################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsiEI BsiWI Bsp1407I BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 752 TCCGGA T^CCGGA AcyI 1323 GGCGCC G[GA]^CG[CT]C AflIII 114 ACACGT A^C[GA][CT]GT AocI 1272 CCTGAGG CC^T[ACGT]AGG ApaI 361 GGGCCC GGGCC^C BbeI 1323 GGCGCC GGCGC^C BbrPI 115 CACGTG CAC^GTG BbsI 271 GAAGAC GAAGAC(2/6) BbvCI 189 CCTCAGC CCTCAGC(-5/-2) BcgI 962 GCACCTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1019 GCCCTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 271 GAAGAC GAAGAC(2/6) BsaAI 115 CACGTG [CT]AC^GT[GA] BsaHI 1323 GGCGCC G[GA]^CG[CT]C BseAI 752 TCCGGA T^CCGGA BsmBI 1373 GAGACG CGTCTC(1/5) BsmI 1278 GAATGC GAATGC(1/-1) BspDI 802 ATCGAT AT^CGAT BspEI 752 TCCGGA T^CCGGA BstXI 446 CCATAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1272 CCTGAGG CC^T[ACGT]AGG ClaI 802 ATCGAT AT^CGAT DsaI 47 CCATGG C^C[GA][CT]GG EarI 1177 GAAGAG CTCTTC(1/4) Eco81I 1272 CCTGAGG CC^T[ACGT]AGG EcoT14I 47 CCATGG C^C[AT][AT]GG HaeII 1323 GGCGCC [GA]GCGC^[CT] Hin1I 1323 GGCGCC G[GA]^CG[CT]C KasI 1323 GGCGCC G^GCGCC Ksp632I 1177 GAAGAG CTCTTC(1/4) MroI 752 TCCGGA T^CCGGA NarI 1323 GGCGCC GG^CGCC NcoI 47 CCATGG C^CATGG NlaIII 48 CATG CATG^ PaeR7I 874 CTCGAG C^TCGAG PmaCI 115 CACGTG CAC^GTG PmlI 115 CACGTG CAC^GTG PvuII 697 CAGCTG CAG^CTG SacI 70 GAGCTC GAGCT^C SfoI 1323 GGCGCC GGC^GCC SmaI 1195 CCCGGG CCC^GGG SmlI 874 CTCGAG C^T[CT][GA]AG StyI 47 CCATGG C^C[AT][AT]GG TfiI 843 GAATC G^A[AT]TC Tsp509I 1237 AATT ^AATT XhoI 874 CTCGAG C^TCGAG XmaCI 1195 CCCGGG C^CCGGG XmaI 1195 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 1108 CGCG CG^CG 1224 CGCG AlwNI 157 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG 631 CAGGAGCTG BanI 961 GGCACC G^G[CT][GA]CC 1323 GGCGCC BcnI 1195 CCCGG CC^[GC]GG 1196 CCGGG BsaWI 504 TCCGGT [AT]^CCGG[AT] 752 TCCGGA BsgI 931 GTGCAG GTGCAG(16/14) 1352 GTGCAG BspMI 572 ACCTGC ACCTGC(4/8) 156 GCAGGT BstAPI 156 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 386 GCAACTCTTGC BstUI 1108 CGCG CG^CG 1224 CGCG BstYI 78 GGATCT [GA]^GATC[CT] 479 GGATCT DrdI 151 GACCAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 816 GACTCGGATGTC MflI 78 GGATCT [GA]^GATC[CT] 479 GGATCT MslI 924 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1090 CACAACGATG MspA1I 309 CAGCGG C[AC]G^C[GT]G 697 CAGCTG MvnI 1108 CGCG CG^CG 1224 CGCG NaeI 331 GCCGGC GCC^GGC 495 GCCGGC NciI 1195 CCCGG CC^[GC]GG 1196 CCGGG NgoMIV 331 GCCGGC G^CCGGC 495 GCCGGC Tsp45I 140 GTCAC ^GT[GC]AC 442 GTGAC XhoII 78 GGATCT [GA]^GATC[CT] 479 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I