################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 193 CGCG CG^CG AcsI 4 AAATTT [GA]^AATT[CT] AluI 233 AGCT AG^CT AlwNI 118 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 4 AAATTT [GA]^AATT[CT] BbvCI 91 CCTCAGC CCTCAGC(-5/-2) BglI 145 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsmAI 279 GAGAC GTCTC(1/5) Bsp1407I 326 TGTACA T^GTACA BsrBI 271 GAGCGG CCGCTC(-3/-3) BsrGI 326 TGTACA T^GTACA BstF5I 242 GGATG GGATG(2/0) BstUI 193 CGCG CG^CG BstXI 78 CCAGATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 143 GCGC GCG^C DdeI 92 CTCAG C^T[ACGT]AG DpnII 222 GATC ^GATC DraIII 60 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 192 CCGCGG C^C[GA][CT]GG EcoNI 353 CCTTACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 242 GGATG GGATG(9/13) HaeII 142 AGCGCC [GA]GCGC^[CT] HhaI 143 GCGC GCG^C HinP1I 143 GCGC G^CGC HphI 310 TCACC GGTGA(8/7) KspI 192 CCGCGG CCGC^GG MaeII 64 ACGT A^CGT MboI 222 GATC ^GATC MslI 29 CATGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 193 CGCG CG^CG NdeII 222 GATC ^GATC NspI 28 GCATGT [GA]CATG^[CT] PflMI 59 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 63 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 232 CAGCTG CAG^CTG SacII 192 CCGCGG CCGC^GG Sau3AI 222 GATC ^GATC SspBI 326 TGTACA T^GTACA Tsp509I 5 AATT ^AATT TspRI 208 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 59 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AfaI 267 GTAC GT^AC 327 GTAC ApaI 212 GGGCCC GGGCC^C 227 GGGCCC BanI 42 GGTGCC G^G[CT][GA]CC 299 GGCACC BpmI 124 CTGGAG CTGGAG(16/14) 315 CTGGAG BseRI 199 GAGGAG GAGGAG(10/8) 286 GAGGAG Cac8I 108 GCTGGC GC[ACGT]^[ACGT]GC 160 GCGGGC FauI 160 GCGGG CCCGC(4/6) 194 GCGGG PpuMI 68 GGGTCCC [GA]G^G[AT]CC[CT] 320 GGGTCCT RsaI 267 GTAC GT^AC 327 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstNI Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI Sau96I ScrFI SmaI TseI XmaCI XmaI