################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbvCI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstAPI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FbaI FseI FspI HincII HindII HpaI KpnI KspI MaeI MfeI MluI MroI MunI NheI NotI NruI PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfcI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AfaI 2040 GTAC GT^AC AocI 106 CCTAAGG CC^T[ACGT]AGG AscI 67 GGCGCGCC GG^CGCGCC AspHI 304 GAGCTC G[AT]GC[AT]^C BbrPI 2032 CACGTG CAC^GTG BcgI 1466 GCATGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 545 GTATCC GTATCC(6/5) BsiEI 1503 CGACCG CG[GA][CT]^CG BsiHKAI 304 GAGCTC G[AT]GC[AT]^C BssHII 68 GCGCGC G^CGCGC BstBI 2171 TTCGAA TT^CGAA Bsu36I 106 CCTAAGG CC^T[ACGT]AGG Eco81I 106 CCTAAGG CC^T[ACGT]AGG EcoRI 352 GAATTC G^AATTC EcoT22I 584 ATGCAT ATGCA^T HindIII 1896 AAGCTT A^AGCTT HinfI 2098 GAGTC G^A[ACGT]TC NdeI 188 CATATG CA^TATG NsiI 584 ATGCAT ATGCA^T NspI 453 ACATGC [GA]CATG^[CT] NspV 2171 TTCGAA TT^CGAA PleI 2098 GAGTC GAGTC(4/5) PmaCI 2032 CACGTG CAC^GTG PmlI 2032 CACGTG CAC^GTG PvuII 799 CAGCTG CAG^CTG RsaI 2040 GTAC GT^AC SacI 304 GAGCTC GAGCT^C SfuI 2171 TTCGAA TT^CGAA StuI 1952 AGGCCT AGG^CCT Tsp45I 255 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 352 GAATTC [GA]^AATT[CT] 2014 AAATTC AcyI 1675 GGCGCC G[GA]^CG[CT]C 1714 GGCGCC ApoI 352 GAATTC [GA]^AATT[CT] 2014 AAATTC BbeI 1675 GGCGCC GGCGC^C 1714 GGCGCC BbsI 416 GAAGAC GAAGAC(2/6) 599 GAAGAC BbvI 382 GCTGC GCAGC(8/12) 801 GCTGC BglI 1373 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1945 GCCAGGAAGGC BpuAI 416 GAAGAC GAAGAC(2/6) 599 GAAGAC BsaAI 298 TACGTG [CT]AC^GT[GA] 2032 CACGTG BsaHI 1675 GGCGCC G[GA]^CG[CT]C 1714 GGCGCC BsrDI 1155 CATTGC GCAATG(2/0) 466 GCAATG BsrI 490 ACTGG ACTGG(1/-1) 542 CCAGT BstYI 438 AGATCC [GA]^GATC[CT] 1090 GGATCT Hin1I 1675 GGCGCC G[GA]^CG[CT]C 1714 GGCGCC KasI 1675 GGCGCC G^GCGCC 1714 GGCGCC MflI 438 AGATCC [GA]^GATC[CT] 1090 GGATCT MspA1I 799 CAGCTG C[AC]G^C[GT]G 1375 CCGCTG NaeI 1240 GCCGGC GCC^GGC 1462 GCCGGC NarI 1675 GGCGCC GG^CGCC 1714 GGCGCC NgoMIV 1240 GCCGGC G^CCGGC 1462 GCCGGC PaeR7I 1726 CTCGAG C^TCGAG 1765 CTCGAG PflMI 1272 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1419 CCAGACCATGG PpuMI 987 AGGTCCC [GA]G^G[AT]CC[CT] 1212 GGGTCCT SexAI 266 ACCTGGT A^CC[AT]GGT 510 ACCAGGT SfiI 1372 GGCCCGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1944 GGCCAGGAAGGCC SfoI 1675 GGCGCC GGC^GCC 1714 GGCGCC TseI 382 GCTGC G^C[AT]GC 801 GCTGC Van91I 1272 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1419 CCAGACCATGG XhoI 1726 CTCGAG C^TCGAG 1765 CTCGAG XhoII 438 AGATCC [GA]^GATC[CT] 1090 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AluI AlwI ApaI AvaI AvaII BalI BanI BanII BcnI BmyI BpmI BsaBI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MamI MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI Tru9I Tsp509I TspRI TthHB8I XcmI