Restriction map for
pFN21AE1689
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DpnII DraI DraII DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI HphI KpnI KspI MaeI
MaeIII MamI MboI MfeI MflI
MluI MroI MseI MslI MunI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI Sau3AI ScaI
SexAI SfiI SfuI SmaI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI Tru9I Tsp45I Tth111I
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 158 CGCG CG^CG
AcsI 187 AAATTC [GA]^AATT[CT]
AhdI 298 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 187 AAATTC [GA]^AATT[CT]
AspEI 298 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 319 CACGTG CAC^GTG
BlpI 33 GCTGAGC GC^T[ACGT]AGC
BpmI 364 CTGGAG CTGGAG(16/14)
Bpu1102I 33 GCTGAGC GC^T[ACGT]AGC
BsaAI 319 CACGTG [CT]AC^GT[GA]
BseRI 492 CTCCTC GAGGAG(10/8)
BstAPI 324 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 528 CATCC GGATG(2/0)
BstUI 158 CGCG CG^CG
CelII 33 GCTGAGC GC^T[ACGT]AGC
Eam1105I 298 GACCTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 348 CTCTTC CTCTTC(1/4)
FokI 528 CATCC GGATG(9/13)
Ksp632I 348 CTCTTC CTCTTC(1/4)
MaeII 320 ACGT A^CGT
MvnI 158 CGCG CG^CG
NlaIII 237 CATG CATG^
PaeR7I 86 CTCGAG C^TCGAG
PleI 588 GACTC GAGTC(4/5)
PmaCI 319 CACGTG CAC^GTG
PmlI 319 CACGTG CAC^GTG
SacI 257 GAGCTC GAGCT^C
SapI 347 GCTCTTC GCTCTTC(1/4)
SbfI 384 CCTGCAGG CCTGCA^GG
SfaNI 509 GCATC GCATC(5/9)
SgrAI 467 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 86 CTCGAG C^T[CT][GA]AG
Sse8387I 384 CCTGCAGG CCTGCA^GG
TaqI 87 TCGA T^CGA
TfiI 217 GATTC G^A[AT]TC
Tsp509I 188 AATT ^AATT
TthHB8I 87 TCGA T^CGA
XhoI 86 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 471 GGCGCC G[GA]^CG[CT]C
483 GGCGCC
AlwNI 325 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
421 CAGCTCCTG
AspHI 127 GTGCTC G[AT]GC[AT]^C
257 GAGCTC
BalI 43 TGGCCA TGG^CCA
233 TGGCCA
BanII 257 GAGCTC G[GA]GC[CT]^C
367 GAGCCC
BbeI 471 GGCGCC GGCGC^C
483 GGCGCC
BbsI 357 GAAGAC GAAGAC(2/6)
585 GAAGAC
BbvCI 494 CCTCAGC CCTCAGC(-5/-2)
177 GCTGAGG
BcnI 336 CCGGG CC^[GC]GG
459 CCCGG
BpuAI 357 GAAGAC GAAGAC(2/6)
585 GAAGAC
BsaHI 471 GGCGCC G[GA]^CG[CT]C
483 GGCGCC
BsiHKAI 127 GTGCTC G[AT]GC[AT]^C
257 GAGCTC
BspMI 311 ACCTGC ACCTGC(4/8)
340 GCAGGT
BsrBI 138 CCGCTC CCGCTC(-3/-3)
293 GAGCGG
EaeI 43 TGGCCA [CT]^GGCC[GA]
233 TGGCCA
HaeII 471 GGCGCC [GA]GCGC^[CT]
483 GGCGCC
Hin1I 471 GGCGCC G[GA]^CG[CT]C
483 GGCGCC
HinfI 217 GATTC G^A[ACGT]TC
588 GACTC
KasI 471 GGCGCC G^GCGCC
483 GGCGCC
MluNI 43 TGGCCA TGG^CCA
233 TGGCCA
MscI 43 TGGCCA TGG^CCA
233 TGGCCA
NaeI 413 GCCGGC GCC^GGC
468 GCCGGC
NarI 471 GGCGCC GG^CGCC
483 GGCGCC
NciI 336 CCGGG CC^[GC]GG
459 CCCGG
NgoMIV 413 GCCGGC G^CCGGC
468 GCCGGC
PflMI 70 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
207 CCACAACCTGG
PstI 385 CTGCAG CTGCA^G
418 CTGCAG
SfoI 471 GGCGCC GGC^GCC
483 GGCGCC
TspRI 251 CACTG CA[GC]TG[ACGT][ACGT]^
325 CAGTG
Van91I 70 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
207 CCACAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AvaI AvaII BanI
BbvI BmyI BsaJI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MwoI NlaIV PvuII
Sau96I ScrFI SfcI TseI