(Circular) (Six-base) MAPSORT of: pFN21AE9572.seq Check: 2861 from: 1 to: 5827 pFN21AE9572 5827 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2342 2375 2433 2342 Size: 33 58 5736 Fragments arranged by size: 5736 58 33 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3637 278 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 3148 3148 Size: 5827 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 2534 3890 5274 5542 1632 Size: 514 388 1356 1384 268 1917 Fragments arranged by size: 1917 1384 1356 514 388 268 AfeI AGC'GCT Cuts at: 2321 2455 2321 Size: 134 5693 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 4726 5703 1203 Size: 3523 977 1327 Fragments arranged by size: 3523 1327 977 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AlwNI CAG_nnn'CTG Cuts at: 3570 5142 3570 Size: 1572 4255 ApaI G_GGCC'C Cuts at: 2901 2901 Size: 5827 ApaLI G'TGCA_C Cuts at: 2399 3884 5040 2399 Size: 1485 1156 3186 Fragments arranged by size: 3186 1485 1156 ApoI r'AATT_y Cuts at: 1493 3136 3362 3514 5623 1493 Size: 1643 226 152 2109 1697 Fragments arranged by size: 2109 1697 1643 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 2020 2558 2687 2851 3152 1870 Size: 73 77 538 129 164 301 4545 Fragments arranged by size: 4545 538 301 164 129 77 73 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2024 2187 2681 3148 4603 Size: 325 217 627 237 163 494 467 1455 Cuts at: 4603 618 Size: 1842 Fragments arranged by size: 1842 1455 627 494 467 325 237 217 163 BanII G_rGCy'C Cuts at: 1391 2901 3105 3146 1391 Size: 1510 204 41 4072 Fragments arranged by size: 4072 1510 204 41 BbeI G_GCGC'C Cuts at: 2685 2685 Size: 5827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2194 928 Size: 971 295 4561 Fragments arranged by size: 4561 971 295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4018 4052 1903 Size: 34 2081 34 3678 Fragments arranged by size: 3678 2081 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2483 4438 136 Size: 107 122 71 631 605 811 1955 1525 Fragments arranged by size: 1955 1525 811 631 605 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3119 3244 3119 Size: 125 5702 Bme1580I G_kGCm'C Cuts at: 1790 2403 2793 2901 2982 3888 5044 1790 Size: 613 390 108 81 906 1156 2573 Fragments arranged by size: 2573 1156 906 613 390 108 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3100 3558 4516 Size: 416 53 83 399 24 1683 458 958 Cuts at: 4516 442 Size: 1753 Fragments arranged by size: 1753 1683 958 458 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2857 2909 4487 1239 Size: 1097 521 52 1578 2579 Fragments arranged by size: 2579 1578 1097 521 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3952 4817 5115 3952 Size: 865 298 4664 Fragments arranged by size: 4664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4490 882 Size: 617 2991 2219 Fragments arranged by size: 2991 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2905 3618 493 Size: 711 1701 713 2702 Fragments arranged by size: 2702 1701 713 711 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2682 3042 3634 4016 Size: 53 83 186 936 1149 360 592 382 Cuts at: 4016 4651 275 Size: 635 1451 Fragments arranged by size: 1451 1149 936 635 592 382 360 186 83 53 BsaWI w'CCGG_w Cuts at: 2169 4260 4658 4932 5079 5490 2169 Size: 2091 398 274 147 411 2506 Fragments arranged by size: 2506 2091 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2291 2321 2765 2795 1178 Size: 30 165 30 888 30 444 30 4210 Fragments arranged by size: 4210 888 444 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5827 BseYI C'CCAG_C Cuts at: 2344 2463 2893 2981 5030 5526 2344 Size: 119 430 88 2049 496 2645 Fragments arranged by size: 2645 2049 496 430 119 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5254 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3277 4038 5066 1540 Size: 275 186 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2403 2814 2843 3053 3888 3973 5044 2403 Size: 411 29 210 835 85 1071 3186 Fragments arranged by size: 3186 1071 835 411 210 85 29 BsmI GAATG_Cn' Cuts at: 3338 3431 3338 Size: 93 5734 BsmBI CGTCTCn'nnnn_ Cuts at: 2023 2023 Size: 5827 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2403 2793 2814 2843 2901 2982 3053 Size: 399 613 390 21 29 58 81 71 Cuts at: 3053 3105 3146 3888 3973 5044 1391 Size: 52 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 613 399 390 85 81 71 58 52 41 29 21 BspEI T'CCGG_A Cuts at: 2169 5490 2169 Size: 3321 2506 BspHI T'CATG_A Cuts at: 3716 5446 3716 Size: 1730 4097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2238 2342 2349 2375 2433 3182 844 Size: 1394 104 7 26 58 749 3489 Fragments arranged by size: 3489 1394 749 104 58 26 7 BsrBI CCG'CTC Cuts at: 1394 1823 3714 1394 Size: 429 1891 3507 Fragments arranged by size: 3507 1891 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2146 4322 4496 65 Size: 1183 898 2176 174 1396 Fragments arranged by size: 2176 1396 1183 898 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2459 4471 4658 1700 Size: 110 649 2012 187 2869 Fragments arranged by size: 2869 2012 649 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 3887 4899 3887 Size: 1012 4815 BstAPI GCAn_nnn'nTGC Cuts at: 794 2847 794 Size: 2053 3774 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstXI CCAn_nnnn'nTGG Cuts at: 2050 2110 2764 2050 Size: 60 654 5113 Fragments arranged by size: 5113 654 60 BstYI r'GATC_y Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822 Size: 1185 367 385 17 1452 86 12 346 Cuts at: 5822 1972 Size: 1977 Fragments arranged by size: 1977 1452 1185 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2130 2183 2196 2593 513 Size: 552 939 126 53 13 397 3747 Fragments arranged by size: 3747 939 552 397 126 53 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 2982 3004 3343 4137 4157 2982 Size: 22 339 794 20 4652 Fragments arranged by size: 4652 794 339 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2056 2091 529 Size: 35 1492 35 4265 Fragments arranged by size: 4265 1492 35 35 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2133 2259 Size: 54 1425 50 275 18 297 6 126 Cuts at: 2259 2472 2484 2550 2724 2754 2970 3055 3274 Size: 213 12 66 174 30 216 85 219 Cuts at: 3274 4163 5792 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 297 275 219 216 213 174 126 85 66 54 50 43 30 18 12 6 EagI C'GGCC_G Cuts at: 1537 1812 3274 1537 Size: 275 1462 4090 Fragments arranged by size: 4090 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 2510 3757 1353 Size: 980 177 1247 3423 Fragments arranged by size: 3423 1247 980 177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4414 4788 4934 387 Size: 1064 138 2825 374 146 1280 Fragments arranged by size: 2825 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2336 2534 2857 2909 3890 4487 Size: 393 514 190 198 323 52 981 597 Cuts at: 4487 5274 5542 1239 Size: 787 268 1524 Fragments arranged by size: 1524 981 787 597 514 393 323 268 198 190 52 EcoO109I rG'GnC_Cy Cuts at: 2467 2897 2898 2926 2467 Size: 430 1 28 5368 Fragments arranged by size: 5368 430 28 1 EcoRI G'AATT_C Cuts at: 3136 3514 3136 Size: 378 5449 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 2182 2293 2323 2457 2685 2836 4974 1678 Size: 504 111 30 134 228 151 2138 2531 Fragments arranged by size: 2531 2138 504 228 151 134 111 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3092 3124 4468 4500 4542 Size: 32 135 32 1520 32 1344 32 42 Cuts at: 4542 4574 1373 Size: 32 2626 Fragments arranged by size: 2626 1520 1344 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3171 3417 669 Size: 988 281 1233 246 3079 Fragments arranged by size: 3079 1233 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KasI G'GCGC_C Cuts at: 2681 2681 Size: 5827 KpnI G_GTAC'C Cuts at: 3152 3152 Size: 5827 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4941 5125 1305 Size: 3636 184 2007 Fragments arranged by size: 3636 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 2486 2552 2756 3057 Size: 54 1425 343 303 351 66 204 301 Cuts at: 3057 10 Size: 2780 Fragments arranged by size: 2780 1425 351 343 303 301 204 66 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2720 2807 3785 4144 4303 518 Size: 648 942 612 87 978 359 159 2042 Fragments arranged by size: 2042 978 942 648 612 359 159 87 MspA1I CmG'CkG Cuts at: 1606 2057 2215 2257 2588 2656 2847 3027 3243 Size: 451 158 42 331 68 191 180 216 Cuts at: 3243 3920 5068 5313 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 451 331 245 216 191 180 158 68 42 NaeI GCC'GGC Cuts at: 2461 2461 Size: 5827 NarI GG'CG_CC Cuts at: 2682 2682 Size: 5827 NcoI C'CATG_G Cuts at: 513 1065 2004 2183 513 Size: 552 939 179 4157 Fragments arranged by size: 4157 939 552 179 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NgoMIV G'CCGG_C Cuts at: 2459 2459 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 3185 4730 1132 Size: 97 1956 1545 2229 Fragments arranged by size: 2229 1956 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5827 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 2286 2599 1108 Size: 1178 313 4336 Fragments arranged by size: 4336 1178 313 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2467 2467 Size: 5827 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5827 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PspOMI G'GGCC_C Cuts at: 2897 2897 Size: 5827 PstI C_TGCA'G Cuts at: 830 2546 3179 830 Size: 1716 633 3478 Fragments arranged by size: 3478 1716 633 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 PvuII CAG'CTG Cuts at: 2057 2215 3027 2057 Size: 158 812 4857 Fragments arranged by size: 4857 812 158 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SexAI A'CCwGG_T Cuts at: 2913 2913 Size: 5827 SfcI C'TryA_G Cuts at: 826 1045 2542 2608 3016 3175 4310 4991 5182 Size: 219 1497 66 408 159 1135 681 191 Cuts at: 5182 826 Size: 1471 Fragments arranged by size: 1497 1471 1135 681 408 219 191 159 66 SfoI GGC'GCC Cuts at: 2683 2683 Size: 5827 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 3154 1872 Size: 1282 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3931 4832 5094 820 Size: 197 926 1988 901 262 1553 Fragments arranged by size: 1988 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 3185 1132 Size: 2053 3774 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 2564 1517 Size: 1047 4780 StyI C'CwwG_G Cuts at: 513 1065 2004 2183 3266 513 Size: 552 939 179 1083 3074 Fragments arranged by size: 3074 1083 939 552 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3854 3871 4024 4209 4654 325 Size: 1255 2274 17 153 185 445 1498 Fragments arranged by size: 2274 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4073 5785 96 Size: 274 80 33 51 1443 2096 1712 138 Fragments arranged by size: 2096 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2081 2113 2291 2323 5787 5819 2081 Size: 32 178 32 3464 32 2089 Fragments arranged by size: 3464 2089 178 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5827 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2143 2884 2143 Size: 741 5086 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 3152 1870 Size: 1282 4545 XmnI GAAnn'nnTTC Cuts at: 3956 3956 Size: 5827 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3635 276 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI