(Linear) (Six-base) MAPSORT of: KIEE9503.seq Check: 680 from: 1 to: 1152 KIEE9503.orf With 182 enzymes: SgfI * December 27, 2011 16:40 .. AatII G_ACGT'C Cuts at: 0 824 1152 Size: 824 328 AccI GT'mk_AC Cuts at: 0 242 485 1152 Size: 242 243 667 Fragments arranged by size: 667 243 242 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 769 1152 Size: 384 385 383 Fragments arranged by size: 385 384 383 AfeI AGC'GCT Cuts at: 0 427 607 1152 Size: 427 180 545 Fragments arranged by size: 545 427 180 AhdI GACnn_n'nnGTC Cuts at: 0 238 1152 Size: 238 914 AleI CACnn'nnGTG Cuts at: 0 439 1152 Size: 439 713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 967 1152 Size: 967 185 ApaI G_GGCC'C Cuts at: 0 144 324 796 846 1001 1152 Size: 144 180 472 50 155 151 Fragments arranged by size: 472 180 155 151 144 50 AvaI C'yCGr_G Cuts at: 0 28 93 1000 1016 1152 Size: 28 65 907 16 136 Fragments arranged by size: 907 136 65 28 16 BamHI G'GATC_C Cuts at: 0 872 1152 Size: 872 280 BanI G'GyrC_C Cuts at: 0 339 1152 Size: 339 813 BanII G_rGCy'C Cuts at: 0 144 324 796 846 1001 1121 1152 Size: 144 180 472 50 155 120 31 Fragments arranged by size: 472 180 155 144 120 50 31 BbeI G_GCGC'C Cuts at: 0 343 1152 Size: 343 809 BbsI GAAGACnn'nnnn_ Cuts at: 0 757 1152 Size: 757 395 BglI GCCn_nnn'nGGC Cuts at: 0 298 1027 1152 Size: 298 729 125 Fragments arranged by size: 729 298 125 BlpI GC'TnA_GC Cuts at: 0 955 1099 1152 Size: 955 144 53 Fragments arranged by size: 955 144 53 Bme1580I G_kGCm'C Cuts at: 0 62 144 324 796 846 1001 1152 Size: 62 82 180 472 50 155 151 Fragments arranged by size: 472 180 155 151 82 62 50 BmrI ACTGGGnnnn_n' Cuts at: 0 342 1152 Size: 342 810 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 970 1050 1152 Size: 970 80 102 Fragments arranged by size: 970 102 80 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 564 1119 1152 Size: 205 359 555 33 Fragments arranged by size: 555 359 205 33 BsaHI Gr'CG_yC Cuts at: 0 340 641 821 1152 Size: 340 301 180 331 Fragments arranged by size: 340 331 301 180 BsaWI w'CCGG_w Cuts at: 0 1036 1152 Size: 1036 116 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 562 592 1108 1138 1152 Size: 562 30 516 30 14 Fragments arranged by size: 562 516 30 30 14 BseYI C'CCAG_C Cuts at: 0 236 525 543 717 788 984 1152 Size: 236 289 18 174 71 196 168 Fragments arranged by size: 289 236 196 174 168 71 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 709 963 1152 Size: 64 645 254 189 Fragments arranged by size: 645 254 189 64 BsiEI CG_ry'CG Cuts at: 0 53 229 448 1152 Size: 53 176 219 704 Fragments arranged by size: 704 219 176 53 BsiHKAI G_wGCw'C Cuts at: 0 956 1152 Size: 956 196 Bsp1286I G_dGCh'C Cuts at: 0 62 144 324 796 846 956 1001 1121 Size: 62 82 180 472 50 110 45 120 Cuts at: 1121 1152 Size: 31 Fragments arranged by size: 472 180 120 110 82 62 50 45 31 BspEI T'CCGG_A Cuts at: 0 1036 1152 Size: 1036 116 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 284 863 1152 Size: 284 579 289 Fragments arranged by size: 579 289 284 BsrBI CCG'CTC Cuts at: 0 461 1094 1152 Size: 461 633 58 Fragments arranged by size: 633 461 58 BsrFI r'CCGG_y Cuts at: 0 52 65 98 447 637 1152 Size: 52 13 33 349 190 515 Fragments arranged by size: 515 349 190 52 33 13 BstXI CCAn_nnnn'nTGG Cuts at: 0 174 657 1152 Size: 174 483 495 Fragments arranged by size: 495 483 174 BstYI r'GATC_y Cuts at: 0 872 1152 Size: 872 280 BtgI C'CryG_G Cuts at: 0 8 437 1152 Size: 8 429 715 Fragments arranged by size: 715 429 8 BtsI GCAGTG_nn' Cuts at: 0 413 1152 Size: 413 739 EaeI y'GGCC_r Cuts at: 0 50 104 161 206 299 386 445 452 Size: 50 54 57 45 93 87 59 7 Cuts at: 452 515 647 815 884 1152 Size: 63 132 168 69 268 Fragments arranged by size: 268 168 132 93 87 69 63 59 57 54 50 45 7 EagI C'GGCC_G Cuts at: 0 50 445 1152 Size: 50 395 707 Fragments arranged by size: 707 395 50 EarI CTCTTCn'nnn_ Cuts at: 0 379 415 622 805 1152 Size: 379 36 207 183 347 Fragments arranged by size: 379 347 207 183 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 670 1152 Size: 670 482 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 384 564 769 1119 1152 Size: 205 179 180 205 350 33 Fragments arranged by size: 350 205 205 180 179 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 361 688 1152 Size: 361 327 464 Fragments arranged by size: 464 361 327 EcoO109I rG'GnC_Cy Cuts at: 0 14 140 141 320 366 708 792 997 Size: 14 126 1 179 46 342 84 205 Cuts at: 997 1152 Size: 155 Fragments arranged by size: 342 205 179 155 126 84 46 14 1 HaeII r_GCGC'y Cuts at: 0 343 429 609 1152 Size: 343 86 180 543 Fragments arranged by size: 543 343 180 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 224 256 562 594 1108 1140 1152 Size: 224 32 306 32 514 32 12 Fragments arranged by size: 514 306 224 32 32 32 12 HincII GTy'rAC Cuts at: 0 906 1152 Size: 906 246 KasI G'GCGC_C Cuts at: 0 339 1152 Size: 339 813 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 857 1152 Size: 857 295 MscI TGG'CCA Cuts at: 0 301 649 1152 Size: 301 348 503 Fragments arranged by size: 503 348 301 MslI CAynn'nnrTG Cuts at: 0 439 655 1152 Size: 439 216 497 Fragments arranged by size: 497 439 216 MspA1I CmG'CkG Cuts at: 0 45 170 575 698 721 1152 Size: 45 125 405 123 23 431 Fragments arranged by size: 431 405 125 123 45 23 NaeI GCC'GGC Cuts at: 0 54 67 100 639 1152 Size: 54 13 33 539 513 Fragments arranged by size: 539 513 54 33 13 NarI GG'CG_CC Cuts at: 0 340 1152 Size: 340 812 NcoI C'CATG_G Cuts at: 0 437 1152 Size: 437 715 NgoMIV G'CCGG_C Cuts at: 0 52 65 98 637 1152 Size: 52 13 33 539 515 Fragments arranged by size: 539 515 52 33 13 NruI TCG'CGA Cuts at: 0 231 1152 Size: 231 921 NspI r_CATG'y Cuts at: 0 225 993 1152 Size: 225 768 159 Fragments arranged by size: 768 225 159 PflMI CCAn_nnn'nTGG Cuts at: 0 45 839 1152 Size: 45 794 313 Fragments arranged by size: 794 313 45 PpuMI rG'GwC_Cy Cuts at: 0 14 1152 Size: 14 1138 PspOMI G'GGCC_C Cuts at: 0 140 320 792 842 997 1152 Size: 140 180 472 50 155 155 Fragments arranged by size: 472 180 155 155 140 50 PstI C_TGCA'G Cuts at: 0 420 1152 Size: 420 732 PvuII CAG'CTG Cuts at: 0 45 170 1152 Size: 45 125 982 Fragments arranged by size: 982 125 45 SanDI GG'GwC_CC Cuts at: 0 14 1152 Size: 14 1138 SapI GCTCTTCn'nnn_ Cuts at: 0 379 415 1152 Size: 379 36 737 Fragments arranged by size: 737 379 36 SfcI C'TryA_G Cuts at: 0 416 1152 Size: 416 736 SfiI GGCCn_nnn'nGGCC Cuts at: 0 298 1152 Size: 298 854 SfoI GGC'GCC Cuts at: 0 341 1152 Size: 341 811 SgrAI Cr'CCGG_yG Cuts at: 0 637 1152 Size: 637 515 SmaI CCC'GGG Cuts at: 0 1002 1018 1152 Size: 1002 16 134 Fragments arranged by size: 1002 134 16 SphI G_CATG'C Cuts at: 0 993 1152 Size: 993 159 SrfI GCCC'GGGC Cuts at: 0 1018 1152 Size: 1018 134 StyI C'CwwG_G Cuts at: 0 437 1069 1152 Size: 437 632 83 Fragments arranged by size: 632 437 83 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 174 681 1152 Size: 174 507 471 Fragments arranged by size: 507 471 174 XmaI C'CCGG_G Cuts at: 0 1000 1016 1152 Size: 1000 16 136 Fragments arranged by size: 1000 136 16 ZraI GAC'GTC Cuts at: 0 822 1152 Size: 822 330 Enzymes that do cut: AatII AccI AcuI AfeI AhdI AleI AlfI ApaI AvaI BamHI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmrI BplI BpmI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BstXI BstYI BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PflMI PpuMI PspOMI PstI PvuII SanDI SapI SfcI SfiI SfoI SgrAI SmaI SphI SrfI StyI XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AflIII AgeI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SgfI SmlI SnaBI SpeI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI