Restriction map for
pFN21AE3688
(Circular) (Six-base) MAPSORT of: pFN21AE3688.seq Check: 4420 from: 1 to: 6088
pFN21AE3688 6088 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2457 2933 4151 5535 5803 1632
Size: 825 476 1218 1384 268 1917
Fragments arranged by size:
1917 1384 1218 825 476 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 2119 4987 5964 1203
Size: 916 2868 977 1327
Fragments arranged by size:
2868 1327 977 916
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2708 3117 1184
Size: 1524 409 4155
Fragments arranged by size:
4155 1524 409
AlwNI CAG_nnn'CTG
Cuts at: 2167 2641 3831 5403 2167
Size: 474 1190 1572 2852
Fragments arranged by size:
2852 1572 1190 474
ApaI G_GGCC'C
Cuts at: 2370 2370
Size: 6088
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 3397 3623 3775 5884 1493
Size: 1904 226 152 2109 1697
Fragments arranged by size:
2109 1904 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2879 3161 3177 3200 3413 1870
Size: 73 936 282 16 23 213 4545
Fragments arranged by size:
4545 936 282 213 73 23 16
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2966 3328 3409 4864 618
Size: 325 217 627 1179 362 81 1455 1842
Fragments arranged by size:
1842 1455 1179 627 362 325 217 81
BanII G_rGCy'C
Cuts at: 1391 2079 2213 2370 2474 3053 3407 1391
Size: 688 134 157 104 579 354 4072
Fragments arranged by size:
4072 688 579 354 157 134 104
BbeI G_GCGC'C
Cuts at: 3332 3332
Size: 6088
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2283 928
Size: 971 384 4733
Fragments arranged by size:
4733 971 384
BbvCI CC'TCA_GC
Cuts at: 2195 2195
Size: 6088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2956 2990 4279 4313 1903
Size: 34 1019 34 1289 34 3678
Fragments arranged by size:
3678 1289 1019 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3030 4699 136
Size: 107 122 71 631 605 1358 1669 1525
Fragments arranged by size:
1669 1525 1358 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 2370 2631 4149 5305 1790
Size: 580 261 1518 1156 2573
Fragments arranged by size:
2573 1518 1156 580 261
BmgBI CAC'GTC
Cuts at: 1318 2149 3356 1318
Size: 831 1207 4050
Fragments arranged by size:
4050 1207 831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3220 3331 3819
Size: 416 53 83 399 24 1803 111 488
Cuts at: 3819 4777 442
Size: 958 1753
Fragments arranged by size:
1803 1753 958 488 416 399 111 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2235 2837 2906 3067 3174 3386 4748 1239
Size: 996 602 69 161 107 212 1362 2579
Fragments arranged by size:
2579 1362 996 602 212 161 107 69
Bpu10I CC'TnA_GC
Cuts at: 2195 3349 2195
Size: 1154 4934
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 3879 493
Size: 711 918 1757 2702
Fragments arranged by size:
2702 1757 918 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3329 3895 4277 4912
Size: 53 83 186 936 1796 566 382 635
Cuts at: 4912 275
Size: 1451
Fragments arranged by size:
1796 1451 936 635 566 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2509 2757 4521 4919 5193 5340 5751 2509
Size: 248 1764 398 274 147 411 2846
Fragments arranged by size:
2846 1764 411 398 274 248 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 2384 2414 1178
Size: 30 165 30 867 30 84 30 4852
Fragments arranged by size:
4852 867 165 84 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 2738 3266 1186
Size: 1357 195 528 4008
Fragments arranged by size:
4008 1357 528 195
BseYI C'CCAG_C
Cuts at: 2128 2139 2228 2352 2624 3298 5291 5787 2128
Size: 11 89 124 272 674 1993 496 2429
Fragments arranged by size:
2429 1993 674 496 272 124 89 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2957 3378 1163
Size: 573 1221 421 3873
Fragments arranged by size:
3873 1221 573 421
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2649 3344 4149 4234 5305 2079
Size: 570 695 805 85 1071 2862
Fragments arranged by size:
2862 1071 805 695 570 85
BsmI GAATG_Cn'
Cuts at: 3289 3599 3692 3289
Size: 310 93 5685
Fragments arranged by size:
5685 310 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3372 3372
Size: 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2213 2370 2474 2631 2649 3053
Size: 399 289 134 157 104 157 18 404
Cuts at: 3053 3344 3407 4149 4234 5305 1391
Size: 291 63 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 404 399 291 289 157
157 134 104 85 63 18
BspEI T'CCGG_A
Cuts at: 2757 5751 2757
Size: 2994 3094
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 2586 3443 844
Size: 1308 434 857 3489
Fragments arranged by size:
3489 1308 857 434
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 2336 2500 4732 4919 1700
Size: 110 287 239 164 2232 187 2869
Fragments arranged by size:
2869 2232 287 239 187 164 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 2397 794
Size: 1373 230 4485
Fragments arranged by size:
4485 1373 230
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2458
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2083 2484 3418 3785 4170 4187 5639 5725
Size: 111 401 934 367 385 17 1452 86
Cuts at: 5725 5737 6083 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 934 401 385 367 346 111
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3278 3278
Size: 6088
BtgI C'CryG_G
Cuts at: 513 1065 2052 513
Size: 552 987 4549
Fragments arranged by size:
4549 987 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 2222 2263 2575 2838 3604 4398 4418 2222
Size: 41 312 263 766 794 20 3892
Fragments arranged by size:
3892 794 766 312 263 41 20
ClaI AT'CG_AT
Cuts at: 1554 2808 1554
Size: 1254 4834
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2162 2827 5095 809
Size: 507 846 665 2268 1802
Fragments arranged by size:
2268 1802 846 665 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053
Size: 54 1425 50 275 18 1705 889 1629
Cuts at: 6053 8
Size: 43
Fragments arranged by size:
1705 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3177 4018 1353
Size: 1824 841 3423
Fragments arranged by size:
3423 1824 841
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2735 4675 5049 5195 387
Size: 1064 138 1146 1940 374 146 1280
Fragments arranged by size:
1940 1280 1146 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2077 2077
Size: 6088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2457 2837 2906 2933 3067 3174
Size: 393 603 222 380 69 27 134 107
Cuts at: 3174 3386 4151 4748 5535 5803 1239
Size: 212 765 597 787 268 1524
Fragments arranged by size:
1524 787 765 603 597 393 380 268
222 212 134 107 69 27
EcoO109I rG'GnC_Cy
Cuts at: 2164 2366 2367 2575 2598 3145 3152 2164
Size: 202 1 208 23 547 7 5100
Fragments arranged by size:
5100 547 208 202 23 7 1
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 3332 5235 1678
Size: 1654 1903 2531
Fragments arranged by size:
2531 1903 1654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2469 2501 2520
Size: 32 135 32 698 32 167 32 19
Cuts at: 2520 2552 3092 3124 4729 4761 4803 4835 1373
Size: 32 540 32 1605 32 42 32 2626
Fragments arranged by size:
2626 1605 698 540 167 135 42 32
32 32 32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KasI G'GCGC_C
Cuts at: 3328 3328
Size: 6088
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 5202 5386 1305
Size: 1008 2889 184 2007
Fragments arranged by size:
2889 2007 1008 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4266
Fragments arranged by size:
4266 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2933 3099 4046 4405 4564 518
Size: 648 1767 166 947 359 159 2042
Fragments arranged by size:
2042 1767 947 648 359 166 159
MspA1I CmG'CkG
Cuts at: 1606 2316 2704 3504 4181 5329 5574 1606
Size: 710 388 800 677 1148 245 2120
Fragments arranged by size:
2120 1148 800 710 677 388 245
NaeI GCC'GGC
Cuts at: 2338 2502 2338
Size: 164 5924
NarI GG'CG_CC
Cuts at: 3329 3329
Size: 6088
NcoI C'CATG_G
Cuts at: 513 1065 2052 513
Size: 552 987 4549
Fragments arranged by size:
4549 987 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 2336 2500 2336
Size: 164 5924
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 3446 4991 1132
Size: 97 2217 1545 2229
Fragments arranged by size:
2229 2217 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2414 3053 1348
Size: 1066 639 4383
Fragments arranged by size:
4383 1066 639
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6088
PfoI T'CCnGG_A
Cuts at: 2079 3190 3219 2079
Size: 1111 29 4948
Fragments arranged by size:
4948 1111 29
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PmlI CAC'GTG
Cuts at: 2122 2122
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2575 3152 2164
Size: 411 577 5100
Fragments arranged by size:
5100 577 411
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 2366 2366
Size: 6088
PstI C_TGCA'G
Cuts at: 830 3440 830
Size: 2610 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 2704 2704
Size: 6088
SacI G_AGCT'C
Cuts at: 2079 2079
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 3436 4571 5252 5443 826
Size: 219 2391 1135 681 191 1471
Fragments arranged by size:
2391 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3330 3330
Size: 6088
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3202 3415 1872
Size: 1330 213 4545
Fragments arranged by size:
4545 1330 213
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2879 4192 5093 5355 820
Size: 197 926 936 1313 901 262 1553
Fragments arranged by size:
1553 1313 936 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2052 3527 513
Size: 552 987 1475 3074
Fragments arranged by size:
3074 1475 987 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3313 4115 4132 4285 4470 4915 325
Size: 1255 1733 802 17 153 185 445 1498
Fragments arranged by size:
1733 1498 1255 802 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4334 6046 96
Size: 274 80 33 51 1443 2357 1712 138
Fragments arranged by size:
2357 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6048 6080 6048
Size: 32 6056
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 2879 1943
Size: 936 5152
XmaI C'CCGG_G
Cuts at: 1870 3200 3413 1870
Size: 1330 213 4545
Fragments arranged by size:
4545 1330 213
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI