Restriction map for
pFN21AB5830
(Linear) (Six-base) MAPSORT of: KIBB5830.seq Check: 7670 from: 1 to: 1443
KIBB5830.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 0 815 1301 1443
Size: 815 486 142
Fragments arranged by size:
815 486 142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 664 1443
Size: 106 558 779
Fragments arranged by size:
779 558 106
AflII C'TTAA_G
Cuts at: 0 1153 1443
Size: 1153 290
AleI CACnn'nnGTG
Cuts at: 0 41 1443
Size: 41 1402
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1204 1236 1443
Size: 1204 32 207
Fragments arranged by size:
1204 207 32
ApaI G_GGCC'C
Cuts at: 0 342 756 1190 1443
Size: 342 414 434 253
Fragments arranged by size:
434 414 342 253
ApoI r'AATT_y
Cuts at: 0 1324 1443
Size: 1324 119
AvaI C'yCGr_G
Cuts at: 0 124 725 1123 1443
Size: 124 601 398 320
Fragments arranged by size:
601 398 320 124
BanI G'GyrC_C
Cuts at: 0 623 951 1443
Size: 623 328 492
Fragments arranged by size:
623 492 328
BanII G_rGCy'C
Cuts at: 0 342 756 1082 1190 1443
Size: 342 414 326 108 253
Fragments arranged by size:
414 342 326 253 108
BbeI G_GCGC'C
Cuts at: 0 955 1443
Size: 955 488
BbsI GAAGACnn'nnnn_
Cuts at: 0 196 729 1443
Size: 196 533 714
Fragments arranged by size:
714 533 196
BbvCI CC'TCA_GC
Cuts at: 0 1228 1443
Size: 1228 215
BclI T'GATC_A
Cuts at: 0 135 1443
Size: 135 1308
BlpI GC'TnA_GC
Cuts at: 0 1117 1443
Size: 1117 326
Bme1580I G_kGCm'C
Cuts at: 0 342 756 1190 1443
Size: 342 414 434 253
Fragments arranged by size:
434 414 342 253
BmgBI CAC'GTC
Cuts at: 0 810 1248 1292 1443
Size: 810 438 44 151
Fragments arranged by size:
810 438 151 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 374 1329 1407 1443
Size: 374 955 78 36
Fragments arranged by size:
955 374 78 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 795 1443
Size: 795 648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 260 595 985 1405 1443
Size: 84 176 335 390 420 38
Fragments arranged by size:
420 390 335 176 84 38
Bpu10I CC'TnA_GC
Cuts at: 0 1228 1443
Size: 1228 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 808 1443
Size: 313 495 635
Fragments arranged by size:
635 495 313
BsaAI yAC'GTr
Cuts at: 0 267 1443
Size: 267 1176
BsaHI Gr'CG_yC
Cuts at: 0 952 1443
Size: 952 491
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 1204 1234 1443
Size: 148 30 1026 30 209
Fragments arranged by size:
1026 209 148 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 378 415 760 1443
Size: 304 74 37 345 683
Fragments arranged by size:
683 345 304 74 37
BseYI C'CCAG_C
Cuts at: 0 1005 1144 1189 1443
Size: 1005 139 45 254
Fragments arranged by size:
1005 254 139 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1443
Size: 788 655
BsiEI CG_ry'CG
Cuts at: 0 669 1038 1443
Size: 669 369 405
Fragments arranged by size:
669 405 369
BsiHKAI G_wGCw'C
Cuts at: 0 1082 1217 1443
Size: 1082 135 226
Fragments arranged by size:
1082 226 135
BsmI GAATG_Cn'
Cuts at: 0 181 1443
Size: 181 1262
Bsp1286I G_dGCh'C
Cuts at: 0 342 756 1082 1190 1217 1443
Size: 342 414 326 108 27 226
Fragments arranged by size:
414 342 326 226 108 27
BsrBI CCG'CTC
Cuts at: 0 750 1443
Size: 750 693
BsrDI GCAATG_nn'
Cuts at: 0 563 1443
Size: 563 880
BssSI C'ACGA_G
Cuts at: 0 1063 1443
Size: 1063 380
BstXI CCAn_nnnn'nTGG
Cuts at: 0 43 312 1443
Size: 43 269 1131
Fragments arranged by size:
1131 269 43
BstYI r'GATC_y
Cuts at: 0 542 1097 1443
Size: 542 555 346
Fragments arranged by size:
555 542 346
BstZ17I GTA'TAC
Cuts at: 0 816 1443
Size: 816 627
Bsu36I CC'TnA_GG
Cuts at: 0 956 1283 1443
Size: 956 327 160
Fragments arranged by size:
956 327 160
BtgI C'CryG_G
Cuts at: 0 70 356 989 1443
Size: 70 286 633 454
Fragments arranged by size:
633 454 286 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1208 1443
Size: 1208 235
BtsI GCAGTG_nn'
Cuts at: 0 241 635 1443
Size: 241 394 808
Fragments arranged by size:
808 394 241
EaeI y'GGCC_r
Cuts at: 0 522 858 981 1035 1427 1443
Size: 522 336 123 54 392 16
Fragments arranged by size:
522 392 336 123 54 16
EagI C'GGCC_G
Cuts at: 0 1035 1443
Size: 1035 408
EarI CTCTTCn'nnn_
Cuts at: 0 82 1078 1088 1443
Size: 82 996 10 355
Fragments arranged by size:
996 355 82 10
EcoICRI GAG'CTC
Cuts at: 0 1080 1443
Size: 1080 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 106 260 595 664 985 1405 1443
Size: 84 22 154 335 69 321 420 38
Fragments arranged by size:
420 335 321 154 84 69 38 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 475 550 1443
Size: 475 75 893
Fragments arranged by size:
893 475 75
EcoO109I rG'GnC_Cy
Cuts at: 0 129 257 339 351 606 935 1161 1186
Size: 129 128 82 12 255 329 226 25
Cuts at: 1186 1443
Size: 257
Fragments arranged by size:
329 257 255 226 129 128 82 25
12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 501 669 1443
Size: 501 168 774
Fragments arranged by size:
774 501 168
HaeII r_GCGC'y
Cuts at: 0 955 1071 1443
Size: 955 116 372
Fragments arranged by size:
955 372 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1443
Size: 1117 32 294
Fragments arranged by size:
1117 294 32
HincII GTy'rAC
Cuts at: 0 1302 1443
Size: 1302 141
KasI G'GCGC_C
Cuts at: 0 951 1443
Size: 951 492
MscI TGG'CCA
Cuts at: 0 860 1443
Size: 860 583
MslI CAynn'nnrTG
Cuts at: 0 41 310 502 1087 1443
Size: 41 269 192 585 356
Fragments arranged by size:
585 356 269 192 41
MspA1I CmG'CkG
Cuts at: 0 183 336 1398 1443
Size: 183 153 1062 45
Fragments arranged by size:
1062 183 153 45
NarI GG'CG_CC
Cuts at: 0 952 1443
Size: 952 491
NcoI C'CATG_G
Cuts at: 0 356 1443
Size: 356 1087
NotI GC'GGCC_GC
Cuts at: 0 1035 1443
Size: 1035 408
PmlI CAC'GTG
Cuts at: 0 267 1443
Size: 267 1176
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1204 1236 1443
Size: 1204 32 207
Fragments arranged by size:
1204 207 32
PpuMI rG'GwC_Cy
Cuts at: 0 257 351 606 935 1161 1443
Size: 257 94 255 329 226 282
Fragments arranged by size:
329 282 257 255 226 94
PshAI GACnn'nnGTC
Cuts at: 0 244 1443
Size: 244 1199
PspOMI G'GGCC_C
Cuts at: 0 338 752 1186 1443
Size: 338 414 434 257
Fragments arranged by size:
434 414 338 257
PstI C_TGCA'G
Cuts at: 0 236 1443
Size: 236 1207
PvuII CAG'CTG
Cuts at: 0 183 1443
Size: 183 1260
SacI G_AGCT'C
Cuts at: 0 1082 1443
Size: 1082 361
SalI G'TCGA_C
Cuts at: 0 1300 1443
Size: 1300 143
SanDI GG'GwC_CC
Cuts at: 0 935 1443
Size: 935 508
SapI GCTCTTCn'nnn_
Cuts at: 0 82 1443
Size: 82 1361
ScaI AGT'ACT
Cuts at: 0 793 1443
Size: 793 650
SfcI C'TryA_G
Cuts at: 0 232 1443
Size: 232 1211
SfoI GGC'GCC
Cuts at: 0 953 1443
Size: 953 490
SmaI CCC'GGG
Cuts at: 0 727 1443
Size: 727 716
SmlI C'TyrA_G
Cuts at: 0 328 787 1153 1443
Size: 328 459 366 290
Fragments arranged by size:
459 366 328 290
StyI C'CwwG_G
Cuts at: 0 356 471 569 1199 1443
Size: 356 115 98 630 244
Fragments arranged by size:
630 356 244 115 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 997 1443
Size: 208 789 446
Fragments arranged by size:
789 446 208
TatI w'GTAC_w
Cuts at: 0 791 1443
Size: 791 652
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 352 384 1443
Size: 62 32 258 32 1059
Fragments arranged by size:
1059 258 62 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1249 1443
Size: 1249 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1206 1443
Size: 1206 237
XmaI C'CCGG_G
Cuts at: 0 725 1443
Size: 725 718
XmnI GAAnn'nnTTC
Cuts at: 0 451 925 1443
Size: 451 474 518
Fragments arranged by size:
518 474 451
Enzymes that do cut:
AccI AcuI AflII AleI AloI ApaI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BclI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BsrBI BsrDI BssSI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KasI MscI MslI
MspA1I NarI NcoI NotI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuII SacI SalI SanDI SapI ScaI
SfcI SfoI SmaI SmlI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BfrBI BglI BglII BmtI BsaI
BsaBI BsaWI BsiWI BsmBI BspEI BspHI BspMI BsrFI
BsrGI BssHII BstAPI BstBI BstEII ClaI CspCI DraI
DraIII DrdI EciI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PsiI PspXI PsrI PvuI RsrII
SacII SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XhoI ZraI