Restriction map for pFN21AE9572
 (Linear) (Six-base) MAPSORT of: KIEE9572.seq  Check: 3034  from: 1  to: 1122



KIEE9572.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:42  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     337     370     428    1122 
   Size:        337      33      58     694

  Fragments arranged by size: 

                 694     337      58      33
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     529    1122 
   Size:        141     388     593

  Fragments arranged by size: 

                 593     388     141
 
AfeI AGC'GCT

Cuts at:      0     316     450    1122 
   Size:        316     134     672

  Fragments arranged by size: 

                 672     316     134
 
ApaI G_GGCC'C

Cuts at:      0     896    1122 
   Size:        896     226
 
ApaLI G'TGCA_C

Cuts at:      0     394    1122 
   Size:        394     728
 
AvaI C'yCGr_G

Cuts at:      0      15     553     682     846    1122 
   Size:         15     538     129     164     276

  Fragments arranged by size: 

                 538     276     164     129      15
 
BanI G'GyrC_C

Cuts at:      0      19     182     676    1122 
   Size:         19     163     494     446

  Fragments arranged by size: 

                 494     446     163      19
 
BanII G_rGCy'C

Cuts at:      0     896    1100    1122 
   Size:        896     204      22

  Fragments arranged by size: 

                 896     204      22
 
BbeI G_GCGC'C

Cuts at:      0     680    1122 
   Size:        680     442
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     189    1122 
   Size:        189     933
 
BglI GCCn_nnn'nGGC

Cuts at:      0     478    1122 
   Size:        478     644
 
BlpI GC'TnA_GC

Cuts at:      0    1114    1122 
   Size:       1114       8
 
Bme1580I G_kGCm'C

Cuts at:      0     398     788     896     977    1122 
   Size:        398     390     108      81     145

  Fragments arranged by size: 

                 398     390     145     108      81
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1095    1122 
   Size:       1095      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     852     904    1122 
   Size:        331     521      52     218

  Fragments arranged by size: 

                 521     331     218      52
 
BsaAI yAC'GTr

Cuts at:      0     900    1122 
   Size:        900     222
 
BsaHI Gr'CG_yC

Cuts at:      0     677    1037    1122 
   Size:        677     360      85

  Fragments arranged by size: 

                 677     360      85
 
BsaWI w'CCGG_w

Cuts at:      0     164    1122 
   Size:        164     958
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     286     316     760     790    1122 
   Size:        286      30     444      30     332

  Fragments arranged by size: 

                 444     332     286      30      30
 
BseYI C'CCAG_C

Cuts at:      0     339     458     888     976    1122 
   Size:        339     119     430      88     146

  Fragments arranged by size: 

                 430     339     146     119      88
 
BsiHKAI G_wGCw'C

Cuts at:      0     398     809     838    1048    1122 
   Size:        398     411      29     210      74

  Fragments arranged by size: 

                 411     398     210      74      29
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      18    1122 
   Size:         18    1104
 
Bsp1286I G_dGCh'C

Cuts at:      0     398     788     809     838     896     977    1048    1100 
   Size:        398     390      21      29      58      81      71      52

Cuts at:   1100    1122 
   Size:         22

  Fragments arranged by size: 

                 398     390      81      71      58      52      29      22
                  21
 
BspEI T'CCGG_A

Cuts at:      0     164    1122 
   Size:        164     958
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     233     337     344     370     428    1122 
   Size:        233     104       7      26      58     694

  Fragments arranged by size: 

                 694     233     104      58      26       7
 
BsrDI GCAATG_nn'

Cuts at:      0     141    1122 
   Size:        141     981
 
BsrFI r'CCGG_y

Cuts at:      0     454    1122 
   Size:        454     668
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     842    1122 
   Size:        842     280
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      45     105     759    1122 
   Size:         45      60     654     363

  Fragments arranged by size: 

                 654     363      60      45
 
BtgI C'CryG_G

Cuts at:      0     125     178     191     588    1122 
   Size:        125      53      13     397     534

  Fragments arranged by size: 

                 534     397     125      53      13
 
BtsI GCAGTG_nn'

Cuts at:      0     977     999    1122 
   Size:        977      22     123

  Fragments arranged by size: 

                 977     123      22
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      51      86    1122 
   Size:         51      35    1036

  Fragments arranged by size: 

                1036      51      35
 
EaeI y'GGCC_r

Cuts at:      0     122     128     254     467     479     545     719     749 
   Size:        122       6     126     213      12      66     174      30

Cuts at:    749     965    1050    1122 
   Size:        216      85      72

  Fragments arranged by size: 

                 216     213     174     126     122      85      72      66
                  30      12       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     328     505    1122 
   Size:        328     177     617

  Fragments arranged by size: 

                 617     328     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     331     529     852     904    1122 
   Size:        141     190     198     323      52     218

  Fragments arranged by size: 

                 323     218     198     190     141      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0     462     892     893     921    1122 
   Size:        462     430       1      28     201

  Fragments arranged by size: 

                 462     430     201      28       1
 
HaeII r_GCGC'y

Cuts at:      0     177     288     318     452     680     831    1122 
   Size:        177     111      30     134     228     151     291

  Fragments arranged by size: 

                 291     228     177     151     134     111      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1087    1119    1122 
   Size:       1087      32       3

  Fragments arranged by size: 

                1087      32       3
 
KasI G'GCGC_C

Cuts at:      0     676    1122 
   Size:        676     446
 
MscI TGG'CCA

Cuts at:      0     130     481     547     751    1052    1122 
   Size:        130     351      66     204     301      70

  Fragments arranged by size: 

                 351     301     204     130      70      66
 
MslI CAynn'nnrTG

Cuts at:      0     103     715     802    1122 
   Size:        103     612      87     320

  Fragments arranged by size: 

                 612     320     103      87
 
MspA1I CmG'CkG

Cuts at:      0      52     210     252     583     651     842    1022    1122 
   Size:         52     158      42     331      68     191     180     100

  Fragments arranged by size: 

                 331     191     180     158     100      68      52      42
            
 
NaeI GCC'GGC

Cuts at:      0     456    1122 
   Size:        456     666
 
NarI GG'CG_CC

Cuts at:      0     677    1122 
   Size:        677     445
 
NcoI C'CATG_G

Cuts at:      0     178    1122 
   Size:        178     944
 
NgoMIV G'CCGG_C

Cuts at:      0     454    1122 
   Size:        454     668
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     281     594    1122 
   Size:        281     313     528

  Fragments arranged by size: 

                 528     313     281
 
PpuMI rG'GwC_Cy

Cuts at:      0     462    1122 
   Size:        462     660
 
PspOMI G'GGCC_C

Cuts at:      0     892    1122 
   Size:        892     230
 
PstI C_TGCA'G

Cuts at:      0     541    1122 
   Size:        541     581
 
PvuII CAG'CTG

Cuts at:      0      52     210    1022    1122 
   Size:         52     158     812     100

  Fragments arranged by size: 

                 812     158     100      52
 
SexAI A'CCwGG_T

Cuts at:      0     908    1122 
   Size:        908     214
 
SfcI C'TryA_G

Cuts at:      0     537     603    1011    1122 
   Size:        537      66     408     111

  Fragments arranged by size: 

                 537     408     111      66
 
SfoI GGC'GCC

Cuts at:      0     678    1122 
   Size:        678     444
 
StuI AGG'CCT

Cuts at:      0     559    1122 
   Size:        559     563
 
StyI C'CwwG_G

Cuts at:      0     178    1122 
   Size:        178     944
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      76     108     286     318    1122 
   Size:         76      32     178      32     804

  Fragments arranged by size: 

                 804     178      76      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     138     879    1122 
   Size:        138     741     243

  Fragments arranged by size: 

                 741     243     138
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     ApaI    ApaLI     AvaI     BanI    BanII
     BbeI     BbsI     BglI     BlpI Bme1580I     BmrI     BpmI    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspMI    BsrDI    BsrFI   BstAPI    BstXI     BtgI     BtsI    CspCI
     EaeI     EarI  Eco57MI EcoO109I    HaeII    Hin4I     KasI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV    PflMI    PpuMI
   PspOMI     PstI    PvuII    SexAI     SfcI     SfoI     StuI     StyI
     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BspHI    BsrBI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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