(Circular) (Six-base) MAPSORT of: pFN21AE2344.seq Check: 4265 from: 1 to: 8713 pFN21AE2344 8713 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3374 5880 3374 Size: 2506 6207 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2561 6523 278 Size: 53 83 186 936 1025 3962 2468 Fragments arranged by size: 3962 2468 1025 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5469 6056 1937 Size: 3532 587 4594 Fragments arranged by size: 4594 3532 587 Acc65I G'GTAC_C Cuts at: 6034 6034 Size: 8713 AclI AA'CG_TT Cuts at: 1563 6840 7213 1563 Size: 5277 373 3063 Fragments arranged by size: 5277 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 3490 3500 4148 6776 8160 8428 1632 Size: 872 986 10 648 2628 1384 268 1917 Fragments arranged by size: 2628 1917 1384 986 872 648 268 10 AfeI AGC'GCT Cuts at: 2664 3449 3641 4236 2664 Size: 785 192 595 7141 Fragments arranged by size: 7141 785 595 192 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8516 AflIII A'CryG_T Cuts at: 1203 7612 8589 1203 Size: 6409 977 1327 Fragments arranged by size: 6409 1327 977 AgeI A'CCGG_T Cuts at: 7544 7544 Size: 8713 AhdI GACnn_n'nnGTC Cuts at: 2323 2401 2557 7442 2323 Size: 78 156 4885 3594 Fragments arranged by size: 4885 3594 156 78 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2318 4397 1184 Size: 1134 2079 5500 Fragments arranged by size: 5500 2079 1134 AlwNI CAG_nnn'CTG Cuts at: 3004 4435 4692 4747 6456 8028 3004 Size: 1431 257 55 1709 1572 3689 Fragments arranged by size: 3689 1709 1572 1431 257 55 ApaI G_GGCC'C Cuts at: 2042 2127 2184 2216 2835 2866 3919 4641 4791 Size: 85 57 32 619 31 1053 722 150 Cuts at: 4791 5658 5677 5874 2042 Size: 867 19 197 4881 Fragments arranged by size: 4881 1053 867 722 619 197 150 85 57 32 31 19 ApaLI G'TGCA_C Cuts at: 2339 2435 6770 7926 2339 Size: 96 4335 1156 3126 Fragments arranged by size: 4335 3126 1156 96 ApoI r'AATT_y Cuts at: 1493 6022 6248 6400 8509 1493 Size: 4529 226 152 2109 1697 Fragments arranged by size: 4529 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 5488 5767 5488 Size: 279 8434 AseI AT'TA_AT Cuts at: 160 1032 7267 160 Size: 872 6235 1606 Fragments arranged by size: 6235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8713 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2176 2242 2751 3737 3954 4784 Size: 73 91 142 66 509 986 217 830 Cuts at: 4784 5201 5327 5438 5477 5819 5999 6038 1870 Size: 417 126 111 39 342 180 39 4545 Fragments arranged by size: 4545 986 830 509 417 342 217 180 142 126 111 91 73 66 39 39 AvrII C'CTAG_G Cuts at: 4921 4921 Size: 8713 BamHI G'GATC_C Cuts at: 6043 6410 6043 Size: 367 8346 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2098 2444 2894 2993 Size: 325 217 627 225 86 346 450 99 Cuts at: 2993 3362 3937 4617 4833 4951 4971 5069 5173 Size: 369 575 680 216 118 20 98 104 Cuts at: 5173 5626 5685 5760 5966 6034 7489 618 Size: 453 59 75 206 68 1455 1842 Fragments arranged by size: 1842 1455 680 627 575 453 450 369 346 325 225 217 216 206 118 104 99 98 86 75 68 59 20 BanII G_rGCy'C Cuts at: 1391 2042 2127 2184 2216 2480 2501 2769 2835 Size: 651 85 57 32 264 21 268 66 Cuts at: 2835 2858 2866 2877 3036 3744 3919 4083 4394 Size: 23 8 11 159 708 175 164 311 Cuts at: 4394 4641 4791 5061 5168 5272 5397 5658 5677 Size: 247 150 270 107 104 125 261 19 Cuts at: 5677 5874 5981 6032 1391 Size: 197 107 51 4072 Fragments arranged by size: 4072 708 651 311 270 268 264 261 247 197 175 164 159 150 125 107 107 104 85 66 57 51 32 23 21 19 11 8 BbeI G_GCGC'C Cuts at: 2016 2102 2448 2898 4621 4837 4975 5073 5177 Size: 86 346 450 1723 216 138 98 104 Cuts at: 5177 5630 2016 Size: 453 5099 Fragments arranged by size: 5099 1723 453 450 346 216 138 104 98 86 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2344 2364 4345 4540 4582 928 Size: 971 445 20 1981 195 42 5059 Fragments arranged by size: 5059 1981 971 445 195 42 20 BbvCI CC'TCA_GC Cuts at: 2784 5828 2784 Size: 3044 5669 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2910 2944 4336 4370 6904 Size: 34 50 34 889 34 1392 34 2534 Cuts at: 6904 6938 1903 Size: 34 3678 Fragments arranged by size: 3678 2534 1392 889 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4060 6605 7815 4060 Size: 2545 1210 4958 Fragments arranged by size: 4958 2545 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2028 2037 2201 Size: 107 122 71 631 605 356 9 164 Cuts at: 2201 2225 2911 2984 4034 5114 5370 5613 7324 Size: 24 686 73 1050 1080 256 243 1711 Cuts at: 7324 136 Size: 1525 Fragments arranged by size: 1711 1525 1080 1050 686 631 605 356 256 243 164 122 107 73 71 24 9 BglII A'GATC_T Cuts at: 8708 8708 Size: 8713 BlpI GC'TnA_GC Cuts at: 4995 6130 4995 Size: 1135 7578 Bme1580I G_kGCm'C Cuts at: 1790 2042 2127 2184 2216 2343 2439 2587 2707 Size: 252 85 57 32 127 96 148 120 Cuts at: 2707 2835 2866 2998 3919 3942 4146 4641 4791 Size: 128 31 132 921 23 204 495 150 Cuts at: 4791 4806 5249 5315 5658 5677 5690 5840 5874 Size: 15 443 66 343 19 13 150 34 Cuts at: 5874 5969 6774 7930 1790 Size: 95 805 1156 2573 Fragments arranged by size: 2573 1156 921 805 495 443 343 252 204 150 150 148 132 128 127 120 96 95 85 66 57 34 32 31 23 19 15 13 BmgBI CAC'GTC Cuts at: 1318 2254 3195 1318 Size: 936 941 6836 Fragments arranged by size: 6836 941 936 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5920 6444 7402 Size: 416 53 83 399 24 4503 524 958 Cuts at: 7402 442 Size: 1753 Fragments arranged by size: 4503 1753 958 524 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8713 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2494 3658 4834 2494 Size: 1164 1176 6373 Fragments arranged by size: 6373 1176 1164 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2561 2865 3335 4481 4657 5411 5582 7373 Size: 1322 304 470 1146 176 754 171 1791 Cuts at: 7373 1239 Size: 2579 Fragments arranged by size: 2579 1791 1322 1146 754 470 304 176 171 Bpu10I CC'TnA_GC Cuts at: 2784 3030 5828 2784 Size: 246 2798 5669 Fragments arranged by size: 5669 2798 246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3221 4232 6838 7703 8001 3221 Size: 1011 2606 865 298 3933 Fragments arranged by size: 3933 2606 1011 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3671 7376 882 Size: 617 2172 3705 2219 Fragments arranged by size: 3705 2219 2172 617 BsaAI yAC'GTr Cuts at: 493 1204 3108 4063 6504 493 Size: 711 1904 955 2441 2702 Fragments arranged by size: 2702 2441 1904 955 711 BsaBI GATnn'nnATC Cuts at: 8707 8707 Size: 8713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 2099 2445 2558 Size: 53 83 186 936 480 86 346 113 Cuts at: 2558 2895 3561 4266 4545 4618 4834 4972 5070 Size: 337 666 705 279 73 216 138 98 Cuts at: 5070 5174 5627 5668 6520 6902 7537 275 Size: 104 453 41 852 382 635 1451 Fragments arranged by size: 1451 936 852 705 666 635 480 453 382 346 337 279 216 186 138 113 104 98 86 83 73 53 41 BsaWI w'CCGG_w Cuts at: 3424 5940 7146 7544 7818 7965 8376 3424 Size: 2516 1206 398 274 147 411 3761 Fragments arranged by size: 3761 2516 1206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4376 4406 4454 4484 5384 Size: 30 165 30 2973 30 48 30 900 Cuts at: 5384 5414 1178 Size: 30 4477 Fragments arranged by size: 4477 2973 900 165 48 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2558 2771 2801 3650 4070 5280 1186 Size: 922 450 213 30 849 420 1210 4619 Fragments arranged by size: 4619 1210 922 849 450 420 213 30 BseYI C'CCAG_C Cuts at: 2494 2646 2706 2835 3213 4751 5218 5380 5499 Size: 152 60 129 378 1538 467 162 119 Cuts at: 5499 5774 5781 7916 8412 2494 Size: 275 7 2135 496 2795 Fragments arranged by size: 2795 2135 1538 496 467 378 275 162 152 129 119 60 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4367 1163 Size: 573 2631 5509 Fragments arranged by size: 5509 2631 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2153 2196 2220 4778 4992 5277 Size: 275 186 152 43 24 2558 214 285 Cuts at: 5277 5455 5469 5532 5696 6163 6924 7952 1540 Size: 178 14 63 164 467 761 1028 2301 Fragments arranged by size: 2558 2301 1028 761 467 285 275 214 186 178 164 152 63 43 24 14 BsiHKAI G_wGCw'C Cuts at: 2277 2343 2439 2460 2655 2700 3036 4293 4605 Size: 66 96 21 195 45 336 1257 312 Cuts at: 4605 6774 6859 7930 2277 Size: 2169 85 1071 3060 Fragments arranged by size: 3060 2169 1257 1071 336 312 195 96 85 66 45 21 BsiWI C'GTAC_G Cuts at: 2187 5035 2187 Size: 2848 5865 BsmI GAATG_Cn' Cuts at: 6224 6317 6224 Size: 93 8620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2127 2184 2216 2277 2343 2439 Size: 399 252 85 57 32 61 66 96 Cuts at: 2439 2460 2480 2501 2587 2655 2700 2707 2769 Size: 21 20 21 86 68 45 7 62 Cuts at: 2769 2835 2858 2866 2877 2998 3036 3744 3919 Size: 66 23 8 11 121 38 708 175 Cuts at: 3919 3942 4083 4146 4293 4394 4605 4641 4791 Size: 23 141 63 147 101 211 36 150 Cuts at: 4791 4806 5061 5168 5249 5272 5315 5397 5658 Size: 15 255 107 81 23 43 82 261 Cuts at: 5658 5677 5690 5840 5874 5969 5981 6032 6774 Size: 19 13 150 34 95 12 51 742 Cuts at: 6774 6859 7930 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 708 399 261 255 252 211 175 150 150 147 141 121 107 101 96 95 86 85 85 82 81 68 66 66 63 62 61 57 51 45 43 38 36 34 32 23 23 23 21 21 20 19 15 13 12 11 8 7 BspEI T'CCGG_A Cuts at: 8376 8376 Size: 8713 BspHI T'CATG_A Cuts at: 3096 4044 6602 8332 3096 Size: 948 2558 1730 3477 Fragments arranged by size: 3477 2558 1730 948 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 3374 5517 5880 6068 844 Size: 1484 1046 2143 363 188 3489 Fragments arranged by size: 3489 2143 1484 1046 363 188 BsrBI CCG'CTC Cuts at: 1394 1823 2155 4465 4994 5279 6600 1394 Size: 429 332 2310 529 285 1321 3507 Fragments arranged by size: 3507 2310 1321 529 429 332 285 BsrDI GCAATG_nn' Cuts at: 65 1248 3715 7208 7382 65 Size: 1183 2467 3493 174 1396 Fragments arranged by size: 3493 2467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 2117 3680 4777 4987 5066 5170 Size: 110 205 102 1563 1097 210 79 104 Cuts at: 5170 5763 7357 7544 1700 Size: 593 1594 187 2869 Fragments arranged by size: 2869 1594 1563 1097 593 210 205 187 110 104 102 79 BsrGI T'GTAC_A Cuts at: 96 4683 96 Size: 4587 4126 BssHII G'CGCG_C Cuts at: 1925 2368 2370 2724 3598 3701 4813 4943 4945 Size: 443 2 354 874 103 1112 130 2 Cuts at: 4945 5488 5767 5855 1925 Size: 543 279 88 4783 Fragments arranged by size: 4783 1112 874 543 443 354 279 130 103 88 2 2 BssSI C'ACGA_G Cuts at: 2168 2300 2453 2580 5833 6773 7785 2168 Size: 132 153 127 3253 940 1012 3096 Fragments arranged by size: 3253 3096 1012 940 153 132 127 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8713 BstBI TT'CG_AA Cuts at: 2355 7517 2355 Size: 5162 3551 BstEII G'GTnAC_C Cuts at: 1184 3526 1184 Size: 2342 6371 BstXI CCAn_nnnn'nTGG Cuts at: 2843 4417 2843 Size: 1574 7139 BstYI r'GATC_y Cuts at: 1972 2730 2804 4482 6043 6410 6795 6812 8264 Size: 758 74 1678 1561 367 385 17 1452 Cuts at: 8264 8350 8362 8708 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1678 1561 1452 758 385 367 346 86 74 17 12 BtgI C'CryG_G Cuts at: 513 1065 2026 2220 2262 2656 3070 3855 3942 Size: 552 961 194 42 394 414 785 87 Cuts at: 3942 4155 4569 4772 4955 5112 5690 5711 513 Size: 213 414 203 183 157 578 21 3515 Fragments arranged by size: 3515 961 785 578 552 414 414 394 213 203 194 183 157 87 42 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4360 487 Size: 683 56 3134 4840 Fragments arranged by size: 4840 3134 683 56 BtsI GCAGTG_nn' Cuts at: 2722 2928 4293 4627 5548 6229 7023 7043 2722 Size: 206 1365 334 921 681 794 20 4392 Fragments arranged by size: 4392 1365 921 794 681 334 206 20 ClaI AT'CG_AT Cuts at: 1554 3555 1554 Size: 2001 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8678 DraI TTT'AAA Cuts at: 6017 6017 Size: 8713 DraIII CAC_nnn'GTG Cuts at: 2347 4609 2347 Size: 2262 6451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3662 7720 809 Size: 507 2346 4058 1802 Fragments arranged by size: 4058 2346 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2150 2193 2217 Size: 54 1425 50 275 18 320 43 24 Cuts at: 2217 2430 2590 2976 2985 3037 3306 3609 3852 Size: 213 160 386 9 52 269 303 243 Cuts at: 3852 4035 4566 4775 4875 4989 5115 5186 5274 Size: 183 531 209 100 114 126 71 88 Cuts at: 5274 5452 5529 6160 7049 8678 8 Size: 178 77 631 889 1629 43 Fragments arranged by size: 1629 1425 889 631 531 386 320 303 275 269 243 213 209 183 178 160 126 114 100 88 77 71 54 52 50 43 43 24 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2150 2193 2217 4775 4989 5274 5452 Size: 275 338 43 24 2558 214 285 178 Cuts at: 5452 5529 6160 1537 Size: 77 631 4090 Fragments arranged by size: 4090 2558 631 338 285 275 214 178 77 43 24 EarI CTCTTCn'nnn_ Cuts at: 1353 2506 2747 2886 6643 1353 Size: 1153 241 139 3757 3423 Fragments arranged by size: 3757 3423 1153 241 139 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2723 3221 5271 5277 7300 7674 Size: 1064 138 1134 498 2050 6 2023 374 Cuts at: 7674 7820 387 Size: 146 1280 Fragments arranged by size: 2050 2023 1280 1134 1064 498 374 146 138 6 EcoICRI GAG'CTC Cuts at: 3034 3034 Size: 8713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2504 2561 2865 3335 3490 3500 4148 Size: 393 872 57 304 470 155 10 648 Cuts at: 4148 4481 4657 5411 5582 6776 7373 8160 8428 Size: 333 176 754 171 1194 597 787 268 Cuts at: 8428 1239 Size: 1524 Fragments arranged by size: 1524 1194 872 787 754 648 597 470 393 333 304 268 176 171 155 57 10 EcoNI CCTnn'n_nnAGG Cuts at: 4385 4672 4385 Size: 287 8426 EcoO109I rG'GnC_Cy Cuts at: 2038 2039 2180 2831 2832 2862 2863 3001 3916 Size: 1 141 651 1 30 1 138 915 Cuts at: 3916 4104 4637 4918 5655 5785 5824 5871 5959 Size: 188 533 281 737 130 39 47 88 Cuts at: 5959 2038 Size: 4792 Fragments arranged by size: 4792 915 737 651 533 281 188 141 138 130 88 47 39 30 1 1 1 EcoRI G'AATT_C Cuts at: 6022 6400 6022 Size: 378 8335 FspI TGC'GCA Cuts at: 2237 7219 2237 Size: 4982 3731 HaeII r_GCGC'y Cuts at: 1678 2016 2069 2102 2168 2270 2448 2666 2898 Size: 338 53 33 66 102 178 218 232 Cuts at: 2898 3451 3643 3868 4238 4621 4701 4837 4975 Size: 553 192 225 370 383 80 136 138 Cuts at: 4975 5073 5177 5437 5630 7860 1678 Size: 98 104 260 193 2230 2531 Fragments arranged by size: 2531 2230 553 383 370 338 260 232 225 218 193 192 178 138 136 104 102 98 80 66 53 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2309 2341 2387 2419 2543 Size: 32 135 32 737 32 46 32 124 Cuts at: 2543 2575 3366 3398 4058 4090 5816 5848 7354 Size: 32 791 32 660 32 1726 32 1506 Cuts at: 7354 7386 7428 7460 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1726 1506 791 737 660 135 124 46 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5470 6057 6303 669 Size: 988 281 3532 587 246 3079 Fragments arranged by size: 3532 3079 988 587 281 246 HindIII A'AGCT_T Cuts at: 748 4325 748 Size: 3577 5136 HpaI GTT'AAC Cuts at: 6303 6303 Size: 8713 KasI G'GCGC_C Cuts at: 2012 2098 2444 2894 4617 4833 4971 5069 5173 Size: 86 346 450 1723 216 138 98 104 Cuts at: 5173 5626 2012 Size: 453 5099 Fragments arranged by size: 5099 1723 453 450 346 216 138 104 98 86 KpnI G_GTAC'C Cuts at: 6038 6038 Size: 8713 MfeI C'AATT_G Cuts at: 6312 6312 Size: 8713 MluI A'CGCG_T Cuts at: 8589 8589 Size: 8713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5322 7827 8011 1305 Size: 4017 2505 184 2007 Fragments arranged by size: 4017 2505 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2592 2987 3308 3611 3854 Size: 54 1425 343 760 395 321 303 243 Cuts at: 3854 4568 10 Size: 714 4155 Fragments arranged by size: 4155 1425 760 714 395 343 321 303 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2618 6671 7030 7189 518 Size: 648 1452 4053 359 159 2042 Fragments arranged by size: 4053 2042 1452 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2494 2658 2783 2839 2969 3072 3213 Size: 422 466 164 125 56 130 103 141 Cuts at: 3213 3247 3360 4398 4689 4774 4830 4957 4978 Size: 34 113 1038 291 85 56 127 21 Cuts at: 4978 5114 5523 5592 5679 5692 5713 5774 5923 Size: 136 409 69 87 13 21 61 149 Cuts at: 5923 6129 6806 7954 8199 1606 Size: 206 677 1148 245 2120 Fragments arranged by size: 2120 1148 1038 677 466 422 409 291 245 206 164 149 141 136 130 127 125 113 103 87 85 69 61 56 56 34 21 21 13 NaeI GCC'GGC Cuts at: 4779 4989 5068 5172 4779 Size: 210 79 104 8320 Fragments arranged by size: 8320 210 104 79 NarI GG'CG_CC Cuts at: 2013 2099 2445 2895 4618 4834 4972 5070 5174 Size: 86 346 450 1723 216 138 98 104 Cuts at: 5174 5627 2013 Size: 453 5099 Fragments arranged by size: 5099 1723 453 450 346 216 138 104 98 86 NcoI C'CATG_G Cuts at: 513 1065 4569 513 Size: 552 3504 4657 Fragments arranged by size: 4657 3504 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8713 NgoMIV G'CCGG_C Cuts at: 4777 4987 5066 5170 4777 Size: 210 79 104 8320 Fragments arranged by size: 8320 210 104 79 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8713 NotI GC'GGCC_GC Cuts at: 2150 6160 2150 Size: 4010 4703 NspI r_CATG'y Cuts at: 1132 1229 4192 4558 4684 6071 7616 1132 Size: 97 2963 366 126 1387 1545 2229 Fragments arranged by size: 2963 2229 1545 1387 366 126 97 PasI CC'CwG_GG Cuts at: 1348 2678 5643 1348 Size: 1330 2965 4418 Fragments arranged by size: 4418 2965 1330 PciI A'CATG_T Cuts at: 7612 7612 Size: 8713 PflMI CCAn_nnn'nTGG Cuts at: 1108 4651 1108 Size: 3543 5170 PfoI T'CCnGG_A Cuts at: 2088 4050 2088 Size: 1962 6751 PmeI GTTT'AAAC Cuts at: 6017 6017 Size: 8713 PmlI CAC'GTG Cuts at: 3108 3108 Size: 8713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2756 2788 6782 6814 8295 8327 2756 Size: 32 3994 32 1481 32 3142 Fragments arranged by size: 3994 3142 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5785 5824 5959 5785 Size: 39 135 8539 Fragments arranged by size: 8539 135 39 PshAI GACnn'nnGTC Cuts at: 1613 2634 1613 Size: 1021 7692 PsiI TTA'TAA Cuts at: 6283 6283 Size: 8713 PspOMI G'GGCC_C Cuts at: 2038 2123 2180 2212 2831 2862 3915 4637 4787 Size: 85 57 32 619 31 1053 722 150 Cuts at: 4787 5654 5673 5870 2038 Size: 867 19 197 4881 Fragments arranged by size: 4881 1053 867 722 619 197 150 85 57 32 31 19 PspXI vC'TCGA_Gb Cuts at: 3737 5819 5999 3737 Size: 2082 180 6451 Fragments arranged by size: 6451 2082 180 PstI C_TGCA'G Cuts at: 830 2310 2493 3276 3349 4288 4431 4697 6065 Size: 1480 183 783 73 939 143 266 1368 Cuts at: 6065 830 Size: 3478 Fragments arranged by size: 3478 1480 1368 939 783 266 183 143 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8713 PvuII CAG'CTG Cuts at: 2494 2783 2839 2969 3213 4689 5592 2494 Size: 289 56 130 244 1476 903 5615 Fragments arranged by size: 5615 1476 903 289 244 130 56 RsrII CG'GwC_CG Cuts at: 5525 5525 Size: 8713 SacI G_AGCT'C Cuts at: 3036 3036 Size: 8713 SacII CC_GC'GG Cuts at: 2029 2659 3073 4775 4958 5115 5693 5714 2029 Size: 630 414 1702 183 157 578 21 5028 Fragments arranged by size: 5028 1702 630 578 414 183 157 21 SalI G'TCGA_C Cuts at: 1936 5468 6055 1936 Size: 3532 587 4594 Fragments arranged by size: 4594 3532 587 SapI GCTCTTCn'nnn_ Cuts at: 2506 2747 2506 Size: 241 8472 SbfI CC_TGCA'GG Cuts at: 6065 6065 Size: 8713 ScaI AGT'ACT Cuts at: 6961 8673 6961 Size: 1712 7001 SexAI A'CCwGG_T Cuts at: 3522 4605 3522 Size: 1083 7630 SfcI C'TryA_G Cuts at: 826 1045 2306 2489 3272 3345 3745 4284 4427 Size: 219 1261 183 783 73 400 539 143 Cuts at: 4427 4693 6061 7196 7877 8068 826 Size: 266 1368 1135 681 191 1471 Fragments arranged by size: 1471 1368 1261 1135 783 681 539 400 266 219 191 183 143 73 SfiI GGCCn_nnn'nGGCC Cuts at: 2028 2037 2984 2028 Size: 9 947 7757 Fragments arranged by size: 7757 947 9 SfoI GGC'GCC Cuts at: 2014 2100 2446 2896 4619 4835 4973 5071 5175 Size: 86 346 450 1723 216 138 98 104 Cuts at: 5175 5628 2014 Size: 453 5099 Fragments arranged by size: 5099 1723 453 450 346 216 138 104 98 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8713 SgrAI Cr'CCGG_yG Cuts at: 1700 2015 5066 5170 5763 1700 Size: 315 3051 104 593 4650 Fragments arranged by size: 4650 3051 593 315 104 SmaI CCC'GGG Cuts at: 1872 2244 3956 4786 6040 1872 Size: 372 1712 830 1254 4545 Fragments arranged by size: 4545 1712 1254 830 372 SmlI C'TyrA_G Cuts at: 820 1017 1943 3236 3737 4247 5327 5819 5999 Size: 197 926 1293 501 510 1080 492 180 Cuts at: 5999 6817 7718 7980 820 Size: 818 901 262 1553 Fragments arranged by size: 1553 1293 1080 926 901 818 510 501 492 262 197 180 SnaBI TAC'GTA Cuts at: 493 6504 493 Size: 6011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8713 SphI G_CATG'C Cuts at: 1132 6071 1132 Size: 4939 3774 SrfI GCCC'GGGC Cuts at: 2244 2244 Size: 8713 SspI AAT'ATT Cuts at: 5 52 6637 5 Size: 47 6585 2081 Fragments arranged by size: 6585 2081 47 StuI AGG'CCT Cuts at: 1517 4926 1517 Size: 3409 5304 StyI C'CwwG_G Cuts at: 513 1065 2466 4569 4842 4921 6152 513 Size: 552 1401 2103 273 79 1231 3074 Fragments arranged by size: 3074 2103 1401 1231 552 273 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3080 3751 4658 5455 6740 6757 6910 Size: 1255 1500 671 907 797 1285 17 153 Cuts at: 6910 7095 7540 325 Size: 185 445 1498 Fragments arranged by size: 1500 1498 1285 1255 907 797 671 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4611 4683 6959 Size: 274 80 33 51 1443 2634 72 2276 Cuts at: 6959 8671 96 Size: 1712 138 Fragments arranged by size: 2634 2276 1712 1443 274 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4376 4408 4454 4486 5384 5416 8673 8705 4376 Size: 32 46 32 898 32 3257 32 4384 Fragments arranged by size: 4384 3257 898 46 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3380 1316 Size: 2064 6649 XbaI T'CTAG_A Cuts at: 6049 6049 Size: 8713 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3973 4648 3973 Size: 675 8038 XhoI C'TCGA_G Cuts at: 1943 3737 5327 5819 5999 1943 Size: 1794 1590 492 180 4657 Fragments arranged by size: 4657 1794 1590 492 180 XmaI C'CCGG_G Cuts at: 1870 2242 3954 4784 6038 1870 Size: 372 1712 830 1254 4545 Fragments arranged by size: 4545 1712 1254 830 372 XmnI GAAnn'nnTTC Cuts at: 6842 6842 Size: 8713 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2559 6521 276 Size: 53 83 186 936 1025 3962 2468 Fragments arranged by size: 3962 2468 1025 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI BaeI BfrBI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PsrI SanDI SwaI