Restriction map for pFN21AE2375
 (Linear) (Six-base) MAPSORT of: KIEE2375.seq  Check: 8797  from: 1  to: 2190



KIEE2375.orf

 With 182 enzymes: SgfI * 

                        October 20, 2011 13:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     679    1159    2190 
   Size:        341     338     480    1031

  Fragments arranged by size: 

                1031     480     341     338
 
ApaI G_GGCC'C

Cuts at:      0     106    1055    1375    1558    1636    1780    1792    2190 
   Size:        106     949     320     183      78     144      12     398

  Fragments arranged by size: 

                 949     398     320     183     144     106      78      12
            
 
ApoI r'AATT_y

Cuts at:      0     352    2014    2190 
   Size:        352    1662     176

  Fragments arranged by size: 

                1662     352     176
 
AscI GG'CGCG_CC

Cuts at:      0      68    2190 
   Size:         68    2122
 
AvaI C'yCGr_G

Cuts at:      0    1231    1726    1765    2190 
   Size:       1231     495      39     425

  Fragments arranged by size: 

                1231     495     425      39
 
BanI G'GyrC_C

Cuts at:      0    1237    1459    1596    1675    1714    1753    1846    2190 
   Size:       1237     222     137      79      39      39      93     344

  Fragments arranged by size: 

                1237     344     222     137      93      79      39      39
            
 
BanII G_rGCy'C

Cuts at:      0      99     106     308    1055    1294    1336    1354    1375 
   Size:         99       7     202     747     239      42      18      21

Cuts at:   1375    1537    1558    1636    1780    1792    2190 
   Size:        162      21      78     144      12     398

  Fragments arranged by size: 

                 747     398     239     202     162     144      99      78
                  42      21      21      18      12       7
 
BbeI G_GCGC'C

Cuts at:      0    1679    1718    2190 
   Size:       1679      39     472

  Fragments arranged by size: 

                1679     472      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     423     606    2190 
   Size:        423     183    1584

  Fragments arranged by size: 

                1584     423     183
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1455    1489    2190 
   Size:       1455      34     701

  Fragments arranged by size: 

                1455     701      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     556    2190 
   Size:        556    1634
 
BfrBI ATG'CAT

Cuts at:      0     586    2190 
   Size:        586    1604
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1379    1951    2190 
   Size:       1379     572     239

  Fragments arranged by size: 

                1379     572     239
 
Bme1580I G_kGCm'C

Cuts at:      0     106    1055    1375    1558    1601    1636    1780    1792 
   Size:        106     949     320     183      43      35     144      12

Cuts at:   1792    2190 
   Size:        398

  Fragments arranged by size: 

                 949     398     320     183     144     106      43      35
                  12
 
BmgBI CAC'GTC

Cuts at:      0     825    2190 
   Size:        825    1365
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     536    2190 
   Size:        536    1654
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1383    1642    1684    1708    1723    1762    1943    2190 
   Size:       1383     259      42      24      15      39     181     247

  Fragments arranged by size: 

                1383     259     247     181      42      39      24      15
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     872    1579    2190 
   Size:        872     707     611

  Fragments arranged by size: 

                 872     707     611
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1501    2190 
   Size:       1501     689
 
BsaAI yAC'GTr

Cuts at:      0     300    2034    2190 
   Size:        300    1734     156

  Fragments arranged by size: 

                1734     300     156
 
BsaBI GATnn'nnATC

Cuts at:      0     532     950    1089    2190 
   Size:        532     418     139    1101

  Fragments arranged by size: 

                1101     532     418     139
 
BsaHI Gr'CG_yC

Cuts at:      0    1676    1715    2190 
   Size:       1676      39     475

  Fragments arranged by size: 

                1676     475      39
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1175    1205    1381    1411    2190 
   Size:       1175      30     176      30     779

  Fragments arranged by size: 

                1175     779     176      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     182    1160    1184    2190 
   Size:        182     978      24    1006

  Fragments arranged by size: 

                1006     978     182      24
 
BseYI C'CCAG_C

Cuts at:      0    1377    2190 
   Size:       1377     813
 
BsiEI CG_ry'CG

Cuts at:      0    1506    2190 
   Size:       1506     684
 
BsiHKAI G_wGCw'C

Cuts at:      0     308    2190 
   Size:        308    1882
 
Bsp1286I G_dGCh'C

Cuts at:      0      99     106     308    1055    1294    1336    1354    1375 
   Size:         99       7     202     747     239      42      18      21

Cuts at:   1375    1537    1558    1601    1636    1780    1792    2190 
   Size:        162      21      43      35     144      12     398

  Fragments arranged by size: 

                 747     398     239     202     162     144      99      43
                  42      35      21      21      18      12       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     135    1594    1894    2190 
   Size:        135    1459     300     296

  Fragments arranged by size: 

                1459     300     296     135
 
BsrBI CCG'CTC

Cuts at:      0      65      86     413    1495    1692    2190 
   Size:         65      21     327    1082     197     498

  Fragments arranged by size: 

                1082     498     327     197      65      21
 
BsrDI GCAATG_nn'

Cuts at:      0     473    1154    2190 
   Size:        473     681    1036

  Fragments arranged by size: 

                1036     681     473
 
BsrFI r'CCGG_y

Cuts at:      0    1240    1462    1657    2190 
   Size:       1240     222     195     533

  Fragments arranged by size: 

                1240     533     222     195
 
BssHII G'CGCG_C

Cuts at:      0      68    2190 
   Size:         68    2122
 
BstBI TT'CG_AA

Cuts at:      0    2172    2190 
   Size:       2172      18
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     901    1662    1686    1872    1936    2190 
   Size:        901     761      24     186      64     254

  Fragments arranged by size: 

                 901     761     254     186      64      24
 
BstYI r'GATC_y

Cuts at:      0     438    1090    2190 
   Size:        438     652    1100

  Fragments arranged by size: 

                1100     652     438
 
Bsu36I CC'TnA_GG

Cuts at:      0     107    2190 
   Size:        107    2083
 
BtgI C'CryG_G

Cuts at:      0     894    1272    1337    1424    1784    1829    2190 
   Size:        894     378      65      87     360      45     361

  Fragments arranged by size: 

                 894     378     361     360      87      65      45
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2061    2096    2190 
   Size:       2061      35      94

  Fragments arranged by size: 

                2061      94      35
 
EaeI y'GGCC_r

Cuts at:      0     795    1490    1652    1826    1943    2028    2111    2154 
   Size:        795     695     162     174     117      85      83      43

Cuts at:   2154    2190 
   Size:         36

  Fragments arranged by size: 

                 795     695     174     162     117      85      83      43
                  36
 
EarI CTCTTCn'nnn_

Cuts at:      0      53     136     359    1135    2190 
   Size:         53      83     223     776    1055

  Fragments arranged by size: 

                1055     776     223      83      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      40    2190 
   Size:         40    2150
 
EcoICRI GAG'CTC

Cuts at:      0     306    2190 
   Size:        306    1884
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     679    1159    1383    1642    1684    1708    1723 
   Size:        341     338     480     224     259      42      24      15

Cuts at:   1723    1762    1943    2190 
   Size:         39     181     247

  Fragments arranged by size: 

                 480     341     338     259     247     224     181      42
                  39      24      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     103     842     988    1051    1052    1213    1555    1633 
   Size:        103     739     146      63       1     161     342      78

Cuts at:   1633    1777    2190 
   Size:        144     413

  Fragments arranged by size: 

                 739     413     342     161     146     144     103      78
                  63       1
 
EcoRI G'AATT_C

Cuts at:      0     352    2190 
   Size:        352    1838
 
HaeII r_GCGC'y

Cuts at:      0    1679    1718    1805    2190 
   Size:       1679      39      87     385

  Fragments arranged by size: 

                1679     385      87      39
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1136    1168    1175    1197    1207    1229    2190 
   Size:       1136      32       7      22      10      22     961

  Fragments arranged by size: 

                1136     961      32      22      22      10       7
 
HindIII A'AGCT_T

Cuts at:      0    1896    2190 
   Size:       1896     294
 
KasI G'GCGC_C

Cuts at:      0    1675    1714    2190 
   Size:       1675      39     476

  Fragments arranged by size: 

                1675     476      39
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     223    2190 
   Size:        223    1967
 
MscI TGG'CCA

Cuts at:      0     797    1654    1828    1945    2190 
   Size:        797     857     174     117     245

  Fragments arranged by size: 

                 857     797     245     174     117
 
MslI CAynn'nnrTG

Cuts at:      0     973    1870    2053    2190 
   Size:        973     897     183     137

  Fragments arranged by size: 

                 973     897     183     137
 
MspA1I CmG'CkG

Cuts at:      0     801    1377    2190 
   Size:        801     576     813

  Fragments arranged by size: 

                 813     801     576
 
NaeI GCC'GGC

Cuts at:      0    1242    1464    2190 
   Size:       1242     222     726

  Fragments arranged by size: 

                1242     726     222
 
NarI GG'CG_CC

Cuts at:      0    1676    1715    2190 
   Size:       1676      39     475

  Fragments arranged by size: 

                1676     475      39
 
NcoI C'CATG_G

Cuts at:      0     894    1424    1784    1829    2190 
   Size:        894     530     360      45     361

  Fragments arranged by size: 

                 894     530     361     360      45
 
NdeI CA'TA_TG

Cuts at:      0     189    2190 
   Size:        189    2001
 
NgoMIV G'CCGG_C

Cuts at:      0    1240    1462    2190 
   Size:       1240     222     728

  Fragments arranged by size: 

                1240     728     222
 
NsiI A_TGCA'T

Cuts at:      0     588    2190 
   Size:        588    1602
 
NspI r_CATG'y

Cuts at:      0     457    2190 
   Size:        457    1733
 
PasI CC'CwG_GG

Cuts at:      0    1195    1797    2190 
   Size:       1195     602     393

  Fragments arranged by size: 

                1195     602     393
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1278    1425    2190 
   Size:       1278     147     765

  Fragments arranged by size: 

                1278     765     147
 
PmlI CAC'GTG

Cuts at:      0    2034    2190 
   Size:       2034     156
 
PpuMI rG'GwC_Cy

Cuts at:      0     988    1213    2190 
   Size:        988     225     977

  Fragments arranged by size: 

                 988     977     225
 
PsiI TTA'TAA

Cuts at:      0     638    2190 
   Size:        638    1552
 
PspOMI G'GGCC_C

Cuts at:      0     102    1051    1371    1554    1632    1776    1788    2190 
   Size:        102     949     320     183      78     144      12     402

  Fragments arranged by size: 

                 949     402     320     183     144     102      78      12
            
 
PspXI vC'TCGA_Gb

Cuts at:      0    1726    1765    2190 
   Size:       1726      39     425

  Fragments arranged by size: 

                1726     425      39
 
PvuII CAG'CTG

Cuts at:      0     801    2190 
   Size:        801    1389
 
SacI G_AGCT'C

Cuts at:      0     308    2190 
   Size:        308    1882
 
SexAI A'CCwGG_T

Cuts at:      0     266     510    2190 
   Size:        266     244    1680

  Fragments arranged by size: 

                1680     266     244
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1379    1951    2190 
   Size:       1379     572     239

  Fragments arranged by size: 

                1379     572     239
 
SfoI GGC'GCC

Cuts at:      0    1677    1716    2190 
   Size:       1677      39     474

  Fragments arranged by size: 

                1677     474      39
 
SmlI C'TyrA_G

Cuts at:      0    1480    1726    1765    2190 
   Size:       1480     246      39     425

  Fragments arranged by size: 

                1480     425     246      39
 
StuI AGG'CCT

Cuts at:      0    1954    2190 
   Size:       1954     236
 
StyI C'CwwG_G

Cuts at:      0     894     900    1424    1784    1829    2072    2190 
   Size:        894       6     524     360      45     243     118

  Fragments arranged by size: 

                 894     524     360     243     118      45       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     555    1447    1741    1822    2190 
   Size:        555     892     294      81     368

  Fragments arranged by size: 

                 892     555     368     294      81
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     530     562    1381    1413    2190 
   Size:        530      32     819      32     777

  Fragments arranged by size: 

                 819     777     530      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     901    1506    1527    1569    2190 
   Size:        901     605      21      42     621

  Fragments arranged by size: 

                 901     621     605      42      21
 
XhoI C'TCGA_G

Cuts at:      0    1726    1765    2190 
   Size:       1726      39     425

  Fragments arranged by size: 

                1726     425      39
 

 Enzymes that do cut:

     AcuI     ApaI     ApoI     AscI     AvaI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BstBI    BstXI    BstYI   Bsu36I     BtgI    CspCI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I
  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI    PflMI     PmlI
    PpuMI     PsiI   PspOMI    PspXI    PvuII     SacI    SexAI     SfiI
     SfoI     SmlI     StuI     StyI    TaqII     TstI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BclI    BglII     BlpI
     BmtI     BplI     BsaI    BsaWI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrGI    BssSI   BstAPI   BstEII  BstZ17I    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI   HincII     HpaI     KpnI     MfeI
     MluI     NheI     NotI     NruI     PacI     PciI     PfoI     PmeI
     PpiI    PshAI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I
     XbaI     XmaI     XmnI     ZraI
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