################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BciVI BclI BfrI BlnI BlpI BpmI Bpu1102I BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI HgaI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 226 GACGTC GACGT^C AccII 18 CGCG CG^CG Alw44I 787 GTGCAC G^TGCAC ApaI 275 GGGCCC GGGCC^C ApaLI 787 GTGCAC G^TGCAC AviII 158 TGCGCA TGC^GCA BbeI 501 GGCGCC GGCGC^C BbrPI 355 CACGTG CAC^GTG BbvCI 76 GCTGAGG CCTCAGC(-5/-2) BcgI 281 GCAAGGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 652 CTAG C^TAG BglI 793 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 935 AGATCT A^GATCT BsaWI 1049 ACCGGA [AT]^CCGG[AT] BspLU11I 614 ACATGT A^CATGT BssSI 133 CACGAG CACGAG(-5/-1) BstAPI 245 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 18 CGCG CG^CG DdeI 77 CTGAG C^T[ACGT]AG DraIII 355 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT14I 266 CCAAGG C^C[AT][AT]GG FauI 278 CCCGC CCCGC(4/6) FspI 158 TGCGCA TGC^GCA HaeII 501 GGCGCC [GA]GCGC^[CT] KasI 501 GGCGCC G^GCGCC MaeI 652 CTAG C^TAG MseI 641 TTAA T^TAA MvnI 18 CGCG CG^CG NarI 501 GGCGCC GG^CGCC PmaCI 355 CACGTG CAC^GTG PmlI 355 CACGTG CAC^GTG PpuMI 980 GGGACCT [GA]G^G[AT]CC[CT] SacI 394 GAGCTC GAGCT^C SfoI 501 GGCGCC GGC^GCC SmaI 485 CCCGGG CCC^GGG SphI 953 GCATGC GCATG^C StyI 266 CCAAGG C^C[AT][AT]GG Tru9I 641 TTAA T^TAA XmaCI 485 CCCGGG C^CCGGG XmaI 485 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 226 GACGTC G[GA]^CG[CT]C 501 GGCGCC AfaI 567 GTAC GT^AC 870 GTAC AflIII 599 ACGTGT A^C[GA][CT]GT 614 ACATGT AlwI 582 GATCC GGATC(4/5) 293 GGATC BalI 45 TGGCCA TGG^CCA 635 TGGCCA BbsI 733 GAAGAC GAAGAC(2/6) 921 GAAGAC BpuAI 733 GAAGAC GAAGAC(2/6) 921 GAAGAC BsaAI 355 CACGTG [CT]AC^GT[GA] 784 TACGTG BsaHI 226 GACGTC G[GA]^CG[CT]C 501 GGCGCC BstEII 360 GGTGACC G^GT[ACGT]ACC 681 GGTCACC BstPI 360 GGTGACC G^GT[ACGT]ACC 681 GGTCACC BstXI 671 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 995 CCATCGACCTGG BstYI 581 AGATCC [GA]^GATC[CT] 935 AGATCT DraII 274 GGGGCCC [GA]G^G[ACGT]CC[CT] 980 GGGACCT EarI 202 CTCTTC CTCTTC(1/4) 448 CTCTTC EcoNI 201 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 696 CCTCATAAAGG EcoO109I 274 GGGGCCC [GA]G^G[ACGT]CC[CT] 980 GGGACCT EcoO65I 360 GGTGACC G^GT[ACGT]ACC 681 GGTCACC Hin1I 226 GACGTC G[GA]^CG[CT]C 501 GGCGCC Ksp632I 202 CTCTTC CTCTTC(1/4) 448 CTCTTC MflI 581 AGATCC [GA]^GATC[CT] 935 AGATCT MluNI 45 TGGCCA TGG^CCA 635 TGGCCA MscI 45 TGGCCA TGG^CCA 635 TGGCCA MslI 30 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 948 CATCGGCATG MspA1I 676 CAGCTG C[AC]G^C[GT]G 1018 CAGCTG PvuII 676 CAGCTG CAG^CTG 1018 CAGCTG RsaI 567 GTAC GT^AC 870 GTAC XhoII 581 AGATCC [GA]^GATC[CT] 935 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AluI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I TspRI TthHB8I