(Circular) (Six-base) MAPSORT of: pFN21AE1689.seq Check: 2967 from: 1 to: 5302 pFN21AE1689 5302 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2336 2336 Size: 5302 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3112 278 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2645 1937 Size: 708 4594 Acc65I G'GTAC_C Cuts at: 2623 2623 Size: 5302 AclI AA'CG_TT Cuts at: 1563 3429 3802 1563 Size: 1866 373 3063 Fragments arranged by size: 3063 1866 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2381 2609 3365 4749 5017 1632 Size: 749 228 756 1384 268 1917 Fragments arranged by size: 1917 1384 756 749 268 228 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5105 AflIII A'CryG_T Cuts at: 1203 4201 5178 1203 Size: 2998 977 1327 Fragments arranged by size: 2998 1327 977 AgeI A'CCGG_T Cuts at: 4133 4133 Size: 5302 AhdI GACnn_n'nnGTC Cuts at: 2308 4031 2308 Size: 1723 3579 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2190 1184 Size: 1006 4296 AlwNI CAG_nnn'CTG Cuts at: 2335 2431 3045 4617 2335 Size: 96 614 1572 3020 Fragments arranged by size: 3020 1572 614 96 ApaLI G'TGCA_C Cuts at: 3359 4515 3359 Size: 1156 4146 ApoI r'AATT_y Cuts at: 1493 2192 2611 2837 2989 5098 1493 Size: 699 419 226 152 2109 1697 Fragments arranged by size: 2109 1697 699 419 226 152 AseI AT'TA_AT Cuts at: 160 1032 3856 160 Size: 872 2824 1606 Fragments arranged by size: 2824 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 AvaI C'yCGr_G Cuts at: 1870 1943 2091 2109 2375 2627 1870 Size: 73 148 18 266 252 4545 Fragments arranged by size: 4545 266 252 148 73 18 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2046 2079 2046 Size: 33 5269 BamHI G'GATC_C Cuts at: 2632 2999 2632 Size: 367 4935 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2384 2476 2488 2623 4078 Size: 325 217 627 597 92 12 135 1455 Cuts at: 4078 618 Size: 1842 Fragments arranged by size: 1842 1455 627 597 325 217 135 92 12 BanII G_rGCy'C Cuts at: 1391 2266 2376 2621 1391 Size: 875 110 245 4072 Fragments arranged by size: 4072 875 245 110 BbeI G_GCGC'C Cuts at: 2480 2492 2480 Size: 12 5290 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2369 2597 928 Size: 971 470 228 3633 Fragments arranged by size: 3633 971 470 228 BbvCI CC'TCA_GC Cuts at: 2183 2500 2183 Size: 317 4985 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3493 3527 1903 Size: 34 1556 34 3678 Fragments arranged by size: 3678 1556 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3194 4404 3194 Size: 1210 4092 BclI T'GATC_A Cuts at: 1548 1548 Size: 5302 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3913 136 Size: 107 122 71 631 605 2241 1525 Fragments arranged by size: 2241 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5297 5297 Size: 5302 BlpI GC'TnA_GC Cuts at: 2039 2719 2039 Size: 680 4622 Bme1580I G_kGCm'C Cuts at: 1790 2257 2387 3363 4519 1790 Size: 467 130 976 1156 2573 Fragments arranged by size: 2573 1156 976 467 130 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5302 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2267 2572 3033 Size: 416 53 83 399 24 850 305 461 Cuts at: 3033 3991 442 Size: 958 1753 Fragments arranged by size: 1753 958 850 461 416 399 305 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5302 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 3962 1239 Size: 1151 1572 2579 Fragments arranged by size: 2579 1572 1151 Bpu10I CC'TnA_GC Cuts at: 2183 2500 2183 Size: 317 4985 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3427 4292 4590 3427 Size: 865 298 4139 Fragments arranged by size: 4139 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3965 882 Size: 617 2466 2219 Fragments arranged by size: 2466 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2326 3093 493 Size: 711 1122 767 2702 Fragments arranged by size: 2702 1122 767 711 BsaBI GATnn'nnATC Cuts at: 5296 5296 Size: 5302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2477 2489 3109 3491 Size: 53 83 186 936 944 12 620 382 Cuts at: 3491 4126 275 Size: 635 1451 Fragments arranged by size: 1451 944 936 635 620 382 186 83 53 12 BsaWI w'CCGG_w Cuts at: 3735 4133 4407 4554 4965 3735 Size: 398 274 147 411 4072 Fragments arranged by size: 4072 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5077 Fragments arranged by size: 5077 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2488 1186 Size: 1302 4000 BseYI C'CCAG_C Cuts at: 2197 2380 2454 4505 5001 2197 Size: 183 74 2051 496 2498 Fragments arranged by size: 2498 2051 496 183 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2160 2268 2348 2531 1163 Size: 573 424 108 80 183 3934 Fragments arranged by size: 3934 573 424 183 108 80 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2752 3513 4541 1540 Size: 275 186 751 761 1028 2301 Fragments arranged by size: 2301 1028 761 751 275 186 BsiHKAI G_wGCw'C Cuts at: 2136 2266 3363 3448 4519 2136 Size: 130 1097 85 1071 2919 Fragments arranged by size: 2919 1097 1071 130 85 BsmI GAATG_Cn' Cuts at: 2813 2906 2813 Size: 93 5209 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2257 2266 2376 2387 2621 3363 Size: 399 346 121 9 110 11 234 742 Cuts at: 3363 3448 4519 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 346 234 121 110 85 11 9 BspEI T'CCGG_A Cuts at: 4965 4965 Size: 5302 BspHI T'CATG_A Cuts at: 3191 4921 3191 Size: 1730 3572 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2325 2336 2657 844 Size: 1481 11 321 3489 Fragments arranged by size: 3489 1481 321 11 BsrBI CCG'CTC Cuts at: 1394 1823 2145 2300 3189 1394 Size: 429 322 155 889 3507 Fragments arranged by size: 3507 889 429 322 155 BsrDI GCAATG_nn' Cuts at: 65 1248 3797 3971 65 Size: 1183 2549 174 1396 Fragments arranged by size: 2549 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2406 2418 2473 3946 4133 1700 Size: 110 596 12 55 1473 187 2869 Fragments arranged by size: 2869 1473 596 187 110 55 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5302 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5302 BssSI C'ACGA_G Cuts at: 3362 4374 3362 Size: 1012 4290 BstAPI GCAn_nnn'nTGC Cuts at: 794 2335 794 Size: 1541 3761 BstBI TT'CG_AA Cuts at: 4106 4106 Size: 5302 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5302 BstYI r'GATC_y Cuts at: 1972 2632 2999 3384 3401 4853 4939 4951 5297 Size: 660 367 385 17 1452 86 12 346 Cuts at: 5297 1972 Size: 1977 Fragments arranged by size: 1977 1452 660 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4750 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4563 Fragments arranged by size: 4563 683 56 BtsI GCAGTG_nn' Cuts at: 2336 2818 3612 3632 2336 Size: 482 794 20 4006 Fragments arranged by size: 4006 794 482 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5302 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5267 DraI TTT'AAA Cuts at: 2606 2606 Size: 5302 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4309 809 Size: 507 2993 1802 Fragments arranged by size: 2993 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2048 2238 2749 Size: 54 1425 50 275 18 218 190 511 Cuts at: 2749 3638 5267 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 511 275 218 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2749 1537 Size: 275 937 4090 Fragments arranged by size: 4090 937 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2359 3232 1353 Size: 1006 873 3423 Fragments arranged by size: 3423 1006 873 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2171 3889 4263 4409 387 Size: 1064 138 582 1718 374 146 1280 Fragments arranged by size: 1718 1280 1064 582 374 146 138 EcoICRI GAG'CTC Cuts at: 2264 2264 Size: 5302 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 2390 2609 3365 3962 4749 5017 Size: 393 749 9 219 756 597 787 268 Cuts at: 5017 1239 Size: 1524 Fragments arranged by size: 1524 787 756 749 597 393 268 219 9 EcoRI G'AATT_C Cuts at: 2611 2989 2611 Size: 378 4924 FspI TGC'GCA Cuts at: 3808 3808 Size: 5302 HaeII r_GCGC'y Cuts at: 1678 2480 2492 4449 1678 Size: 802 12 1957 2531 Fragments arranged by size: 2531 1957 802 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2131 2163 2294 2326 3943 Size: 32 135 32 559 32 131 32 1617 Cuts at: 3943 3975 4017 4049 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1617 559 135 131 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2646 2892 669 Size: 988 281 708 246 3079 Fragments arranged by size: 3079 988 708 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5302 HpaI GTT'AAC Cuts at: 2892 2892 Size: 5302 KasI G'GCGC_C Cuts at: 2476 2488 2476 Size: 12 5290 KpnI G_GTAC'C Cuts at: 2627 2627 Size: 5302 MfeI C'AATT_G Cuts at: 2901 2901 Size: 5302 MluI A'CGCG_T Cuts at: 5178 5178 Size: 5302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4416 4600 1305 Size: 3111 184 2007 Fragments arranged by size: 3111 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2050 2240 10 Size: 54 1425 343 218 190 3072 Fragments arranged by size: 3072 1425 343 218 190 54 MslI CAynn'nnrTG Cuts at: 518 1166 3260 3619 3778 518 Size: 648 2094 359 159 2042 Fragments arranged by size: 2094 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2060 2170 2182 2201 2485 2718 3395 4543 Size: 454 110 12 19 284 233 677 1148 Cuts at: 4543 4788 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 454 284 245 233 110 19 12 NaeI GCC'GGC Cuts at: 2420 2475 2420 Size: 55 5247 NarI GG'CG_CC Cuts at: 2477 2489 2477 Size: 12 5290 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4750 NdeI CA'TA_TG Cuts at: 387 387 Size: 5302 NgoMIV G'CCGG_C Cuts at: 2418 2473 2418 Size: 55 5247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5302 NotI GC'GGCC_GC Cuts at: 2749 2749 Size: 5302 NspI r_CATG'y Cuts at: 1132 1229 2660 4205 1132 Size: 97 1431 1545 2229 Fragments arranged by size: 2229 1545 1431 97 PasI CC'CwG_GG Cuts at: 1348 2069 1348 Size: 721 4581 PciI A'CATG_T Cuts at: 4201 4201 Size: 5302 PflMI CCAn_nnn'nTGG Cuts at: 1108 2081 2218 1108 Size: 973 137 4192 Fragments arranged by size: 4192 973 137 PmeI GTTT'AAAC Cuts at: 2606 2606 Size: 5302 PmlI CAC'GTG Cuts at: 2326 2326 Size: 5302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3371 3403 4884 4916 3371 Size: 32 1481 32 3757 Fragments arranged by size: 3757 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5302 PsiI TTA'TAA Cuts at: 2872 2872 Size: 5302 PspXI vC'TCGA_Gb Cuts at: 2091 2091 Size: 5302 PstI C_TGCA'G Cuts at: 830 2394 2427 2654 830 Size: 1564 33 227 3478 Fragments arranged by size: 3478 1564 227 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5302 PvuII CAG'CTG Cuts at: 2060 2170 2182 2201 2060 Size: 110 12 19 5161 Fragments arranged by size: 5161 110 19 12 SacI G_AGCT'C Cuts at: 2266 2266 Size: 5302 SalI G'TCGA_C Cuts at: 1936 2644 1936 Size: 708 4594 SapI GCTCTTCn'nnn_ Cuts at: 2359 2359 Size: 5302 SbfI CC_TGCA'GG Cuts at: 2394 2654 2394 Size: 260 5042 ScaI AGT'ACT Cuts at: 3550 5262 3550 Size: 1712 3590 SfcI C'TryA_G Cuts at: 826 1045 2267 2390 2423 2650 3785 4466 4657 Size: 219 1222 123 33 227 1135 681 191 Cuts at: 4657 826 Size: 1471 Fragments arranged by size: 1471 1222 1135 681 227 219 191 123 33 SfoI GGC'GCC Cuts at: 2478 2490 2478 Size: 12 5290 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 SgrAI Cr'CCGG_yG Cuts at: 1700 2473 1700 Size: 773 4529 SmaI CCC'GGG Cuts at: 1872 2629 1872 Size: 757 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2091 3406 4307 4569 820 Size: 197 926 148 1315 901 262 1553 Fragments arranged by size: 1553 1315 926 901 262 197 148 SnaBI TAC'GTA Cuts at: 493 3093 493 Size: 2600 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5302 SphI G_CATG'C Cuts at: 1132 2660 1132 Size: 1528 3774 SspI AAT'ATT Cuts at: 5 52 3226 5 Size: 47 3174 2081 Fragments arranged by size: 3174 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5302 StyI C'CwwG_G Cuts at: 513 1065 2741 513 Size: 552 1676 3074 Fragments arranged by size: 3074 1676 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3329 3346 3499 3684 4129 325 Size: 1255 1749 17 153 185 445 1498 Fragments arranged by size: 1749 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3548 5260 96 Size: 274 80 33 51 1443 1571 1712 138 Fragments arranged by size: 1712 1571 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5262 5294 5262 Size: 32 5270 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5302 XbaI T'CTAG_A Cuts at: 2638 2638 Size: 5302 XhoI C'TCGA_G Cuts at: 1943 2091 1943 Size: 148 5154 XmaI C'CCGG_G Cuts at: 1870 2627 1870 Size: 757 4545 XmnI GAAnn'nnTTC Cuts at: 3431 3431 Size: 5302 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3110 276 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI