(Circular) (Six-base) MAPSORT of: pFN21AE1854.seq Check: 2402 from: 1 to: 5764 pFN21AE1854 5764 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2235 3574 278 Size: 53 83 186 936 699 1339 2468 Fragments arranged by size: 2468 1339 936 699 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 3827 5211 5479 1632 Size: 539 1656 1384 268 1917 Fragments arranged by size: 1917 1656 1384 539 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 2604 2619 4663 5640 1203 Size: 1401 15 2044 977 1327 Fragments arranged by size: 2044 1401 1327 977 15 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5764 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2290 2322 2290 Size: 32 5732 AlwNI CAG_nnn'CTG Cuts at: 3507 5079 3507 Size: 1572 4192 ApaI G_GGCC'C Cuts at: 2284 2284 Size: 5764 ApaLI G'TGCA_C Cuts at: 2792 3821 4977 2792 Size: 1029 1156 3579 Fragments arranged by size: 3579 1156 1029 ApoI r'AATT_y Cuts at: 1493 3073 3299 3451 5560 1493 Size: 1580 226 152 2109 1697 Fragments arranged by size: 2109 1697 1580 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 2239 2490 2864 3089 1870 Size: 73 296 251 374 225 4545 Fragments arranged by size: 4545 374 296 251 225 73 BamHI G'GATC_C Cuts at: 3094 3461 3094 Size: 367 5397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2202 2243 2506 2948 3085 Size: 325 217 627 415 41 263 442 137 Cuts at: 3085 4540 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 442 415 325 263 217 137 41 BanII G_rGCy'C Cuts at: 1391 2284 2403 2655 3083 1391 Size: 893 119 252 428 4072 Fragments arranged by size: 4072 893 428 252 119 BbeI G_GCGC'C Cuts at: 2510 2510 Size: 5764 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2745 2933 928 Size: 971 846 188 3759 Fragments arranged by size: 3759 971 846 188 BbvCI CC'TCA_GC Cuts at: 2082 2082 Size: 5764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2275 2309 3955 3989 1903 Size: 34 338 34 1646 34 3678 Fragments arranged by size: 3678 1646 338 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2804 4375 Size: 107 122 71 631 605 337 795 1571 Cuts at: 4375 136 Size: 1525 Fragments arranged by size: 1571 1525 795 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2940 5759 2940 Size: 2819 2945 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 2246 2284 2796 3825 4981 1790 Size: 456 38 512 1029 1156 2573 Fragments arranged by size: 2573 1156 1029 512 456 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5764 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2399 3495 4453 Size: 416 53 83 399 24 982 1096 958 Cuts at: 4453 442 Size: 1753 Fragments arranged by size: 1753 1096 982 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4424 1239 Size: 3185 2579 Bpu10I CC'TnA_GC Cuts at: 2082 2082 Size: 5764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3889 4754 5052 3889 Size: 865 298 4601 Fragments arranged by size: 4601 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2362 2791 3555 493 Size: 711 1158 429 764 2702 Fragments arranged by size: 2702 1158 764 711 429 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 2507 3571 3953 Size: 53 83 186 936 699 275 1064 382 Cuts at: 3953 4588 275 Size: 635 1451 Fragments arranged by size: 1451 1064 936 699 635 382 275 186 83 53 BsaWI w'CCGG_w Cuts at: 3054 4197 4595 4869 5016 5427 3054 Size: 1143 398 274 147 411 3391 Fragments arranged by size: 3391 1143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2099 2129 2168 2198 2591 Size: 30 165 30 696 30 39 30 393 Cuts at: 2591 2621 1178 Size: 30 4321 Fragments arranged by size: 4321 696 393 165 39 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2405 2951 1186 Size: 970 249 546 3999 Fragments arranged by size: 3999 970 546 249 BseYI C'CCAG_C Cuts at: 4967 5463 4967 Size: 496 5268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3214 3975 5003 1540 Size: 275 186 1213 761 1028 2301 Fragments arranged by size: 2301 1213 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2403 2484 2796 3825 3910 4981 2403 Size: 81 312 1029 85 1071 3186 Fragments arranged by size: 3186 1071 1029 312 85 81 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2246 2284 2403 2484 2655 2796 3083 Size: 399 456 38 119 81 171 141 287 Cuts at: 3083 3825 3910 4981 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 456 399 287 171 141 119 85 81 38 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3119 844 Size: 2275 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4259 4433 65 Size: 1183 3011 174 1396 Fragments arranged by size: 3011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2199 2246 2565 2846 4408 4595 1700 Size: 110 389 47 319 281 1562 187 2869 Fragments arranged by size: 2869 1562 389 319 281 187 110 47 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 2138 3824 4836 2138 Size: 1686 1012 3066 Fragments arranged by size: 3066 1686 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2256 794 Size: 1462 4302 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 2365 2686 1184 Size: 1181 321 4262 Fragments arranged by size: 4262 1181 321 BstXI CCAn_nnnn'nTGG Cuts at: 2683 3007 2683 Size: 324 5440 BstYI r'GATC_y Cuts at: 1972 2586 2940 3094 3461 3846 3863 5315 5401 Size: 614 354 154 367 385 17 1452 86 Cuts at: 5401 5413 5759 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 614 385 367 354 346 154 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 2071 3280 4074 4094 2071 Size: 1209 794 20 3741 Fragments arranged by size: 3741 1209 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DraIII CAC_nnn'GTG Cuts at: 2365 2365 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2553 2640 Size: 54 1425 50 275 18 220 503 87 Cuts at: 2640 3211 4100 5729 8 Size: 571 889 1629 43 Fragments arranged by size: 1629 1425 889 571 503 275 220 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2213 2459 3694 1353 Size: 860 246 1235 3423 Fragments arranged by size: 3423 1235 860 246 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2072 4351 4725 4871 387 Size: 1064 138 483 2279 374 146 1280 Fragments arranged by size: 2279 1280 1064 483 374 146 138 EcoICRI GAG'CTC Cuts at: 2401 2401 Size: 5764 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 3827 4424 5211 5479 1239 Size: 393 539 1656 597 787 268 1524 Fragments arranged by size: 1656 1524 787 597 539 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2210 2705 2210 Size: 495 5269 EcoO109I rG'GnC_Cy Cuts at: 2280 2986 2280 Size: 706 5058 EcoRI G'AATT_C Cuts at: 3073 3451 3073 Size: 378 5386 FspI TGC'GCA Cuts at: 2165 4270 2165 Size: 2105 3659 FspAI rTGC'GCAy Cuts at: 2165 2165 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 2510 4911 1678 Size: 832 2401 2531 Fragments arranged by size: 2531 2401 832 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2222 2254 4405 Size: 32 135 32 506 32 112 32 2151 Cuts at: 4405 4437 4479 4511 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2151 506 135 112 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5764 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KasI G'GCGC_C Cuts at: 2506 2506 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4878 5062 1305 Size: 3573 184 2007 Fragments arranged by size: 3573 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2052 2642 10 Size: 54 1425 343 220 590 3132 Fragments arranged by size: 3132 1425 590 343 220 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2957 3722 4081 4240 518 Size: 648 873 918 765 359 159 2042 Fragments arranged by size: 2042 918 873 765 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2683 3025 3180 3857 5005 5250 1606 Size: 1077 342 155 677 1148 245 2120 Fragments arranged by size: 2120 1148 1077 677 342 245 155 NarI GG'CG_CC Cuts at: 2507 2507 Size: 5764 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 2263 2623 2962 3122 4667 1132 Size: 97 1034 360 339 160 1545 2229 Fragments arranged by size: 2229 1545 1034 360 339 160 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 2619 4663 2619 Size: 2044 3720 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5764 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PmlI CAC'GTG Cuts at: 2362 2362 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2986 2986 Size: 5764 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PspOMI G'GGCC_C Cuts at: 2280 2280 Size: 5764 PstI C_TGCA'G Cuts at: 830 3116 830 Size: 2286 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 2683 3025 2683 Size: 342 5422 SacI G_AGCT'C Cuts at: 2403 2403 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SfcI C'TryA_G Cuts at: 826 1045 3112 4247 4928 5119 826 Size: 219 2067 1135 681 191 1471 Fragments arranged by size: 2067 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2508 2508 Size: 5764 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 2492 3091 1872 Size: 620 599 4545 Fragments arranged by size: 4545 620 599 SmlI C'TyrA_G Cuts at: 820 1017 1943 3868 4769 5031 820 Size: 197 926 1925 901 262 1553 Fragments arranged by size: 1925 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 2962 3122 1132 Size: 1830 160 3774 Fragments arranged by size: 3774 1830 160 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5764 StyI C'CwwG_G Cuts at: 513 1065 2004 2271 3203 513 Size: 552 939 267 932 3074 Fragments arranged by size: 3074 939 932 552 267 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3791 3808 3961 4146 4591 325 Size: 1255 2211 17 153 185 445 1498 Fragments arranged by size: 2211 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2571 4010 5722 Size: 274 80 33 51 1443 594 1439 1712 Cuts at: 5722 96 Size: 138 Fragments arranged by size: 1712 1443 1439 594 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5724 5756 5724 Size: 32 5732 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 2490 3089 1870 Size: 620 599 4545 Fragments arranged by size: 4545 620 599 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2233 3572 276 Size: 53 83 186 936 699 1339 2468 Fragments arranged by size: 2468 1339 936 699 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI