################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## Acc65I AclI AcsI AflII AflIII AgeI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MseI MunI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfuI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 820 GACGTC GACGT^C AccIII 1036 TCCGGA T^CCGGA AfaI 852 GTAC GT^AC AhdI 233 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 233 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 952 GTGCTC G[AT]GC[AT]^C AvaII 14 GGACC G^G[AT]CC BamHI 872 GGATCC G^GATCC BanI 339 GGCGCC G^G[CT][GA]CC BbeI 339 GGCGCC GGCGC^C BbsI 750 GAAGAC GAAGAC(2/6) BpuAI 750 GAAGAC GAAGAC(2/6) BsaWI 1036 TCCGGA [AT]^CCGG[AT] BseAI 1036 TCCGGA T^CCGGA BsiHKAI 952 GTGCTC G[AT]GC[AT]^C BsmAI 977 GTCTC GTCTC(1/5) BspEI 1036 TCCGGA T^CCGGA BstYI 872 GGATCC [GA]^GATC[CT] Eam1105I 233 GACGCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 904 GTCAAC GT[CT]^[GA]AC HindII 904 GTCAAC GT[CT]^[GA]AC HinfI 31 GAGTC G^A[ACGT]TC KasI 339 GGCGCC G^GCGCC MaeIII 272 GTGAC ^GT[ACGT]AC MflI 872 GGATCC [GA]^GATC[CT] MroI 1036 TCCGGA T^CCGGA NarI 339 GGCGCC GG^CGCC NcoI 437 CCATGG C^CATGG NruI 229 TCGCGA TCG^CGA PleI 31 GAGTC GAGTC(4/5) PpuMI 13 GGGACCC [GA]G^G[AT]CC[CT] PstI 416 CTGCAG CTGCA^G RsaI 852 GTAC GT^AC SfcI 416 CTGCAG C^T[GA][CT]AG SfiI 291 GGCCTACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 339 GGCGCC GGC^GCC SgrAI 636 CGCCGGCG C[GA]^CCGG[CT]G SphI 989 GCATGC GCATG^C TaqI 1025 TCGA T^CGA Tsp45I 272 GTGAC ^GT[GC]AC TthHB8I 1025 TCGA T^CGA XhoII 872 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 241 GTCTAC GT^[AC][GT]AC 484 GTCTAC Aor51HI 425 AGCGCT AGC^GCT 605 AGCGCT BalI 299 TGGCCA TGG^CCA 647 TGGCCA BglI 292 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1021 GCCGTCGAGGC BlpI 954 GCTCAGC GC^T[ACGT]AGC 1098 GCTCAGC Bpu1102I 954 GCTCAGC GC^T[ACGT]AGC 1098 GCTCAGC BspMI 275 ACCTGC ACCTGC(4/8) 854 ACCTGC BsrBI 1092 CCGCTC CCGCTC(-3/-3) 459 GAGCGG BsrI 869 ACTGG ACTGG(1/-1) 348 CCAGT BstXI 167 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 650 CCACCATCATGG CelII 954 GCTCAGC GC^T[ACGT]AGC 1098 GCTCAGC DsaI 8 CCACGG C^C[GA][CT]GG 437 CCATGG EagI 50 CGGCCG C^GGCCG 445 CGGCCG EclXI 50 CGGCCG C^GGCCG 445 CGGCCG Eco47III 425 AGCGCT AGC^GCT 605 AGCGCT Eco52I 50 CGGCCG C^GGCCG 445 CGGCCG EcoNI 357 CCTACGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 684 CCTGGTGCAGG EcoT14I 437 CCATGG C^C[AT][AT]GG 1069 CCAAGG MaeII 305 ACGT A^CGT 821 ACGT MluNI 299 TGGCCA TGG^CCA 647 TGGCCA MscI 299 TGGCCA TGG^CCA 647 TGGCCA MslI 435 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 651 CACCATCATG NspI 221 ACATGC [GA]CATG^[CT] 989 GCATGC PflMI 39 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 833 CCAACCTCTGG PvuII 43 CAGCTG CAG^CTG 168 CAGCTG SapI 372 GCTCTTC GCTCTTC(1/4) 408 GCTCTTC SmaI 1000 CCCGGG CCC^GGG 1016 CCCGGG StyI 437 CCATGG C^C[AT][AT]GG 1069 CCAAGG TspRI 349 CAGTG CA[GC]TG[ACGT][ACGT]^ 414 CACTG Van91I 39 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 833 CCAACCTCTGG XcmI 167 CCAGCTGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 674 CCATCTTCCGCCTGG XmaCI 1000 CCCGGG C^CCGGG 1016 CCCGGG XmaI 1000 CCCGGG C^CCGGG 1016 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AcyI AluI AlwI ApaI AvaI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI