(Circular) (Six-base) MAPSORT of: pFN21AB0426.seq Check: 2513 from: 1 to: 7240 pFN21AB0426 7240 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2255 2255 Size: 7240 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5050 278 Size: 53 83 186 936 3514 2468 Fragments arranged by size: 3514 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4583 1937 Size: 2646 4594 Acc65I G'GTAC_C Cuts at: 3617 4561 3617 Size: 944 6296 AclI AA'CG_TT Cuts at: 1563 5367 5740 1563 Size: 3804 373 3063 Fragments arranged by size: 3804 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3363 5303 6687 6955 1632 Size: 1731 1940 1384 268 1917 Fragments arranged by size: 1940 1917 1731 1384 268 AfeI AGC'GCT Cuts at: 2588 2588 Size: 7240 AflII C'TTAA_G Cuts at: 820 1017 2219 820 Size: 197 1202 5841 Fragments arranged by size: 5841 1202 197 AflIII A'CryG_T Cuts at: 1203 3411 6139 7116 1203 Size: 2208 2728 977 1327 Fragments arranged by size: 2728 2208 1327 977 AgeI A'CCGG_T Cuts at: 6071 6071 Size: 7240 AhdI GACnn_n'nnGTC Cuts at: 5969 5969 Size: 7240 AleI CACnn'nnGTG Cuts at: 1166 3242 1166 Size: 2076 5164 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7240 AlwNI CAG_nnn'CTG Cuts at: 2212 3031 3193 3607 4000 4983 6555 2212 Size: 819 162 414 393 983 1572 2897 Fragments arranged by size: 2897 1572 983 819 414 393 162 ApaLI G'TGCA_C Cuts at: 5297 6453 5297 Size: 1156 6084 ApoI r'AATT_y Cuts at: 1493 4115 4549 4775 4927 7036 1493 Size: 2622 434 226 152 2109 1697 Fragments arranged by size: 2622 2109 1697 434 226 152 AseI AT'TA_AT Cuts at: 160 1032 5794 160 Size: 872 4762 1606 Fragments arranged by size: 4762 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7240 AvaI C'yCGr_G Cuts at: 1870 1943 2904 4389 4565 1870 Size: 73 961 1485 176 4545 Fragments arranged by size: 4545 1485 961 176 73 BamHI G'GATC_C Cuts at: 4570 4937 4570 Size: 367 6873 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2016 2780 2986 3437 3617 Size: 325 217 627 229 764 206 451 180 Cuts at: 3617 3648 4561 6016 618 Size: 31 913 1455 1842 Fragments arranged by size: 1842 1455 913 764 627 451 325 229 217 206 180 31 BanII G_rGCy'C Cuts at: 1391 2089 2283 2550 2648 3757 3885 4559 1391 Size: 698 194 267 98 1109 128 674 4072 Fragments arranged by size: 4072 1109 698 674 267 194 128 98 BbeI G_GCGC'C Cuts at: 2020 2020 Size: 7240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2427 2803 928 Size: 971 528 376 5365 Fragments arranged by size: 5365 971 528 376 BbvCI CC'TCA_GC Cuts at: 3894 3894 Size: 7240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5431 5465 1903 Size: 34 3494 34 3678 Fragments arranged by size: 3678 3494 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5132 6342 5132 Size: 1210 6030 BclI T'GATC_A Cuts at: 1548 1548 Size: 7240 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4491 5851 136 Size: 107 122 71 631 605 2819 1360 1525 Fragments arranged by size: 2819 1525 1360 631 605 122 107 71 BglII A'GATC_T Cuts at: 2520 4476 7235 2520 Size: 1956 2759 2525 Fragments arranged by size: 2759 2525 1956 BlpI GC'TnA_GC Cuts at: 4657 4657 Size: 7240 Bme1580I G_kGCm'C Cuts at: 1790 2783 3442 5301 6457 1790 Size: 993 659 1859 1156 2573 Fragments arranged by size: 2573 1859 1156 993 659 BmgBI CAC'GTC Cuts at: 1318 2914 1318 Size: 1596 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2200 4971 5929 Size: 416 53 83 399 24 783 2771 958 Cuts at: 5929 442 Size: 1753 Fragments arranged by size: 2771 1753 958 783 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7240 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3705 3705 Size: 7240 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2948 3008 3855 3888 5900 1239 Size: 1709 60 847 33 2012 2579 Fragments arranged by size: 2579 2012 1709 847 60 33 Bpu10I CC'TnA_GC Cuts at: 2088 3756 3894 2088 Size: 1668 138 5434 Fragments arranged by size: 5434 1668 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2252 2679 3215 3317 4397 5365 6230 6528 2252 Size: 427 536 102 1080 968 865 298 2964 Fragments arranged by size: 2964 1080 968 865 536 427 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3378 3572 4386 5903 882 Size: 617 1879 194 814 1517 2219 Fragments arranged by size: 2219 1879 1517 814 617 194 BsaAI yAC'GTr Cuts at: 493 1204 4513 5031 493 Size: 711 3309 518 2702 Fragments arranged by size: 3309 2702 711 518 BsaBI GATnn'nnATC Cuts at: 3509 7234 3509 Size: 3725 3515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2017 5047 5429 6064 Size: 53 83 186 936 484 3030 382 635 Cuts at: 6064 275 Size: 1451 Fragments arranged by size: 3030 1451 936 635 484 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3526 4185 5673 6071 6345 6492 6903 3526 Size: 659 1488 398 274 147 411 3863 Fragments arranged by size: 3863 1488 659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 2612 2642 3861 Size: 30 165 30 1134 30 45 30 1219 Cuts at: 3861 3891 1178 Size: 30 4527 Fragments arranged by size: 4527 1219 1134 165 45 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 2417 2480 2516 2636 3590 3981 1186 Size: 1228 3 63 36 120 954 391 4445 Fragments arranged by size: 4445 1228 954 391 120 63 36 3 BseYI C'CCAG_C Cuts at: 2060 2641 3778 6443 6939 2060 Size: 581 1137 2665 496 2361 Fragments arranged by size: 2665 2361 1137 581 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2330 2936 3335 3645 1163 Size: 573 594 606 399 310 4758 Fragments arranged by size: 4758 606 594 573 399 310 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2364 4690 5451 6479 1540 Size: 275 186 363 2326 761 1028 2301 Fragments arranged by size: 2326 2301 1028 761 363 275 186 BsiHKAI G_wGCw'C Cuts at: 2283 3885 5301 5386 6457 2283 Size: 1602 1416 85 1071 3066 Fragments arranged by size: 3066 1602 1416 1071 85 BsmI GAATG_Cn' Cuts at: 3538 4751 4844 3538 Size: 1213 93 5934 Fragments arranged by size: 5934 1213 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2283 2550 2648 2783 3442 3757 Size: 399 299 194 267 98 135 659 315 Cuts at: 3757 3885 4559 5301 5386 6457 1391 Size: 128 674 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 674 659 399 315 299 267 194 135 128 98 85 BspEI T'CCGG_A Cuts at: 3526 6903 3526 Size: 3377 3863 BspHI T'CATG_A Cuts at: 4164 5129 6859 4164 Size: 965 1730 4545 Fragments arranged by size: 4545 1730 965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 2181 2196 2255 2739 2985 3078 3357 Size: 1316 21 15 59 484 246 93 279 Cuts at: 3357 4595 844 Size: 1238 3489 Fragments arranged by size: 3489 1316 1238 484 279 246 93 59 21 15 BsrBI CCG'CTC Cuts at: 1394 1823 4036 4068 5127 1394 Size: 429 2213 32 1059 3507 Fragments arranged by size: 3507 2213 1059 429 32 BsrDI GCAATG_nn' Cuts at: 65 1248 5735 5909 65 Size: 1183 4487 174 1396 Fragments arranged by size: 4487 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3207 5884 6071 1700 Size: 110 1397 2677 187 2869 Fragments arranged by size: 2869 2677 1397 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7240 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7240 BssSI C'ACGA_G Cuts at: 5300 6312 5300 Size: 1012 6228 BstAPI GCAn_nnn'nTGC Cuts at: 794 4118 794 Size: 3324 3916 BstBI TT'CG_AA Cuts at: 2286 6044 2286 Size: 3758 3482 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7240 BstXI CCAn_nnnn'nTGG Cuts at: 4513 4513 Size: 7240 BstYI r'GATC_y Cuts at: 1972 2520 2607 4434 4476 4570 4937 5322 5339 Size: 548 87 1827 42 94 367 385 17 Cuts at: 5339 6791 6877 6889 7235 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1827 1452 548 385 367 346 94 87 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 3993 3993 Size: 7240 BtgI C'CryG_G Cuts at: 513 1065 2004 3982 4029 513 Size: 552 939 1978 47 3724 Fragments arranged by size: 3724 1978 939 552 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3068 3731 487 Size: 683 56 1842 663 3996 Fragments arranged by size: 3996 1842 683 663 56 BtsI GCAGTG_nn' Cuts at: 3140 4341 4756 5550 5570 3140 Size: 1201 415 794 20 4810 Fragments arranged by size: 4810 1201 794 415 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7240 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4445 4480 529 Size: 35 3881 35 3289 Fragments arranged by size: 3881 3289 35 35 DraI TTT'AAA Cuts at: 4544 4544 Size: 7240 DraIII CAC_nnn'GTG Cuts at: 2071 2071 Size: 7240 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6247 809 Size: 507 4931 1802 Fragments arranged by size: 4931 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2845 2895 3205 Size: 54 1425 50 275 18 1015 50 310 Cuts at: 3205 3507 3697 4059 4483 4687 5576 7205 8 Size: 302 190 362 424 204 889 1629 43 Fragments arranged by size: 1629 1425 1015 889 424 362 310 302 275 204 190 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4687 1537 Size: 275 2875 4090 Fragments arranged by size: 4090 2875 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2288 2306 3382 5170 1353 Size: 935 18 1076 1788 3423 Fragments arranged by size: 3423 1788 1076 935 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2522 5827 6201 6347 387 Size: 1064 138 933 3305 374 146 1280 Fragments arranged by size: 3305 1280 1064 933 374 146 138 EcoICRI GAG'CTC Cuts at: 2281 3883 2281 Size: 1602 5638 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2948 3008 3363 3855 3888 5303 5900 Size: 393 1316 60 355 492 33 1415 597 Cuts at: 5900 6687 6955 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1415 1316 787 597 492 393 355 268 60 33 EcoNI CCTnn'n_nnAGG Cuts at: 2177 3023 3425 2177 Size: 846 402 5992 Fragments arranged by size: 5992 846 402 EcoO109I rG'GnC_Cy Cuts at: 2110 2476 2530 2728 2974 3097 3685 3997 4025 Size: 366 54 198 246 123 588 312 28 Cuts at: 4025 2110 Size: 5325 Fragments arranged by size: 5325 588 366 312 246 198 123 54 28 EcoRI G'AATT_C Cuts at: 4549 4927 4549 Size: 378 6862 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4279 4279 Size: 7240 FseI GG_CCGG'CC Cuts at: 3211 3211 Size: 7240 FspI TGC'GCA Cuts at: 5746 5746 Size: 7240 HaeII r_GCGC'y Cuts at: 1678 2020 2590 3256 6387 1678 Size: 342 570 666 3131 2531 Fragments arranged by size: 3131 2531 666 570 342 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 3569 3601 4461 Size: 32 135 32 965 32 1000 32 860 Cuts at: 4461 4493 5881 5913 5955 5987 1373 Size: 32 1388 32 42 32 2626 Fragments arranged by size: 2626 1388 1000 965 860 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2185 4584 4830 669 Size: 988 281 247 2399 246 3079 Fragments arranged by size: 3079 2399 988 281 247 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7240 HpaI GTT'AAC Cuts at: 4830 4830 Size: 7240 KasI G'GCGC_C Cuts at: 2016 2016 Size: 7240 KpnI G_GTAC'C Cuts at: 3621 4565 3621 Size: 944 6296 MfeI C'AATT_G Cuts at: 4839 4839 Size: 7240 MluI A'CGCG_T Cuts at: 7116 7116 Size: 7240 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2466 3540 6354 6538 1305 Size: 1161 1074 2814 184 2007 Fragments arranged by size: 2814 2007 1161 1074 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3509 4061 10 Size: 54 1425 343 1677 552 3189 Fragments arranged by size: 3189 1677 1425 552 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3242 5198 5557 5716 518 Size: 648 2076 1956 359 159 2042 Fragments arranged by size: 2076 2042 1956 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2140 3166 3639 4656 5333 6481 6726 1606 Size: 534 1026 473 1017 677 1148 245 2120 Fragments arranged by size: 2120 1148 1026 1017 677 534 473 245 NaeI GCC'GGC Cuts at: 3209 3209 Size: 7240 NarI GG'CG_CC Cuts at: 2017 2017 Size: 7240 NcoI C'CATG_G Cuts at: 513 1065 2004 3982 4029 513 Size: 552 939 1978 47 3724 Fragments arranged by size: 3724 1978 939 552 47 NdeI CA'TA_TG Cuts at: 387 387 Size: 7240 NgoMIV G'CCGG_C Cuts at: 3207 3207 Size: 7240 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7240 NotI GC'GGCC_GC Cuts at: 4687 4687 Size: 7240 NspI r_CATG'y Cuts at: 1132 1229 3415 4598 6143 1132 Size: 97 2186 1183 1545 2229 Fragments arranged by size: 2229 2186 1545 1183 97 PasI CC'CwG_GG Cuts at: 1348 2776 3442 1348 Size: 1428 666 5146 Fragments arranged by size: 5146 1428 666 PciI A'CATG_T Cuts at: 3411 6139 3411 Size: 2728 4512 PflMI CCAn_nnn'nTGG Cuts at: 1108 3031 3193 3833 1108 Size: 1923 162 640 4515 Fragments arranged by size: 4515 1923 640 162 PfoI T'CCnGG_A Cuts at: 2115 2351 3024 2115 Size: 236 673 6331 Fragments arranged by size: 6331 673 236 PmeI GTTT'AAAC Cuts at: 4544 4544 Size: 7240 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5309 5341 6822 6854 5309 Size: 32 1481 32 5695 Fragments arranged by size: 5695 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 2530 2728 2974 3097 3685 3997 2476 Size: 54 198 246 123 588 312 5719 Fragments arranged by size: 5719 588 312 246 198 123 54 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7240 PsiI TTA'TAA Cuts at: 4810 4810 Size: 7240 PstI C_TGCA'G Cuts at: 830 2858 4592 830 Size: 2028 1734 3478 Fragments arranged by size: 3478 2028 1734 PvuI CG_AT'CG Cuts at: 2001 2364 2001 Size: 363 6877 PvuII CAG'CTG Cuts at: 2140 3166 2140 Size: 1026 6214 SacI G_AGCT'C Cuts at: 2283 3885 2283 Size: 1602 5638 SalI G'TCGA_C Cuts at: 1936 4582 1936 Size: 2646 4594 SanDI GG'GwC_CC Cuts at: 3685 3685 Size: 7240 SapI GCTCTTCn'nnn_ Cuts at: 2288 2288 Size: 7240 SbfI CC_TGCA'GG Cuts at: 4592 4592 Size: 7240 ScaI AGT'ACT Cuts at: 5488 7200 5488 Size: 1712 5528 SexAI A'CCwGG_T Cuts at: 2805 2805 Size: 7240 SfcI C'TryA_G Cuts at: 826 1045 2428 2854 4588 5723 6404 6595 826 Size: 219 1383 426 1734 1135 681 191 1471 Fragments arranged by size: 1734 1471 1383 1135 681 426 219 191 SfoI GGC'GCC Cuts at: 2018 2018 Size: 7240 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7240 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7240 SmaI CCC'GGG Cuts at: 1872 2906 4567 1872 Size: 1034 1661 4545 Fragments arranged by size: 4545 1661 1034 SmlI C'TyrA_G Cuts at: 820 1017 1943 2219 2267 2694 3230 3332 4412 Size: 197 926 276 48 427 536 102 1080 Cuts at: 4412 5344 6245 6507 820 Size: 932 901 262 1553 Fragments arranged by size: 1553 1080 932 926 901 536 427 276 262 197 102 48 SnaBI TAC'GTA Cuts at: 493 5031 493 Size: 4538 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7240 SphI G_CATG'C Cuts at: 1132 4598 1132 Size: 3466 3774 SspI AAT'ATT Cuts at: 5 52 5164 5 Size: 47 5112 2081 Fragments arranged by size: 5112 2081 47 StuI AGG'CCT Cuts at: 1517 3596 1517 Size: 2079 5161 StyI C'CwwG_G Cuts at: 513 1065 2004 3433 3982 4029 4679 513 Size: 552 939 1429 549 47 650 3074 Fragments arranged by size: 3074 1429 939 650 552 549 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 3402 4410 5267 5284 5437 5622 Size: 1255 462 1360 1008 857 17 153 185 Cuts at: 5622 6067 325 Size: 445 1498 Fragments arranged by size: 1498 1360 1255 1008 857 462 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2628 5486 7198 Size: 274 80 33 51 1443 651 2858 1712 Cuts at: 7198 96 Size: 138 Fragments arranged by size: 2858 1712 1443 651 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3861 3893 7200 7232 3861 Size: 32 3307 32 3869 Fragments arranged by size: 3869 3307 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7240 XbaI T'CTAG_A Cuts at: 4576 4576 Size: 7240 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2881 3097 3178 2881 Size: 216 81 6943 Fragments arranged by size: 6943 216 81 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7240 XmaI C'CCGG_G Cuts at: 1870 2904 4565 1870 Size: 1034 1661 4545 Fragments arranged by size: 4545 1661 1034 XmnI GAAnn'nnTTC Cuts at: 5369 5369 Size: 7240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5048 276 Size: 53 83 186 936 3514 2468 Fragments arranged by size: 3514 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SrfI SwaI