Restriction map for
pFN21AE1689
(Linear) (Six-base) MAPSORT of: KIEE1689.seq Check: 6986 from: 1 to: 597
KIEE1689.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 331 597
Size: 331 266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 597
Size: 376 221
AhdI GACnn_n'nnGTC
Cuts at: 0 303 597
Size: 303 294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 185 597
Size: 185 412
AlwNI CAG_nnn'CTG
Cuts at: 0 330 426 597
Size: 330 96 171
Fragments arranged by size:
330 171 96
ApoI r'AATT_y
Cuts at: 0 187 597
Size: 187 410
AvaI C'yCGr_G
Cuts at: 0 86 104 370 597
Size: 86 18 266 227
Fragments arranged by size:
266 227 86 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 41 74 597
Size: 41 33 523
Fragments arranged by size:
523 41 33
BanI G'GyrC_C
Cuts at: 0 379 471 483 597
Size: 379 92 12 114
Fragments arranged by size:
379 114 92 12
BanII G_rGCy'C
Cuts at: 0 261 371 597
Size: 261 110 226
Fragments arranged by size:
261 226 110
BbeI G_GCGC'C
Cuts at: 0 475 487 597
Size: 475 12 110
Fragments arranged by size:
475 110 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 364 592 597
Size: 364 228 5
Fragments arranged by size:
364 228 5
BbvCI CC'TCA_GC
Cuts at: 0 178 495 597
Size: 178 317 102
Fragments arranged by size:
317 178 102
BlpI GC'TnA_GC
Cuts at: 0 34 597
Size: 34 563
Bme1580I G_kGCm'C
Cuts at: 0 252 382 597
Size: 252 130 215
Fragments arranged by size:
252 215 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 262 567 597
Size: 262 305 30
Fragments arranged by size:
305 262 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 597
Size: 385 212
Bpu10I CC'TnA_GC
Cuts at: 0 178 495 597
Size: 178 317 102
Fragments arranged by size:
317 178 102
BsaAI yAC'GTr
Cuts at: 0 321 597
Size: 321 276
BsaHI Gr'CG_yC
Cuts at: 0 472 484 597
Size: 472 12 113
Fragments arranged by size:
472 113 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 483 597
Size: 483 114
BseYI C'CCAG_C
Cuts at: 0 192 375 449 597
Size: 192 183 74 148
Fragments arranged by size:
192 183 148 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 263 343 526 597
Size: 155 108 80 183 71
Fragments arranged by size:
183 155 108 80 71
BsiHKAI G_wGCw'C
Cuts at: 0 131 261 597
Size: 131 130 336
Fragments arranged by size:
336 131 130
Bsp1286I G_dGCh'C
Cuts at: 0 131 252 261 371 382 597
Size: 131 121 9 110 11 215
Fragments arranged by size:
215 131 121 110 11 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 320 331 597
Size: 320 11 266
Fragments arranged by size:
320 266 11
BsrBI CCG'CTC
Cuts at: 0 140 295 597
Size: 140 155 302
Fragments arranged by size:
302 155 140
BsrFI r'CCGG_y
Cuts at: 0 401 413 468 597
Size: 401 12 55 129
Fragments arranged by size:
401 129 55 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 330 597
Size: 330 267
BtsI GCAGTG_nn'
Cuts at: 0 331 597
Size: 331 266
EaeI y'GGCC_r
Cuts at: 0 43 233 597
Size: 43 190 364
Fragments arranged by size:
364 190 43
EarI CTCTTCn'nnn_
Cuts at: 0 354 597
Size: 354 243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 597
Size: 166 431
EcoICRI GAG'CTC
Cuts at: 0 259 597
Size: 259 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 385 597
Size: 376 9 212
Fragments arranged by size:
376 212 9
HaeII r_GCGC'y
Cuts at: 0 475 487 597
Size: 475 12 110
Fragments arranged by size:
475 110 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 126 158 289 321 597
Size: 126 32 131 32 276
Fragments arranged by size:
276 131 126 32 32
KasI G'GCGC_C
Cuts at: 0 471 483 597
Size: 471 12 114
Fragments arranged by size:
471 114 12
MscI TGG'CCA
Cuts at: 0 45 235 597
Size: 45 190 362
Fragments arranged by size:
362 190 45
MspA1I CmG'CkG
Cuts at: 0 55 165 177 196 480 597
Size: 55 110 12 19 284 117
Fragments arranged by size:
284 117 110 55 19 12
NaeI GCC'GGC
Cuts at: 0 415 470 597
Size: 415 55 127
Fragments arranged by size:
415 127 55
NarI GG'CG_CC
Cuts at: 0 472 484 597
Size: 472 12 113
Fragments arranged by size:
472 113 12
NgoMIV G'CCGG_C
Cuts at: 0 413 468 597
Size: 413 55 129
Fragments arranged by size:
413 129 55
PasI CC'CwG_GG
Cuts at: 0 64 597
Size: 64 533
PflMI CCAn_nnn'nTGG
Cuts at: 0 76 213 597
Size: 76 137 384
Fragments arranged by size:
384 137 76
PmlI CAC'GTG
Cuts at: 0 321 597
Size: 321 276
PspXI vC'TCGA_Gb
Cuts at: 0 86 597
Size: 86 511
PstI C_TGCA'G
Cuts at: 0 389 422 597
Size: 389 33 175
Fragments arranged by size:
389 175 33
PvuII CAG'CTG
Cuts at: 0 55 165 177 196 597
Size: 55 110 12 19 401
Fragments arranged by size:
401 110 55 19 12
SacI G_AGCT'C
Cuts at: 0 261 597
Size: 261 336
SapI GCTCTTCn'nnn_
Cuts at: 0 354 597
Size: 354 243
SbfI CC_TGCA'GG
Cuts at: 0 389 597
Size: 389 208
SfcI C'TryA_G
Cuts at: 0 262 385 418 597
Size: 262 123 33 179
Fragments arranged by size:
262 179 123 33
SfoI GGC'GCC
Cuts at: 0 473 485 597
Size: 473 12 112
Fragments arranged by size:
473 112 12
SgrAI Cr'CCGG_yG
Cuts at: 0 468 597
Size: 468 129
SmlI C'TyrA_G
Cuts at: 0 86 597
Size: 86 511
XhoI C'TCGA_G
Cuts at: 0 86 597
Size: 86 511
Enzymes that do cut:
AarI AcuI AhdI AlfI AlwNI ApoI AvaI BaeI
BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BmrI
BpmI Bpu10I BsaAI BsaHI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BstAPI BtsI EaeI EarI
EciI EcoICRI Eco57MI HaeII Hin4I KasI MscI MspA1I
NaeI NarI NgoMIV PasI PflMI PmlI PspXI PstI
PvuII SacI SapI SbfI SfcI SfoI SgrAI SmlI
XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BmtI BplI BpuEI BsaI BsaBI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI
SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI