################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanII BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MboII MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AcyI 256 GGCGCC G[GA]^CG[CT]C AlwNI 646 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 204 TGGCCA TGG^CCA BanI 256 GGCGCC G^G[CT][GA]CC BbeI 256 GGCGCC GGCGC^C BbvCI 458 CCTCAGC CCTCAGC(-5/-2) BpmI 112 CTGGAG CTGGAG(16/14) BsaHI 256 GGCGCC G[GA]^CG[CT]C BsaI 454 GAGACC GGTCTC(1/5) BsgI 248 GTGCAG GTGCAG(16/14) BsiYI 387 CCACCTGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 387 CCACCTGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 454 GAGAC GTCTC(1/5) BsmI 490 GCATTC GAATGC(1/-1) BstXI 128 CCATCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 4 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 204 TGGCCA [CT]^GGCC[GA] EcoO109I 4 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 464 CCTTGG C^C[AT][AT]GG Hin1I 256 GGCGCC G[GA]^CG[CT]C KasI 256 GGCGCC G^GCGCC MluNI 204 TGGCCA TGG^CCA MscI 204 TGGCCA TGG^CCA MspA1I 200 CCGCTG C[AC]G^C[GT]G NarI 256 GGCGCC GG^CGCC NlaIII 357 CATG CATG^ PpuMI 4 GGGACCC [GA]G^G[AT]CC[CT] PstI 634 CTGCAG CTGCA^G SfcI 634 CTGCAG C^T[GA][CT]AG SfoI 256 GGCGCC GGC^GCC StyI 464 CCTTGG C^C[AT][AT]GG TaqI 452 TCGA T^CGA TfiI 469 GATTC G^A[AT]TC TthHB8I 452 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AluI 323 AGCT AG^CT 632 AGCT Aor51HI 212 AGCGCT AGC^GCT 308 AGCGCT AspHI 43 GTGCTC G[AT]GC[AT]^C 123 GAGCAC AvaI 8 CCCGGG C^[CT]CG[GA]G 570 CCCGGG AvaII 5 GGACC G^G[AT]CC 330 GGACC BglII 188 AGATCT A^GATCT 512 AGATCT BsiHKAI 43 GTGCTC G[AT]GC[AT]^C 123 GAGCAC BsoBI 8 CCCGGG C^[CT]CG[GA]G 570 CCCGGG BstF5I 156 CATCC GGATG(2/0) 501 CATCC BstYI 188 AGATCT [GA]^GATC[CT] 512 AGATCT DdeI 57 CTCAG C^T[ACGT]AG 459 CTCAG DsaI 243 CCGTGG C^C[GA][CT]GG 608 CCACGG Eco47III 212 AGCGCT AGC^GCT 308 AGCGCT FokI 156 CATCC GGATG(9/13) 501 CATCC HaeIII 205 GGCC GG^CC 414 GGCC HapII 9 CCGG C^CGG 571 CCGG HpaII 9 CCGG C^CGG 571 CCGG HphI 92 TCACC GGTGA(8/7) 227 TCACC MaeIII 377 GTGAC ^GT[ACGT]AC 528 GTCAC MflI 188 AGATCT [GA]^GATC[CT] 512 AGATCT MspI 9 CCGG C^CGG 571 CCGG PleI 55 GACTC GAGTC(4/5) 565 GAGTC SfaNI 155 GCATC GCATC(5/9) 521 GCATC SmaI 8 CCCGGG CCC^GGG 570 CCCGGG Tsp45I 377 GTGAC ^GT[GC]AC 528 GTCAC XhoII 188 AGATCT [GA]^GATC[CT] 512 AGATCT XmaCI 8 CCCGGG C^CCGGG 570 CCCGGG XmaI 8 CCCGGG C^CCGGG 570 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AccII AciI AfaI BbvI BcnI BmyI BsaJI BsmFI Bsp1286I BspMI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII EcoRII FauI Fnu4HI HaeII HhaI HinP1I HinfI ItaI MaeII MboI MnlI MslI MvaI MvnI MwoI NciI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI TseI TspRI