32 1464 32 42 32 2626 Fragments arranged by size: 2626 1464 566 211 207 135 42 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5518 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KpnI G_GTAC'C Cuts at: 2843 2843 Size: 5518 MfeI C'AATT_G Cuts at: 3117 3117 Size: 5518 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4632 4816 1305 Size: 3327 184 2007 Fragments arranged by size: 3327 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3696 Fragments arranged by size: 3696 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2355 2630 3476 3835 3994 518 Size: 648 1089 100 275 846 359 159 2042 Fragments arranged by size: 2042 1089 846 648 359 275 159 100 MspA1I CmG'CkG Cuts at: 1606 2171 2934 3611 4759 5004 1606 Size: 565 763 677 1148 245 2120 Fragments arranged by size: 2120 1148 763 677 565 245 NaeI GCC'GGC Cuts at: 2043 2043 Size: 5518 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4027 Fragments arranged by size: 4027 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NgoMIV G'CCGG_C Cuts at: 2041 2041 Size: 5518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2965 2965 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2812 2876 4421 1132 Size: 97 1583 64 1545 2229 Fragments arranged by size: 2229 1583 1545 97 64 PasI CC'CwG_GG Cuts at: 1348 2566 1348 Size: 1218 4300 PciI A'CATG_T Cuts at: 2808 4417 2808 Size: 1609 3909 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5518 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2009 2041 3587 3619 5100 5132 2009 Size: 32 1546 32 1481 32 2395 Fragments arranged by size: 2395 1546 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2138 2370 2138 Size: 232 5286 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5518 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PspOMI G'GGCC_C Cuts at: 2569 2569 Size: 5518 PstI C_TGCA'G Cuts at: 830 2057 2870 830 Size: 1227 813 3478 Fragments arranged by size: 3478 1227 813 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 PvuII CAG'CTG Cuts at: 2171 2171 Size: 5518 SacI G_AGCT'C Cuts at: 2728 2728 Size: 5518 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SanDI GG'GwC_CC Cuts at: 2138 2138 Size: 5518 SbfI CC_TGCA'GG Cuts at: 2057 2870 2057 Size: 813 4705 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SexAI A'CCwGG_T Cuts at: 2814 2814 Size: 5518 SfcI C'TryA_G Cuts at: 826 1045 2053 2866 4001 4682 4873 826 Size: 219 1008 813 1135 681 191 1471 Fragments arranged by size: 1471 1135 1008 813 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5518 SmaI CCC'GGG Cuts at: 1872 2845 1872 Size: 973 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3622 4523 4785 820 Size: 197 926 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2876 1132 Size: 1744 3774 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 2685 1517 Size: 1168 4350 StyI C'CwwG_G Cuts at: 513 1065 2004 2957 513 Size: 552 939 953 3074 Fragments arranged by size: 3074 953 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2108 2163 2345 2385 2544 3545 3562 Size: 1255 528 55 182 40 159 1001 17 Cuts at: 3562 3715 3900 4345 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1001 528 445 185 182 159 153 55 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3764 5476 96 Size: 274 80 33 51 1443 1787 1712 138 Fragments arranged by size: 1787 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5478 5510 5478 Size: 32 5486 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5518 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2843 1870 Size: 973 4545 XmnI GAAnn'nnTTC Cuts at: 3647 3647 Size: 5518 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3326 276 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI