Restriction map for pFN21AE9503
 (Linear) (Six-base) MAPSORT of: KIEE9503.seq  Check: 680  from: 1  to: 1152



KIEE9503.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:40  ..

AatII G_ACGT'C

Cuts at:      0     824    1152 
   Size:        824     328
 
AccI GT'mk_AC

Cuts at:      0     242     485    1152 
   Size:        242     243     667

  Fragments arranged by size: 

                 667     243     242
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     384     769    1152 
   Size:        384     385     383

  Fragments arranged by size: 

                 385     384     383
 
AfeI AGC'GCT

Cuts at:      0     427     607    1152 
   Size:        427     180     545

  Fragments arranged by size: 

                 545     427     180
 
AhdI GACnn_n'nnGTC

Cuts at:      0     238    1152 
   Size:        238     914
 
AleI CACnn'nnGTG

Cuts at:      0     439    1152 
   Size:        439     713
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     967    1152 
   Size:        967     185
 
ApaI G_GGCC'C

Cuts at:      0     144     324     796     846    1001    1152 
   Size:        144     180     472      50     155     151

  Fragments arranged by size: 

                 472     180     155     151     144      50
 
AvaI C'yCGr_G

Cuts at:      0      28      93    1000    1016    1152 
   Size:         28      65     907      16     136

  Fragments arranged by size: 

                 907     136      65      28      16
 
BamHI G'GATC_C

Cuts at:      0     872    1152 
   Size:        872     280
 
BanI G'GyrC_C

Cuts at:      0     339    1152 
   Size:        339     813
 
BanII G_rGCy'C

Cuts at:      0     144     324     796     846    1001    1121    1152 
   Size:        144     180     472      50     155     120      31

  Fragments arranged by size: 

                 472     180     155     144     120      50      31
 
BbeI G_GCGC'C

Cuts at:      0     343    1152 
   Size:        343     809
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     757    1152 
   Size:        757     395
 
BglI GCCn_nnn'nGGC

Cuts at:      0     298    1027    1152 
   Size:        298     729     125

  Fragments arranged by size: 

                 729     298     125
 
BlpI GC'TnA_GC

Cuts at:      0     955    1099    1152 
   Size:        955     144      53

  Fragments arranged by size: 

                 955     144      53
 
Bme1580I G_kGCm'C

Cuts at:      0      62     144     324     796     846    1001    1152 
   Size:         62      82     180     472      50     155     151

  Fragments arranged by size: 

                 472     180     155     151      82      62      50
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     342    1152 
   Size:        342     810
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     970    1050    1152 
   Size:        970      80     102

  Fragments arranged by size: 

                 970     102      80
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     564    1119    1152 
   Size:        205     359     555      33

  Fragments arranged by size: 

                 555     359     205      33
 
BsaHI Gr'CG_yC

Cuts at:      0     340     641     821    1152 
   Size:        340     301     180     331

  Fragments arranged by size: 

                 340     331     301     180
 
BsaWI w'CCGG_w

Cuts at:      0    1036    1152 
   Size:       1036     116
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     562     592    1108    1138    1152 
   Size:        562      30     516      30      14

  Fragments arranged by size: 

                 562     516      30      30      14
 
BseYI C'CCAG_C

Cuts at:      0     236     525     543     717     788     984    1152 
   Size:        236     289      18     174      71     196     168

  Fragments arranged by size: 

                 289     236     196     174     168      71      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     709     963    1152 
   Size:         64     645     254     189

  Fragments arranged by size: 

                 645     254     189      64
 
BsiEI CG_ry'CG

Cuts at:      0      53     229     448    1152 
   Size:         53     176     219     704

  Fragments arranged by size: 

                 704     219     176      53
 
BsiHKAI G_wGCw'C

Cuts at:      0     956    1152 
   Size:        956     196
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     144     324     796     846     956    1001    1121 
   Size:         62      82     180     472      50     110      45     120

Cuts at:   1121    1152 
   Size:         31

  Fragments arranged by size: 

                 472     180     120     110      82      62      50      45
                  31
 
BspEI T'CCGG_A

Cuts at:      0    1036    1152 
   Size:       1036     116
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     284     863    1152 
   Size:        284     579     289

  Fragments arranged by size: 

                 579     289     284
 
BsrBI CCG'CTC

Cuts at:      0     461    1094    1152 
   Size:        461     633      58

  Fragments arranged by size: 

                 633     461      58
 
BsrFI r'CCGG_y

Cuts at:      0      52      65      98     447     637    1152 
   Size:         52      13      33     349     190     515

  Fragments arranged by size: 

                 515     349     190      52      33      13
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     174     657    1152 
   Size:        174     483     495

  Fragments arranged by size: 

                 495     483     174
 
BstYI r'GATC_y

Cuts at:      0     872    1152 
   Size:        872     280
 
BtgI C'CryG_G

Cuts at:      0       8     437    1152 
   Size:          8     429     715

  Fragments arranged by size: 

                 715     429       8
 
BtsI GCAGTG_nn'

Cuts at:      0     413    1152 
   Size:        413     739
 
EaeI y'GGCC_r

Cuts at:      0      50     104     161     206     299     386     445     452 
   Size:         50      54      57      45      93      87      59       7

Cuts at:    452     515     647     815     884    1152 
   Size:         63     132     168      69     268

  Fragments arranged by size: 

                 268     168     132      93      87      69      63      59
                  57      54      50      45       7
 
EagI C'GGCC_G

Cuts at:      0      50     445    1152 
   Size:         50     395     707

  Fragments arranged by size: 

                 707     395      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     379     415     622     805    1152 
   Size:        379      36     207     183     347

  Fragments arranged by size: 

                 379     347     207     183      36
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     670    1152 
   Size:        670     482
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     384     564     769    1119    1152 
   Size:        205     179     180     205     350      33

  Fragments arranged by size: 

                 350     205     205     180     179      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     361     688    1152 
   Size:        361     327     464

  Fragments arranged by size: 

                 464     361     327
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     140     141     320     366     708     792     997 
   Size:         14     126       1     179      46     342      84     205

Cuts at:    997    1152 
   Size:        155

  Fragments arranged by size: 

                 342     205     179     155     126      84      46      14
                   1
 
HaeII r_GCGC'y

Cuts at:      0     343     429     609    1152 
   Size:        343      86     180     543

  Fragments arranged by size: 

                 543     343     180      86
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     224     256     562     594    1108    1140    1152 
   Size:        224      32     306      32     514      32      12

  Fragments arranged by size: 

                 514     306     224      32      32      32      12
 
HincII GTy'rAC

Cuts at:      0     906    1152 
   Size:        906     246
 
KasI G'GCGC_C

Cuts at:      0     339    1152 
   Size:        339     813
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     857    1152 
   Size:        857     295
 
MscI TGG'CCA

Cuts at:      0     301     649    1152 
   Size:        301     348     503

  Fragments arranged by size: 

                 503     348     301
 
MslI CAynn'nnrTG

Cuts at:      0     439     655    1152 
   Size:        439     216     497

  Fragments arranged by size: 

                 497     439     216
 
MspA1I CmG'CkG

Cuts at:      0      45     170     575     698     721    1152 
   Size:         45     125     405     123      23     431

  Fragments arranged by size: 

                 431     405     125     123      45      23
 
NaeI GCC'GGC

Cuts at:      0      54      67     100     639    1152 
   Size:         54      13      33     539     513

  Fragments arranged by size: 

                 539     513      54      33      13
 
NarI GG'CG_CC

Cuts at:      0     340    1152 
   Size:        340     812
 
NcoI C'CATG_G

Cuts at:      0     437    1152 
   Size:        437     715
 
NgoMIV G'CCGG_C

Cuts at:      0      52      65      98     637    1152 
   Size:         52      13      33     539     515

  Fragments arranged by size: 

                 539     515      52      33      13
 
NruI TCG'CGA

Cuts at:      0     231    1152 
   Size:        231     921
 
NspI r_CATG'y

Cuts at:      0     225     993    1152 
   Size:        225     768     159

  Fragments arranged by size: 

                 768     225     159
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      45     839    1152 
   Size:         45     794     313

  Fragments arranged by size: 

                 794     313      45
 
PpuMI rG'GwC_Cy

Cuts at:      0      14    1152 
   Size:         14    1138
 
PspOMI G'GGCC_C

Cuts at:      0     140     320     792     842     997    1152 
   Size:        140     180     472      50     155     155

  Fragments arranged by size: 

                 472     180     155     155     140      50
 
PstI C_TGCA'G

Cuts at:      0     420    1152 
   Size:        420     732
 
PvuII CAG'CTG

Cuts at:      0      45     170    1152 
   Size:         45     125     982

  Fragments arranged by size: 

                 982     125      45
 
SanDI GG'GwC_CC

Cuts at:      0      14    1152 
   Size:         14    1138
 
SapI GCTCTTCn'nnn_

Cuts at:      0     379     415    1152 
   Size:        379      36     737

  Fragments arranged by size: 

                 737     379      36
 
SfcI C'TryA_G

Cuts at:      0     416    1152 
   Size:        416     736
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     298    1152 
   Size:        298     854
 
SfoI GGC'GCC

Cuts at:      0     341    1152 
   Size:        341     811
 
SgrAI Cr'CCGG_yG

Cuts at:      0     637    1152 
   Size:        637     515
 
SmaI CCC'GGG

Cuts at:      0    1002    1018    1152 
   Size:       1002      16     134

  Fragments arranged by size: 

                1002     134      16
 
SphI G_CATG'C

Cuts at:      0     993    1152 
   Size:        993     159
 
SrfI GCCC'GGGC

Cuts at:      0    1018    1152 
   Size:       1018     134
 
StyI C'CwwG_G

Cuts at:      0     437    1069    1152 
   Size:        437     632      83

  Fragments arranged by size: 

                 632     437      83
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     174     681    1152 
   Size:        174     507     471

  Fragments arranged by size: 

                 507     471     174
 
XmaI C'CCGG_G

Cuts at:      0    1000    1016    1152 
   Size:       1000      16     136

  Fragments arranged by size: 

                1000     136      16
 
ZraI GAC'GTC

Cuts at:      0     822    1152 
   Size:        822     330
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI     AhdI     AleI     AlfI     ApaI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BglI     BlpI
 Bme1580I     BmrI     BplI     BpmI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BstXI    BstYI     BtgI     BtsI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NruI
     NspI    PflMI    PpuMI   PspOMI     PstI    PvuII    SanDI     SapI
     SfcI     SfiI     SfoI    SgrAI     SmaI     SphI     SrfI     StyI
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BseRI    BsiWI     BsmI    BsmBI
    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SbfI     ScaI    SexAI     SgfI     SmlI    SnaBI     SpeI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmnI
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