(Circular) (Six-base) MAPSORT of: pFN21AE6266.seq Check: 9145 from: 1 to: 5467 pFN21AE6266 5467 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3277 278 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2712 2810 1937 Size: 775 98 4594 Fragments arranged by size: 4594 775 98 Acc65I G'GTAC_C Cuts at: 2043 2788 2043 Size: 745 4722 AclI AA'CG_TT Cuts at: 1563 3594 3967 1563 Size: 2031 373 3063 Fragments arranged by size: 3063 2031 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3530 4914 5182 1632 Size: 1898 1384 268 1917 Fragments arranged by size: 1917 1898 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5270 AflIII A'CryG_T Cuts at: 1203 2511 4366 5343 1203 Size: 1308 1855 977 1327 Fragments arranged by size: 1855 1327 1308 977 AgeI A'CCGG_T Cuts at: 4298 4298 Size: 5467 AhdI GACnn_n'nnGTC Cuts at: 4196 4196 Size: 5467 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5467 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5467 AlwNI CAG_nnn'CTG Cuts at: 3210 4782 3210 Size: 1572 3895 ApaI G_GGCC'C Cuts at: 2634 2634 Size: 5467 ApaLI G'TGCA_C Cuts at: 3524 4680 3524 Size: 1156 4311 ApoI r'AATT_y Cuts at: 1493 2729 2776 3002 3154 5263 1493 Size: 1236 47 226 152 2109 1697 Fragments arranged by size: 2109 1697 1236 226 152 47 AseI AT'TA_AT Cuts at: 160 1032 4021 160 Size: 872 2989 1606 Fragments arranged by size: 2989 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 AvaI C'yCGr_G Cuts at: 1870 1943 2173 2792 1870 Size: 73 230 619 4545 Fragments arranged by size: 4545 619 230 73 BamHI G'GATC_C Cuts at: 2797 3164 2797 Size: 367 5100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2043 2316 2788 4243 618 Size: 325 217 627 256 273 472 1455 1842 Fragments arranged by size: 1842 1455 627 472 325 273 256 217 BanII G_rGCy'C Cuts at: 1391 2634 2786 1391 Size: 1243 152 4072 Fragments arranged by size: 4072 1243 152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3658 3692 1903 Size: 34 1721 34 3678 Fragments arranged by size: 3678 1721 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3359 4569 3359 Size: 1210 4257 BclI T'GATC_A Cuts at: 1548 1548 Size: 5467 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2697 4078 136 Size: 107 122 71 631 605 1025 1381 1525 Fragments arranged by size: 1525 1381 1025 631 605 122 107 71 BglII A'GATC_T Cuts at: 5462 5462 Size: 5467 BlpI GC'TnA_GC Cuts at: 2884 2884 Size: 5467 Bme1580I G_kGCm'C Cuts at: 1790 2429 2634 3528 4684 1790 Size: 639 205 894 1156 2573 Fragments arranged by size: 2573 1156 894 639 205 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5467 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3198 4156 442 Size: 416 53 83 399 24 1781 958 1753 Fragments arranged by size: 1781 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4127 1239 Size: 2888 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2282 3592 4457 4755 2282 Size: 1310 865 298 2994 Fragments arranged by size: 2994 1310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4130 882 Size: 617 2631 2219 Fragments arranged by size: 2631 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3258 493 Size: 711 2054 2702 Fragments arranged by size: 2702 2054 711 BsaBI GATnn'nnATC Cuts at: 5461 5461 Size: 5467 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2178 3274 3656 4291 Size: 53 83 186 936 645 1096 382 635 Cuts at: 4291 275 Size: 1451 Fragments arranged by size: 1451 1096 936 645 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2400 3900 4298 4572 4719 5130 2400 Size: 1500 398 274 147 411 2737 Fragments arranged by size: 2737 1500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5242 Fragments arranged by size: 5242 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 1186 Size: 1357 4110 BseYI C'CCAG_C Cuts at: 2245 2460 2567 4670 5166 2245 Size: 215 107 2103 496 2546 Fragments arranged by size: 2546 2103 496 215 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4894 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2917 3678 4706 1540 Size: 275 186 916 761 1028 2301 Fragments arranged by size: 2301 1028 916 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3528 3613 4684 3528 Size: 85 1071 4311 Fragments arranged by size: 4311 1071 85 BsiWI C'GTAC_G Cuts at: 2311 2311 Size: 5467 BsmI GAATG_Cn' Cuts at: 2490 2978 3071 2490 Size: 488 93 4886 Fragments arranged by size: 4886 488 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2080 2080 Size: 5467 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2429 2634 2786 3528 3613 4684 1391 Size: 399 639 205 152 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 639 399 205 152 85 BspEI T'CCGG_A Cuts at: 2400 5130 2400 Size: 2730 2737 BspHI T'CATG_A Cuts at: 3356 5086 3356 Size: 1730 3737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2822 844 Size: 1978 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3354 1394 Size: 429 1531 3507 Fragments arranged by size: 3507 1531 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2412 3962 4136 65 Size: 1183 1164 1550 174 1396 Fragments arranged by size: 1550 1396 1183 1164 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2582 4111 4298 1700 Size: 110 772 1529 187 2869 Fragments arranged by size: 2869 1529 772 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5467 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5467 BssSI C'ACGA_G Cuts at: 3527 4539 3527 Size: 1012 4455 BstAPI GCAn_nnn'nTGC Cuts at: 794 2200 2577 794 Size: 1406 377 3684 Fragments arranged by size: 3684 1406 377 BstBI TT'CG_AA Cuts at: 4271 4271 Size: 5467 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5467 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2245 Size: 5467 BstYI r'GATC_y Cuts at: 1972 2066 2797 3164 3549 3566 5018 5104 5116 Size: 94 731 367 385 17 1452 86 12 Cuts at: 5116 5462 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 731 385 367 346 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2433 513 Size: 552 939 429 3547 Fragments arranged by size: 3547 939 552 429 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4728 Fragments arranged by size: 4728 683 56 BtsI GCAGTG_nn' Cuts at: 2201 2429 2507 2983 3777 3797 2201 Size: 228 78 476 794 20 3871 Fragments arranged by size: 3871 794 476 228 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5467 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5432 DraI TTT'AAA Cuts at: 2771 2771 Size: 5467 DraIII CAC_nnn'GTG Cuts at: 2739 2739 Size: 5467 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4474 809 Size: 507 3158 1802 Fragments arranged by size: 3158 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2689 2914 Size: 54 1425 50 275 18 177 682 225 Cuts at: 2914 3803 5432 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 682 275 225 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2914 1537 Size: 275 1102 4090 Fragments arranged by size: 4090 1102 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3397 1353 Size: 2044 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2601 4054 4428 4574 387 Size: 1064 138 1012 1453 374 146 1280 Fragments arranged by size: 1453 1280 1064 1012 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3530 4127 4914 5182 1239 Size: 393 1898 597 787 268 1524 Fragments arranged by size: 1898 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2630 2631 2659 2630 Size: 1 28 5438 Fragments arranged by size: 5438 28 1 EcoRI G'AATT_C Cuts at: 2776 3154 2776 Size: 378 5089 FspI TGC'GCA Cuts at: 3973 3973 Size: 5467 HaeII r_GCGC'y Cuts at: 1678 4614 1678 Size: 2936 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2325 2357 4108 Size: 32 135 32 486 32 235 32 1751 Cuts at: 4108 4140 4182 4214 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1751 486 235 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2811 3057 669 Size: 988 281 873 246 3079 Fragments arranged by size: 3079 988 873 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5467 HpaI GTT'AAC Cuts at: 3057 3057 Size: 5467 KpnI G_GTAC'C Cuts at: 2047 2792 2047 Size: 745 4722 MfeI C'AATT_G Cuts at: 3066 3066 Size: 5467 MluI A'CGCG_T Cuts at: 5343 5343 Size: 5467 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2349 4581 4765 1305 Size: 1044 2232 184 2007 Fragments arranged by size: 2232 2007 1044 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2691 10 Size: 54 1425 343 177 682 2786 Fragments arranged by size: 2786 1425 682 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3425 3784 3943 518 Size: 648 2259 359 159 2042 Fragments arranged by size: 2259 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2435 2557 2883 3560 4708 4953 1606 Size: 829 122 326 677 1148 245 2120 Fragments arranged by size: 2120 1148 829 677 326 245 122 NaeI GCC'GGC Cuts at: 2584 2584 Size: 5467 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3976 Fragments arranged by size: 3976 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5467 NgoMIV G'CCGG_C Cuts at: 2582 2582 Size: 5467 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5467 NotI GC'GGCC_GC Cuts at: 2914 2914 Size: 5467 NspI r_CATG'y Cuts at: 1132 1229 2575 2825 4370 1132 Size: 97 1346 250 1545 2229 Fragments arranged by size: 2229 1545 1346 250 97 PasI CC'CwG_GG Cuts at: 1348 2025 1348 Size: 677 4790 PciI A'CATG_T Cuts at: 4366 4366 Size: 5467 PflMI CCAn_nnn'nTGG Cuts at: 1108 2293 1108 Size: 1185 4282 PmeI GTTT'AAAC Cuts at: 2771 2771 Size: 5467 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3536 3568 5049 5081 3536 Size: 32 1481 32 3922 Fragments arranged by size: 3922 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5467 PsiI TTA'TAA Cuts at: 3037 3037 Size: 5467 PspOMI G'GGCC_C Cuts at: 2630 2630 Size: 5467 PstI C_TGCA'G Cuts at: 830 2090 2117 2502 2540 2819 830 Size: 1260 27 385 38 279 3478 Fragments arranged by size: 3478 1260 385 279 38 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5467 SacII CC_GC'GG Cuts at: 2436 2436 Size: 5467 SalI G'TCGA_C Cuts at: 1936 2809 1936 Size: 873 4594 SbfI CC_TGCA'GG Cuts at: 2819 2819 Size: 5467 ScaI AGT'ACT Cuts at: 3715 5427 3715 Size: 1712 3755 SfcI C'TryA_G Cuts at: 826 1045 2086 2113 2498 2536 2815 3950 4631 Size: 219 1041 27 385 38 279 1135 681 Cuts at: 4631 4822 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1041 681 385 279 219 191 38 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 SgrAI Cr'CCGG_yG Cuts at: 1700 2582 1700 Size: 882 4585 SmaI CCC'GGG Cuts at: 1872 2794 1872 Size: 922 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 3571 4472 4734 820 Size: 197 926 318 1310 901 262 1553 Fragments arranged by size: 1553 1310 926 901 318 262 197 SnaBI TAC'GTA Cuts at: 493 3258 493 Size: 2765 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5467 SphI G_CATG'C Cuts at: 1132 2825 1132 Size: 1693 3774 SspI AAT'ATT Cuts at: 5 52 3391 5 Size: 47 3339 2081 Fragments arranged by size: 3339 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5467 StyI C'CwwG_G Cuts at: 513 1065 2004 2053 2906 513 Size: 552 939 49 853 3074 Fragments arranged by size: 3074 939 853 552 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 2334 3494 3511 3664 3849 4294 Size: 1255 608 146 1160 17 153 185 445 Cuts at: 4294 325 Size: 1498 Fragments arranged by size: 1498 1255 1160 608 445 185 153 146 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 3713 5425 Size: 274 80 33 51 1443 348 1388 1712 Cuts at: 5425 96 Size: 138 Fragments arranged by size: 1712 1443 1388 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2205 2237 5427 5459 2205 Size: 32 3190 32 2213 Fragments arranged by size: 3190 2213 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5467 XbaI T'CTAG_A Cuts at: 2803 2803 Size: 5467 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5467 XmaI C'CCGG_G Cuts at: 1870 2792 1870 Size: 922 4545 XmnI GAAnn'nnTTC Cuts at: 3596 3596 Size: 5467 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3275 276 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI