(Circular) (Six-base) MAPSORT of: pFN21AE1910.seq Check: 3462 from: 1 to: 6037 pFN21AE1910 6037 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2031 2726 2031 Size: 695 5342 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3847 278 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3380 1937 Size: 1443 4594 Acc65I G'GTAC_C Cuts at: 3358 3358 Size: 6037 AclI AA'CG_TT Cuts at: 1563 4164 4537 1563 Size: 2601 373 3063 Fragments arranged by size: 3063 2601 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 2488 3107 4100 5484 5752 1632 Size: 746 110 619 993 1384 268 1917 Fragments arranged by size: 1917 1384 993 746 619 268 110 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5840 AflIII A'CryG_T Cuts at: 1203 2148 4936 5913 1203 Size: 945 2788 977 1327 Fragments arranged by size: 2788 1327 977 945 AgeI A'CCGG_T Cuts at: 4868 4868 Size: 6037 AhdI GACnn_n'nnGTC Cuts at: 4766 4766 Size: 6037 AleI CACnn'nnGTG Cuts at: 1166 2540 1166 Size: 1374 4663 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6037 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3290 3322 3290 Size: 32 6005 AlwNI CAG_nnn'CTG Cuts at: 2995 3133 3780 5352 2995 Size: 138 647 1572 3680 Fragments arranged by size: 3680 1572 647 138 ApaI G_GGCC'C Cuts at: 2854 2854 Size: 6037 ApaLI G'TGCA_C Cuts at: 2018 2570 4094 5250 2018 Size: 552 1524 1156 2805 Fragments arranged by size: 2805 1524 1156 552 ApoI r'AATT_y Cuts at: 1493 3346 3572 3724 5833 1493 Size: 1853 226 152 2109 1697 Fragments arranged by size: 2109 1853 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4591 160 Size: 872 3559 1606 Fragments arranged by size: 3559 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 AvaI C'yCGr_G Cuts at: 1870 1943 2731 2864 3362 1870 Size: 73 788 133 498 4545 Fragments arranged by size: 4545 788 498 133 73 BamHI G'GATC_C Cuts at: 3036 3367 3734 3036 Size: 331 367 5339 Fragments arranged by size: 5339 367 331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 2512 2860 3358 4813 Size: 325 217 627 429 296 348 498 1455 Cuts at: 4813 618 Size: 1842 Fragments arranged by size: 1842 1455 627 498 429 348 325 296 217 BanII G_rGCy'C Cuts at: 1391 2550 2854 2871 3356 1391 Size: 1159 304 17 485 4072 Fragments arranged by size: 4072 1159 485 304 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3059 928 Size: 971 1160 3906 Fragments arranged by size: 3906 1160 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2032 2066 3007 3041 4228 Size: 34 50 34 11 34 941 34 1187 Cuts at: 4228 4262 1903 Size: 34 3678 Fragments arranged by size: 3678 1187 941 50 34 34 34 34 34 11 BciVI GTATCCnnnnn_n' Cuts at: 3929 5139 3929 Size: 1210 4827 BclI T'GATC_A Cuts at: 1548 1548 Size: 6037 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 2397 4648 Size: 107 122 71 631 605 368 357 2251 Cuts at: 4648 136 Size: 1525 Fragments arranged by size: 2251 1525 631 605 368 357 122 107 71 BglII A'GATC_T Cuts at: 2253 6032 2253 Size: 3779 2258 BlpI GC'TnA_GC Cuts at: 2996 3454 2996 Size: 458 5579 Bme1580I G_kGCm'C Cuts at: 1790 2022 2319 2450 2517 2574 2854 2865 4098 Size: 232 297 131 67 57 280 11 1233 Cuts at: 4098 5254 1790 Size: 1156 2573 Fragments arranged by size: 2573 1233 1156 297 280 232 131 67 57 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6037 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 3768 4726 Size: 416 53 83 399 24 897 1454 958 Cuts at: 4726 442 Size: 1753 Fragments arranged by size: 1753 1454 958 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6037 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 4697 1239 Size: 917 2541 2579 Fragments arranged by size: 2579 2541 917 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 4162 5027 5325 2633 Size: 1529 865 298 3345 Fragments arranged by size: 3345 1529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 4700 882 Size: 617 1092 2109 2219 Fragments arranged by size: 2219 2109 1092 617 BsaAI yAC'GTr Cuts at: 493 1204 3828 493 Size: 711 2624 2702 Fragments arranged by size: 2702 2624 711 BsaBI GATnn'nnATC Cuts at: 6031 6031 Size: 6037 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3285 3844 4226 4861 Size: 53 83 186 936 1752 559 382 635 Cuts at: 4861 275 Size: 1451 Fragments arranged by size: 1752 1451 936 635 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2288 2364 2438 4470 4868 5142 5289 5700 2288 Size: 76 74 2032 398 274 147 411 2625 Fragments arranged by size: 2625 2032 411 398 274 147 76 74 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3290 3320 1178 Size: 30 165 30 1887 30 3895 Fragments arranged by size: 3895 1887 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 3311 3326 3329 3338 3341 1186 Size: 1303 822 15 3 9 3 3882 Fragments arranged by size: 3882 1303 822 15 9 3 3 BseYI C'CCAG_C Cuts at: 2419 2874 5240 5736 2419 Size: 455 2366 496 2720 Fragments arranged by size: 2720 2366 496 455 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 3023 1163 Size: 573 1041 246 4177 Fragments arranged by size: 4177 1041 573 246 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2264 2929 3060 3294 3487 4248 Size: 275 186 263 665 131 234 193 761 Cuts at: 4248 5276 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 665 275 263 234 193 186 131 BsiHKAI G_wGCw'C Cuts at: 2022 2574 2871 4098 4183 5254 2022 Size: 552 297 1227 85 1071 2805 Fragments arranged by size: 2805 1227 1071 552 297 85 BsmI GAATG_Cn' Cuts at: 3548 3641 3548 Size: 93 5944 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2319 2450 2517 2550 2574 2854 Size: 399 232 297 131 67 33 24 280 Cuts at: 2854 2865 2871 3356 4098 4183 5254 1391 Size: 11 6 485 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 485 399 297 280 232 131 85 67 33 24 11 6 BspEI T'CCGG_A Cuts at: 2288 2364 2438 5700 2288 Size: 76 74 3262 2625 Fragments arranged by size: 3262 2625 76 74 BspHI T'CATG_A Cuts at: 2493 3926 5656 2493 Size: 1433 1730 2874 Fragments arranged by size: 2874 1730 1433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2031 2136 2726 2760 3392 844 Size: 1187 105 590 34 632 3489 Fragments arranged by size: 3489 1187 632 590 105 34 BsrBI CCG'CTC Cuts at: 1394 1823 2192 2706 3924 1394 Size: 429 369 514 1218 3507 Fragments arranged by size: 3507 1218 514 429 369 BsrDI GCAATG_nn' Cuts at: 65 1248 4532 4706 65 Size: 1183 3284 174 1396 Fragments arranged by size: 3284 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 2294 4681 4868 1700 Size: 110 220 264 2387 187 2869 Fragments arranged by size: 2869 2387 264 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6037 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6037 BssSI C'ACGA_G Cuts at: 4097 5109 4097 Size: 1012 5025 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6037 BstBI TT'CG_AA Cuts at: 4841 4841 Size: 6037 BstEII G'GTnAC_C Cuts at: 1184 2710 1184 Size: 1526 4511 BstYI r'GATC_y Cuts at: 1972 2253 2466 3036 3367 3734 4119 4136 5588 Size: 281 213 570 331 367 385 17 1452 Cuts at: 5588 5674 5686 6032 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 570 385 367 346 331 281 213 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2113 2220 2923 2940 3079 3288 513 Size: 552 1048 107 703 17 139 209 3262 Fragments arranged by size: 3262 1048 703 552 209 139 107 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5298 Fragments arranged by size: 5298 683 56 BtsI GCAGTG_nn' Cuts at: 3553 4347 4367 3553 Size: 794 20 5223 Fragments arranged by size: 5223 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6037 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6002 DraI TTT'AAA Cuts at: 3341 3341 Size: 6037 DraIII CAC_nnn'GTG Cuts at: 2689 2689 Size: 6037 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5044 809 Size: 507 3728 1802 Fragments arranged by size: 3728 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2261 2281 Size: 54 1425 50 275 18 202 229 20 Cuts at: 2281 2694 2763 2926 2943 3057 3093 3120 3162 Size: 413 69 163 17 114 36 27 42 Cuts at: 3162 3291 3333 3484 4373 6002 8 Size: 129 42 151 889 1629 43 Fragments arranged by size: 1629 1425 889 413 275 229 202 163 151 129 114 69 54 50 43 42 42 36 27 20 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2261 2926 3057 3291 3484 1537 Size: 275 449 665 131 234 193 4090 Fragments arranged by size: 4090 665 449 275 234 193 131 EarI CTCTTCn'nnn_ Cuts at: 1353 3967 1353 Size: 2614 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3335 4624 4998 5144 387 Size: 1064 138 1746 1289 374 146 1280 Fragments arranged by size: 1746 1289 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2869 2869 Size: 6037 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2378 2488 3107 4100 4697 5484 Size: 393 524 222 110 619 993 597 787 Cuts at: 5484 5752 1239 Size: 268 1524 Fragments arranged by size: 1524 993 787 619 597 524 393 268 222 110 EcoO109I rG'GnC_Cy Cuts at: 2674 2851 2674 Size: 177 5860 EcoRI G'AATT_C Cuts at: 3346 3724 3346 Size: 378 5659 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3202 3202 Size: 6037 FseI GG_CCGG'CC Cuts at: 2034 2034 Size: 6037 FspI TGC'GCA Cuts at: 2756 4543 2756 Size: 1787 4250 HaeII r_GCGC'y Cuts at: 1678 5184 1678 Size: 3506 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3245 3277 4678 4710 4752 Size: 32 135 32 1673 32 1401 32 42 Cuts at: 4752 4784 1373 Size: 32 2626 Fragments arranged by size: 2626 1673 1401 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2770 3381 3627 669 Size: 988 281 832 611 246 3079 Fragments arranged by size: 3079 988 832 611 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6037 HpaI GTT'AAC Cuts at: 3627 3627 Size: 6037 KpnI G_GTAC'C Cuts at: 3362 3362 Size: 6037 MfeI C'AATT_G Cuts at: 3636 3636 Size: 6037 MluI A'CGCG_T Cuts at: 5913 5913 Size: 6037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5151 5335 1305 Size: 3846 184 2007 Fragments arranged by size: 3846 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2283 10 Size: 54 1425 343 451 3764 Fragments arranged by size: 3764 1425 451 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 2969 3164 3995 4354 4513 518 Size: 648 1374 429 195 831 359 159 2042 Fragments arranged by size: 2042 1374 831 648 429 359 195 159 MspA1I CmG'CkG Cuts at: 1606 2407 2578 2740 3081 3453 4130 5278 5523 Size: 801 171 162 341 372 677 1148 245 Cuts at: 5523 1606 Size: 2120 Fragments arranged by size: 2120 1148 801 677 372 341 245 171 162 NaeI GCC'GGC Cuts at: 2032 2296 2032 Size: 264 5773 NcoI C'CATG_G Cuts at: 513 1065 2113 2220 513 Size: 552 1048 107 4330 Fragments arranged by size: 4330 1048 552 107 NdeI CA'TA_TG Cuts at: 387 387 Size: 6037 NgoMIV G'CCGG_C Cuts at: 2030 2294 2030 Size: 264 5773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6037 NotI GC'GGCC_GC Cuts at: 3484 3484 Size: 6037 NspI r_CATG'y Cuts at: 1132 1229 2968 3395 4940 1132 Size: 97 1739 427 1545 2229 Fragments arranged by size: 2229 1739 1545 427 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6037 PciI A'CATG_T Cuts at: 4936 4936 Size: 6037 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 2419 2581 1108 Size: 956 355 162 4564 Fragments arranged by size: 4564 956 355 162 PfoI T'CCnGG_A Cuts at: 2352 3126 2352 Size: 774 5263 PmeI GTTT'AAAC Cuts at: 3341 3341 Size: 6037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3209 3241 3290 3322 4106 4138 5619 5651 3209 Size: 32 49 32 784 32 1481 32 3595 Fragments arranged by size: 3595 1481 784 49 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2674 2674 Size: 6037 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6037 PsiI TTA'TAA Cuts at: 3607 3607 Size: 6037 PspOMI G'GGCC_C Cuts at: 2850 2850 Size: 6037 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3005 3037 3005 Size: 32 6005 PstI C_TGCA'G Cuts at: 830 2028 3389 830 Size: 1198 1361 3478 Fragments arranged by size: 3478 1361 1198 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6037 PvuII CAG'CTG Cuts at: 2407 2578 2740 2407 Size: 171 162 5704 Fragments arranged by size: 5704 171 162 SacI G_AGCT'C Cuts at: 2871 2871 Size: 6037 SacII CC_GC'GG Cuts at: 3082 3082 Size: 6037 SalI G'TCGA_C Cuts at: 1936 3379 1936 Size: 1443 4594 SbfI CC_TGCA'GG Cuts at: 2028 3389 2028 Size: 1361 4676 ScaI AGT'ACT Cuts at: 4285 5997 4285 Size: 1712 4325 SexAI A'CCwGG_T Cuts at: 2685 3255 2685 Size: 570 5467 SfcI C'TryA_G Cuts at: 826 1045 2024 3385 4520 5201 5392 826 Size: 219 979 1361 1135 681 191 1471 Fragments arranged by size: 1471 1361 1135 979 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6037 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6037 SmaI CCC'GGG Cuts at: 1872 2733 3364 1872 Size: 861 631 4545 Fragments arranged by size: 4545 861 631 SmlI C'TyrA_G Cuts at: 820 1017 1943 2648 4141 5042 5304 820 Size: 197 926 705 1493 901 262 1553 Fragments arranged by size: 1553 1493 926 901 705 262 197 SnaBI TAC'GTA Cuts at: 493 3828 493 Size: 3335 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6037 SphI G_CATG'C Cuts at: 1132 3395 1132 Size: 2263 3774 SspI AAT'ATT Cuts at: 5 52 3961 5 Size: 47 3909 2081 Fragments arranged by size: 3909 2081 47 StuI AGG'CCT Cuts at: 1517 3182 1517 Size: 1665 4372 StyI C'CwwG_G Cuts at: 513 1065 2113 2220 3476 513 Size: 552 1048 107 1256 3074 Fragments arranged by size: 3074 1256 1048 552 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2237 2888 4064 4081 4234 4419 4864 Size: 1255 657 651 1176 17 153 185 445 Cuts at: 4864 325 Size: 1498 Fragments arranged by size: 1498 1255 1176 657 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 4283 5995 Size: 274 80 33 51 1443 174 2132 1712 Cuts at: 5995 96 Size: 138 Fragments arranged by size: 2132 1712 1443 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5997 6029 5997 Size: 32 6005 Tth111I GACn'n_nGTC Cuts at: 1316 2228 2348 2360 1316 Size: 912 120 12 4993 Fragments arranged by size: 4993 912 120 12 XbaI T'CTAG_A Cuts at: 3373 3373 Size: 6037 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2194 2899 2977 2194 Size: 705 78 5254 Fragments arranged by size: 5254 705 78 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6037 XmaI C'CCGG_G Cuts at: 1870 2731 3362 1870 Size: 861 631 4545 Fragments arranged by size: 4545 861 631 XmnI GAAnn'nnTTC Cuts at: 2501 4166 2501 Size: 1665 4372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3845 276 Size: 53 83 186 936 2311 2468 Fragments arranged by size: 2468 2311 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SanDI SapI SfiI SfoI SrfI SwaI