################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 78 GGCGTC G[GA]^CG[CT]C AflIII 136 ACGTGT A^C[GA][CT]GT BamHI 98 GGATCC G^GATCC BanI 55 GGCACC G^G[CT][GA]CC BanII 412 GAGCCC G[GA]GC[CT]^C BclI 2 TGATCA T^GATCA BmyI 412 GAGCCC G[AGT]GC[ACT]^C BsaHI 78 GGCGTC G[GA]^CG[CT]C BsgI 490 GTGCAG GTGCAG(16/14) BsmAI 286 GAGAC GTCTC(1/5) Bsp1286I 412 GAGCCC G[AGT]GC[ACT]^C BspMI 125 ACCTGC ACCTGC(4/8) BsrFI 467 ACCGGC [GA]^CCGG[CT] BssKI 249 CCAGG ^CC[ACGT]GG BstEII 253 GGTTACC G^GT[ACGT]ACC BstF5I 339 CATCC GGATG(2/0) BstNI 249 CCAGG CC^[AT]GG BstPI 253 GGTTACC G^GT[ACGT]ACC Cfr10I 467 ACCGGC [GA]^CCGG[CT] EarI 403 CTCTTC CTCTTC(1/4) EcoNI 342 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 253 GGTTACC G^GT[ACGT]ACC EcoRII 249 CCAGG ^CC[AT]GG EcoRV 142 GATATC GAT^ATC FbaI 2 TGATCA T^GATCA FokI 339 CATCC GGATG(9/13) HapII 468 CCGG C^CGG HgaI 79 GCGTC GACGC(5/10) Hin1I 78 GGCGTC G[GA]^CG[CT]C HinfI 43 GAGTC G^A[ACGT]TC HpaII 468 CCGG C^CGG Ksp632I 403 CTCTTC CTCTTC(1/4) MaeIII 254 GTTAC ^GT[ACGT]AC MseI 239 TTAA T^TAA MspA1I 91 CAGCTG C[AC]G^C[GT]G MspI 468 CCGG C^CGG MvaI 249 CCAGG CC^[AT]GG PaeR7I 316 CTCGAG C^TCGAG PleI 43 GAGTC GAGTC(4/5) PshAI 288 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 91 CAGCTG CAG^CTG ScrFI 249 CCAGG CC^[ACGT]GG SmlI 316 CTCGAG C^T[CT][GA]AG TaqI 317 TCGA T^CGA Tru9I 239 TTAA T^TAA Tsp509I 384 AATT ^AATT TspRI 431 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 317 TCGA T^CGA XhoI 316 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Aor51HI 494 AGCGCT AGC^GCT 536 AGCGCT AvaI 316 CTCGAG C^[CT]CG[GA]G 421 CCCGAG AvaII 426 GGACC G^G[AT]CC 453 GGTCC BsiYI 196 CCTGATGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 342 CCTCAGAGAGG BslI 196 CCTGATGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 342 CCTCAGAGAGG BsoBI 316 CTCGAG C^[CT]CG[GA]G 421 CCCGAG BsrI 353 ACTGG ACTGG(1/-1) 361 CCAGT BstAPI 323 GCAACGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 483 GCAGAACGTGC BstYI 98 GGATCC [GA]^GATC[CT] 156 AGATCC Cac8I 49 GCGGGC GC[ACGT]^[ACGT]GC 392 GCCTGC DdeI 153 CTCAG C^T[ACGT]AG 343 CTCAG DraII 425 AGGACCC [GA]G^G[ACGT]CC[CT] 452 AGGTCCT Eco47III 494 AGCGCT AGC^GCT 536 AGCGCT EcoO109I 425 AGGACCC [GA]G^G[ACGT]CC[CT] 452 AGGTCCT EcoT14I 59 CCAAGG C^C[AT][AT]GG 272 CCAAGG HaeII 494 AGCGCT [GA]GCGC^[CT] 536 AGCGCT HphI 276 GGTGA GGTGA(8/7) 300 TCACC MboII 30 GAAGA GAAGA(8/7) 404 TCTTC MflI 98 GGATCC [GA]^GATC[CT] 156 AGATCC NlaIII 262 CATG CATG^ 501 CATG PpuMI 425 AGGACCC [GA]G^G[AT]CC[CT] 452 AGGTCCT PstI 394 CTGCAG CTGCA^G 457 CTGCAG SfcI 394 CTGCAG C^T[GA][CT]AG 457 CTGCAG StyI 59 CCAAGG C^C[AT][AT]GG 272 CCAAGG XhoII 98 GGATCC [GA]^GATC[CT] 156 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI AlwI BbvI BsaJI BseRI CfoI Cfr13I DpnII FauI Fnu4HI HaeIII HhaI HinP1I ItaI MaeII MboI MnlI MwoI NdeII NlaIV Sau3AI Sau96I TseI