(Circular) (Six-base) MAPSORT of: pFN21AE9503.seq Check: 9302 from: 1 to: 5857 pFN21AE9503 5857 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2829 3667 278 Size: 53 83 186 936 1293 838 2468 Fragments arranged by size: 2468 1293 936 838 186 83 53 AccI GT'mk_AC Cuts at: 1937 2247 2490 3200 1937 Size: 310 243 710 4594 Fragments arranged by size: 4594 710 310 243 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2389 2774 3920 5304 5572 1632 Size: 757 385 1146 1384 268 1917 Fragments arranged by size: 1917 1384 1146 757 385 268 AfeI AGC'GCT Cuts at: 2432 2612 2432 Size: 180 5677 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 2243 4586 2243 Size: 2343 3514 AleI CACnn'nnGTG Cuts at: 1166 2444 1166 Size: 1278 4579 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2972 1184 Size: 1788 4069 AlwNI CAG_nnn'CTG Cuts at: 3600 5172 3600 Size: 1572 4285 ApaI G_GGCC'C Cuts at: 2149 2329 2801 2851 3006 2149 Size: 180 472 50 155 5000 Fragments arranged by size: 5000 472 180 155 50 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 3166 3392 3544 5653 1493 Size: 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2098 3005 3021 3182 1870 Size: 73 90 65 907 16 161 4545 Fragments arranged by size: 4545 907 161 90 73 65 16 BamHI G'GATC_C Cuts at: 2877 3187 3554 2877 Size: 310 367 5180 Fragments arranged by size: 5180 367 310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2344 3178 4633 618 Size: 325 217 627 557 834 1455 1842 Fragments arranged by size: 1842 1455 834 627 557 325 217 BanII G_rGCy'C Cuts at: 1391 2149 2329 2801 2851 3006 3126 3176 1391 Size: 758 180 472 50 155 120 50 4072 Fragments arranged by size: 4072 758 472 180 155 120 50 50 BbeI G_GCGC'C Cuts at: 2348 2348 Size: 5857 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2762 928 Size: 971 863 4023 Fragments arranged by size: 4023 971 863 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2303 3032 4468 Size: 107 122 71 631 605 631 729 1436 Cuts at: 4468 136 Size: 1525 Fragments arranged by size: 1525 1436 729 631 631 605 122 107 71 BglII A'GATC_T Cuts at: 5852 5852 Size: 5857 BlpI GC'TnA_GC Cuts at: 2960 3104 3274 2960 Size: 144 170 5543 Fragments arranged by size: 5543 170 144 Bme1580I G_kGCm'C Cuts at: 1790 2067 2149 2329 2801 2851 3006 3918 5074 Size: 277 82 180 472 50 155 912 1156 Cuts at: 5074 1790 Size: 2573 Fragments arranged by size: 2573 1156 912 472 277 180 155 82 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2347 3588 4546 Size: 416 53 83 399 24 930 1241 958 Cuts at: 4546 442 Size: 1753 Fragments arranged by size: 1753 1241 958 930 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2975 3055 2975 Size: 80 5777 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2569 3124 4517 1239 Size: 971 359 555 1393 2579 Fragments arranged by size: 2579 1393 971 555 359 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3648 493 Size: 711 2444 2702 Fragments arranged by size: 2702 2444 711 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2345 2646 2826 3664 Size: 53 83 186 936 812 301 180 838 Cuts at: 3664 4046 4681 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 838 812 635 382 301 186 180 83 53 BsaWI w'CCGG_w Cuts at: 3041 4290 4688 4962 5109 5520 3041 Size: 1249 398 274 147 411 3378 Fragments arranged by size: 3378 1249 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2567 2597 3113 3143 1178 Size: 30 165 30 1164 30 516 30 3892 Fragments arranged by size: 3892 1164 516 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5857 BseYI C'CCAG_C Cuts at: 2241 2530 2548 2722 2793 2989 5060 5556 2241 Size: 289 18 174 71 196 2071 496 2542 Fragments arranged by size: 2542 2071 496 289 196 174 71 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 2714 2968 1163 Size: 573 333 645 254 4052 Fragments arranged by size: 4052 645 573 333 254 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2058 2234 2453 3307 4068 5096 Size: 275 186 57 176 219 854 761 1028 Cuts at: 5096 1540 Size: 2301 Fragments arranged by size: 2301 1028 854 761 275 219 186 176 57 BsiHKAI G_wGCw'C Cuts at: 2961 3918 4003 5074 2961 Size: 957 85 1071 3744 Fragments arranged by size: 3744 1071 957 85 BsmI GAATG_Cn' Cuts at: 3368 3461 3368 Size: 93 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2149 2329 2801 2851 2961 3006 Size: 399 277 82 180 472 50 110 45 Cuts at: 3006 3126 3176 3918 4003 5074 1391 Size: 120 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 472 399 277 180 120 110 85 82 50 50 45 BspEI T'CCGG_A Cuts at: 3041 5520 3041 Size: 2479 3378 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2289 2868 3212 844 Size: 1445 579 344 3489 Fragments arranged by size: 3489 1445 579 344 BsrBI CCG'CTC Cuts at: 1394 1823 2466 3099 3744 1394 Size: 429 643 633 645 3507 Fragments arranged by size: 3507 645 643 633 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4352 4526 65 Size: 1183 3104 174 1396 Fragments arranged by size: 3104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2057 2070 2103 2452 2642 4501 4688 Size: 110 247 13 33 349 190 1859 187 Cuts at: 4688 1700 Size: 2869 Fragments arranged by size: 2869 1859 349 247 190 187 110 33 13 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5857 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5857 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2662 2179 Size: 483 5374 BstYI r'GATC_y Cuts at: 1972 2877 3187 3554 3939 3956 5408 5494 5506 Size: 905 310 367 385 17 1452 86 12 Cuts at: 5506 5852 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 905 385 367 346 310 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2013 2442 513 Size: 552 948 429 3928 Fragments arranged by size: 3928 948 552 429 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 2418 3373 4167 4187 2418 Size: 955 794 20 4088 Fragments arranged by size: 4088 955 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2109 2166 Size: 54 1425 50 275 18 225 54 57 Cuts at: 2166 2211 2304 2391 2450 2457 2520 2652 2820 Size: 45 93 87 59 7 63 132 168 Cuts at: 2820 2889 3304 4193 5822 8 Size: 69 415 889 1629 43 Fragments arranged by size: 1629 1425 889 415 275 225 168 132 93 87 69 63 59 57 54 54 50 45 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2055 2450 3304 1537 Size: 275 243 395 854 4090 Fragments arranged by size: 4090 854 395 275 243 EarI CTCTTCn'nnn_ Cuts at: 1353 2384 2420 2627 2810 3787 1353 Size: 1031 36 207 183 977 3423 Fragments arranged by size: 3423 1031 977 207 183 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2675 4444 4818 4964 387 Size: 1064 138 1086 1769 374 146 1280 Fragments arranged by size: 1769 1280 1086 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2389 2569 2774 3124 3920 4517 Size: 393 578 179 180 205 350 796 597 Cuts at: 4517 5304 5572 1239 Size: 787 268 1524 Fragments arranged by size: 1524 796 787 597 578 393 350 268 205 180 179 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2693 2366 Size: 327 5530 EcoO109I rG'GnC_Cy Cuts at: 2019 2145 2146 2325 2371 2713 2797 3002 2019 Size: 126 1 179 46 342 84 205 4874 Fragments arranged by size: 4874 342 205 179 126 84 46 1 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 2348 2434 2614 5004 1678 Size: 670 86 180 2390 2531 Fragments arranged by size: 2531 2390 670 180 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2229 2261 2567 2599 3113 Size: 32 135 32 657 32 306 32 514 Cuts at: 3113 3145 4498 4530 4572 4604 1373 Size: 32 1353 32 42 32 2626 Fragments arranged by size: 2626 1353 657 514 306 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2911 3201 3447 669 Size: 988 281 973 290 246 3079 Fragments arranged by size: 3079 988 973 290 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KasI G'GCGC_C Cuts at: 2344 2344 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2862 4971 5155 1305 Size: 1557 2109 184 2007 Fragments arranged by size: 2109 2007 1557 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2306 2654 10 Size: 54 1425 343 474 348 3213 Fragments arranged by size: 3213 1425 474 348 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 2660 3815 4174 4333 518 Size: 648 1278 216 1155 359 159 2042 Fragments arranged by size: 2042 1278 1155 648 359 216 159 MspA1I CmG'CkG Cuts at: 1606 2050 2175 2580 2703 2726 3273 3950 5098 Size: 444 125 405 123 23 547 677 1148 Cuts at: 5098 5343 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 547 444 405 245 125 123 23 NaeI GCC'GGC Cuts at: 2059 2072 2105 2644 2059 Size: 13 33 539 5272 Fragments arranged by size: 5272 539 33 13 NarI GG'CG_CC Cuts at: 2345 2345 Size: 5857 NcoI C'CATG_G Cuts at: 513 1065 2442 513 Size: 552 1377 3928 Fragments arranged by size: 3928 1377 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NgoMIV G'CCGG_C Cuts at: 2057 2070 2103 2642 2057 Size: 13 33 539 5272 Fragments arranged by size: 5272 539 33 13 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NruI TCG'CGA Cuts at: 2236 2236 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 2230 2998 3215 4760 1132 Size: 97 1001 768 217 1545 2229 Fragments arranged by size: 2229 1545 1001 768 217 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5857 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 2050 2844 1108 Size: 942 794 4121 Fragments arranged by size: 4121 942 794 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2019 2019 Size: 5857 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PspOMI G'GGCC_C Cuts at: 2145 2325 2797 2847 3002 2145 Size: 180 472 50 155 5000 Fragments arranged by size: 5000 472 180 155 50 PstI C_TGCA'G Cuts at: 830 2425 3209 830 Size: 1595 784 3478 Fragments arranged by size: 3478 1595 784 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 2050 2175 2050 Size: 125 5732 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SanDI GG'GwC_CC Cuts at: 2019 2019 Size: 5857 SapI GCTCTTCn'nnn_ Cuts at: 2384 2420 2384 Size: 36 5821 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SfcI C'TryA_G Cuts at: 826 1045 2421 3205 4340 5021 5212 826 Size: 219 1376 784 1135 681 191 1471 Fragments arranged by size: 1471 1376 1135 784 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2303 2303 Size: 5857 SfoI GGC'GCC Cuts at: 2346 2346 Size: 5857 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 2642 1700 Size: 942 4915 SmaI CCC'GGG Cuts at: 1872 3007 3023 3184 1872 Size: 1135 16 161 4545 Fragments arranged by size: 4545 1135 161 16 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4862 5124 820 Size: 197 926 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 2998 3215 1132 Size: 1866 217 3774 Fragments arranged by size: 3774 1866 217 SrfI GCCC'GGGC Cuts at: 3023 3023 Size: 5857 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5857 StyI C'CwwG_G Cuts at: 513 1065 2442 3074 3296 513 Size: 552 1377 632 222 3074 Fragments arranged by size: 3074 1377 632 552 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3884 3901 4054 4239 4684 325 Size: 1255 2304 17 153 185 445 1498 Fragments arranged by size: 2304 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4103 5815 96 Size: 274 80 33 51 1443 2126 1712 138 Fragments arranged by size: 2126 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5817 5849 5817 Size: 32 5825 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2686 2179 Size: 507 5350 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 3005 3021 3182 1870 Size: 1135 16 161 4545 Fragments arranged by size: 4545 1135 161 16 XmnI GAAnn'nnTTC Cuts at: 3986 3986 Size: 5857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2827 3665 276 Size: 53 83 186 936 1293 838 2468 Fragments arranged by size: 2468 1293 936 838 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI SwaI