Restriction map for
pFN21AB0426
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BfaI BlnI BlpI Bpu1102I
BsiWI BsmBI Bsp1407I BspDI BsrDI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HgaI HindIII HpaI
KspI MaeI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SacII SalI SbfI ScaI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 1612 GGTACC G^GTACC
AccII 64 CGCG CG^CG
AccIII 1521 TCCGGA T^CCGGA
AcsI 2110 GAATTT [GA]^AATT[CT]
AcyI 11 GGCGCC G[GA]^CG[CT]C
AflII 214 CTTAAG C^TTAAG
AflIII 1406 ACATGT A^C[GA][CT]GT
AocI 1987 CCTCAGG CC^T[ACGT]AGG
Aor51HI 581 AGCGCT AGC^GCT
ApoI 2110 GAATTT [GA]^AATT[CT]
Asp718I 1612 GGTACC G^GTACC
BbeI 11 GGCGCC GGCGC^C
BbvCI 1888 GCTGAGG CCTCAGC(-5/-2)
BfrI 214 CTTAAG C^TTAAG
BglI 2480 GCCGGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2506 TACGTG [CT]AC^GT[GA]
BsaBI 1500 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 11 GGCGCC G[GA]^CG[CT]C
BseAI 1521 TCCGGA T^CCGGA
BsiEI 356 CGATCG CG[GA][CT]^CG
BsmI 1527 GAATGC GAATGC(1/-1)
BspEI 1521 TCCGGA T^CCGGA
BspHI 2159 TCATGA T^CATGA
BspLU11I 1406 ACATGT A^CATGT
BsrFI 1202 GCCGGC [GA]^CCGG[CT]
BstAPI 2107 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 280 TTCGAA TT^CGAA
BstUI 64 CGCG CG^CG
BstXI 2501 CCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1987 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1202 GCCGGC [GA]^CCGG[CT]
DraIII 61 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 581 AGCGCT AGC^GCT
Eco81I 1987 CCTCAGG CC^T[ACGT]AGG
FseI 1201 GGCCGGCC GGCCGG^CC
Hin1I 11 GGCGCC G[GA]^CG[CT]C
HincII 178 GTCAAC GT[CT]^[GA]AC
HindII 178 GTCAAC GT[CT]^[GA]AC
KasI 11 GGCGCC G^GCGCC
KpnI 1612 GGTACC GGTAC^C
MamI 1500 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1521 TCCGGA T^CCGGA
MslI 1233 CACCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 64 CGCG CG^CG
NaeI 1202 GCCGGC GCC^GGC
NarI 11 GGCGCC GG^CGCC
NgoMIV 1202 GCCGGC G^CCGGC
NspI 1406 ACATGT [GA]CATG^[CT]
NspV 280 TTCGAA TT^CGAA
PstI 849 CTGCAG CTGCA^G
PvuI 356 CGATCG CGAT^CG
RcaI 2159 TCATGA T^CATGA
SapI 276 GCTCTTC GCTCTTC(1/4)
SexAI 800 ACCAGGT A^CC[AT]GGT
SfoI 11 GGCGCC GGC^GCC
SfuI 280 TTCGAA TT^CGAA
SmaI 899 CCCGGG CCC^GGG
StuI 1589 AGGCCT AGG^CCT
XmaCI 899 CCCGGG C^CCGGG
XmaI 899 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 274 GAGCTC G[AT]GC[AT]^C
1876 GAGCTC
AvaI 899 CCCGGG C^[CT]CG[GA]G
2384 CCCGAG
BalI 1502 TGGCCA TGG^CCA
2054 TGGCCA
BbsI 415 GAAGAC GAAGAC(2/6)
805 GTCTTC
BglII 515 AGATCT A^GATCT
2471 AGATCT
BpuAI 415 GAAGAC GAAGAC(2/6)
805 GTCTTC
BsaWI 1521 TCCGGA [AT]^CCGG[AT]
2180 ACCGGA
BsiHKAI 274 GAGCTC G[AT]GC[AT]^C
1876 GAGCTC
BsoBI 899 CCCGGG C^[CT]CG[GA]G
2384 CCCGAG
BsrBI 2061 CCGCTC CCGCTC(-3/-3)
2029 GAGCGG
DsaI 1977 CCATGG C^C[GA][CT]GG
2024 CCATGG
MluNI 1502 TGGCCA TGG^CCA
2054 TGGCCA
MscI 1502 TGGCCA TGG^CCA
2054 TGGCCA
NcoI 1977 CCATGG C^CATGG
2024 CCATGG
PvuII 133 CAGCTG CAG^CTG
1159 CAGCTG
SacI 274 GAGCTC GAGCT^C
1876 GAGCTC
SfcI 423 CTACAG C^T[GA][CT]AG
849 CTGCAG
Tsp45I 67 GTGAC ^GT[GC]AC
2417 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII