Restriction map for pFN21AE1766
 (Linear) (Six-base) MAPSORT of: KIEE1766.seq  Check: 9282  from: 1  to: 813



KIEE1766.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      55     813 
   Size:         55     758
 
AflIII A'CryG_T

Cuts at:      0     803     813 
   Size:        803      10
 
AleI CACnn'nnGTG

Cuts at:      0     350     813 
   Size:        350     463
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       4      36     813 
   Size:          4      32     777

  Fragments arranged by size: 

                 777      32       4
 
AlwNI CAG_nnn'CTG

Cuts at:      0     706     813 
   Size:        706     107
 
ApaI G_GGCC'C

Cuts at:      0     568     813 
   Size:        568     245
 
BamHI G'GATC_C

Cuts at:      0     630     813 
   Size:        630     183
 
BanII G_rGCy'C

Cuts at:      0     568     723     813 
   Size:        568     155      90

  Fragments arranged by size: 

                 568     155      90
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     593     813 
   Size:        593     220
 
BglII A'GATC_T

Cuts at:      0     640     813 
   Size:        640     173
 
Bme1580I G_kGCm'C

Cuts at:      0     568     713     813 
   Size:        568     145     100

  Fragments arranged by size: 

                 568     145     100
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     683     813 
   Size:         31     652     130

  Fragments arranged by size: 

                 652     130      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     398     813 
   Size:        398     415
 
BsaWI w'CCGG_w

Cuts at:      0     200     506     813 
   Size:        200     306     307

  Fragments arranged by size: 

                 307     306     200
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       4      34     376     406     813 
   Size:          4      30     342      30     407

  Fragments arranged by size: 

                 407     342      30      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     397     813 
   Size:        397     416
 
BseYI C'CCAG_C

Cuts at:      0     166     813 
   Size:        166     647
 
BsiHKAI G_wGCw'C

Cuts at:      0     660     723     813 
   Size:        660      63      90

  Fragments arranged by size: 

                 660      90      63
 
Bsp1286I G_dGCh'C

Cuts at:      0     568     660     713     723     813 
   Size:        568      92      53      10      90

  Fragments arranged by size: 

                 568      92      90      53      10
 
BspEI T'CCGG_A

Cuts at:      0     506     813 
   Size:        506     307
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      55     813 
   Size:         55     758
 
BsrFI r'CCGG_y

Cuts at:      0      36     813 
   Size:         36     777
 
BstEII G'GTnAC_C

Cuts at:      0     121     813 
   Size:        121     692
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     170     252     495     813 
   Size:        170      82     243     318

  Fragments arranged by size: 

                 318     243     170      82
 
BstYI r'GATC_y

Cuts at:      0     630     640     813 
   Size:        630      10     173

  Fragments arranged by size: 

                 630     173      10
 
Bsu36I CC'TnA_GG

Cuts at:      0     675     813 
   Size:        675     138
 
BtgI C'CryG_G

Cuts at:      0     251     813 
   Size:        251     562
 
BtsI GCAGTG_nn'

Cuts at:      0     439     813 
   Size:        439     374
 
ClaI AT'CG_AT

Cuts at:      0     335     813 
   Size:        335     478
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      64     193     813 
   Size:         64     129     620

  Fragments arranged by size: 

                 620     129      64
 
EaeI y'GGCC_r

Cuts at:      0      38     813 
   Size:         38     775
 
EcoICRI GAG'CTC

Cuts at:      0     721     813 
   Size:        721      92
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     683     813 
   Size:         31     652     130

  Fragments arranged by size: 

                 652     130      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     611     674     813 
   Size:        611      63     139

  Fragments arranged by size: 

                 611     139      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     133     365     813 
   Size:        133     232     448

  Fragments arranged by size: 

                 448     232     133
 
HaeII r_GCGC'y

Cuts at:      0      37     707     813 
   Size:         37     670     106

  Fragments arranged by size: 

                 670     106      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     133     165     376     408     615     647     658     690 
   Size:        133      32     211      32     207      32      11      32

Cuts at:    690     813 
   Size:        123

  Fragments arranged by size: 

                 211     207     133     123      32      32      32      32
                  11
 
MslI CAynn'nnrTG

Cuts at:      0     250     350     625     813 
   Size:        250     100     275     188

  Fragments arranged by size: 

                 275     250     188     100
 
MspA1I CmG'CkG

Cuts at:      0     166     813 
   Size:        166     647
 
NaeI GCC'GGC

Cuts at:      0      38     813 
   Size:         38     775
 
NgoMIV G'CCGG_C

Cuts at:      0      36     813 
   Size:         36     777
 
NspI r_CATG'y

Cuts at:      0     807     813 
   Size:        807       6
 
PasI CC'CwG_GG

Cuts at:      0     561     813 
   Size:        561     252
 
PciI A'CATG_T

Cuts at:      0     803     813 
   Size:        803      10
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     813 
   Size:          4      32     777

  Fragments arranged by size: 

                 777      32       4
 
PpuMI rG'GwC_Cy

Cuts at:      0     133     365     813 
   Size:        133     232     448

  Fragments arranged by size: 

                 448     232     133
 
PspOMI G'GGCC_C

Cuts at:      0     564     813 
   Size:        564     249
 
PstI C_TGCA'G

Cuts at:      0      52     813 
   Size:         52     761
 
PvuII CAG'CTG

Cuts at:      0     166     813 
   Size:        166     647
 
SacI G_AGCT'C

Cuts at:      0     723     813 
   Size:        723      90
 
SanDI GG'GwC_CC

Cuts at:      0     133     813 
   Size:        133     680
 
SbfI CC_TGCA'GG

Cuts at:      0      52     813 
   Size:         52     761
 
SfcI C'TryA_G

Cuts at:      0      48     813 
   Size:         48     765
 
StuI AGG'CCT

Cuts at:      0     680     813 
   Size:        680     133
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     103     158     340     380     539     813 
   Size:        103      55     182      40     159     274

  Fragments arranged by size: 

                 274     182     159     103      55      40
 

 Enzymes that do cut:

     AarI   AflIII     AleI     AloI    AlwNI     ApaI    BamHI    BanII
     BbsI    BglII Bme1580I     BpmI     BsaI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrFI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI     ClaI     DrdI     EaeI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     MslI   MspA1I     NaeI
   NgoMIV     NspI     PasI     PciI     PpiI    PpuMI   PspOMI     PstI
    PvuII     SacI    SanDI     SbfI     SfcI     StuI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI  BstZ17I    BtgZI    CspCI     DraI   DraIII     EagI     EarI
     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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