Restriction map for
pFN21AE6592
(Circular) (Six-base) MAPSORT of: pFN21AE6592.seq Check: 1594 from: 1 to: 5311
pFN21AE6592 5311 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2397 2654 1937
Size: 460 257 4594
Fragments arranged by size:
4594 460 257
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3374 4758 5026 1632
Size: 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 268
AfeI AGC'GCT
Cuts at: 2090 2090
Size: 5311
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 4210 5187 1203
Size: 3007 977 1327
Fragments arranged by size:
3007 1327 977
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 3054 4626 3054
Size: 1572 3739
ApaI G_GGCC'C
Cuts at: 2141 2141
Size: 5311
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2620 2846 2998 5107 1493
Size: 1127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2596 2636 1870
Size: 73 653 40 4545
Fragments arranged by size:
4545 653 73 40
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2305 2632 4087 618
Size: 325 217 627 269 249 327 1455 1842
Fragments arranged by size:
1842 1455 627 327 325 269 249 217
BanII G_rGCy'C
Cuts at: 1391 2141 2630 1391
Size: 750 489 4072
Fragments arranged by size:
4072 750 489
BbeI G_GCGC'C
Cuts at: 2309 2309
Size: 5311
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2075 2109 2224 2258 3502 3536 1903
Size: 34 138 34 115 34 1244 34 3678
Fragments arranged by size:
3678 1244 138 115 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2409 2429 2456
Size: 107 122 71 631 605 737 20 27
Cuts at: 2456 3922 136
Size: 1466 1525
Fragments arranged by size:
1525 1466 737 631 605 122 107 71
27 20
BglII A'GATC_T
Cuts at: 5306 5306
Size: 5311
BlpI GC'TnA_GC
Cuts at: 2728 2728
Size: 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2141 2291 3372 4528 1790
Size: 351 150 1081 1156 2573
Fragments arranged by size:
2573 1156 1081 351 150
BmgBI CAC'GTC
Cuts at: 1318 2374 2419 1318
Size: 1056 45 4210
Fragments arranged by size:
4210 1056 45
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3042 4000 442
Size: 416 53 83 399 24 1625 958 1753
Fragments arranged by size:
1753 1625 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3971 1239
Size: 2732 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4301 4599 3436
Size: 865 298 4148
Fragments arranged by size:
4148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2183 3974 882
Size: 617 684 1791 2219
Fragments arranged by size:
2219 1791 684 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 493
Size: 711 1898 2702
Fragments arranged by size:
2702 1898 711
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2306 3118 3500
Size: 53 83 186 936 476 297 812 382
Cuts at: 3500 4135 275
Size: 635 1451
Fragments arranged by size:
1451 936 812 635 476 382 297 186
83 53
BsaWI w'CCGG_w
Cuts at: 3744 4142 4416 4563 4974 3744
Size: 398 274 147 411 4081
Fragments arranged by size:
4081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2105 2135 1178
Size: 30 165 30 702 30 4354
Fragments arranged by size:
4354 702 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2126 2129 2291 2309 1186
Size: 937 3 3 162 18 4188
Fragments arranged by size:
4188 937 162 18 3 3
BseYI C'CCAG_C
Cuts at: 2174 4514 5010 2174
Size: 2340 496 2475
Fragments arranged by size:
2475 2340 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 2427 1163
Size: 573 543 148 4047
Fragments arranged by size:
4047 573 543 148
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 2152 2229 2382 2761 3522
Size: 275 186 72 79 77 153 379 761
Cuts at: 3522 4550 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 379 275 186 153 79
77 72
BsiHKAI G_wGCw'C
Cuts at: 3372 3457 4528 3372
Size: 85 1071 4155
Fragments arranged by size:
4155 1071 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
BsmBI CGTCTCn'nnnn_
Cuts at: 2042 2069 2042
Size: 27 5284
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2291 2630 3372 3457 4528 1391
Size: 399 351 150 339 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 351 339 150 85
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2250 2666 844
Size: 1406 416 3489
Fragments arranged by size:
3489 1406 416
BsrBI CCG'CTC
Cuts at: 1394 1823 2391 3198 1394
Size: 429 568 807 3507
Fragments arranged by size:
3507 807 568 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3955 4142 1700
Size: 110 2145 187 2869
Fragments arranged by size:
2869 2145 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 2318 2492 1925
Size: 393 174 4744
Fragments arranged by size:
4744 393 174
BssSI C'ACGA_G
Cuts at: 2252 3371 4383 2252
Size: 1119 1012 3180
Fragments arranged by size:
3180 1119 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstYI r'GATC_y
Cuts at: 1972 2641 3008 3393 3410 4862 4948 4960 5306
Size: 669 367 385 17 1452 86 12 346
Cuts at: 5306 1972
Size: 1977
Fragments arranged by size:
1977 1452 669 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2157 2184 2563 2603 513
Size: 552 939 153 27 379 40 3221
Fragments arranged by size:
3221 939 552 379 153 40 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3621 3641 2827
Size: 794 20 4497
Fragments arranged by size:
4497 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DraIII CAC_nnn'GTG
Cuts at: 2374 2482 2374
Size: 108 5203
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2149 2226 2379
Size: 54 1425 50 275 18 319 77 153
Cuts at: 2379 2421 2514 2758 3647 5276 8
Size: 42 93 244 889 1629 43
Fragments arranged by size:
1629 1425 889 319 275 244 153 93
77 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2149 2226 2379 2758 1537
Size: 275 337 77 153 379 4090
Fragments arranged by size:
4090 379 337 275 153 77
EarI CTCTTCn'nnn_
Cuts at: 1353 3241 1353
Size: 1888 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2041 2288 2360 3898 4272 4418
Size: 1064 138 452 247 72 1538 374 146
Cuts at: 4418 387
Size: 1280
Fragments arranged by size:
1538 1280 1064 452 374 247 146 138
72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3374 3971 4758 5026 1239
Size: 393 1742 597 787 268 1524
Fragments arranged by size:
1742 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2031 2129 2239 2299 2500 2031
Size: 98 110 60 201 4842
Fragments arranged by size:
4842 201 110 98 60
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 2092 2309 4458 1678
Size: 414 217 2149 2531
Fragments arranged by size:
2531 2149 414 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373
Size: 32 135 32 2380 32 42 32 2626
Fragments arranged by size:
2626 2380 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 2901 669
Size: 988 281 717 246 3079
Fragments arranged by size:
3079 988 717 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KasI G'GCGC_C
Cuts at: 2305 2305
Size: 5311
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 4425 4609 1305
Size: 780 2340 184 2007
Fragments arranged by size:
2340 2007 780 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3489
Fragments arranged by size:
3489 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3269 3628 3787 518
Size: 648 2103 359 159 2042
Fragments arranged by size:
2103 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2186 2263 2565 2605 2727 3404 4552 4797
Size: 580 77 302 40 122 677 1148 245
Cuts at: 4797 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 580 302 245 122 77
40
NarI GG'CG_CC
Cuts at: 2306 2306
Size: 5311
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3820
Fragments arranged by size:
3820 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2149 2379 2758 2149
Size: 230 379 4702
Fragments arranged by size:
4702 379 230
NspI r_CATG'y
Cuts at: 1132 1229 2669 4214 1132
Size: 97 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 97
PasI CC'CwG_GG
Cuts at: 1348 2014 1348
Size: 666 4645
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 1108
Size: 1266 4045
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2129 2239 2500 2129
Size: 110 261 4940
Fragments arranged by size:
4940 261 110
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PspOMI G'GGCC_C
Cuts at: 2137 2137
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2663 830
Size: 1833 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
SacII CC_GC'GG
Cuts at: 2187 2566 2606 2187
Size: 379 40 4892
Fragments arranged by size:
4892 379 40
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2659 3794 4475 4666 826
Size: 219 1614 1135 681 191 1471
Fragments arranged by size:
1614 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2307 2307
Size: 5311
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2598 2638 1872
Size: 726 40 4545
Fragments arranged by size:
4545 726 40
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3415 4316 4578 820
Size: 197 926 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 2282 1517
Size: 765 4546
StyI C'CwwG_G
Cuts at: 513 1065 2004 2750 513
Size: 552 939 746 3074
Fragments arranged by size:
3074 939 746 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2087 2207 2594 3338 3355 3508 3693
Size: 1255 507 120 387 744 17 153 185
Cuts at: 3693 4138 325
Size: 445 1498
Fragments arranged by size:
1498 1255 744 507 445 387 185 153
120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2596 2636 1870
Size: 726 40 4545
Fragments arranged by size:
4545 726 40
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SrfI SwaI XcmI