(Linear) (Six-base) MAPSORT of: KIEE4569.seq Check: 1198 from: 1 to: 2172 KIEE4569.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AatII G_ACGT'C Cuts at: 0 284 2172 Size: 284 1888 AccI GT'mk_AC Cuts at: 0 1442 2172 Size: 1442 730 Acc65I G'GTAC_C Cuts at: 0 1068 2172 Size: 1068 1104 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1476 1493 2172 Size: 1476 17 679 Fragments arranged by size: 1476 679 17 AfeI AGC'GCT Cuts at: 0 580 2172 Size: 580 1592 AflIII A'CryG_T Cuts at: 0 110 1406 1454 2172 Size: 110 1296 48 718 Fragments arranged by size: 1296 718 110 48 AhdI GACnn_n'nnGTC Cuts at: 0 552 1881 1938 2172 Size: 552 1329 57 234 Fragments arranged by size: 1329 552 234 57 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 64 96 2172 Size: 64 32 2076 Fragments arranged by size: 2076 64 32 ApaI G_GGCC'C Cuts at: 0 2008 2172 Size: 2008 164 AvaI C'yCGr_G Cuts at: 0 314 339 500 1232 1837 2172 Size: 314 25 161 732 605 335 Fragments arranged by size: 732 605 335 314 161 25 AvrII C'CTAG_G Cuts at: 0 1063 2172 Size: 1063 1109 BamHI G'GATC_C Cuts at: 0 689 1037 2172 Size: 689 348 1135 Fragments arranged by size: 1135 689 348 BanI G'GyrC_C Cuts at: 0 1068 1251 1359 2172 Size: 1068 183 108 813 Fragments arranged by size: 1068 813 183 108 BanII G_rGCy'C Cuts at: 0 508 2008 2172 Size: 508 1500 164 Fragments arranged by size: 1500 508 164 BbsI GAAGACnn'nnnn_ Cuts at: 0 1817 2064 2172 Size: 1817 247 108 Fragments arranged by size: 1817 247 108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 826 860 1408 1442 2172 Size: 826 34 548 34 730 Fragments arranged by size: 826 730 548 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 248 533 593 2172 Size: 248 285 60 1579 Fragments arranged by size: 1579 285 248 60 Bme1580I G_kGCm'C Cuts at: 0 788 1266 1654 2008 2172 Size: 788 478 388 354 164 Fragments arranged by size: 788 478 388 354 164 BmgBI CAC'GTC Cuts at: 0 42 1407 1455 2172 Size: 42 1365 48 717 Fragments arranged by size: 1365 717 48 42 BmrI ACTGGGnnnn_n' Cuts at: 0 1560 2172 Size: 1560 612 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1704 2172 Size: 1704 468 Bpu10I CC'TnA_GC Cuts at: 0 2009 2172 Size: 2009 163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1795 2172 Size: 1795 377 BsaI GGTCTCn'nnnn_ Cuts at: 0 448 2143 2172 Size: 448 1695 29 Fragments arranged by size: 1695 448 29 BsaAI yAC'GTr Cuts at: 0 1731 2172 Size: 1731 441 BsaHI Gr'CG_yC Cuts at: 0 281 553 830 2172 Size: 281 272 277 1342 Fragments arranged by size: 1342 281 277 272 BsaWI w'CCGG_w Cuts at: 0 840 1034 1461 2172 Size: 840 194 427 711 Fragments arranged by size: 840 711 427 194 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 64 94 742 772 1744 1774 2172 Size: 64 30 648 30 972 30 398 Fragments arranged by size: 972 648 398 64 30 30 30 BseYI C'CCAG_C Cuts at: 0 148 601 674 2172 Size: 148 453 73 1498 Fragments arranged by size: 1498 453 148 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 258 1603 2172 Size: 258 1345 569 Fragments arranged by size: 1345 569 258 BsiEI CG_ry'CG Cuts at: 0 655 999 1035 2172 Size: 655 344 36 1137 Fragments arranged by size: 1137 655 344 36 BsiHKAI G_wGCw'C Cuts at: 0 1562 2172 Size: 1562 610 BsmBI CGTCTCn'nnnn_ Cuts at: 0 821 1208 2172 Size: 821 387 964 Fragments arranged by size: 964 821 387 Bsp1286I G_dGCh'C Cuts at: 0 508 788 1266 1562 1654 2008 2172 Size: 508 280 478 296 92 354 164 Fragments arranged by size: 508 478 354 296 280 164 92 BspEI T'CCGG_A Cuts at: 0 840 1461 2172 Size: 840 621 711 Fragments arranged by size: 840 711 621 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 54 417 2117 2172 Size: 54 363 1700 55 Fragments arranged by size: 1700 363 55 54 BsrBI CCG'CTC Cuts at: 0 221 2172 Size: 221 1951 BsrDI GCAATG_nn' Cuts at: 0 2130 2172 Size: 2130 42 BsrFI r'CCGG_y Cuts at: 0 139 679 953 1964 2172 Size: 139 540 274 1011 208 Fragments arranged by size: 1011 540 274 208 139 BssHII G'CGCG_C Cuts at: 0 173 224 226 570 2172 Size: 173 51 2 344 1602 Fragments arranged by size: 1602 344 173 51 2 BssSI C'ACGA_G Cuts at: 0 613 2172 Size: 613 1559 BstAPI GCAn_nnn'nTGC Cuts at: 0 42 2172 Size: 42 2130 BstXI CCAn_nnnn'nTGG Cuts at: 0 1554 2172 Size: 1554 618 BstYI r'GATC_y Cuts at: 0 689 1037 2172 Size: 689 348 1135 Fragments arranged by size: 1135 689 348 BtgI C'CryG_G Cuts at: 0 245 1247 2172 Size: 245 1002 925 Fragments arranged by size: 1002 925 245 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 656 830 950 1019 1115 2172 Size: 656 174 120 69 96 1057 Fragments arranged by size: 1057 656 174 120 96 69 BtsI GCAGTG_nn' Cuts at: 0 168 270 1622 2172 Size: 168 102 1352 550 Fragments arranged by size: 1352 550 168 102 EaeI y'GGCC_r Cuts at: 0 996 1287 1371 1790 2018 2058 2172 Size: 996 291 84 419 228 40 114 Fragments arranged by size: 996 419 291 228 114 84 40 EagI C'GGCC_G Cuts at: 0 996 2172 Size: 996 1176 EarI CTCTTCn'nnn_ Cuts at: 0 1132 2172 Size: 1132 1040 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1171 2172 Size: 1171 1001 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1476 1493 1704 2172 Size: 1476 17 211 468 Fragments arranged by size: 1476 468 211 17 EcoO109I rG'GnC_Cy Cuts at: 0 861 1496 1834 1908 2004 2005 2172 Size: 861 635 338 74 96 1 167 Fragments arranged by size: 861 635 338 167 96 74 1 HaeII r_GCGC'y Cuts at: 0 34 582 756 2172 Size: 34 548 174 1416 Fragments arranged by size: 1416 548 174 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 538 570 1867 1899 1924 1956 2172 Size: 538 32 1297 32 25 32 216 Fragments arranged by size: 1297 538 216 32 32 32 25 HincII GTy'rAC Cuts at: 0 285 2172 Size: 285 1887 KpnI G_GTAC'C Cuts at: 0 1072 2172 Size: 1072 1100 MluI A'CGCG_T Cuts at: 0 110 2172 Size: 110 2062 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1830 1906 2172 Size: 1830 76 266 Fragments arranged by size: 1830 266 76 MscI TGG'CCA Cuts at: 0 1289 2172 Size: 1289 883 MslI CAynn'nnrTG Cuts at: 0 97 617 1987 2047 2172 Size: 97 520 1370 60 125 Fragments arranged by size: 1370 520 125 97 60 MspA1I CmG'CkG Cuts at: 0 247 323 350 377 567 888 1848 2120 Size: 247 76 27 27 190 321 960 272 Cuts at: 2120 2160 2172 Size: 40 12 Fragments arranged by size: 960 321 272 247 190 76 40 27 27 12 NaeI GCC'GGC Cuts at: 0 681 2172 Size: 681 1491 NgoMIV G'CCGG_C Cuts at: 0 679 2172 Size: 679 1493 NruI TCG'CGA Cuts at: 0 704 2172 Size: 704 1468 NspI r_CATG'y Cuts at: 0 622 2172 Size: 622 1550 PflMI CCAn_nnn'nTGG Cuts at: 0 396 2172 Size: 396 1776 PfoI T'CCnGG_A Cuts at: 0 725 2172 Size: 725 1447 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 64 96 1639 1671 2172 Size: 64 32 1543 32 501 Fragments arranged by size: 1543 501 64 32 32 PpuMI rG'GwC_Cy Cuts at: 0 861 1908 2172 Size: 861 1047 264 Fragments arranged by size: 1047 861 264 PshAI GACnn'nnGTC Cuts at: 0 1127 1241 1457 2172 Size: 1127 114 216 715 Fragments arranged by size: 1127 715 216 114 PspOMI G'GGCC_C Cuts at: 0 2004 2172 Size: 2004 168 PstI C_TGCA'G Cuts at: 0 17 38 1961 2172 Size: 17 21 1923 211 Fragments arranged by size: 1923 211 21 17 PvuII CAG'CTG Cuts at: 0 1848 2160 2172 Size: 1848 312 12 Fragments arranged by size: 1848 312 12 SacII CC_GC'GG Cuts at: 0 248 2172 Size: 248 1924 SanDI GG'GwC_CC Cuts at: 0 861 2172 Size: 861 1311 SapI GCTCTTCn'nnn_ Cuts at: 0 1132 2172 Size: 1132 1040 SfcI C'TryA_G Cuts at: 0 13 34 1957 2172 Size: 13 21 1923 215 Fragments arranged by size: 1923 215 21 13 SmaI CCC'GGG Cuts at: 0 341 502 1839 2172 Size: 341 161 1337 333 Fragments arranged by size: 1337 341 333 161 SmlI C'TyrA_G Cuts at: 0 1810 2172 Size: 1810 362 SphI G_CATG'C Cuts at: 0 622 2172 Size: 622 1550 StuI AGG'CCT Cuts at: 0 1607 2172 Size: 1607 565 StyI C'CwwG_G Cuts at: 0 413 1063 2172 Size: 413 650 1109 Fragments arranged by size: 1109 650 413 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 400 1542 2172 Size: 400 1142 630 Fragments arranged by size: 1142 630 400 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 681 713 742 774 2172 Size: 681 32 29 32 1398 Fragments arranged by size: 1398 681 32 32 29 Tth111I GACn'n_nGTC Cuts at: 0 628 859 1327 2172 Size: 628 231 468 845 Fragments arranged by size: 845 628 468 231 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1728 2172 Size: 1728 444 XmaI C'CCGG_G Cuts at: 0 339 500 1837 2172 Size: 339 161 1337 335 Fragments arranged by size: 1337 339 335 161 XmnI GAAnn'nnTTC Cuts at: 0 1777 2172 Size: 1777 395 ZraI GAC'GTC Cuts at: 0 282 2172 Size: 282 1890 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AflIII AhdI AloI ApaI AvaI AvrII BamHI BanI BanII BbsI BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII KpnI MluI MmeI MscI MslI MspA1I NaeI NgoMIV NruI NspI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacII SanDI SapI SfcI SmaI SmlI SphI StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AclI AflII AgeI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI BaeI BbeI BbvCI BciVI BclI BfrBI BglII BlpI BmtI BplI BsaBI BseRI BsiWI BsmI BspHI BsrGI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI NarI NcoI NdeI NheI NotI NsiI PacI PasI PciI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI XbaI XhoI