Restriction map for pFN21AE1715
 (Linear) (Six-base) MAPSORT of: KIEE1715.seq  Check: 2323  from: 1  to: 654



KIEE1715.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     398     654 
   Size:        398     256
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     654 
   Size:        337     317
 
AfeI AGC'GCT

Cuts at:      0     214     310     654 
   Size:        214      96     344

  Fragments arranged by size: 

                 344     214      96
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     427     459     654 
   Size:        427      32     195

  Fragments arranged by size: 

                 427     195      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     651     654 
   Size:        651       3
 
AvaI C'yCGr_G

Cuts at:      0       8     570     654 
   Size:          8     562      84

  Fragments arranged by size: 

                 562      84       8
 
BanI G'GyrC_C

Cuts at:      0     256     654 
   Size:        256     398
 
BbeI G_GCGC'C

Cuts at:      0     260     654 
   Size:        260     394
 
BbvCI CC'TCA_GC

Cuts at:      0     459     654 
   Size:        459     195
 
BglII A'GATC_T

Cuts at:      0     188     512     654 
   Size:        188     324     142

  Fragments arranged by size: 

                 324     188     142
 
Bme1580I G_kGCm'C

Cuts at:      0     404     654 
   Size:        404     250
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     654 
   Size:        133     521
 
Bpu10I CC'TnA_GC

Cuts at:      0     459     654 
   Size:        459     195
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     448     654 
   Size:        448     206
 
BsaHI Gr'CG_yC

Cuts at:      0     257     654 
   Size:        257     397
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     654 
   Size:        269     385
 
BsiHKAI G_wGCw'C

Cuts at:      0      47     127     654 
   Size:         47      80     527

  Fragments arranged by size: 

                 527      80      47
 
BsmI GAATG_Cn'

Cuts at:      0     490     654 
   Size:        490     164
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     127     404     654 
   Size:         47      80     277     250

  Fragments arranged by size: 

                 277     250      80      47
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      86     389     398     654 
   Size:         86     303       9     256

  Fragments arranged by size: 

                 303     256      86       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     135     654 
   Size:        135     519
 
BstYI r'GATC_y

Cuts at:      0     188     512     654 
   Size:        188     324     142

  Fragments arranged by size: 

                 324     188     142
 
BtgI C'CryG_G

Cuts at:      0     243     608     654 
   Size:        243     365      46

  Fragments arranged by size: 

                 365     243      46
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     261     654 
   Size:        261     393
 
BtsI GCAGTG_nn'

Cuts at:      0     257     643     654 
   Size:        257     386      11

  Fragments arranged by size: 

                 386     257      11
 
EaeI y'GGCC_r

Cuts at:      0     204     654 
   Size:        204     450
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     337     654 
   Size:        133     204     317

  Fragments arranged by size: 

                 317     204     133
 
EcoO109I rG'GnC_Cy

Cuts at:      0       5     654 
   Size:          5     649
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     456     654 
   Size:        456     198
 
HaeII r_GCGC'y

Cuts at:      0     216     260     312     654 
   Size:        216      44      52     342

  Fragments arranged by size: 

                 342     216      52      44
 
KasI G'GCGC_C

Cuts at:      0     256     654 
   Size:        256     398
 
MscI TGG'CCA

Cuts at:      0     206     654 
   Size:        206     448
 
MslI CAynn'nnrTG

Cuts at:      0     133     160     361     595     654 
   Size:        133      27     201     234      59

  Fragments arranged by size: 

                 234     201     133      59      27
 
MspA1I CmG'CkG

Cuts at:      0     202     654 
   Size:        202     452
 
NarI GG'CG_CC

Cuts at:      0     257     654 
   Size:        257     397
 
PpuMI rG'GwC_Cy

Cuts at:      0       5     654 
   Size:          5     649
 
PstI C_TGCA'G

Cuts at:      0     638     654 
   Size:        638      16
 
SanDI GG'GwC_CC

Cuts at:      0       5     654 
   Size:          5     649
 
SfcI C'TryA_G

Cuts at:      0     634     654 
   Size:        634      20
 
SfoI GGC'GCC

Cuts at:      0     258     654 
   Size:        258     396
 
SmaI CCC'GGG

Cuts at:      0      10     572     654 
   Size:         10     562      82

  Fragments arranged by size: 

                 562      82      10
 
StyI C'CwwG_G

Cuts at:      0     464     654 
   Size:        464     190
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     550     582     654 
   Size:        550      32      72

  Fragments arranged by size: 

                 550      72      32
 
XmaI C'CCGG_G

Cuts at:      0       8     570     654 
   Size:          8     562      84

  Fragments arranged by size: 

                 562      84       8
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AloI    AlwNI     AvaI     BanI     BbeI
    BbvCI    BglII Bme1580I     BpmI   Bpu10I     BsaI    BsaHI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BstXI    BstYI     BtgI    BtgZI
     BtsI     EaeI  Eco57MI EcoO109I     FalI    HaeII     KasI     MscI
     MslI   MspA1I     NarI    PpuMI     PstI    SanDI     SfcI     SfoI
     SmaI     StyI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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