################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI AlwI AlwNI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BseRI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI KspI MaeI MaeIII MamI MboI MfeI MflI MluI MseI MunI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 300 CGCG CG^CG AccIII 164 TCCGGA T^CCGGA AfaI 150 GTAC GT^AC Alw44I 394 GTGCAC G^TGCAC ApaI 892 GGGCCC GGGCC^C ApaLI 394 GTGCAC G^TGCAC BbeI 676 GGCGCC GGCGC^C BbsI 196 GTCTTC GAAGAC(2/6) BcnI 1006 CCCGG CC^[GC]GG BglI 472 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1113 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1113 GCTGAGC GC^T[ACGT]AGC BpuAI 196 GTCTTC GAAGAC(2/6) BsaAI 898 TACGTG [CT]AC^GT[GA] BsaWI 164 TCCGGA [AT]^CCGG[AT] BseAI 164 TCCGGA T^CCGGA BsmAI 13 GTCTC GTCTC(1/5) BsmBI 12 CGTCTC CGTCTC(1/5) BspEI 164 TCCGGA T^CCGGA BsrDI 134 GCAATG GCAATG(2/0) BsrFI 454 GCCGGC [GA]^CCGG[CT] BstAPI 836 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 300 CGCG CG^CG CelII 1113 GCTGAGC GC^T[ACGT]AGC Cfr10I 454 GCCGGC [GA]^CCGG[CT] EcoT14I 178 CCATGG C^C[AT][AT]GG KasI 676 GGCGCC G^GCGCC MroI 164 TCCGGA T^CCGGA MvnI 300 CGCG CG^CG NaeI 454 GCCGGC GCC^GGC NarI 676 GGCGCC GG^CGCC NciI 1006 CCCGG CC^[GC]GG NcoI 178 CCATGG C^CATGG NgoMIV 454 GCCGGC G^CCGGC PpuMI 461 GGGTCCT [GA]G^G[AT]CC[CT] PstI 537 CTGCAG CTGCA^G RsaI 150 GTAC GT^AC SexAI 908 ACCAGGT A^CC[AT]GGT SfoI 676 GGCGCC GGC^GCC StuI 557 AGGCCT AGG^CCT StyI 178 CCATGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcyI 676 GGCGCC G[GA]^CG[CT]C 1036 GACGCC Aor51HI 314 AGCGCT AGC^GCT 448 AGCGCT AvaII 462 GGTCC G^G[AT]CC 585 GGACC BanII 892 GGGCCC G[GA]GC[CT]^C 1096 GAGCCC BsaHI 676 GGCGCC G[GA]^CG[CT]C 1036 GACGCC BstF5I 162 CATCC GGATG(2/0) 816 GGATG EarI 322 CTCTTC CTCTTC(1/4) 510 GAAGAG Eco47III 314 AGCGCT AGC^GCT 448 AGCGCT FokI 162 CATCC GGATG(9/13) 816 GGATG Hin1I 676 GGCGCC G[GA]^CG[CT]C 1036 GACGCC Ksp632I 322 CTCTTC CTCTTC(1/4) 510 GAAGAG PflMI 275 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 588 CCACGGGCTGG Van91I 275 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 588 CCACGGGCTGG XcmI 131 CCAGCAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 872 CCACAGCAGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AluI AspHI AvaI BalI BanI BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstNI BstXI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NlaIII NlaIV PvuII Sau96I ScrFI SfaNI SfcI TseI TspRI